57 results about "White spot disease" patented technology

The present invention discloses a compound Chinese herbal medicine preparation for preventing and curing white spot disease (WSD) of procambarus clarkia and a method thereof. The compound Chinese herbal medicine preparation comprises the following components: radix isatidis, rheum officinale, herba houttuyniae and allicin. The method comprises the following steps: the compound Chinese herbal medicinal preparation is mixed with feed and the feed is continuously used to feed for 30-40 days before a season of an onset of the WSD of the procambarus clarkia. Compared with the prior art, especially for ponds which the WSD outbreak, the provided compound Chinese herbal medicine preparation for preventing and curing the WSD of the procambarus clarkia can control the incidence of the WSD of the procambarus clarkia to a very low level, the method is simple in operations, and the preparation has very important meanings for preventing and curing the WSD of the procambarus clarkia, reducing morbidity of the procambarus clarkia caused by the WSD, and improving yields.

The invention discloses a mixed aquaculture mode for fishes and shellfish in a seawater pond and relates to the technical field of aquaculture. The mixed aquaculture mode is realized through steps of pond selection, aquaculture variety and number selection, aquaculture management and pond water management. According to the mixed aquaculture mode for fishes and shellfish in the seawater pond, mixed aquaculture is carried out on large yellow croaker and Chinese razor clam, and moreover, filter feeding mullet and bottom shellfish are cultured together, the aquaculture capacity is completely used, water full of fertilizer generated by the large yellow croaker aquaculture serves as the bait of the Chinese razor clam, the water full of fertilizer is discharged after the Chinese razor clam completely absorbs the alga therein, the multi-level large yellow croaker bait nutrition absorption and use are realized, the spending on the purchase of fertilizer for the Chinese razor clam is saved, and the rich nutrition level of the discharged water is greatly reduced; fresh water is continuously filled into the pond to lower the salinity, the white-spot disease of the large yellow croaker is effectively avoided, the large yellow croaker and Chinese razor clam benefit from each other, and the mixed aquaculture mode is an effective and energy-saving aquaculture mode.

The invention relates to a method for removing residual cryptocryon irritans from a culture pond in a pollution-free way. The method comprises the following steps of (1) checking body surfaces, gills and fins of fishes in a pond A, judging whether the fishes suffer from 'white spot disease', controlling the water temperature to be between 25 and 30 DEG C, and observing the number of white spots on the bodies of the fishes in two to three consecutive days; (2) when the white spots are obviously reduced, pouring all fishes in the pond A into a pond B; (3) charging air into water in the pond A for 48 to 72 hours without any additional treatment; (4) controlling the water temperature of the pond B to be between 25 and 30 DEG C, and pouring all fishes into a pond C 48 hours after the fishes are poured into the pond B, wherein all trophozoites remaining on the bodies of the fishes are basically removed 48 hours after the fishes are poured into the pond B; and (5) continuously charging air into water in the pond B for 48 to 72 hours without any additional treatment. After all steps are taken, almost all cryptocryon irritans living on the bodies of the fishes are removed, and almost all larvae incubated by cysts in the ponds A and B are killed. The method is low in cost and simple in operation, and is environment-friendly and pollution-free because no chemical medicine is used.

The invention relates to the use of bacterial lipopeptide biosurfactants in the treatment of white spot disease in fresh water and marine fish. Particularly useful for treatment of white spot disease are viscosin-like lipopeptide biosurfactants obtainable from the Pseudomonas fluorescens strain H6, massetolide or a derivative thereof and putisolvin or a derivative thereof.

The invention relates to a Pseudomonas inhibiting composition and application thereof in the preparation of a medicine for preventing and treating pseudosciaena crocea visceral white spot disease. ThePseudomonas inhibiting composition is mainly a mixed aqueous solution of nisin Z and polymyxin B, wherein the mass ratio of nisin Z to polymyxin B (hereinafter referred to as PB) is (500 to 4000):1 and the concentration of nisinZ is 250 to 2000 mug / ml. The Pseudomonas inhibiting composition has obvious inhibiting effects on various Gram-negative bacteria, and particularly has obvious long-term inhibiting effect on the pseudosciaena crocea visceral white spot pathogenic bacteria, so as to effectively prevent and treat pseudosciaena crocea visceral white spot disease.

The invention relates to a medicine for treating a white-spot disease of golden pomfrets and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. The medicine is prepared from the following raw materials in parts by weight: 10-20 parts of stone-like omphalia, 10-20 parts of licorice root, 20-30 parts of chenopodium ambrosioides, 20-30 parts of pomegranate rind, 20-40 parts of bletilla striata, 5-15 parts of siberian cocklour fruit, 5-15 parts of heartleaf houttuynia herb, and 2-8 parts of folium isatidis. For the medicine for treating white-spot disease of golden pomfrets, according to the mechanism of action of the white-spot disease, appropriate traditional Chinese medicines are selected and organically combined to achieve the effect of complementing each other, and the function of each component is furthest exerted. The traditional Chinese medicine composition is safe and reliable, is harmless to the environment, and has a significant effect on preventing and treating white-spot disease of golden pomfrets, and the survival rate of the sickened golden pomfrets achieves more than 98%.

