76 results about "Cryptocaryon" patented technology

The invention relates to a method for preventing and treating a cryptocaryon irritans disease through a biological method. The method particularly comprises the step that biological prevention and treatment are conducted by removing cryptocaryon irritans miracidia by means of filter feeders. The method for conducting biological prevention and treatment on the cryptocaryon irritans disease by means of the filter feeders is high in operability, and no adverse effect can be generated to the environment; embodiment results show that the method achieves both a treatment effect and a prevention effect on the cryptocaryon irritans disease, and cured fishes cannot suffer from the cryptocaryon irritans disease in few months. The key of the method is selection of the filter feeders and determination of the putting quantity, and the prevention and treatment effects of the method can be enhanced by selecting the proper filter feeders and determining the appropriate putting quantity.

Disclosed is a discrimination method for disease degree of large yellow croakers infected with Cryptocaryon irritans, and the method relates to large yellow croakers. The method comprises firstly performing data preprocessing, building a mathematical method by using the 14 impact factors, and building a dimensionality reduction method. The method has features of being quantitative in discrimination, simple and convenient, and high in accuracy and reliability. Random sampling test shows that by use of the method, the severe degree of disease of large yellow croaker infected with Cryptocaryon irritans can be accurately determined by use of measured values of water environment factors, and the accuracy rate is up to more than 90%. The method can be applied to determine the sea area and severity of possible Cryptocaryon irritans infection, and used to direct the prevention and control of Cryptocaryon irritans infection in large yellow croakers. The method is suitable for discrimination on occurrence of Cryptocaryon irritans infection, and also provides an important reference for Cryptocaryon irritans infection in other marine fishes.

The invention relates to a production method for cryptocaryon irritant prevention trachinotus ovatus seedlings, and belongs to the field of disease resistance fry production, and specifically relates to the field of cryptocaryon irritant prevention fry production. A method of varying temperature infection and varying temperature culture is firstly used to produce disease resistance trachinotus ovatus seedlings. Water temperature in infection and cultivation water temperature in 10 days after infection keep certain temperature difference, so as to enhance immune effect of cryptocaryon irritant live larva, to further combine with rotational culture. The method obtains cryptocaryon irritant prevention trachinotus ovatus seedlings in a relatively long effective immune protection period. In the eighth month after immunity, the seedlings still have relative protection rate of 52%, and in the ninth month, the seedlings still have relative protection rate of 28%.

The invention relates to a production method for cryptocaryon irritant prevention epinephelus coioides seedlings, and belongs to the field of disease resistance fry production, and specifically relates to the field of cryptocaryon irritant prevention fry production. A method of varying temperature infection and varying temperature culture is firstly used to produce disease resistance epinephelus coioides seedlings. Water temperature in infection and cultivation water temperature in 10 days after infection keep certain temperature difference, so as to enhance immune effect of cryptocaryon irritant live larva, to further combine with rotational culture. The method obtains cryptocaryon irritant prevention epinephelus coioides seedlings in a relatively long effective immune protection period. In the eighth month after immunity, the seedlings still have relative protection rate of 60%, and in the ninth month, the seedlings still have relative protection rate of 34%.

The invention relates to a preparation method for subunit vaccines of Cryptocaryon irritans and application of the subunit vaccines of the Cryptocaryon irritans. Approval application and industrialization of the whole organism vaccines of the Cryptocaryon irritans encounter a great challenge, mostly because the parasites cannot be cultured in vitro and only breed on fish bodies, enough Cryptocaryon irritans bodies for producing the vaccines are difficult to obtain, most importantly, the normative production process and the quality standard are difficult to make, and thus, people are forced to conduct research in a deeper level to develop genetic engineering vaccines. Candidate genes of surface proteins C1 are screened from a transcriptome database of the Cryptocaryon irritans, and recombination proteins are expressed on tetrahymena, and accordingly the vaccines are prepared for immunizing seawater fishes; and by detecting the immunogenicity and protectiveness of the vaccines, effective protective antigenic genes of the Cryptocaryon irritans are screened.

