23 results about "White spot disease" patented technology

The invention belongs to the technical field of biology, and particularly relates to application of hepcidin expression quantity in larimichthys crocea liver as larimichthys crocea visceral white-spot disease resistance evaluation index and a construction method. The hepcidin expression quantity in the larimichthys crocea liver is applied as the larimichthys crocea visceral white-spot disease resistance evaluation index. The construction method of the resistance evaluation index comprises the following steps: taking healthy small yellow croaker intraperitoneal injection pseudomonas proteinus strain bacterial liquid, and determining a half lethal dose in 96 hours; the method comprises the following steps: injecting a half lethal dose of bacterial liquid of pseudomonas proteinus strains into the abdominal cavity of healthy small yellow croaker for 96 hours, and after infection, taking different tissues to carry out hepcidin gene expression fluorescent quantitative PCR analysis; a regression model of gene expression quantity and bacterium loading quantity is established through fluorescence quantification, and the relationship between hepcidin expression quantity and disease resistance is constructed. Accurate disease-resistant phenotype information is provided for development of breeding of visceral white-spot disease-resistant improved varieties of the small yellow croakers, the accuracy of disease-resistant breeding is promoted, early detection of diseases is realized, and prevention and treatment of the diseases are effectively guided.

The application discloses a method for quantitatively analyzing the infection degree of white spots in the spleen of Pseudosciaena crocea based on machine learning, and relates to a threshold segmentation technology of machine learning. 1) Data acquisition: dissect the Pseudosciaena crocea infected with internal visceral white spot disease, use a high-definition camera to shoot the spleen, and acquire pictures of the white spots in the spleen to be quantitatively analyzed; 2) creation of an interactive segmentation GUI interface: used for reading the pictures of the white spots in the spleen of the Pseudosciaena crocea and automatically converting the pictures into binary images; 3) running of a main program: the main program is written by a python code and is used for the GUI interface to display the selected pictures on the interactive segmentation GUI interface, so that the pictures are provided for contrast segmentation in the next step; 4) picture processing: selecting the pictures, drawing a frame to select a region to be segmented, adjusting a segmentation threshold to acquire a relatively accurate segmentation result, and realizing visual interactive segmentation of the white spots in the spleen; and 5) quantitative analysis: realizing the quantitative analysis of the infection degree of the white spots in the spleen of the Pseudosciaena crocea by the proportion of the white spots in the spleen.

The invention relates to a Pseudomonas plecoglossicida pldB gene deletion mutant strain and a preparation method and application thereof, and belongs to the technical field of microorganisms and the field of algae preservation, the pldB gene of the mutant strain is deleted or inactivated, the nucleotide sequence of the pldB gene is shown as SEQ ID NO: 1, and the GenBank accession number of the Pseudomonas plecoglossicida is CP031146.1. The virulence of the mutant strain is obviously reduced compared with that of a wild strain of pseudomonas plecoglossicida; the strain can induce appropriate immune response of a host and up-regulate expression of anti-inflammatory factors, is beneficial to relieving immune pathological injury, has good attenuated live vaccine potential, and provides a brand new vaccine candidate strain for prevention and control of the visceral white-spot disease of the large yellow croaker.

This invention belongs to the field of genetic breeding technology for edible and medicinal fungi, and relates to a Cordyceps militaris strain with enhanced resistance to white spot disease and its creation method. This invention provides a safe overexpression site CmSH1 (SEQ ID NO:1) in the Cordyceps militaris genome, the gene Cmhyd1 (SEQ ID NO:2) encoding a hydrophobic protein in Cordyceps militaris, a Cordyceps militaris strain with enhanced resistance to white spot disease, a method for creating the Cordyceps militaris strain with enhanced resistance to white spot disease using CRISPR / Cas9 gene editing technology, and a corresponding vector. The Cordyceps militaris strain of this invention can enhance resistance to white spot disease, and the fruiting body develops normally. Its creation method avoids food safety issues caused by the integration of transgenic elements into the genome.

