58 results about "White spot disease" patented technology

The present invention discloses a compound Chinese herbal medicine preparation for preventing and curing white spot disease (WSD) of procambarus clarkia and a method thereof. The compound Chinese herbal medicine preparation comprises the following components: radix isatidis, rheum officinale, herba houttuyniae and allicin. The method comprises the following steps: the compound Chinese herbal medicinal preparation is mixed with feed and the feed is continuously used to feed for 30-40 days before a season of an onset of the WSD of the procambarus clarkia. Compared with the prior art, especially for ponds which the WSD outbreak, the provided compound Chinese herbal medicine preparation for preventing and curing the WSD of the procambarus clarkia can control the incidence of the WSD of the procambarus clarkia to a very low level, the method is simple in operations, and the preparation has very important meanings for preventing and curing the WSD of the procambarus clarkia, reducing morbidity of the procambarus clarkia caused by the WSD, and improving yields.

The invention discloses a triple vaccine special for Muscovy duck, and the triple vaccine special for Muscovy duck uses Muscovy duck liver white-spot disease attenuated MWCA strain, Muscovy duck parvovirus attenuated P1 strain and Muscovy duck goose-origin parvovirus attenuated D strain as seed viruses, and respectively uses SPF chicken embryo fibroblast or Muscovy duck embryo fibroblast spinner cultivation technology to propagate virus and obtain the cell toxic liquid, and after mixing according to a proper proportion, a freeze-drying protective agent is added for freeze drying, and the triple live vaccine of Muscovy duck reovirus disease, Muscovy duck parvovirus disease and Muscovy duck gosling plague special for Muscovy duck is prepared; the triple vaccine can be used in the Muscovy duck culture region where the Muscovy duck reovirus disease, the Muscovy duck parvovirus disease and the Muscovy duck gosling plague are prevalent, and the purpose for preventing and controlling three diseases above can be realized by one-time immunization.

The invention discloses preparation and application of a combined vaccine for a muscovy duck parvovirus disease and a muscovy duck reovirus disease; the combined vaccine adopts a muscovy duck parvovirus attenuated P1 strain (preservation number: CCTCC V201013) and a muscovy duck liver white-spot disease (muscovy duck reovirus disease) attenuated MWCA strain (preservation number: CGMCC 0667) as seed viruses; the seed viruses are proliferated by using muscovy duck embryo fibroblasts and SPF chicken embryo fibroblasts through culture in spinner flasks; the cell viral liquid is gained, mixed according to a proper proportion, and freeze-dried by adding a freeze-drying protective agent to obtain the combined live vaccine; the combined live vaccine is applicable to muscovy duck breeding areas with epidemic muscovy duck three-week diseases and liver white-spot diseases.

The invention discloses a mixed aquaculture mode for fishes and shellfish in a seawater pond and relates to the technical field of aquaculture. The mixed aquaculture mode is realized through steps of pond selection, aquaculture variety and number selection, aquaculture management and pond water management. According to the mixed aquaculture mode for fishes and shellfish in the seawater pond, mixed aquaculture is carried out on large yellow croaker and Chinese razor clam, and moreover, filter feeding mullet and bottom shellfish are cultured together, the aquaculture capacity is completely used, water full of fertilizer generated by the large yellow croaker aquaculture serves as the bait of the Chinese razor clam, the water full of fertilizer is discharged after the Chinese razor clam completely absorbs the alga therein, the multi-level large yellow croaker bait nutrition absorption and use are realized, the spending on the purchase of fertilizer for the Chinese razor clam is saved, and the rich nutrition level of the discharged water is greatly reduced; fresh water is continuously filled into the pond to lower the salinity, the white-spot disease of the large yellow croaker is effectively avoided, the large yellow croaker and Chinese razor clam benefit from each other, and the mixed aquaculture mode is an effective and energy-saving aquaculture mode.