The invention discloses a prevention and control method for cryptocaryon irritans of scatophagus argus. According to physiological and ecological features of the scatophagus argus, the purpose of preventing and controlling the cryptocaryon irritans of the scatophagus argus is met with a combined method of nutrient enrichment cultivation and disease prevention of the scatophagus argus, drug therapy of the cryptocaryon irritans and ecological regulation, wherein therapeutic drugs are copper acetate and zeolite powder combined with copper acetate, furacilin antibiotics or an algae-inhibitory compound and furacilin antibiotics combined with the algae-inhibitory compound. The survival rate of the young scatophagus argus intensively cultivated with the method is greatly increased and reaches more than 95%; the fry survival rate of the diseased scatophagus argus is increased from 0% to 64-88%, so that the economical loss is greatly reduced. The method is remarkable and stable in effect and easy to master, popularize and apply, has the characteristics of economy, good actual effect, low toxicity, easiness for operation and the like, and has an important actual effect for establishing a technical system for disease prevention and control of the scatophagus argus and promoting the development of the breeding industry of the scatophagus argus.

The invention discloses a cyst removal method for controlling fish cryptocaryon irritans. A cloth liner with the strong attaching effect on cryptocaryon irritans brown cysts is screened out mainly and laid on a water bottom of fish infected with the cryptocaryon irritans, the cryptocaryon irritans brown is nourished, falls down and is attached to the cloth liner, the cryptocaryon irritans brown cysts are removed by removing the cloth liner, the life history of the cryptocaryon irritans brown is cut off, and deaths caused when fish is repeatedly infected with the cryptocaryon irritans brown are reduced. By screening the materials, the cloth liner which is resistant to corrosion and free of toxicity and has the strong attaching effect on the cryptocaryon irritans brown cysts is obtained, the method for removing the cryptocaryon irritans brown cysts is used for treating plectorhinchus cinctus infected with cryptocaryon irritans, and the relative protection rate can reach 98%.

The present invention discloses a cyst inactivation method for controlling the cryptocaryon irritans disease of fishes. Mainly through screening a cloth liner having a strong adhesion effect on cryptocaryon irritans cysts, laying the cloth liner to the water bottom of the fishes infected by the cryptocaryon irritans disease so as to enable trophozoites of the cryptocaryon irritans to be adhered on the cloth liner after falling off, and removing the cloth liner to remove the cryptocaryon irritans cysts, life history of the cryptocaryon irritans is cut off, and death of the fishes caused by repeated infection of the cryptocaryon irritans is reduced. According to the method, the cloth liner which is corrosion resisting and nontoxic and has a strong adhesion effect on the cysts is obtained through screening materials, plectorhinchus cinctus infected by the cryptocaryon irritans disease is treated by using the method of removing the cryptocaryon irritans cysts, and relative protection ratio can reach 98%. Drying treatment and heat treatment are carried out on the cysts, and the results show that the cysts can be 100% inactivated when the cysts are dried for 120 min, and the cysts can be 100% inactivated when the cysts are processed for 3 min at 43 DEG C or processed for 1 min at 48 DEG C.

The invention discloses a gene complete sequence of siganus oramin L-amino acid oxidase and application thereof. The gene complete sequence of the siganus oramin L-amino acid oxidase is 1725bp, the length of an open reading frame is 1584bp, and the gene complete sequence is specifically in a 43-1626 region and is used for coding 527 amino acids. The siganus oramin L-amino acid oxidase is obtained from siganus oramin serum through separation and purification, and has a function of killing cryptocaryon irritans brown and a bactericidal activity on typical gram-positive bacteria (staphylococcus aureus) and gram-negative bacteria (escherichia coli). In an optimized high-definition enzyme PCR (Polymerase Chain Reaction) system, by using total siganus oramin spleen cDNA as a template and adopting a pair of designed specific primers, the gene sequence coding the protein can be rapidly accurately cloned and used for the subsequent genetic engineering.

The invention discloses a physical prevention and treatment device for a coptocaryon irritans disease and an application method thereof. The physical prevention and treatment device is a metal net made of a copper alloy material and is laid at the bottom of a culture pond. A corresponding area of metal net made of the copper alloy material is used according to the area of the bottom of the culturepond. During an epidemic season of the coptocaryon irritans disease, the metal net made of the copper alloy material is laid at the bottom of the culture pond in a fixed time period before an outbreak of the coptocaryon irritans disease, and is taken out after a period of time, and the metal net is operated every other day for 8 days in all; and the metal net does not need to be used after 8 days, so that cryptocaryon irritans cysts can be basically killed and the outbreak of the coptocaryon irritans disease is avoided. The physical prevention and treatment device is high in operability, lowin cost, safe and nontoxic, can be widely applied to marine aquaculture, and is a novel physical prevention and treatment method for effectively inhibiting mass outbreak of the coptocaryon irritans disease.