The invention provides application of a Zm00001eb155730 gene in corn breeding, and relates to the technical field of agricultural biology. The Zm00001eb155730 gene is applied to regulation and control of corn leukoderma resistance molecular marker assisted breeding. According to the invention, GWAS (Genome-Wide Association Study) analysis and QTL (Quantitative Trait Loci) analysis of a genetic linkage map are jointly positioned to S3-210892574 which is positioned in a chromosome 3 and is remarkably related to the resistance of the corn white spot disease, so that the functional gene Zm00001eb155730 for regulating and controlling the resistance of the corn white spot disease is excavated. The gene Zm00001eb155730 has a predominant haplotype in GWAS, the leukoderma resistance of the haplotype is significantly higher than that of other haplotypes, and a theoretical basis is provided for molecular marker-assisted selection and regulation of the corn leukoderma resistance.

The invention belongs to the technical field of plant disease prevention and treatment, and relates to application of an alternaria alternate nucleoside diphosphate sugar isomerase gene in prevention and treatment of allium tuberosum leukoderma. The invention provides a dsRNA (double-stranded ribonucleic acid) preparation taking an alternaria alternate nucleoside diphosphate glycoisomerase gene as a target and application of the dsRNA preparation in prevention and control of allium tuberosum leukoplakia. Normal transcription expression of the alternaria alternate nucleoside diphosphate glycoisomerase gene is interfered by dsRNA, so that influence on mycelial growth, spore production rate and the like of alternaria alternate can be realized; and the alternaria alternata can be used for green prevention and control of the white spot disease of the Chinese chives, and is high in pertinence and good in prevention and control effect. Meanwhile, pollution of chemical agents to the environment can be reduced by using the dsRNA preparation, the safety is high, and the application range is wide.

The invention discloses an SNP chip special for the visceral white-spot disease resistance character of large yellow croaker and application of the SNP chip, the whole-genome SNP chip for the visceral white-spot disease resistance character of the large yellow croaker comprises a probe combination, and the probe combination is used for identifying the genotype of each SNP site in the SNP site combination; the large yellow croaker whole-genome SNP chip provided by the invention contains 44948 loci, 420 of the loci are key SNP loci for resisting visceral white-spot disease, the large yellow croaker whole-genome SNP chip can be applied to large yellow croaker disease-resistant character genome selective breeding, and by adopting the large yellow croaker whole-genome SNP chip disclosed by the invention, a large yellow croaker sample can be quickly subjected to genetic typing; the method is applied to selective breeding of a visceral white-spot disease resistant character genome, and is beneficial to shortening the breeding period of the large yellow croaker and accelerating the breeding process of a new variety of the large yellow croaker.

The present invention discloses Staphylococcus epidermidis 2251 and its applications. Staphylococcus epidermidis 2251 has a preservation number of CGMCC NO. 30987. The applications of Staphylococcus epidermidis 2251 in preparing a biocontrol preparation or feed for preventing and treating aquatic diseases; the application of Staphylococcus epidermidis 2251 in inhibiting the growth of Pseudomonas plecoglossicida, and a biocontrol preparation or feed for preventing and treating aquatic diseases containing Staphylococcus epidermidis 2251. A strain of Staphylococcus epidermidis isolated and cultured in the present invention has verified the disease resistance of the strain through in vitro and in vivo experiments, and preliminarily explored its disease resistance mechanism, providing a reference for the prevention and treatment research of the visceral white spot disease of small yellow croaker.

The application discloses a culture device for Mesocyclops leuckarti and application thereof, and belongs to the technical field of fish culture. The culture device for Mesocyclops leuckarti comprises a cylinder, a top cover and a bottom cover, the top cover is movably connected with the top of the cylinder, and the bottom cover is fixedly connected with the bottom of the cylinder; a collecting pipe is arranged in the cylinder, the upper portion of the collecting pipe is a silk screen, the lower portion of the collecting pipe is a closed pipe, and a first light source is arranged in the collecting pipe; a second light source and a third light source are arranged on the inner side of the cylinder, and a temperature regulator and an oxygenator are arranged on the lower portion of the inner side of the cylinder. The culture device can realize large-scale continuous high-density culture of Mesocyclops leuckarti, can be applied to preparation of a biological prevention and treatment device for fish white spot disease, can reduce the abundance of water body Paramoecium caudatum and thus reduce the probability of fish suffering from the white spot disease, can provide other food sources for the fish, is beneficial to growth and development of the fish, and enriches the aquatic food web.

