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Method for extracting, separating and purifying honokiol and magnolol from magnolia officinalis

A technology for separating and purifying honokiol, applied in the field of honokiol and honokiol extraction, can solve the problems of cumbersome operation process, lack of theoretical guidance, difficult selection of solvent system, etc., and achieve the effect of low comprehensive cost

Inactive Publication Date: 2015-11-04
XUZHOU MEDICAL COLLEGE
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Problems solved by technology

According to literature reports, the methods for separating and purifying magnolia lignin mainly include repeated silica gel column chromatography and high-speed countercurrent chromatography. The operation process of silica gel column chromatography is cumbersome, the recovery rate is low, and chloroform, benzene, etc. are often used. organic solvents; the choice of solvent system in high-speed countercurrent chromatography is difficult and lacks theoretical guidance

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  • Method for extracting, separating and purifying honokiol and magnolol from magnolia officinalis
  • Method for extracting, separating and purifying honokiol and magnolol from magnolia officinalis
  • Method for extracting, separating and purifying honokiol and magnolol from magnolia officinalis

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Embodiment

[0025] Embodiment: the method for extracting, separating and purifying honokiol and magnolol from Magnolia officinalis, its steps are:

[0026] (1) Extraction of Magnolia officinalis Lignin: Pulverize Magnolia officinalis medicinal material, ultrasonically extract 3 times with 10 times the amount of 80% ethanol, each time for 45 minutes, filter, combine the extracts, and concentrate under reduced pressure to obtain a crude extract.

[0027] (2) Separation and analysis of magnolia lignin: use analytical high performance liquid chromatography to separate and analyze the composition of the crude extract, and the chromatographic column is C 18 Column (250×4.6mm I.D., 5μm), the mobile phase is methanol-water (73:27, V / V); the flow rate is 1.0mL / min, the detection wavelength is 254nm, and the column temperature is room temperature.

[0028] (3) Separation and purification of magnolia lignin: use a semi-preparative high-performance liquid chromatograph to separate and purify the comp...

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Abstract

The invention discloses a method for extracting, separating and purifying honokiol and magnolol from magnolia officinalis, and relates to the technical field of the extraction of honokiol and magnolol. The method comprises the steps: firstly ultrasonically extracting lignin components from magnolia officinalis by utilizing an organic solvent; then separating and analyzing constituents of an extract by utilizing an analysis-type high-efficiency liquid phase chromatograph; then separating and purifying components in the extract by utilizing a semi-prepared high-efficiency liquid phase chromatograph to obtain two compounds, i.e. honokiol and magnolol; and finally determining the purity of the compounds by utilizing a high-efficiency liquid phase chromatography. The structure of each compound is identified according to nuclear magnetic resonance spectroscopy analysis, the target compound prepared by the method is high in purity and extremely low in content of impurities, the process flow is environment-friendly, no severe harm on the environment is produced, and the comprehensive cost is low.

Description

technical field [0001] The invention relates to the technical field of honokiol and magnolol extraction, in particular to a method for extracting, separating and purifying honokiol and magnolol from magnolia bark. Background technique [0002] Magnolia officinalis is the dry bark, root bark or branch bark of Magnolia officinalis Rehd.et Wils. It is warm in nature, bitter in taste, pungent, and has the effects of drying dampness and eliminating phlegm, lowering qi and eliminating fullness. It is used for Treat diseases such as stagnation of dampness in the middle, vomiting and diarrhea due to removal of swelling, abdominal distension and constipation, phlegm retention, dyspnea and cough. Pharmacological studies have shown that magnolia officinalis has anti-fungal, anti-viral, anti-allergic, anti-ulcer, anti-allergic, anti-cancer, anti-platelet aggregation, antihypertensive, liver-protecting, and gastrointestinal motility effects, and its main active ingredient is lignin , in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C39/21C07C37/70
CPCC07C37/004C07C39/21
Inventor 汤道权孙世安纪海侠杨方秀董露露栗政
Owner XUZHOU MEDICAL COLLEGE
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