Application of melatonin in reducing cadmium accumulation in plants

A technology of melatonin and phytosomes, applied in the field of reducing cadmium accumulation in plants, can solve the problems of not being suitable for large-scale promotion, slow effect, long treatment cycle, etc., to achieve alleviating damage, reducing cadmium content, and high-efficiency cadmium content Effect

Active Publication Date: 2015-08-12
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Compared with other methods, the phytoremediation method has low cost, little damage to the soil, and no secondary pollution. However, the treatment cycle is long and the effect is slow, so it is not suitable for large-scale promotion. It is more suitable as an auxiliary means

Method used

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  • Application of melatonin in reducing cadmium accumulation in plants
  • Application of melatonin in reducing cadmium accumulation in plants
  • Application of melatonin in reducing cadmium accumulation in plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Plant material

[0033] Tomato (Solanum lycopersicum L.cv. Ailsa Craig) seeds were soaked in warm water at 65°C to accelerate germination and sowed in a mixed substrate containing peat and vermiculite (3:1, V:V) or perlite, and watered according to the moisture content of the substrate after unearthed. Water keeps the substrate moist. After the first true leaf appeared, it was moved into a hydroponic box (40cm×25cm×15cm), with 6 tomato seedlings in each hydroponic box, and the sponges were fixed. The nutrient solution adopts Hoagland's formula, and the nutrient solution is replaced once a week.

[0034] (2) Preparation of melatonin preparation

[0035] Set up 5 melatonin preparations with concentration gradients (25 μM, 50 μM, 100 μM, 250 μM, 500 μM). The composition is as follows: 98% melatonin (Aladdin, Shanghai Jingchun Biochemical Technology Co., Ltd.), ethanol, water. The melatonin preparation is prepared according to the following steps: first mix 118.5 mg...

Embodiment 2

[0044] Steps (1)~(2) are the same as Example 1, spray once every 3 days in step (3), spray 5 times altogether, carry out cadmium content measurement according to Example step (4), the result shows that can reach the embodiment. 1 processing effect.

Embodiment 3

[0046] The steps are the same as in Example 1, replace the tomato with lettuce, and measure the cadmium content according to the step (4) of the example, and the results show that the treatment effect of Example 1 can be basically achieved.

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Abstract

The invention discloses an application of melatonin in reducing cadmium accumulation in plants. The application includes the following steps: (1) mixing melatonin and additives to form aqueous solution; (2) spraying the aqueous solution on plant leaf surfaces under cadmium stress or filling the aqueous solution in nutrient solution or soil where plants grow. The melatonin can quickly and efficiently reduce cadmium content in plant leaves, change distribution of cadmium ions in plant cells, and relieve damage of cadmium to cells; in addition, the melatonin is simple to prepare and convenient to use, does not cause product and environment pollution, does not generate phytotoxicity easily and has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of melatonin application, in particular to the application of melatonin to reduce cadmium accumulation in plants. Background technique [0002] Cadmium (cadmium, Cd) is one of the most toxic heavy metals. It cannot be degraded by microorganisms in the soil, and can only be transformed between the storage forms due to different conditions. The ability of cadmium to migrate from soil to plants is better than that of other non- Essential elements are stronger and are easily absorbed and accumulated by plants. If they exceed a certain limit, they will seriously affect the yield and quality of crops, and they will be enriched through the food chain, endangering human health. The toxicity of cadmium to plants is mainly manifested in the form of chlorosis and curling of leaves, and the growth retardation of roots and stems; in terms of physiology, it is mostly manifested in the inhibition of photosynthesis, causing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06A01N43/38A01P21/00
CPCA01G7/06A01N43/38
Inventor 周杰蔡淑钰哈桑·穆罕默德喻景权
Owner ZHEJIANG UNIV
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