Preparation method for subunit vaccines of Cryptocaryon irritans and application of subunit vaccines of Cryptocaryon irritans

Abstract

The invention relates to a preparation method for subunit vaccines of Cryptocaryon irritans and application of the subunit vaccines of the Cryptocaryon irritans. Approval application and industrialization of the whole organism vaccines of the Cryptocaryon irritans encounter a great challenge, mostly because the parasites cannot be cultured in vitro and only breed on fish bodies, enough Cryptocaryon irritans bodies for producing the vaccines are difficult to obtain, most importantly, the normative production process and the quality standard are difficult to make, and thus, people are forced to conduct research in a deeper level to develop genetic engineering vaccines. Candidate genes of surface proteins C1 are screened from a transcriptome database of the Cryptocaryon irritans, and recombination proteins are expressed on tetrahymena, and accordingly the vaccines are prepared for immunizing seawater fishes; and by detecting the immunogenicity and protectiveness of the vaccines, effective protective antigenic genes of the Cryptocaryon irritans are screened.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a preparation method and an application of a cryptocaryon stimulating subunit vaccine. Background technique [0002] Cryptocaryon irritans is a ciliate that parasitizes marine fish and causes a serious fatal disease of marine fish—white spot disease. In recent years, due to the overloaded development of marine aquaculture in my country and the deterioration of the aquaculture environment, Cryptocaryoniasis has become a large-scale, frequent outbreak of severe infectious disease, causing very serious harm to seawater fishery. Due to the open nature of mariculture, it is difficult to eliminate the infection of Cryptocaryon irritant to fish, and there is no effective treatment drug and treatment method, which makes the prevention and treatment of this disease extremely difficult. Mainly adopt drug control and physical control (Colorni and Burgess, 1997; Cai Zepin...

AI Technical Summary

Problems solved by technology

However, the approval application and industrialization of the whole worm vaccine have encountered huge challenges, mainly because the parasite cannot be cultured in vitro, and must be reproduced on fish, so it is difficult to obtain enough worms to produce the vaccine. The most important thing is that it is difficult to formulate standardized production processes and quality standards, which forces us to conduct research at a deeper level and develop genetically engineered vaccines

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