Manufacturing method of pure natural high-purity stevioside

Abstract

The invention relates to a method of using stevia rebaudiana for manufacturing pure natural high-purity stevioside; the method comprises the following steps of: taking the weight of the stevia rebaudiana as the basis of the addition amount of all matters, grinding and sieving after air-drying the stevia rebaudiana, and then collecting the sieved stevia rebaudiana powder; adding pure water, and then adding cellulase for breaking cell walls and simultaneously heating a solution; adopting an ultrasonic wave continuous countercurrent extraction technology for extracting, filtering and separating an extracting solution, adding a solution obtained by squeezing filtered residue into the extracting solution, wherein the filtered residue is used for fermentation so as to obtain biomass, using a membrane device for purifying and removing impurities of filtrate; using an ultrafiltration membrane with the molecular weight cutoff of 6000Da1 for removing macromolecular matters of the filtrate; and using a nano-filtration membrane with the molecular weight cutoff of 1200 Da1 for moving micro-molecular matters of the filtrate and concentrating; using a multifunctional simulating analogue moving bed separation system for absorbing a concentrated solution, using an alcohol solution for elution, using the nano-filtration membrane with the molecular weight cutoff of 600Da1 for filtering the eluted solution so as to remove alcohol or the micro-molecular matters, concentrating by two times, carrying out the spray drying on an obtained concentrated solution so as to obtain the high-purity stevioside.

Description

technical field [0001] The invention relates to a production method of pure natural stevioside, in particular to a method for preparing a kind of pure natural high-purity stevioside with stevia as raw material. Background technique [0002] Stevia rebaudiana, also known as stevia, sweet grass, honey chrysanthemum, sweet tea, etc., is native to South America and is a tropical perennial herb of the Compositae family. Its leaves are very sweet. Since ancient times, local residents have used the leaves to make sweet tea drinks. The sweetness is 300 times that of sucrose, and it is the sweetest natural sugar known so far. [0003] In recent years, as a new sugar source and food additive, stevioside has attracted the attention of countries all over the world, and the domestic and foreign market demand for stevioside has also shown a growing trend. China has become the world's largest stevia producer and exporter, accounting for more than 80% of the global stevia market. From the...

AI Technical Summary

Problems solved by technology

However, single-column adsorption and ion exchange require a large column volume, a long time for the liquid to flow through the column, a large volume of solution, and a large amount of eluent, resulting in extremely high operating costs.