The invention relates to a method for removing residual cryptocryon irritans from a culture pond in a pollution-free way. The method comprises the following steps of (1) checking body surfaces, gills and fins of fishes in a pond A, judging whether the fishes suffer from 'white spot disease', controlling the water temperature to be between 25 and 30 DEG C, and observing the number of white spots on the bodies of the fishes in two to three consecutive days; (2) when the white spots are obviously reduced, pouring all fishes in the pond A into a pond B; (3) charging air into water in the pond A for 48 to 72 hours without any additional treatment; (4) controlling the water temperature of the pond B to be between 25 and 30 DEG C, and pouring all fishes into a pond C 48 hours after the fishes are poured into the pond B, wherein all trophozoites remaining on the bodies of the fishes are basically removed 48 hours after the fishes are poured into the pond B; and (5) continuously charging air into water in the pond B for 48 to 72 hours without any additional treatment. After all steps are taken, almost all cryptocryon irritans living on the bodies of the fishes are removed, and almost all larvae incubated by cysts in the ponds A and B are killed. The method is low in cost and simple in operation, and is environment-friendly and pollution-free because no chemical medicine is used.

The invention discloses a compound Chinese herbal medicine capable of treating white spot disease of large yellow croaker, and a manufacturing method thereof. The compound Chinese herbal medicine capable of treating the white spot disease of large yellow croaker comprises six Chinese herbal medicines with a ratio of rheum officinale : cyrtomium rhizome : golden cypress : radix stemonae : Chinese gall : dried ginger being 22 : 13 : 12 : 15 : 9 : 15. The manufacturing method of the compound Chinese herbal medicine capable of treating the white spot disease of large yellow croaker comprises the following specific steps of (1) extracting by decoction for a first time; (2) decocting for a second time; (3) carrying out double-effect concentration; and (4) spray drying. The compound Chinese herbal medicine capable of treating the white spot disease of large yellow croaker is prepared by compounding the six Chinese herbal medicines and by adopting high pressure decoction, double-effect concentration and spray drying, so that efficacy and palatability of the medicine are greatly improved. The number of the white spot disease of large yellow croaker is significantly reduced after the compound Chinese herbal medicine is fed to the large yellow croaker.

The invention relates to the technical field of prevention and control of aquaculture diseases and particularly relates to a medicine for treating a small melon worm disease of aquatic animals as well as a preparation method and an application method of the medicine. The medicine for treating a mystus guttatus white-spot disease is composed of a first-stage medicine, a second-stage medicine and a third-stage medicine; the first-stage medicine is an acetic acid solution; the second-stage medicine is a methylene blue solution; the third-stage medicine is prepared from chilies and fresh gingers. By using the medicine to carry out staging treatment, Ichthyophthirius on fish bodysurfaces and in vesicae are completely killed in one step and the possibility that the Ichthyophthirius reappear is avoided; the medicine is ecological and environmentally friendly, does not damage the environment, is harmless to human bodies and no medicine residues exist in fish bodies.

The invention discloses a medicine for treating white spot disease of turtle. The medicine consists of gentian violet, fructus forsythiae, blackberry lily, sodium chloride, houttuynia cordata, honeysuckle, amur cork tree bark and common cnidium fruit. When resisting fungi, the medicine disclosed by the invention has a certain capacity of inhibiting aeromonas, so that the medicine can be used for treating white spot disease of turtle. Through reasonable combination, dried orange peel, hawthorn, houttuynia cordata, honeysuckle, wild chrysanthemum, amur cork tree bark and the like can eliminate the heavy flavor of garlic when resisting the fungi. Liquorice root can be used for protecting liver so as to protect liver cells from being hurt by tannin of gallnut and the like. Through a preparation method, an active ingredient extraction ratio is improved and the loss of beneficial materials is reduced. The garlic is mixed with the dried orange peel, so that the flavor of the garlic is eliminated in advance.

The invention discloses bacteriophage of fish bacteria and application thereof. The bacteriophage is named as Aeromonassa schubertii bacteriophage SD04. The bacteriophage has the advantages that the bacteriophage SD04 is used for treating white spot diseases of inner organs of channa maculatae due to infection of aeromonas schubertii for the first time, and the natural infection mode of the bacteria is simulated; the bacteriophage is sprayed to prevent the white spot diseases, the disease treatment mode of abuse of antibiotics and chemical drugs is broken through, the possible multiple drug-resistant bacteria infection, even threat of 'super-bacteria', and pollution and destruction destroy to breedingculture environment and ecological environment are reduced for the breedingculture industry, and a foundation is laid for treatment of the bacteria diseases by using the bacteriophage as a biological agentpreparation in the later study.

