Cultivating method of new breeding strain of pinctada martensii with high carotenoid content

Abstract

The invention discloses a cultivating method of a new breeding strain of pinctada martensii with high carotenoid content. The cultivating method comprises the basic steps of parent selection, population construction, larval cultivation, sea area breeding and multi-generation population selection, and the new breeding strain of pinctada martensii with the high carotenoid content can be cultivated; for the obtained new strain, prismjatic layers of 96% of individuals are golden yellow, pallia, adductor muscles and gill filaments of 96% of the individuals are golden yellow, and the carotenoid content in pinctada martensii is 2-5 times that of control population. The new strain of pinctada martensii with the high carotenoid content is cultivated through a population selection breeding technology, obvious golden yellow is achieved, and the carotenoid content in pinctada martensii is high; the method is easy to popularize in production units and has a significant practical value and economic value.

Description

technical field

[0001] The invention relates to a method for breeding fine varieties of Pinctada martensii, in particular to a method for cultivating new strains of Pinctada martensii with high content of carotenoids, and belongs to the technical field of aquaculture. Background technique

[0002] Carotenoids are a class of fat-soluble colored compounds that widely exist in nature and can only be synthesized in plants, fungi and some bacteria. Since Kuhn and Karrer first elucidated the structure of β-carotene in 1928, more than 800 carotenoids have been reported. Carotenoids have important biological functions in animals. The intake of carotenoids in the human body is closely related to the strength of antioxidant, cell regulation, gene expression and immunity. Carotenoids can also effectively reduce the risk of cancer and cardiovascular diseases. risk of other diseases. For aquatic animals, carotenoids can improve egg quality, reduce embryonic development mortality, impro...

Examples

Embodiment

[0022] Examples The following steps were used to implement the present invention: In April and June 2009, the population was constructed. Select the individual Pinctada martensii individuals whose shell prism layer, mantle, adductor muscle and gill filaments are all golden yellow as the parents. The shellfish is 2 years old and the shell length is 6.0-6.5cm. The gonads are cut and rinsed with a 300-mesh sieve. , Disperse gametes; fertilize the female parental eggs with the male sperm, and hatch the fertilized eggs in a 1000L incubator; 20 hours after fertilization, transfer the floating D-type larvae to a 1000L glass fiber reinforced plastic tank for cultivation; the hatching water temperature is 26-28 ℃, and the salinity is 28 ;The density of the larvae is controlled at 1 / ml, slightly inflated, and the water is not changed during the larvae cultivation; the yeast flakes are fed during the planktonic period, and the feeding amount is 0.1-0.5mg / l; After the larvae attach, chang...

technical field

[0001] The invention relates to a method for breeding fine varieties of Pinctada martensii, in particular to a method for cultivating new strains of Pinctada martensii with high content of carotenoids, and belongs to the technical field of aquaculture. Background technique

[0002] Carotenoids are a class of fat-soluble colored compounds that widely exist in nature and can only be synthesized in plants, fungi and some bacteria. Since Kuhn and Karrer first elucidated the structure of β-carotene in 1928, more than 800 carotenoids have been reported. Carotenoids have important biological functions in animals. The intake of carotenoids in the human body is closely related to the strength of antioxidant, cell regulation, gene expression and immunity. Carotenoids can also effectively reduce the risk of cancer and cardiovascular diseases. risk of other diseases. For aquatic animals, carotenoids can improve egg quality, reduce embryonic development mortality, impro...

Examples

Embodiment

[0022] Examples The following steps were used to implement the present invention: In April and June 2009, the population was constructed. Select the individual Pinctada martensii individuals whose shell prism layer, mantle, adductor muscle and gill filaments are all golden yellow as the parents. The shellfish is 2 years old and the shell length is 6.0-6.5cm. The gonads are cut and rinsed with a 300-mesh sieve. , Disperse gametes; fertilize the female parental eggs with the male sperm, and hatch the fertilized eggs in a 1000L incubator; 20 hours after fertilization, transfer the floating D-type larvae to a 1000L glass fiber reinforced plastic tank for cultivation; the hatching water temperature is 26-28 ℃, and the salinity is 28 ;The density of the larvae is controlled at 1 / ml, slightly inflated, and the water is not changed during the larvae cultivation; the yeast flakes are fed during the planktonic period, and the feeding amount is 0.1-0.5mg / l; After the larvae attach, chang...