System and method for separating and purifying flavones and polysaccharide from oldenlandia diffusa

Abstract

The invention discloses a system for separating and purifying flavones and polysaccharide from oldenlandia diffusa. The system is characterized by comprising an ultrasonic extraction device, a pre-filtering device and ceramic membrane equipment which are connected in sequence, wherein a dialyzate outlet of the ceramic membrane equipment is connected with ultrafiltration membrane equipment; a concentrated liquid outlet of the ultrafiltration membrane equipment is connected with a first vacuum concentration and spraying device; the dialyzate outlet is connected with a continuous ion exchange andspectrum separation device; the continuous ion exchange and spectrum separation device and the dialyzate outlet are sequentially connected with membrane concentration equipment and a second vacuum concentration device and spraying device. The invention further discloses a method for separating and purifying flavones and polysaccharide from oldenlandia diffusa. The system is simple in process, capable of achieving separation and purification of flavones and polysaccharide from oldenlandia diffusa rapidly at a time, short in production cycle, possible in continuous material feeding and discharge, stable in operation, high in product content, full-automatic in operation, capable of saving a great amount of labor and materials and applicable to industrial popularization.

Description

technical field [0001] The invention relates to the technical field of separation of effective components of traditional Chinese medicine, in particular to a system and method for separating and purifying flavonoids and polysaccharides of Hedyotis diffusa by using membrane separation and continuous ion exchange technology. Background technique [0002] Hedyotis diffusa is an annual scattered herb with bitter, sweet and cold nature. It has the functions of clearing away heat, promoting dampness and detoxification. It is used to treat dysentery, urethritis, jaundice, appendicitis, sores, swelling and pain, and mosquito bites. It is a traditional Chinese medicine prescription. One of the commonly used drugs in China. Modern phytochemical studies have shown that Hedyotis diffusa contains a variety of chemical components, including flavonoids, anthraquinones, sterols, polysaccharides, terpenoids and aliphatic compounds. In clinical treatment, Hedyotis diffusa is widely used, and...

AI Technical Summary

Problems solved by technology

[0004] Patent Publication No. CN101496845A provides an extract of Hedyotis diffusa and its separation and preparation method, extracting and separating four substances such as cartilinic acid and cartilin in Hedyotis, and extracting them by water extraction After decompression evaporation to make the extract, alcohol precipitation, filtration, re-concentration, re-alcohol precipitation and centrifuge centrifugation, and finally enter the 3000-6000D ultrafiltration membrane system system, and then enter the macroporous adsorbent and industrial chromatographic separation system. This process has the characteristics of high product components, but the disadvantage of this method is that the extraction process uses highly dangerous ethanol, and in order to obtain a clear filtrate, the pretreatment process needs to go through multiple centrifugation, alcohol precipitation, standing and other processes, especially When using adsorbents to separate flavonoids, the resin column must be disconnected after all the filtrate has passed through, and then perform operations such as water washing and alcohol analysis. The overall process takes a long time and the process is cumbersome and complicated. The production cycle from extraction and preparation to final Hedyotis diffusa extract product is as long as 10 days”

[0005] Patent Publication No. CN104173526A discloses a preparation method of Hedyotis diffusa flavonoids, which adopts the method of vacuum evaporation concentration and polyamide resin column chromatography separation and purification. The active ingredients are not processed, which is a great waste. In the separation and purification process of polyamide resin column chromatography, it is also necessary to wait for all the filtrate to pass through before the resin can be cleaned and analyzed. The overall process takes a long time and the process is cumbersome. complex

[0006] There are no relevant reports on the method of separating and purifying flavonoids and polysaccharides from Hedyotis diffusa by combining membrane separation technology and continuous ion exchange technology

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