The invention discloses a medicine for treating white-spot disease caused by aeromonas. The medicine comprises fructus forsythiae, licorice root, garlic, Chinese gall, radix isatidis, rheum officinale, lonicera japonica, chrysanthemum indicum, ash bark, herba houttuyniae, Japanese buttercup herb, coptis chinensis, radix scutellariae, cortex phellodendri, pericarpium citri reticulatae, medicine terminalia fruit, sweet wormwood, rhizoma belamcandae, alisma plantago-aquatica linn, angelica sinensis, asparagus fern, fructus mume, pangolin, flos paulowniae, Japanese cayratia herb, solanum lyratum thunb and radices trichosanthis. The traditional Chinese medicine can be used for treating white-spot disease with a remarkable curative effect, and the curative rate of 30 days of the traditional Chinese medicine can be 90 percent. Garlic and other materials having spicy flavor are used, but other medicinal materials are reasonably combined, so that the aim of removing smell without removing curative effects can be achieved, and the curative effect can be further improved when the medicine is especially combined with western medicine antibiotics and added with calcined gypsum and other components.

The invention belongs to the technical field of aquaculture, and relates to a feed additive for preventing white-spot disease of parrot fishes. The invention also relates to a preparation method of the feed additive for preventing the white-spot disease of the parrot fishes. The feed additive for preventing the white-spot disease of the parrot fishes comprises the following components in parts by weight: 36-42 parts of calamus, 30-35 parts of sweet potatoes, 28-35 parts of Chinese honey locust, 28-34 parts of honeysuckle, 28-34 parts of peach leaves, 27-34 parts of garden burnet roots, 25-33 parts of lavender, 24-32 parts of sculellaria barbata, 24-30 parts of schisandra chinensis, 21-27 parts of capillary wormwood herb, 21-27 parts of patrinia scabiosaefolia, 20-26 parts of rush juncus effusus roots, 19-27 parts of Lip-fern, 19-25 parts of acacia catechu, 14-21 parts of garlic and 12-20 parts of hedyotis diffusa. With the adoption of the feed additive disclosed in the invention, the white-spot disease of the parrot fishes can be effectively prevented and treated.

The invention provides a composition with immunization-enhancing or prevention effect on white spot syndrome and application thereof. The composition comprises extractives of the following six cereals: corn, barley, Job's tears, millet, buckwheat and wheat, comprises extractives of the following 25 Chinese herbal medicines: medicated leaven, fructus hordei germinatus, radix et rhizoma rhei, cablin potchouli herb, radix glycyrrhizae, folium perillae, rhizoma atractylodis macrocephalae, rhizoma chuanxiong, radix scutellariae, chinese lizardtail rhiaomeor herb, fructus crataegi, radix angelicae sinensis, radix ginseng rubra, fructus lycii, rhizoma coptidis, radix astragali, fructus schisandrae Chinensis, concha haliotidis, herba artemisiae annuae, poria, cortex magnoliae officinalis, fructus corni, radix aucklandiae, akebia stem and fructus forsythia, comprises the following four algae extractives: laminaria japonica, scagassum, codium fragile and seaweed, and comprises the following three mushroom mycelia: tricholoma gambosum mycelium, lentinus edodes mycelium and cordyceps sinensis mycelium. The composition is applicable as a forage additive. The composition has the advantages of promoting growth of mammalia, poultry, fish, arthropods and most of animals, enhancing resistance and the like, and especially has special efficacy for preventing white spot syndrome of crustacea.

The invention relates to the use of bacterial lipopeptide biosurfactants in the treatment of white spot disease in fresh water and marine fish. Particularly useful for treatment of white spot disease are viscosin-like lipopeptide biosurfactants obtainable from the Pseudomonas fluorescens strain H6, massetolide or a derivative thereof and putisolvin or a derivative thereof.

