Bacillus indius fermentation culture method

A technology for fermentation and cultivation of Bacillus indica, applied in the field of fermentation and cultivation of Bacillus indica, can solve the problems of unfavorable promotion and application of Bacillus indica, high cost, etc., and achieve the effects of cost reduction, low price and simple operation

Inactive Publication Date: 2014-10-15
DALIAN OCEAN UNIV +1
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  • Abstract
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Problems solved by technology

[0002] Bacillus indica was first discovered (2004) in a freshwater body in India. It was first discovered in seawater in 2009. In 2010, it was found that its cells contained carotenoids that cannot be destroyed by gastric juice, which can be safely used as food additives At present, the laboratory culture of Bacillus indica mainly utilizes beef extract peptone or 2216E medium for routine experiments. The cost of cultivating Bacillus indica in large quantities with this conventional medium is not conducive to the promotion and application of Bacillus indica

Method used

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Embodiment Construction

[0008] The specific implementation manner of the embodiment of the present invention will be described below. A method for fermenting and cultivating Bacillus indica, which is carried out according to the following steps: first, a culture medium is prepared, and the culture medium is a seawater solution based on seawater, wherein the content of soybean meal is 0.2-0.4g / 100ml, and the content of wheat bran is 0.2-0.4g / 100ml, the content of yeast extract is 0.1g / 100ml; after the configuration is completed, adjust the pH value of the medium to 7-7.5, and then inoculate the medium with Bacillus indica, the Bacillus indica used here is Bacillus indica L15, The strain has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee in 2013, and the preservation number is CGMCC No.1.12725. The concentration of inoculated Bacillus indica L15 was 10 8 cfu / ml, the inoculum amount is 1-2%, and the culture temperature is 25-30°C. Pu...

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Abstract

The invention discloses a bacillus indius fermentation culture method which is characterized by comprising the following steps: inoculation of bacillus indius into a medium with the pH of 7-7.5; wherein the inoculation size is 1-2%, the culture temperature is 25-30 DEG C, the liquid filling volume is 20-40% of a container, the table concentrator rotating speed is 150-170r / min, the culture time is 48 hours, and the medium is a sea water solution with the soybean meal content of 0.2-0.4g / 100mL, the wheat bran content of 0.2-0.4g / 100mL and the yeast extract content of 0.1g / 100ml. The bacillus indius fermentation culture method is simple, low in cost, and suitable for promotion and application.

Description

technical field [0001] The invention relates to a method for fermentation and cultivation of bacteria, in particular to a method for fermentation and cultivation of Bacillus indica. Background technique [0002] Bacillus indica was first discovered (2004) in a freshwater body in India. It was first discovered in seawater in 2009. In 2010, it was found that its cells contained carotenoids that cannot be destroyed by gastric juice, which can be safely used as food additives At present, the laboratory culture of Bacillus indica mainly utilizes beef extract peptone or 2216E medium for routine experiments. The cost of cultivating Bacillus indica in large quantities with this conventional medium is relatively high, which is not conducive to the promotion and application of Bacillus indica. Therefore, people in the industry now expect to find a low-cost method for cultivating Bacillus indica. Contents of the invention [0003] The present invention aims to solve the above-mentio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/07
Inventor 张东升李华刘洋宋坚王震毛东东
Owner DALIAN OCEAN UNIV
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