Solution for improving recovery rate of coccidian oocyst from chicken tissues and preparation method of solution

Abstract

The invention relates to a solution for improving the recovery rate of coccidian oocyst from chicken tissues and a preparation method of the solution. The solution consists of the following components in parts by weight: 5-30 parts of aluminium polychlorid, 5-30 parts of ethylenediamine tetraacetic acid tetrasodium salt, 0.1-0.5 part of guar gum hydroxypropyl trimethyl ammonium chloride, 5-30 parts of sodium hypochlorite and 10-90 parts of sterilized water. According to the solution provided by the invention, coccidian oocysts in the tissues are separated and purified by virtue of such actions as flocculation, adsorption, digestion and the like, so that in comparison with a saturated saline flotation method, a sucrose solution flotation method, a saturated magnesium sulfate flotation method and the like, the solution has the advantages that the yield of oocyst collected from faeces is greatly improved, the oocyst flotation rate is greatly improved, the impurity proportion is effectively reduced, and the separation and purification speeds are high, and the solution is applicable to separation and purification of specimens which are affected at strong and weak levels.

Description

technical field [0001] The invention belongs to the field of veterinary biological products, in particular to a solution for improving the recovery rate of coccidia oocysts in chicken tissues and a preparation method thereof Background technique [0002] Poultry coccidiosis is the most harmful parasitic disease in the intensive and large-scale situation. Under the advocacy of food safety at home and abroad, it has gradually changed from drug treatment to pre-disease prevention. Therefore, the research and development and production of coccidiosis vaccines have become a major issue in the livestock industry to fight against poultry. The new favorite of coccidiosis. At present, most of the coccidia oocysts obtained from domestic vaccines are obtained from feces by means of saturated brine flotation, sucrose solution flotation, saturated magnesium sulfate solution flotation, chromium sulfuric acid separation and other methods. Coccidia oocysts are separated from impurities, bu...

AI Technical Summary

Problems solved by technology

At present, most of the coccidia oocysts obtained from domestic vaccines are obtained from feces by means of saturated brine flotation, sucrose solution flotation, saturated magnesium sulfate solution flotation, chromium sulfuric acid separation and other methods. Coccidia oocysts are separated from impurities, but the separated oocysts are often mixed with a small amount of impurities similar to coccidia oocysts, and some oocysts are embedded by crude fiber and fat particles in some feeds and are difficult to separate

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