Method for preparing pinctada martensii high F value oligopeptide by enzymatic method

Abstract

The invention discloses a method for preparing pinctada martensii high F value oligopeptide by an enzymatic method, relates to enzymatic hydrolysis of a pinctada martensii meat protein and desorption of an aromatic amino acid in a hydrolysate by adopting an effective separation means, and provides a method for preparing an oligopeptide mixture with the molecular weight of less than 2,000 Da and the F value (the molar ratio of a branched chain amino acid to the aromatic amino acid) of more than 20 by adopting the enzymatic hydrolysis of the pinctada martensii meat protein and an adsorbent adsorption and separation desorption effect. In the method, an aquatic animal protein is first taken as a raw material to prepare the high F value oligopeptide; the raw material is extracted by using hot water in a preparation process, the extract can be used for extracting taurine, and the precipitated protein is taken as an enzymatic substrate, so that the resource is utilized fully and effectively; a single enzyme, namely pancreatin is used as the preferred active enzyme; and the aromatic amino acid is desorbed by adopting a method of combining activated carbon static adsorption and gel filtration chromatography to prepare a high F value oligopeptide product.

Description

technical field [0001] The invention belongs to an enzymatic method for preparing high F-value oligopeptides from Pinctada martensii. Background technique [0002] Active peptides are an important class of functional factors in functional health foods. Most of the active peptides currently on the market use more expensive protein sources such as soybean protein isolate and milk protein. Using these types of raw materials as substrate proteins results in high product costs and high sales prices. [0003] Pinctada martensii, also known as Hepu pearl oyster, is the main species of seawater pearl culture in southern my country. It is mainly distributed in the coastal areas of Guangdong, Guangxi, Hainan and Taiwan in my country, and also in the coastal waters of Japan and Villa. With the development of the pearl breeding industry, the seawater pearl cultivation area in Guangdong Province has exceeded 50,000 mu, with an annual output of more than 200,000 tons of raw pearls. Fo...

AI Technical Summary

Problems solved by technology

However, from the perspective of the development and utilization of protein raw materials, there are relatively few studies on the use of aquatic protein raw materials for the preparation of high F value oligopeptides, especially the research on shellfish protein is basically blank

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