The invention utilizes prawn white spot syndrome virus (WSSV) gene expression technology to obtain a segment which is coded by a prawn white spot bacilliform virus WSV254 gene and is named as VP37p. The invention also relates to a method for manufacturing the VP37p, application of polynucleotide and polypeptide of the VP37p, and an antibody specifically bound with the polypeptide of the VP37p. The invention identifies the function of the VP37p through a biological method for the first time; the VP37p is one of WSSV attachment protein fragments; and the inhibition on the gene and expression products thereof may be one of effective ways of preventing and controlling prawn white spot disease and expects to be applied to the prevention and control of the prawn white spot disease.

The invention discloses a Chinese herba preparation for preventing and treating the white-spot disease of a Chinese softshell turtle and a preparation method thereof. The Chinese herba preparation is medicine powder which is formed by traditional Chinese raw materials comprising, by weight, 25% of Chinese gall, 15% of Scutellaria baicalensis, 15% of Chinese violet, 10% of gynura segetum, 15% of radix sophorae flavescentis, 10% of garden burnet and 10% of cortex pseudolaricis, wherein the sum of all the ingredients is 100%. The Chinese herba preparation for preventing and treating the white-spot disease of the Chinese softshell turtle has the advantages of being obvious in treating effect on the white-spot disease of the Chinese softshell turtle and low in morbidity.

The present invention clones one uracil deoxyribonucleoside triphosphatase (dUTPase) gene from prawn white spot baculovirus (WSBV) genome. The gene has nucleotide sequence encoding one kind of polypeptide with dUTPase activity. The present invention also relates to the application of dUTPase gene and the encoded polypeptide and the production process of the said active polypeptide. The present invention identifies the function of dUTPase gene of WSBV virus in biological method. Because dUTPase gene is one of the key enzyme for nucleotide synthesis and metabolism, the fast copying and proliferation of WSBV and the inhibition on the enzyme may be one of the effective way of preventing and treating prawn white spot disease and is expected to be used in the prevention and treatment of prawn white spot disease.

The present invention discloses a leech white spot disease prevention and control feed, which is prepared from the following raw materials by weight: 30-40 parts of peanut meal, 50-60 parts of chicken giblets, 10-15 parts of white phaseolus vulgaris linn, 2-3 parts of curry powder, 2-3 parts of edible salt, 6-7 parts of edible oil, 10-15 parts of citrus sinensis peel, 40-50 parts of camellia oleifera abel cake, 30-40 parts of blood powder, 10-15 parts of zea mays leaf, 6-7 parts of alpha-starch, 50-60 parts of fish cream, a proper amount of nanometer montmorillonite, a proper amount of yeast, a proper amount of Bacillus natto, a proper amount of edible vinegar, a proper amount of ammonia water, and a proper amount of water. According to the present invention, the prepared feed is easily digested with the treated camellia oleifera abel cake, the nutritional value of the cake meal is improved, and the flavor of the cake meal is improved; the edible salt can provide effects of virus killing and sterilization, and the nanometer montmorillonite can provide effects of effective adsorption, inactivation and degradation of various moulds and toxins; and the bacterial infection of leeches can be reduced, and the feed has a good leech white spot disease prevention and control effect.

The invention discloses a traditional Chinese medicine preparation for treating stress diseases of Chinese softshell turtles and a preparation method of the preparation. The traditional Chinese medicine preparation is powder prepared from the following medicinal materials in percentage by weight: 5-11% of ligusticum chuanxiong hort, 5-11% of salvia miltiorrhiza, 2-8% of angelicae sinensis, 2-8% of leonurus, 7-13% of uncaria rhynchophylla, 7-13% of rhizoma corydalis, 5-11% of jujube seed, 5-11% of radix bupleuri, 5-11% of polygonatum sibiricum, 5-11% of astragalus membranaceus, 5-11% of epimedium, 5-11% of schisandra chinensis, 2-8% of radix glycyrrhizae, and 0.5-1.5% of vitamin C; the total percentage of all components is 100%. The traditional Chinese medicine preparation for treating stress diseases of Chinese softshell turtles, provided by the invention, has the characteristics of being good in curative effect on treating the white spot disease of the Chinese softshell turtles and free of drug residues.