The invention discloses an oil-adjuvant vaccine of larvae membrane protein of cryptocaryon irritans, and a preparation method and applications thereof. The preparation method of the vaccine comprises the following steps: (1) collecting live larvae of the cryptocaryon irritans; (2) extracting the larvae membrane protein of the cryptocaryon irritans; and (3) adding PBS in the larvae membrane protein as water phase and mixing No.10 white oil, Span-80 and aluminum stearate as oil phase, emulsifying the larvae membrane protein in the volume ratio of the water phase to the oil phase, which is 3:7, thus obtaining the oil-adjuvant vaccine of the larvae membrane protein of the cryptocaryon irritans. The preparation method of the oil-adjuvant vaccine of the larvae membrane protein of the cryptocaryon irritant is simple and practicable, and the obtained oil-adjuvant vaccine of the larvae membrane protein of the cryptocaryon irritans has high safety, obvious immunoprotection effect and no toxicity to fish, can be used for preventing and treating the parasitic infection of sea fish and can absolutely meet the demand of actual production.

Provided are a method and device for killing cryptocaryon irritans cysts through hydroxyl radicals. The method is provided with a water storage tank, a couture pond, a high-frequency high-voltage power source, a modular oxygen activity fragment generator, an online oxygen activity fragment detector, a Venturi gas-liquid mixing and dissolving device, a liquid-liquid mixing and dissolving device, a TRO detector, a motor, an automatic rotary brush, a water pump, a valve, a gas pipeline and a liquid main pipeline. An air inlet of the modular oxygen activity fragment generator is externally connected with an oxygen source, and an oxygen activity fragment air outlet of the modular oxygen activity fragment generator is connected with the online oxygen activity fragment detector, and an air suction opening of the Venturi gas-liquid mixing and dissolving device in sequence. A water outlet of the water storage tank is connected with a water inlet of the water pump, a water outlet of the water pump is connected with a liquid inlet of the Venturi gas-liquid mixing and dissolving device, a liquid outlet of the Venturi gas-liquid mixing and dissolving device is connected with a liquid inlet of the liquid-liquid mixing and dissolving device, and a liquid outlet of the liquid-liquid mixing and dissolving device is connected with the TRO detector and a water inlet of the culture pond in sequence. The automatic rotary brush controlled by the motor is arranged in the middle of the culture pond, and a water outlet in the bottom of the culture pond is externally connected with sea water.

The invention relates to a compound Chinese herbal medicine for treating a marine fish cryptocaryon irritans disease. The compound Chinese herbal medicine is characterized by being prepared from, by weight, 35-45 parts of stone-like omphalia, 25-35 parts of fructus quisqualis, 15-25 parts of cortex meliae, 15-25 parts of fructus cnidii, 15-25 parts of semen torreyae, 25-35 parts of Japanese flowering fern rhizomes, 25-35 parts of radix stemonae, 25-35 parts of pericarpium granati and 5-10 parts of cortex magnoliae officinalis. According to the traditional Chinese medicine composition for treating the marine fish cryptocaryon irritans disease, in the formula, stone-like omphalia, semen torreyae, fructus quisqualis, cortex meliae, fructus cnidii, Japanese flowering fern rhizomes, radix stemonae and radix stemonae for disinfestation serve as monarch drugs, cortex magnoliae officinalis serves as an assistant drug to remove dampness and disperse fullness, fructus aurantii immaturus regulates the intestines and stomach to disperse accumulation and stagnation, all materials are combined for use, the effects of disinfestation, dampness removing, fullness dispersing, accumulation dispersing and the like are achieved, and the compound Chinese herbal medicine is good in curative effect, free of public hazard and residue, convenient to use and especially suitable for factory marine fish breeding.

The invention relates to a direct fluorescence labeling method for displaying a cryptocaryon irritans brown microtubule cytoskeleton structure, which comprises the following steps of: preparing a glass slide coated with polylysine; permeating cryptocaryon irritans brown cells through solution PHEM (Postherpetic Erythema Multiforme) containing saponin, and adding PHEM solution for immersion bath; fixing permeated cells through paraformaldehyde solution, and carrying out immersion bath through the PHEM solution, and transferring the fixed cells to the glass slide coated with the polylysine; dripping Triton X-100 water solution on the cells after removing extra fluid, carrying out immersion bath through the PHEM solution, and rinsing through 0.01mol / L of phosphate buffer; rinsing through 0.10mol / L of PBS (Phosphate Buffer Solution) after keeping from light, hatching through the PBS containing 1mu mol / L of fluorescence taxoid at room temperature, removing extra solution, and capping the glass slide; and finally, observing through a microscope and taking photos. The method is simple to operate, has high efficiency, is environmental-friendly and has no pollution.