This invention discloses the application of Terminalia chebula in the prevention and treatment of Ichthyophthirius multifiliis (white spot disease) in aquatic animals. Toxicity experiments on grass carp kidney cells (CIK) showed that the maximum non-toxic concentration (NDC) of the aqueous extract of Terminalia chebula against CIK cells was 500 μg / mL. The inhibition of Ichthyophthirius multifiliis predator survival by the aqueous extract was dose-dependent, and the predator mortality rate reached 100% at a concentration of 100 mg / L (μg / mL). Furthermore, the median lethal concentration (LD50) of the aqueous extract against Ichthyophthirius multifiliis predators was calculated to be 14.577 mg / L. This invention is the first to propose the application of Terminalia chebula in inhibiting the survival rate of Ichthyophthirius multifiliis predators in fish at a non-toxic concentration to cells, providing a novel drug for the prevention and treatment of Ichthyophthirius multifiliis infection in aquatic animals. Terminalia chebula is a natural medicinal plant, environmentally friendly, and has the advantages of reducing the risk of drug residues.

The invention discloses application of arachidonic acid in induction improvement of corn leukoderma resistance, the arachidonic acid is applied to prevention and treatment of corn leukoderma for the first time, and experiments show that the arachidonic acid can improve the photosynthetic effective leaf area, Fm content, Fv / Fm content, NPQ content, soluble sugar content and soluble protein content of plants; and / or increasing the activity of disease-resistant-related defensive substances; and / or catalyzing synthesis of lignin and phenolic substances; and / or the content of Rfd, MDA and reducing sugar of the plant is reduced to induce and improve the corn leukoderma resistance, so that a new direction and basis can be provided for preventing and treating the corn leukoderma by adopting natural compounds. It is found that the arachidonic acid does not strictly depend on the total spraying amount for inducing and improving the leukoderma resistance, but when the spraying amount is the same and the concentration of the arachidonic acid in the spraying liquid is 15 [mu] M, the arachidonic acid is optimal for inducing and improving the leukoderma resistance. And the application of arachidonic acid is beneficial to reducing the particle size of starch particles in corn kernels under the pressure of leukoderma.

The invention relates to a primer pair, a composition, a kit and a method for detecting pseudomonas plecoglossicida causing large yellow croaker visceral white-spot disease based on an RPA-CRISPR / Cas12a technology, the nucleotide sequences of upstream primers RPA-F are respectively as shown in SEQ ID NO.1-4, the nucleotide sequences of downstream primers RPA-R corresponding to the upstream primers RPA-F are respectively as shown in SEQ ID NO.5-8, and the composition comprises the primer pair, crRNA, Cas12a protein and a fluorescent reporter probe. Compared with the prior art, the invention establishes a P. plecoglossicida detection method which is simple and convenient to operate, short in detection time, good in specificity and high in sensitivity and only needs common constant-temperature equipment, and is suitable for on-site rapid detection in basic laboratories and large yellow croaker farms.

This invention belongs to the field of molecular genetic breeding technology, specifically relating to the molecular marker Zm00001eb181850 gene and its application related to maize white spot disease resistance. This invention provides a molecular marker related to maize white spot disease resistance, the gene sequence of which is shown in SEQ ID NO:1 and / or SEQ ID NO:2. Through research, this invention confirms that the maize white spot disease resistance QTLs qMWS4-1 and qMWS4-5 regions co-localize with the resistance SNP:4-118983592, and both have a strong phenotypic explanatory power for maize white spot disease resistance. The associated gene Zm00001eb181850 (SEQ ID NO:2) is highly expressed in maize leaves, proving its association with maize leaf spot disease. Maize white spot disease resistance is a quantitative trait jointly regulated by multiple genes. The technical solution provided by this invention offers a new method for breeding new maize varieties resistant to white spot disease using modern molecular breeding techniques.