The invention relates to a Pseudomonas inhibiting composition and application thereof in the preparation of a medicine for preventing and treating pseudosciaena crocea visceral white spot disease. ThePseudomonas inhibiting composition is mainly a mixed aqueous solution of nisin Z and polymyxin B, wherein the mass ratio of nisin Z to polymyxin B (hereinafter referred to as PB) is (500 to 4000):1 and the concentration of nisinZ is 250 to 2000 mug / ml. The Pseudomonas inhibiting composition has obvious inhibiting effects on various Gram-negative bacteria, and particularly has obvious long-term inhibiting effect on the pseudosciaena crocea visceral white spot pathogenic bacteria, so as to effectively prevent and treat pseudosciaena crocea visceral white spot disease.

The invention relates to a medicine for treating a white-spot disease of golden pomfrets and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. The medicine is prepared from the following raw materials in parts by weight: 10-20 parts of stone-like omphalia, 10-20 parts of licorice root, 20-30 parts of chenopodium ambrosioides, 20-30 parts of pomegranate rind, 20-40 parts of bletilla striata, 5-15 parts of siberian cocklour fruit, 5-15 parts of heartleaf houttuynia herb, and 2-8 parts of folium isatidis. For the medicine for treating white-spot disease of golden pomfrets, according to the mechanism of action of the white-spot disease, appropriate traditional Chinese medicines are selected and organically combined to achieve the effect of complementing each other, and the function of each component is furthest exerted. The traditional Chinese medicine composition is safe and reliable, is harmless to the environment, and has a significant effect on preventing and treating white-spot disease of golden pomfrets, and the survival rate of the sickened golden pomfrets achieves more than 98%.

The invention belongs to the technical field of aquaculture, and particularly relates to an antidote for aquatic products and a preparation method of the antidote. The antidote comprises the followingcomponents: bacillus subtilis, saccharomycetes, a metal complexing agent, diatomite, antibacterial peptide, radix sophorae flavescentis, semen hydnocarpi, folium pini, fructus rhois chinensis, honeysuckle, houttuynia cordata and galla chinensis. The antidote aims to solve the problem that aquatic animal bodies contain heavy metals, antibiotics, pesticide residues and heavy metals, has a definitedetoxifying effect on cultured varieties damaged by excessive water toxin, can degrade nitrite and improve water quality, is long in lasting period, is safe and environmentally friendly, meanwhile canprevent and treat the white spot disease of lobsters, and has a food calling effect.

The invention aims to overcome the defects of the prior art, and provides the medicine for effectively treating prawn leukoderma, which is safe to use, green and environment-friendly. According to the technical scheme, the Chinese herbal medicine composition for effectively treating the prawn white spot disease is characterized by comprising the following components in parts by weight: 45-55 parts of honeysuckle, 45-55 parts of radix isatidis, 25-35 parts of astragalus membranaceus, 15-25 parts of fructus forsythiae, 20-35 parts of golden cypress, 15-25 parts of liquorice and 15-25 parts of angelica sinensis. According to the traditional Chinese medicine monarch, minister, assistant and guide scientific compatibility theory, the effective components of most of the medicinal materials are quantitatively and semi-quantitatively detected by means of modern science and technology, the prepared finished product has a good treatment effect on the prawn white spot disease, all the medicinal materials act synergistically, the inhibition effect on pathogenic viruses is enhanced, the toxicity of a single medicinal material is reduced, and the curative effect of the prawn white spot disease is improved. The traditional Chinese medicine has a remarkable prevention and treatment effect on prawn white spot, is a traditional Chinese medicine which is good in curative effect, free of pollution and residues and convenient to use, and is particularly suitable for industrial large-scale prawn culture.

The invention discloses a white spot disease virus single-chain variable region recombinant antibody as well as a preparation method and application thereof. A strain WSSV-VP28-3B7-scFv for efficiently expressing the protein is prepared by cloning an scFv gene sequence of the anti-WSSV-VP28 neutralizing monoclonal antibody 3B7, so that the single-chain variable region recombinant antibody of the WSSV-VP28 neutralizing monoclonal antibody is obtained. The recombinant single-chain variable region antibody can be specifically combined with and recognize natural protein of the envelope protein VP28 of the white spot disease virus, also can recognize white spot disease virions, and can effectively neutralize the white spot disease virus and delay prawn death caused by the virus. The prepared single-chain variable region antibody of the white spot disease virus envelope protein VP28 neutralizing monoclonal antibody provides an important tool for research on rapid detection, diagnosis and prevention of white spot disease viruses based on the single-chain variable region antibody.

