Antibacterial and antitumor orthopaedic implantation material and preparation method thereof

An implant material and anti-tumor technology, applied in medical science, prosthesis, etc., can solve the problems of local recurrence, long duration, and long time consumption, so as to prevent recurrence and metastasis, prevent adhesion and reproduction, and avoid the use of antibiotics Effect

Inactive Publication Date: 2013-12-25
GUANGZHOU GENERAL HOSPITAL OF GUANGZHOU MILITARY COMMAND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The traditional treatment of bone and soft tissue tumors is extensive resection of tumors including normal tissues, tumor inactivation and reimplantation, and various artificial prosthesis replacements such as endoluminal catheterization, joint replacement, and various biological bone implants. Limb salvage treatment by transplantation and other methods, and in the process of bone tumor surgery, due to the particularity of the tumor site, such as joints and spine, a large number of blood vessels and nerves are often adhered, which will take a long time during the entire operation and easily lead to infection
At present, in order to avoid the infection caused by the initial stage of implantation, the method of drug control and wound disinfection in the later stage is mostly used. This method is complicated and cumbersome, and lasts for a long time. After the tumor is removed, local recurrence and systemic metastasis often occur.
[0003] Commonly used orthopedic implant materials include pure titanium, titanium alloy, stainless steel and other metal materials, as well as degradable polylactic acid, autologous bone, allogeneic bone, and xenograft bone, etc., but the surface properties of the materials cannot meet the anti-tumor and antibacterial requirements.

Method used

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  • Antibacterial and antitumor orthopaedic implantation material and preparation method thereof
  • Antibacterial and antitumor orthopaedic implantation material and preparation method thereof
  • Antibacterial and antitumor orthopaedic implantation material and preparation method thereof

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preparation example Construction

[0029] A preparation method for an antibacterial and antitumor orthopedic implant material, comprising the following steps:

[0030] 1) After the surface of the medical pure titanium sheet is polished with sandpaper, it is ultrasonically washed with ethanol and acetone for 25-35 minutes respectively, and then washed with HNO 3 Polish with HF mixture for 25-35 minutes, wash and dry;

[0031] 2) React the polished pure titanium with dopamine solution at 20-30°C for 10-14 hours to bind dopamine to the surface of the pure titanium sheet, then wash the dopamine-bound pure titanium sheet with PBS buffer solution, and blow it with liquid nitrogen. Dry, repeat this process 2 to 4 times, the obtained material is marked as DM-Ti;

[0032] 3) Under ultrasonic conditions, add heparin sodium solution dropwise into an equal volume of polylysine solution to form a suspension of polylysine-heparin sodium particles, and then put DM-Ti into the suspension The solution was reacted for 10-14 ho...

Embodiment 1

[0043] 1) Take 1cm 2 The medical pure titanium sheet was polished with 120-grit sandpaper, then ultrasonically washed with ethanol for 30 minutes, then ultrasonically washed with acetone for 30 minutes, and then placed in a solution containing 12.5 mg / mL HNO 3 and 12.5 mg / mL HF mixture for 30 minutes, washed with deionized water, and dried;

[0044] 2) Prepare a 0.2 mg / mL dopamine solution with Tris-HCl with pH 8.5 as solvent, place the polished titanium in the dopamine solution, react at room temperature for 12 hours, wash it with PBS buffer solution, and blow it with liquid nitrogen. Dry, repeat this process 3 times, the obtained material is marked as DM-Ti;

[0045]3) Using PBS buffer as a solvent, prepare 5 mg / mL sodium heparin solution and 0.5 mg / mL polylysine solution respectively, and add the heparin sodium solution dropwise to an equal volume of polylysine under ultrasonic conditions After the acid solution, continue to sonicate for 30s to form a suspension of polyly...

Embodiment 2

[0048] 1) Take 1cm 2 The medical pure titanium sheet was polished with 120-grit sandpaper, then ultrasonically washed with ethanol for 25 minutes, then ultrasonically washed with acetone for 25 minutes, and then placed in a solution containing 11 mg / mL HNO 3 and 11 mg / mL HF mixture for 35 minutes, washed with deionized water, and dried;

[0049] 2) Prepare a 0.1 mg / mL dopamine solution with Tris-HCl with pH 8.7 as solvent, place the polished titanium sheet in the dopamine solution, react at room temperature for 14 hours, wash with PBS buffer, and liquid nitrogen Blow dry, repeat this process 2 times, the obtained material is marked as DM-Ti;

[0050] 3) Using PBS buffer as a solvent, prepare 6 mg / mL sodium heparin solution and 0.6 mg / mL polylysine solution respectively, and add the heparin sodium solution dropwise to an equal volume of polylysine under ultrasonic conditions After the acid solution, continue to sonicate for 30s to form a suspension of polylysine-heparin sodiu...

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Abstract

The invention discloses an antibacterial and antitumor orthopaedic implantation material and a preparation method thereof. The implantation material is a medical pure titanium sheet with the surface modified by chitosan, methotrexate, heparin sodium, polylysine and dopamine. According to the material, dopamine serves as a bridge to allow polylysine and a heparin sodium particle to be bonded on the surface of titanium; heparin, as a good anticoagulant, can improve biocompatibility between the material and blood, and is bonded with chitosan and methotrexate with antibacterial and antitumor effects through an electrostatic effect. Physiological-biochemical characteristics of drugs are utilized ingeniously to form an orderly and uniform antibacterial and antitumor coating with high biocompatibility on the surface of the titanium, and the prepared implantation material has good antibacterial and antitumor activity, and can effectively prevent bacterial adhesion and reproduction, as well as recurrence and transfer of a cancer.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to an orthopedic implant material and a preparation method thereof, in particular to an antibacterial and anti-tumor orthopedic implant material and a preparation method thereof. Background technique [0002] The traditional treatment of bone and soft tissue tumors is extensive resection of tumors including normal tissues, tumor inactivation and reimplantation, and various artificial prosthesis replacements such as endoluminal catheterization, joint replacement, and various biological bone implants. Limb salvage treatment by transplantation and other methods, and in the process of bone tumor surgery, due to the particularity of the tumor site, such as joints and spine, a large number of blood vessels and nerves are often adhered, which will take a long time during the entire operation and easily lead to infection . At present, in order to avoid the infection caused by the initial ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/06A61L27/20A61L27/54A61L27/22
Inventor 李丽华张余李梅何鹏夏虹尹庆水郑冠
Owner GUANGZHOU GENERAL HOSPITAL OF GUANGZHOU MILITARY COMMAND
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