The application belongs to the technical field of molecular marker assisted breeding, and particularly relates to a corn chromosome 4 white spot disease resistance related molecular marker and application, specifically, the application provides a corn white spot disease resistance related molecular marker, the molecular marker gene is a Zm00001eb168510 gene and / or a sequence as shown in SEQ ID NO:1, a CDs sequence of the Zm00001eb168510 gene is as shown in SEQ ID NO:2, through years of experiments in multiple points, the application is co-located to a white spot disease resistance QTL qMWS4-4 interval and a resistance SNP:4-14146725 and an associated gene Zm00001eb168510 on a corn chromosome 4, the SNP site can be located in different environments through QTL linkage analysis and GWAS correlation analysis, and can explain 15.14% of the phenotype variation; the associated candidate functional gene mainly encodes a cysteine protease superfamily protein, the cysteine protease superfamily contains a deubiquitinating enzyme, and plays an important role in plant development and adversity response.

The invention relates to the field of aquaculture, in particular to a preparation method and application of a large yellow croaker visceral white-spot disease recombinant live vector vaccine. In order to solve the problem in the industry of prevention and control of the visceral white-spot disease of the large yellow croaker, 5-type replication-defective adenovirus is selected as a gene expression vector, a recombinant adenovirus vector comprising a plurality of outer membrane proteins of pseudomonas plecoglossicida is constructed in a series connection manner, and a recombinant virus is obtained; the safe and effective recombinant live vector vaccine for the visceral white-spot disease of the pseudosciaena crocea is created. According to the vaccine, the larimichthys crocea is immunized by adopting a soaking or bait feeding mode, the CPM of the larimichthys crocea immunized by the vaccine is 24.0%, and the CPM of the fish which is not immunized by the vaccine is 88.0%. In addition, RPS (relative survival percentage) of inoculated fish and empty carrier control fish are respectively 72.73% and 9.09%. The method is at a leading level in the field of pseudosciaena crocea biomedicine at present, and creativity is remarkable.

The invention provides lactococcus lactis R3 and application thereof in preventing and treating visceral white-spot disease of large yellow croakers. The strain is classified and named as lactococcus lactis R3, and is preserved in the China Center for Type Culture Collection (CCTCC) on October 21, 2024, and the preservation number is CCTCC M 20242278. The strain is separated and identified from healthy large yellow croaker intestinal tracts and has antibacterial activity and non-hemolytic activity, antibacterial tests show that the lactococcus lactis R3 is wide in antibacterial spectrum and good in biocontrol effect, and fermentation liquor of the lactococcus lactis R3 can effectively inhibit pseudomonas proteinus, aeromonas hydrophila, escherichia coli, vibrio parahaemolyticus and vibrio alginolyticus. The thallus or the fermentation liquor is used as a feed additive, so that the effect of preventing and treating the visceral white-spot disease can be achieved, a new idea is provided for replacing antibiotics to treat the visceral white-spot disease, and a reliable standby strain is also provided for a fish-source microecological preparation.

The invention discloses a corn leukoderma resistance gene MWSR1.1, a molecular marker and application. A nucleotide sequence of a coding region of the gene MWSR1.1 is as shown in SEQ ID NO: 1; the nucleotide sequence of the molecular marker primer is as shown in SEQ ID NO: 7-10. The SEQ ID NO: 1 of the MWSR1.1 gene is a haplotype capable of remarkably improving the corn leukoderma resistance, the result of the invention is beneficial to further research on the regulation mechanism of the corn leukoderma resistance, and a theoretical basis is provided for breeding new varieties of corn with high leukoderma resistance.

