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Preparation method for extracting oridonin from rabdosia

A technology of Rubescensin A and Rubescens, which is applied in the direction of organic chemistry, can solve the problems of increased column load, higher production costs, and lack of quality control standards, so as to reduce concentration and content and reduce production costs , The effect of shortening the extraction time

Inactive Publication Date: 2006-07-26
陈俊辉 +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Dong Lingcao buccal tablets that have entered the market at present are made by traditional Chinese medicine processing methods. The disadvantages are the lack of stricter quality control standards, the curative effect is not reliable, and there is no effect on the active ingredients in Dong Lingcao. extract and purify
Because the content of oridonin is relatively low at this time, the load on the chromatographic column is increased, and the production cost is also increased. Therefore, it is not suitable for industrial scale production needs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation method of oridonin

[0027] Get 30kg of the raw material of Rubescensia GAP standard, crush it to 40-80 meshes, add 95% boiling ethanol solution equivalent to 6 times the amount of medicinal materials, and extract for 30 minutes to obtain Rubescensus A containing 10.5% Rubescensin Extract 1kg. Desorb with a mixed solvent of ethyl acetate and petroleum ether (2:1) for 2 hours, then desorb with a mixed solvent of petroleum ether and gasoline (1:1.5) for 3 hours, and recover the stripped solution. Add the recovered desorption liquid into a rotary evaporator, and evaporate the petroleum ether and gasoline therein. After being treated with ethyl acetate, a paste containing 41.2% oridonin was obtained, treated with 2 times of ethanol, and placed in a cool place. Repeat the above steps four times (a total of 120kg of grass), after the four pastes are combined, mix it with 100-200 mesh silica gel at a ratio of 1:1.5, and dry in the shade. Take 3 times of fres...

Embodiment 2

[0029] The preparation method of oridonin

[0030] Purchased 100kg of GAP Radix radix L., crushed it to 40-80 mesh, added 6 times the amount of 95% ethanol solution, and extracted for 30 minutes to obtain about 3.1 kg of Radix ernium extract containing 10.8%. Desorb with a mixed solvent of ethyl acetate and petroleum ether (1.5:1) for 2 hours, then perform a second desorption with a mixed solvent of petroleum ether and gasoline (1:1) for 2 hours, and recover the stripped solution. Add the recovered desorption liquid into a rotary evaporator, and evaporate the petroleum ether and gasoline therein. After being treated with ethyl acetate, a paste containing 36.3% oridonin was obtained, treated with 2 times of ethanol, and placed in a cool place. Mix the paste obtained above with 100-200 mesh silica gel at a ratio of 1:1.5, and dry in the shade. Take 3 times of fresh silica gel, mix and pack the column. The diameter-to-height ratio of the used column chromatography separation c...

Embodiment 3

[0032] The preparation method of oridonin

[0033] Purchased 100kg of GAP Radix radix L., crushed to 40-80 meshes, added 8 times the amount of 70% ethanol solution, and extracted for 50 minutes to obtain 12% Radix Radix Radix Radix Extract. Desorb with a mixed solvent of ethyl acetate and petroleum ether (1.5:1) for 3 hours, then perform a second desorption with a mixed solvent of petroleum ether and gasoline (1:1) for 3 hours, and recover the stripped solution. Add the recovered desorption liquid into a rotary evaporator, and evaporate the petroleum ether and gasoline therein. After being treated with ethyl acetate, a paste containing 40% oridonin was further obtained, treated with 2 times of ethanol, and placed in a cool place. Mix the paste obtained above with 100-200 mesh silica gel at a ratio of 1:1, and dry in the shade. Take 3 times of fresh silica gel, mix and pack the column. The diameter-to-height ratio of the used column chromatography separation column is 1:5. ...

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PUM

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Abstract

The invention discloses a Method for preparation of rabdosia form element which is distilled from rabdosia, adopting 95% ethanol as extracting solvent, the warming reflux extraction time is 30-50min, then after extracting by using ethyl acetate ethyl acetate: ligroine, ligroine: petrol mixed solvent of different proportion, the extract which containing rabdosia form element will be aquired; blending by column chromatographic silica gel and loading in short range for separating and purifying. The process of this invention is easy and feasible, it is new method to separate and purify rabdosia form element and suitable for mass production, extraction. Separation, purifying and reduction in production costs can be realized fleetly, the purity of obtained rabdosia form element is above 95%.

Description

technical field [0001] The invention relates to a method for extracting and purifying trace effective components of plants, and more specifically designs a method for separating and purifying oridonin. technical background [0002] Dongling grass is also known as ice grass. Rubescens is used in the folk for anti-inflammatory, antibacterial, analgesic, and treatment of esophageal cancer, gastric cancer, and liver cancer, and can also inhibit the proliferation of esophageal epithelium. The Dong Lingcao buccal tablets that have entered the market at present are made by traditional Chinese medicine processing methods. The disadvantages are the lack of stricter quality control standards, the curative effect is not reliable, and there is no effect on the active ingredients in Dong Lingcao. Carry out extraction separation and purification. Moreover, since Rubescensin A and B belong to diterpenoids and are difficult to dissolve in water, it is almost difficult for general dosage f...

Claims

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Application Information

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IPC IPC(8): C07D493/08
Inventor 陈俊辉常贵生王少彬常菁朱晨霞
Owner 陈俊辉
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