The invention aims to overcome the defects of the prior art, and provides the medicine for effectively treating prawn leukoderma, which is safe to use, green and environment-friendly. According to the technical scheme, the Chinese herbal medicine composition for effectively treating the prawn white spot disease is characterized by comprising the following components in parts by weight: 45-55 parts of honeysuckle, 45-55 parts of radix isatidis, 25-35 parts of astragalus membranaceus, 15-25 parts of fructus forsythiae, 20-35 parts of golden cypress, 15-25 parts of liquorice and 15-25 parts of angelica sinensis. According to the traditional Chinese medicine monarch, minister, assistant and guide scientific compatibility theory, the effective components of most of the medicinal materials are quantitatively and semi-quantitatively detected by means of modern science and technology, the prepared finished product has a good treatment effect on the prawn white spot disease, all the medicinal materials act synergistically, the inhibition effect on pathogenic viruses is enhanced, the toxicity of a single medicinal material is reduced, and the curative effect of the prawn white spot disease is improved. The traditional Chinese medicine has a remarkable prevention and treatment effect on prawn white spot, is a traditional Chinese medicine which is good in curative effect, free of pollution and residues and convenient to use, and is particularly suitable for industrial large-scale prawn culture.

The invention discloses a white spot disease virus single-chain variable region recombinant antibody as well as a preparation method and application thereof. A strain WSSV-VP28-3B7-scFv for efficiently expressing the protein is prepared by cloning an scFv gene sequence of the anti-WSSV-VP28 neutralizing monoclonal antibody 3B7, so that the single-chain variable region recombinant antibody of the WSSV-VP28 neutralizing monoclonal antibody is obtained. The recombinant single-chain variable region antibody can be specifically combined with and recognize natural protein of the envelope protein VP28 of the white spot disease virus, also can recognize white spot disease virions, and can effectively neutralize the white spot disease virus and delay prawn death caused by the virus. The prepared single-chain variable region antibody of the white spot disease virus envelope protein VP28 neutralizing monoclonal antibody provides an important tool for research on rapid detection, diagnosis and prevention of white spot disease viruses based on the single-chain variable region antibody.

The invention discloses a mehtod for detecting shrimp white spot disease virus based on a liquid chip. The invention provides a specific primer pair for assisting in identification of the shrimp white spot disease virus, which comprises single-stranded DNA (deoxyribonucleic acid) molecules as shown in a sequence 1 in a sequence table and the single-stranded DNA molecules as shown in a sequence 2 in the sequence table. The invention further protects a primer probe composition for assisting in identification of the shrimp white spot disease virus, which comprises the specific primer pair and a probe T1, wherein the nucleotide sequence of the probe T1 is as shown in a sequence 3 in the sequence table. The specific primer pair provided by the invention has good specificity when being used for identifying the shrimp white spot disease virus. The primer probe composition combined liquid chip provided by the invention has the advantages of good specificity, high sensitivity, simplicity and convenience in operation, short time, no environmental pollution, no threat to human health and high-throughput detection when being used for identifying the shrimp white spot disease virus. The method disclosed by the invention is very suitable for detecting import and export aquatic animals.

The invention discloses ornamental fish bait with functions of prevention and treatment of a white spot disease. The ornamental fish bait comprises, by weight, 100 parts of soybean powder, 32 to 35 parts of pepper powder, 20 to 30 parts of peanut powder, 22 to 25 parts of pig nail powder, 15 to 20 parts of sweet potato powder, 16 to 19 parts of shrimp powder, 13 to 15 parts of azobisformamide, 3 to 5 parts of quick lime and 2 to 5 parts of cactus liquid. According to the ornamental fish bait with the functions of prevention and treatment of the white spot disease, the soybean powder and the pepper powder are utilized as main ingredients, the components such as the pig nail powder, the quick lime and the cactus liquid are adopted, and accordingly the prominent curative effect on the white spot disease is achieved and meanwhile the bait absorption of a sick body can be prompted due to the peanut powder, the sweet potato powder, the shrimp powder and the azobisformamide and accordingly the effect of twice the result with half the effort can be achieved.

The invention discloses a method for extracting genome DNA of prawn white spot baculovirus. It takes the surviving sick shrimp or fresh dead shrimp suffering from white spot baculovirus disease as samples, washes them with sterile water first, then puts the samples in a heating vessel, adds sterile water and immerses them to 2-3cm, and heats them. After boiling, keep for 8-10 minutes, add TN buffer and grind to make a homogenate, and process through medium-speed centrifugation and other steps to obtain WSSV genomic DNA. Because the present invention adopts the method of heating and boiling to pretreat the sample, the sample is easily ground, and the homogenate is rapid and sufficient; also because after the sample is heated, the animal protein, including the viral protein, coagulates and denatures, and the nucleocapsid of the virus particle is easy to separate from the tissue, The DNA yield is greatly improved; at the same time, for the early detection of diseased shrimp such as seedlings, whole shrimp can also be used, which facilitates the acquisition of samples. The invention provides a fast, convenient and effective way for extracting WSSV genome DNA.

The invention utilizes white spot syndrome virus (WSSV) gene expression technology to obtain a protein coded by white spot caculovirus WSV237 gene, and the protein is named VP292. The invention also relates to a method for producing VP292, application of VP292 polynucleotide and polypeptide as well as antibody specifically combined with VP292 polypeptide. Inhibition on the gene and expression product thereof can be one effective way for preventing and treating white spot disease, thus being hopeful to be applied to preventing and treating white spot disease.