The invention provides a specific primer group for detecting Pseudomonas plecoglossicida, which comprises specific primers, and the specific primers comprise an upstream primer P.p-RPA-F, a downstream primer P.p-RPA-F, an upstream primer P.p-RPA-F, a downstream primer P.p-RPA-F, a downstream primer P.p-RPA-F, a downstream primer P.p-RPA-F and a downstream primer P.p-RPA-F, and the sequence of the downstream primer P.p-RPA-R is as shown in SEQ ID NO: 2. The invention further provides a corresponding kit, application and a method. The specific primer group and the kit are suitable for rapid detection of the visceral white-spot disease of the pseudosciaena crocea in a basic laboratory and a pseudosciaena crocea farm site, the rapid propagation and diffusion risk of the new pathogen is reduced, the prevention and control effect on the visceral white-spot disease is improved, and a technical support is provided for detection and disease diagnosis of the visceral white-spot disease of the pseudosciaena crocea.

The present invention belongs to the technical field of molecular marker-assisted breeding, and specifically relates to molecular markers associated with white spot disease resistance on maize chromosome 8 and their applications. The molecular marker gene sequence located on maize chromosome 8 is shown in SEQ ID NO: 1 and / or SEQ ID NO: 2. Through years of multi-point experiments, the present invention co-localizes SNP: 8-149153434 and its associated gene Zm00001eb358700 (SEQ ID NO: 2) on maize chromosome 8. The SNP can be located under multiple different environments and can explain 22.5% of phenotypic variation. This site can be used as a molecular marker for maize white spot disease resistance.

This invention provides an application of the Zm00001eb155730 gene in maize breeding, relating to the field of agricultural biotechnology. The Zm00001eb155730 gene is applied to marker-assisted breeding to regulate maize white spot disease resistance. This invention utilizes genome-wide association analysis (GWAS) and quantitative trait locus analysis of genetic linkage maps to locate S3-210892574 on chromosome 3, which is significantly associated with maize white spot disease resistance, thereby identifying the functional gene Zm00001eb155730 that regulates maize white spot disease resistance. The Zm00001eb155730 gene exhibits a dominant haplotype in GWAS, and this haplotype shows significantly higher resistance to white spot disease than other haplotypes, providing a theoretical basis for marker-assisted selection to regulate maize white spot disease resistance.

The invention relates to the use of bacterial lipopeptide biosurfactants in the treatment of white spot disease in fresh water and marine fish. Particularly useful for treatment of white spot disease are viscosin-like lipopeptide biosurfactants obtainable from the Pseudomonas fluorescens strain H6, massetolide or a derivative thereof and putisolvin or a derivative thereof.

The invention discloses a corn leukoderma resistance gene MWSR3.1, a molecular marker and application, a nucleotide sequence of a coding region of the corn leukoderma resistance gene MWSR3.1 is as shown in SEQ ID NO: 1, and nucleotide sequences of primers of the molecular marker are as shown in SEQ ID NO: 7-10. The SEQ ID NO: 1 of the MWSR3.1 gene is a haplotype capable of remarkably improving the corn leukoderma resistance, the research result is beneficial to further research on the regulation mechanism of the corn leukoderma resistance, and a theoretical basis and resources are provided for breeding new corn varieties with high leukoderma resistance.

The present invention provides a strain of Lactobacillus plantarum E2 and its application in preventing and treating white spot disease of large yellow croaker viscera. The strain is classified and named as Lactobacillus plantarum ( Lactobacillus plantarum ) E2 was deposited with the China Center for Type Culture Collection on December 29, 2022, at 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province, with the accession number CCTCC NO: M 20222097. Antibacterial tests have shown that Lactobacillus plantarum E2 has a broad antibacterial spectrum and excellent biocontrol efficacy. Its fermentation broth effectively inhibits Pseudomonas aeruginosa, Vibrio harveyi, Vibrio alginolyticus, Vibrio Campbellii, and Aeromonas hydrophila. Using the bacteria or fermentation broth as a feed additive can prevent and treat visceral white spot disease and vibriosis.