Application of melatonin in reducing cadmium accumulation in plants
A technology of melatonin and phytosomes, applied in the field of reducing cadmium accumulation in plants, can solve the problems of not being suitable for large-scale promotion, slow effect, long treatment cycle, etc., to achieve alleviating damage, reducing cadmium content, and high-efficiency cadmium content Effect
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Embodiment 1
[0032] (1) Plant material
[0033] Tomato (Solanum lycopersicum L.cv. Ailsa Craig) seeds were soaked in warm water at 65°C to accelerate germination and sowed in a mixed substrate containing peat and vermiculite (3:1, V:V) or perlite, and watered according to the moisture content of the substrate after unearthed. Water keeps the substrate moist. After the first true leaf appeared, it was moved into a hydroponic box (40cm×25cm×15cm), with 6 tomato seedlings in each hydroponic box, and the sponges were fixed. The nutrient solution adopts Hoagland's formula, and the nutrient solution is replaced once a week.
[0034] (2) Preparation of melatonin preparation
[0035] Set up 5 melatonin preparations with concentration gradients (25 μM, 50 μM, 100 μM, 250 μM, 500 μM). The composition is as follows: 98% melatonin (Aladdin, Shanghai Jingchun Biochemical Technology Co., Ltd.), ethanol, water. The melatonin preparation is prepared according to the following steps: first mix 118.5 mg...
Embodiment 2
[0044] Steps (1)~(2) are the same as Example 1, spray once every 3 days in step (3), spray 5 times altogether, carry out cadmium content measurement according to Example step (4), the result shows that can reach the embodiment. 1 processing effect.
Embodiment 3
[0046] The steps are the same as in Example 1, replace the tomato with lettuce, and measure the cadmium content according to the step (4) of the example, and the results show that the treatment effect of Example 1 can be basically achieved.
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