The invention relates to a composite preparation used for treating larimichthys crocea cryptocaryon irritans disease and application thereof. The composite preparation comprises, 10 wt%-15 wt% of nitazoxanide, 5 wt%-7 wt% of copper sulfate, 25 wt%-30 wt% of terramycin, and 48 wt%-60 wt% of a solvent, and the sum of the above raw materials is 100%. The usage method comprises after larimichthys crocea is definitely diagnosed to be attacked by cryptocaryon irritans disease, splashing the above preparation according to the amount of 8-10 g / m<3>, continuously performing dipping bath for 3 days, so as to effectively cure larimichthys crocea cryptocaryon irritans disease and prevent bacterial secondary infection. The preparation is simple in preparation method and convenient to use, and is capable of substantially reducing mortality of larimichthys crocea attacked by cryptocaryon irritans disease and raising culture economic benefit.

The invention discloses a breeding method of pseudosciaena crocea resisting cryptocaryon irritans based on whole genome selection, and belongs to the field of animal disease resistance breeding. The method comprises the steps of establishing a reference group; performing resistance trait measurement on the reference group; establishing a breeding group; carrying out genotyping on the reference group and the breeding group; establishing an optimal whole genome selection model; estimating a genomic breeding value GEBV of the breeding group; selecting individuals, with the GEBV ranked in the front, of the breeding group as parent fishes according to a certain selection intensity, and propagating to generate a first filial generation resisting the cryptocaryon irritans; and conducting cryptocaryon irritans toxicity attack verification on the first filial generation with resistance. The insect resistance is obviously improved; and the estimation accuracy of the genomic breeding value of thebreeding group is improved, the breeding period is greatly shortened, high-resistance offspring can be obtained within one generation, the economic loss of the cryptocaryon irritans to the pseudosciaena crocea breeding industry is reduced, reference and basis are provided for disease-resistant breeding of other fishes, and the method has a wide application prospect.

The invention discloses the application of tannic acid in killing and preventing ectoparasites of aquatic animals, and further, the application of tannic acid in killing and preventing external freshwater melon worms and seawater-stimulated Cryptocaryon of aquatic animals. Low toxicity, non-toxic to aquatic animals, less residue.

The invention discloses a method for controlling ichthyophthirius during trachinotus-ovatus cage culturing. The method includes the steps of observing and preventing, making of a netting device for controlling the ichthyophthirius in the mode that the trachinotus ovatus is soaked with fresh water, fresh water soaking, medicine treating, physical enhancing and the like. According to the method, the trachinotus ovatus is soaked with fresh water, cryptocaryon irritans loses the adsorption capacity in fresh water through sudden changing of osmotic pressure and is separated from a fish body, and if soaking time is long, polypide cells can be ruptured and died; after the ichthyophthirius is infected to the fish body and developed and matured, the ichthyophthirius is separated from a host after 3 days to 5 days, a cyst is formed and is subjected to asexual reproduction to be split into a plurality of larvae capable of infecting the fish body; if a cage is moved to a deep sea area with the good water quality and the high flowing speed, the cyst can be separated from the host to be taken away by water flow, and the controlling effect is better achieved. By means of the method, it can be achieved that the trachinotus ovatus is soaked with fresh water in a sea cage for controlling the cryptocaryon irritans; meanwhile, trachinotus ovatus cannot be directly caught in the whole course, and stress responses caused by catching are reduced.

The invention provides a Larimichthys crocea antibacterial peptide piscidin 5 like, a preparation method and applications thereof, relates to an antibacterial peptide, and discloses a Larimichthys crocea antibacterial peptide piscidin 5 like gene coding sequence, a Larimichthys crocea antibacterial peptide piscidin 5 like amino acid sequence, a Larimichthys crocea antibacterial peptide piscidin 5like preparation method, and applications of the Larimichthys crocea antibacterial peptide piscidin 5 like. The preparation method comprises: constructing a Lc-p5L recombinant expression vector; transforming the recombinant expression vector into host cells, and carrying out induced expression on the host cells to obtain an expression product; and separating and purifying the obtained expression product to obtain a recombinant protein rLc-p5L, wherein the recombinant protein rLc-p5L has inhibition and killing activity on the larvae and the trophozoite of Cryptocaryon irritans, can be used in the preparation of insect-resistant drugs, and can further be used as feed additives for preventing and controlling diseases in aquaculture.

