Exosome processing chip and method

A technology for processing chips and processing methods, which is applied in the fields of biomass post-processing, biological material pre-treatment, electric/wave energy processing enzymes, etc., which can solve the problems of destroying exosomes, uneven particle size, low purity and recovery rate, etc.

Active Publication Date: 2017-12-08
SHENZHEN HUIXIN LIFE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of PEG to precipitate exosomes has problems such as low purity and recovery, more impurities, uneven particle size, and difficult to remove polymers. Mechanical force or chemical additives such as Tween-20 will destroy exosomes, etc.

Method used

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  • Exosome processing chip and method
  • Exosome processing chip and method
  • Exosome processing chip and method

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Embodiment Construction

[0034] see figure 1 , the structural diagram of the first preferred embodiment of the exosome processing chip 100 of the present invention, the exosome processing chip 100 is used for separating and purifying exosomes. The exosome processing chip 100 includes a substrate 10 , two opposite electrodes 21 , 22 clamped from two ends of the substrate 10 , and a gel layer 30 disposed on the upper surface of the substrate 10 . The gel layer 30 is used to separate impurities in the exosome mixture.

[0035] It can be understood that the exosomes exist in body fluids, such as, but not limited to, blood, saliva, urine or breast milk. The exosome has a special structure, such as including wrapped DNA fragments, small RNA and polypeptide, so that the exosome is electrically neutral.

[0036] The substrate 10 is, for example, but not limited to one or a combination of glass, quartz wafer, silicon wafer, or polymer wafer.

[0037] The gel layer 30 is a conductive porous material. The ge...

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Abstract

The invention provides an exosome processing chip used for separating and purifying exosome. The exosome processing chip comprises a substrate, two electrodes and a gel layer, wherein the two electrodes are arranged at two ends of the substrate and are used for providing positive and negative voltages; the gel layer is arranged on the upper surface of the substrate and is used for separating impurities in an exosome mixed solution. The gel layer is provided with at least one sample loading groove, and the at least one sample loading groove is used for storing the exosome obtained by separating the exosome mixed solution and carrying out purifying. The invention further relates to an exosome processing method using the exosome processing chip. Therefore, the exosome processing chip and the exosome processing method provided by the invention can be used for rapidly separating and purifying the exosome at high throughput.

Description

technical field [0001] The invention relates to an exosome processing chip and a processing method. Background technique [0002] Exosomes are circular single-layer membrane structures with a diameter of 40-100 nm, which can be released by many types of cells in the body and are widely distributed in body fluids such as saliva, plasma, milk, and urine. Exosomes can carry a variety of proteins, mRNA, and miRNA, and participate in processes such as cell communication, cell migration, angiogenesis, and tumor cell growth. Therefore, the isolation and purification of exosomes has attracted more and more attention. [0003] At present, exosome-based processing methods include differential centrifugation, density gradient centrifugation, magnetic bead immunoassay, and PEG-base precipitation method. [0004] Although the differential centrifugation method is simple to operate and obtains a large number of exosomes, the processing process is time-consuming, and the recovery rate is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/42C12N5/07C12N13/00
CPCC12N5/06C12N13/00
Inventor 陈欲超
Owner SHENZHEN HUIXIN LIFE TECH CO LTD
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