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Method for preparing walnut green husk polyphenol from fresh walnut green husks

A technology of walnut green husk and fresh walnut, which is applied in the field of preparation of walnut green husk polyphenols, can solve the problems of unfavorable natural drying, high drying and extraction temperature, and heat consumption, and achieve the effects of avoiding adverse effects, low preparation temperature, and reducing oxidation

Inactive Publication Date: 2016-06-29
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The above methods all use dry and crushed walnut green skin powder, extract with water or ethanol or methanol water, and then separate and purify; fresh walnut green skin needs to be dried first, which requires heat consumption, long natural drying time, and the weather in the walnut harvest season is extremely bad. Conducive to natural drying, extraction and purification require a large amount of organic solvents, high drying and extraction temperatures, and the effective components are easily destroyed

Method used

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  • Method for preparing walnut green husk polyphenol from fresh walnut green husks
  • Method for preparing walnut green husk polyphenol from fresh walnut green husks
  • Method for preparing walnut green husk polyphenol from fresh walnut green husks

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Fresh walnuts are peeled off fresh walnut green skins, and fresh walnut green skins are squeezed with a juicer. The juice is centrifuged at 3000 rpm for 20 minutes, and then the upper liquid and the lower residue are separated. The pH value of the upper liquid is adjusted to 3 and mixed. The polyphenol concentration of the sample solution is 2mg / mL, the sample volume is 1.6BV, and the sample flow rate is 2BV / h. Add it into a chromatography column with 1000 grams of AB-8 resin inside, add water to the chromatography column, and wash with water After the liquid flowing out from the bottom of the chromatography column is colorless, elute the chromatography column with ethanol with a volume fraction of 65%, the elution flow rate is 1BV / h, and the elution volume is 2.10BV, and the eluted liquid flowing out from the bottom of the chromatography column is collected. solution, the eluate was concentrated in vacuum to a solid solution content of 52% of the solution at a vacuum de...

Embodiment 2

[0038] Fresh walnuts are peeled off fresh walnut green skins, fresh walnut green skins are squeezed with a juicer, the juice is centrifuged at 4500 rpm for 10 minutes, and then the upper liquid and the lower residue are separated, the pH value of the upper liquid is adjusted to 4, and the above The polyphenol concentration of the sample liquid is 3mg / mL, the sample volume is 1.6BV, and the sample flow rate is 2BV / h, and it is added to the chromatographic column containing 2000 grams of AB-8 resin, and water is added to the chromatographic column, and washed with water After the liquid flowing out from the bottom of the chromatography column is colorless, elute the chromatography column with ethanol with a volume fraction of 70%, the elution flow rate is 2BV / h, and the elution volume is 2.10BV, and the eluted liquid flowing out from the bottom of the chromatography column is collected. solution, the eluate was vacuum-concentrated to a solid solution content of 54% of the solutio...

Embodiment 3

[0042] Fresh walnuts are peeled and fresh walnut green skins are extracted, fresh walnut green skins are squeezed with a juicer, the juice is centrifuged at 4000 rpm for 15 minutes, and then the upper liquid and the bottom residue are separated, the pH value of the upper liquid is adjusted to 3.6, and the above The polyphenol concentration of the sample liquid is 2.5mg / mL, the sample volume is 1.6BV, and the sample flow rate is 2BV / h, and it is added to a chromatographic column with 1500 grams of AB-8 resin inside, and water is added to the chromatographic column. After washing until the liquid flowing out from the bottom of the chromatography column is colorless, elute the chromatography column with ethanol with a volume fraction of 75%, the elution flow rate is 1-2BV / h, and the elution volume is 2.10BV, and collect the liquid flowing out from the bottom of the chromatography column The eluate, the eluate was vacuum-concentrated to a solid solution content of 55% of the soluti...

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Abstract

The invention relates to a method for preparing walnut green husk polyphenol from fresh walnut green husks and belongs to the technical field of walnut green husk polyphenol preparation. The technological process of the method includes the steps that fresh walnut green husks are juiced, centrifuged and subjected to solid and liquid separation, after the pH value of liquid is adjusted to be 3-4, the liquid is added into a chromatographic column in which AB-8 type resin is contained with the polyphenol concentration of the sample adding liquid being 2-3 mg / mL, pH of the sample adding liquid being 3-4, the volume of added samples being 1.6 BV and the flowing speed of the added samples being 2 BV / h, washing is performed with water, after liquid flowing out of the lower portion of the chromatographic column is colorless, elution is performed with a 65-75% ethyl alcohol, the flowing speed of elution is 1-2 BV / h, and the volume of elution is 2.10 BV. 65-75% of ethyl alcohol eluant is collected, vacuum concentration is performed, and the walnut green husk polyphenol with the content being 53% is obtained through vacuum drying. Compared with a traditional method, the steps of drying the walnut green husks and extracting the walnut green husks with an organic solvent are reduced, the contact time between the green husks and air is shortened, oxidization of the walnut green husk polyphenol is reduced, polyphenol substances are effectively protected, and the consumption of heat and the organic solvent is remarkably reduced.

Description

technical field [0001] The invention relates to a method for preparing walnut green peel polyphenols from fresh walnut green peel, belonging to the technical field of preparation of walnut green peel polyphenols. Background technique [0002] Walnut green husk (walnut green husk) has another name called walnut blue dragon, is the thick green exocarp of walnut outer one deck. Walnut green peel has a bitter taste, astringent nature, and slightly cold. It has the effects of clearing away heat and detoxification, expelling wind and treating ringworm, relieving pain and dysentery. Modern pharmacological studies have shown that walnut green peel has anti-tumor, analgesic, antibacterial and anti-inflammatory activities. Walnut green skin is rich in polyphenols, polyphenols have anti-oxidation, anti-radiation, anti-virus, lipid-lowering, antibacterial, anti-aging, improve the body's immunity, inhibit hyperlipidemia total cholesterol (TC), triglyceride (TG) Increase and inhibit hepa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/52A61K131/00
CPCA61K36/52A61K2236/31A61K2236/51A61K2236/53A61K2236/55
Inventor 赵声兰陈丹张旭赵庆宇婧曹丽娟
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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