The invention belongs to the technical field of chemical product uses, and discloses a prevention and control agent for killing ichthyophthirius multifiliis and cryptocaryon irritans outside aquatic animal bodies, wherein the prevention and control agent comprises, by weight, 5-25% of natamycin, 5-10% of a surfactant, 10-20% of a dissolution agent, and the balance of a solvent. According to the present invention, the natamycin is used, such that advantages of low toxicity, no toxicity on aquatic animals, and less residue are provided; the specific dissolution agent and the specific solvent are utilized, such that the dissolving effect is good, and the dissolution agent can help the entering of the prevention and control agent into the body so as to provide the significant ichthyophthirius multifiliis killing effect; and the prevention and control agent has characteristics of simple preparation, easy use, and low production cost.

The invention discloses a cryptocaryon irritans Brown prevention large yellow croaker fry production method, and belongs to the field of disease-resistance fry production, specifically the field of cryptocaryon irritans Brown fry production. The method comprises the steps: firstly producing disease-resistance large yellow croaker fries in modes of variable temperature infection and variable temperature cultivation; secondly maintaining a certain temperature difference between the water temperature during infection and cultivation water temperature in ten days, thereby improving the immune effect cryptocaryon irritans Brown larvae, so as to combine with alternate cultivation, and to obtain the cryptocaryon irritans Brown prevention large yellow croaker fry with a longer effective immune protection period, wherein the large yellow croaker fry still has a 62% relative protection rate in an eighth month, and still has a 30% relative protection rate in a ninth month.

The invention discloses a preparation method of surface-protein-C1 polyclonal antibody of cryptocaryon irritans brown and an application of the surface-protein-C1 polyclonal antibody. It is discovered by us study that a surface protein C1 gene of cryptocaryon irritans brown is a potentially effective protective antigen gene; an expression vector of rDNA of tetrinophore is established, the surface protein C1 gene of the cryptocaryon irritans brown is subjected to vitro expression and separation and purification of recombinant protein, the surface-protein-C1 polyclonal antibody is finally prepared, and the primary application of the surface-protein-C1 polyclonal antibody in terms of immunohistochemistry is performed.

The invention discloses a method for low-temperature preservation of trophonts and cysts of Cryptocaryon irritans, comprising the following steps: firstly, the trophonts and the cysts of the Cryptocaryon irritans are collected and preserved in a manual climatic cabinet at the temperature of 8 to 15 DEG C with a relative humidity of 90 percent according to 12 hour-light: 12 hour-dark cycle; sterilizing seawater is exchanged once at intervals of 5 days and the water exchange quantity is 20 percent; secondly, the trophonts and the cysts of the Cryptocaryon irritans which are preserved at low temperature are detected once every month, and the hatchability of the trophonts and the cysts and the infectivity of hatched larvaes when the temperature is returned to 27.0 DEG C are measured. By adoption of the method for preservation of the trophonts and the cysts of the Cryptocaryon irritans, the trophonts which are preserved for 4 months and the cysts which are preserved for 3 months can still hatch out larvaes with infectivity; the larvaes can propagate a great amount of offsprings under proper conditions, and the virulence of the offsprings is not reduced.

The invention adopts a method of cryptocaryon irritans locally-infected epinephelus coioides one-sided skin for immunity for the first time, adopts an optimized infection concentration and infection duration time to construct an immune model of the cryptocaryon irritans locally-infected epinephelus coioides one-sided skin for the first time, thoroughly eliminates an adverse effect brought by ''congenital differences'' in the process of local skin mucosal immune response in fish, and provides an ideal immune model for researching a local mucosal immune response law. The construction method forthe immune model mainly comprises the following five steps: (1) collecting live larvae of the cryptocaryon irritans and calculating a concentration; (2) grouping the epinephelus coioides and performing domestication; and (3) performing local skin immunization; (4) measuring infection intensity; and (5) performing treatment on locally-infected fish bodies.

