The invention relates to a method of using
stevia rebaudiana for manufacturing pure natural high-purity
stevioside; the method comprises the following steps of: taking the weight of the
stevia rebaudiana as the basis of the addition amount of all matters,
grinding and sieving after air-
drying the
stevia rebaudiana, and then collecting the sieved stevia rebaudiana
powder; adding pure water, and then adding
cellulase for breaking
cell walls and simultaneously heating a solution; adopting an ultrasonic wave continuous countercurrent extraction technology for extracting, filtering and separating an extracting solution, adding a solution obtained by squeezing filtered residue into the extracting solution, wherein the filtered residue is used for
fermentation so as to obtain
biomass, using a membrane device for purifying and removing impurities of filtrate; using an
ultrafiltration membrane with the molecular weight cutoff of 6000Da1 for removing macromolecular matters of the filtrate; and using a nano-
filtration membrane with the molecular weight cutoff of 1200 Da1 for moving micro-molecular matters of the filtrate and concentrating; using a multifunctional simulating analogue
moving bed separation system for absorbing a concentrated solution, using an
alcohol solution for
elution, using the nano-
filtration membrane with the molecular weight cutoff of 600Da1 for filtering the eluted solution so as to remove
alcohol or the micro-molecular matters, concentrating by two times, carrying out the
spray drying on an obtained concentrated solution so as to obtain the high-purity
stevioside.