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Cultivation method for increasing yield of pinctada martensii pearl particles

A technology of Pinctada martensii and its cultivation method, which is applied in the field of cultivation of Pinctada martensii, can solve the problems of difficulty in meeting market demand and low output, achieve good economic and social benefits, and increase output

Inactive Publication Date: 2019-04-12
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Particulate pearls are mainly natural pearls, and the output is low, which is difficult to meet the market demand

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A method for cultivating pearls of Pinctada martensii microparticles for improving production, comprising the steps of:

[0017] (1) Preparation of nacre powder: select the brightly colored shells of Pinctada martensii nacre, remove the cuticle and prism layer of the shell, pulverize and sieve to obtain nacre powder with a particle size of 50 microns;

[0018] (2) Preparation of mantle slices: select 1.0-year-old Pinctada martensii as donor shellfish to make mantle slices, cut mantle strips at 1 mm above and below the mantle color line, and cut the mantle strips into 0.5 Small pieces of tissue in mm;

[0019] (3) Cell suspension preparation: at 25°C, digest tissue pieces with trypsin at a concentration of 100 units / ml for 1 hour, centrifuge at 1,500 rpm for 5 minutes, and retain the supernatant; centrifuge at 3,000 rpm for 10 Minutes, use sterilized seawater to suspend the cells to obtain a cell suspension;

[0020] (4) Preparation of mixed liquid from nacre powder an...

Embodiment 2

[0024] A method for cultivating pearls of Pinctada martensii microparticles for improving production, comprising the steps of:

[0025] (1) Preparation of nacre powder: select the brightly colored shells of Pinctada martensii nacre, remove the cuticle and prism layer of the shells, pulverize and sieve to obtain nacre powder with a particle size of 100 microns;

[0026] (2) Preparation of mantle slices: select 1.5-year-old Pinctada martensii as donor shellfish to make mantle slices, cut mantle strips at 1 mm above and below the mantle color line, and cut the mantle strips into 1.0 Small pieces of tissue in mm;

[0027] (3) Cell suspension preparation: under the condition of 28°C, use trypsin at a concentration of 150 units / ml to digest the small pieces of tissue for 2 hours, centrifuge at 1500 rpm for 5 minutes, and retain the supernatant; centrifuge at 3000 rpm for 10 Minutes, use sterilized seawater to suspend the cells to obtain a cell suspension;

[0028] (4) Preparation ...

Embodiment 3

[0032] In December 2016, pearls of Pinctada martensii were produced in Zhanjiang Xuwen Yinhui Pearl Breeding Farmers Professional Cooperative. Choose the shell with bright nacre and no damage, polish and remove the cuticle and prism layer, crush the shell nacre, and sieve it to prepare nacre powder with a thickness of 50-100 microns. Select 500 healthy individuals from the cultured population to plant nuclei and raise pearls, and the shell height is >5.0cm. Select 10 healthy individuals from the 1.0-year-old breeding population as donor shellfish, make mantle pieces, cut out mantle strips along the color line, and use a scalpel to cut the mantle strips into 0.5mm×0.5mm tissue blocks ;At room temperature, use 100U / ml trypsin solution to digest the tissue block for 2 hours; use a centrifuge to centrifuge at a speed of 1500 rpm for 5 minutes, and transfer the supernatant to another centrifuge tube for the second time Centrifuge at a speed of 3000 rpm for 10 minutes, suspend cell...

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PUM

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Abstract

The invention discloses a cultivation method for increasing the yield of pinctada martensii pearl particles. The method comprises the following steps: preparing a 50-100 microns pearl layer powder byutilizing shell nacre; selecting 1.0-1.5 age individuals as donor shellfish for preparing a mantle minor septum; carrying out enzymolysis on the mantle minor septum by utilizing trypsin to obtain cytochylema; adding the pearl layer powder into the cytochylema to prepare a mixed liquid and injecting the mixed liquid into connective tissues near the top of receptor shell through a mantle cavity; after 6 months of cultivation, carrying out second-time injection; then carrying out 12 months of cultivation and harvesting pearls. By adopting the method, the yield of the pearl particles can be increased, and good economic and social benefits are produced.

Description

technical field [0001] The invention relates to a method for cultivating pearls of Pinctada martensii, in particular to a method for cultivating pearls of Pinctada martensii for increasing the yield of microparticle pearls, and belongs to the technical field of cultivation of Pinctada martensii. Background technique [0002] The shellfish used to produce seawater pearls in my country include Pinctada martensii, Pinctada maxima, Pinctada oyster and Penguin pearl oyster. At present, Pinctada martensii is one of the main shellfish producing seawater pearls in my country. It is used to produce small and medium-sized nucleated pearls of 6.0-8.0mm. It reached the peak of the industry in the 1990s, and the annual pearl production reached about 20 tons. Nucleated pearl production is a complex process that requires implantation surgery. The basic process of implanting nucleus operation is to transplant the small piece of mantle (donor shellfish) and bead nucleus into the visceral co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/56A01K61/57
CPCA01K61/56A01K61/57Y02A40/81
Inventor 邓岳文王庆恒梁飞龙李俊辉郑哲
Owner GUANGDONG OCEAN UNIVERSITY
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