The invention provides a method for biologically preventing and controlling cryptocaryon irritans infection by using tilapia mossambica. The method includes the steps of S1, performing seawater domestication on the tilapia mossambica; and S2, carrying out polyculture on the tilapia mossambica subjected to seawater domestication and fishes susceptible to cryptocaryon irritans. According to the method for biologically preventing and controlling the cryptocaryon irritans infection by using the tilapia mossambica, the red tilapia mossambica and trachinotus ovatus are subjected to polyculture, the polyculture ratio is 3:20, and the number of the tilapia mossambica is 19 / m<2>, so that the insect carrying amount of the trachinotus ovatus can be remarkably reduced, and the survival rate of the trachinotus ovatus can be increased; and the method can effectively prevent and control the secondary repeated infection of the cryptocaryon irritans to the fishes susceptible to the cryptocaryon irritans, can achieve the dual effects of preventing and controlling the cryptocaryon irritans, is an environment-friendly, green and safe biological control method, and is worthy of being popularized in marine fish culture.

The invention belongs to the field of biological prevention and control, and relates to application of honokiol in preparation of medicines for inhibiting and killing in vitro parasitic ciliates of fish. The invention discloses activity of the honokiol in inhibition of the in vitro parasitic ciliates of the fish, and applies the honokiol to prepare the medicinesthe application of the honokiol in preparation of the medicines for inhibiting and killing the in vitro parasitic ciliates of the fish; the honokiol is especiallyused for inhibiting and killing cryptocaryon irritans brown, the honokiolcan completely inhibit and kill parasites within an effective dose range, and achieves the effect of preventing and controlling seawater ichthyophthiriasis; the honokiol is a natural compound, is little in toxic and side effects and easy to degrade and remove, can avoid the problems such as environmental pollution and drug residue which are caused by abuse of chemicals, and is beneficial to promotepromotion of the sound development of aquaculture industry.

The invention discloses a compound Chinese herbal medicine preparation for preventing and treating cryptocaryon irritans in fishes. The compound Chinese herbal medicine preparation is characterized by consisting of the following components in percentage by weight: 38-42% of semen arecae, 18-22% of pericarpium granati, 18-22% of szechwan Chinaberry fruit, 8-12% of dark plum and 8-12% of radix stemonae. The compound Chinese herbal medicine preparation disclosed by the invention, on preventing and treating the cryptocaryon irritans in fishes, has the characteristics of being non-toxic, free from residue, free from environmental pollution, free from influence on the normal physiologies of the fishes. The adopted raw materials are low in cost, and a production process is simple.

The invention belongs to the field of fish disease prevention and control and in particular relates to a method for controlling cryptocaryon irritans diseases of fishes by using ozone. According to the method, by virtue of the characteristic that larvae of cryptocaryon irritans are centrally incubated at night, ozone is introduced into a breeding water body in a larva incubation period, and the ozone concentration is kept within a lowest concentration range which is capable of effectively deactivating the larvae of the cryptocaryon irritans; and by cutting off the life history of the cryptocaryon irritans, the purposes of treating and preventing the cryptocaryon irritans diseases can be achieved. By adopting the method, experiments that the larvae of the cryptocaryon irritans are treated with seawater of different ozone concentrations show that the lowest effective deactivation concentration is 0.1-0.15mg / L; and trachinotus ovatus is adopted for effect verification experiments, resultsshow that 411.4+14.7 cysts are generated by each fish on average when the ozone concentration is 0mg / L, 0.4+ / -0.1 cyst is generated by each fish on average when the ozone concentration is 0.1mg / L, and 0 cyst is generated by each fish on average when the ozone concentration is 0.15mg / L. Results show that when the ozone concentration is 0.1-0.15mg / L, the larva infection capability of the cryptocaryon irritans can be remarkably reduced (P is less than 0.05), and the cryptocaryon irritans diseases can be effectively controlled.

The invention discloses an exosome extraction and separation method of seawater parasitic ciliate cryptocaryon irritans, which comprises the following steps of: 1, collecting cysts of cryptocaryon irritans, cleaning the cysts, and incubating the cysts in sterile seawater containing antibiotics; 2, collecting the larvae in the infection period by using a sterile filter screen, and incubating and culturing the larvae in a culture medium containing antibiotics and exosome-free fetal calf serum; 3, centrifuging to remove larvae in an infection period, and collecting supernate; 4, sterilizing the supernate through a filter membrane, and concentrating the filtrate; 5, centrifuging the concentrated solution and collecting exosome coarse precipitate; 6, resuspending the exosome coarse precipitate by using sterile precooled PBS, and adding a protease inhibitor; and 7, centrifuging to remove supernate, and carrying out resuspension precipitation to obtain the exosome. According to the method, the exosome of cryptocaryon irritans can be effectively separated and extracted, the extracted exosome has a typical dish-shaped or saucer-shaped vesicle-shaped structure, the overall particle size meets the standard, and the extraction concentration of the exosome is high.