The invention discloses a combined metabolic marker and a detection kit for judging the genetic modification effect of pathogenic bacteria of visceral white-spot disease of epinephelus coioides, and relates to lipid metabolites such as phosphatidyl ethanolamine (18:2), hydroxylated fatty acid (38:2) and phosphatidylinositol (39:4) in spleen samples of epinephelus coioides as combined markers, and the method is used for judging the effect of silencing of the pseudomonas proteiniflora virulence gene clpV causing the grouper visceral white-spot disease on the grouper. The invention also relates to a kit for judging the gene modification effect. The method comprises the following steps: detecting the relative concentration of lipid metabolites in a spleen sample of grouper infected by pseudomonas proteinus, calculating a combined marker variable P based on a binary logistic regression equation, and determining a cut point value, and judging the condition of the pathogenic bacteria of the visceral white-spot disease of the grouper after genetic modification. The kit has the characteristics of high sensitivity, high detection efficiency, low cost and good repeatability, and has a good application prospect.

The invention relates to a preparation for treating visceral white-spot disease of pseudosciaena crocea and uses thereof, and belongs to the technical field of fishery. The preparation and the uses thereof are characterized in that: the preparation comprises 30-40 parts of a compound cyme extract product, 20-30 parts of a cistanche deserticola extract product, 10-15 parts of doxycycline, 4-6 parts of vitamin A, 2-5 parts of vitamin E and 6-10 parts of vitamin C. According to the preparation and the uses thereof, treating effects of the preparation for the visceral white-spot disease of the pseudosciaena crocea are good, components are compatible and synergistic, and the preparation has immunity-boosting and antimutation effects, reduces drug resistance of the pseudosciaena crocea, increases the rate of survival of the pseudosciaena crocea, accelerates the growth speed, and improves quality of fish.

The invention discloses a molecular marker-assisted breeding method of large yellow croaker capable of resisting visceral white-spot disease. The method comprises the following steps: firstly, determining an individual disease resistance phenotypic value of a reference group through an artificial challenge experiment; performing genome re-sequencing on the extreme phenotype individuals to obtain SNP sites; screening out qualified SNP (Single Nucleotide Polymorphism) sites, and filling the deleted genotypes; the significance of correlation between each SNP site and a phenotypic value is calculated through GWAS, and the most significant first 5-30 SNPs are selected as molecular markers for assistant breeding. The method comprises the following steps: carrying out individual marker identification on parent fish of a candidate group, carrying out genetic typing by virtue of MassArray or other SNP typing methods, calculating a genome estimation breeding value by virtue of a GBLUP method, selecting the parent fish according to the breeding value, and culturing the large yellow croaker capable of resisting the visceral white-spot disease. According to the method, the breeding effect can be greatly improved, large yellow croaker groups with effectively improved disease resistance can be rapidly bred, the breeding period is shortened, and the cost is reduced.

The invention discloses a mehtod for detecting shrimp white spot disease virus based on a liquid chip. The invention provides a specific primer pair for assisting in identification of the shrimp white spot disease virus, which comprises single-stranded DNA (deoxyribonucleic acid) molecules as shown in a sequence 1 in a sequence table and the single-stranded DNA molecules as shown in a sequence 2 in the sequence table. The invention further protects a primer probe composition for assisting in identification of the shrimp white spot disease virus, which comprises the specific primer pair and a probe T1, wherein the nucleotide sequence of the probe T1 is as shown in a sequence 3 in the sequence table. The specific primer pair provided by the invention has good specificity when being used for identifying the shrimp white spot disease virus. The primer probe composition combined liquid chip provided by the invention has the advantages of good specificity, high sensitivity, simplicity and convenience in operation, short time, no environmental pollution, no threat to human health and high-throughput detection when being used for identifying the shrimp white spot disease virus. The method disclosed by the invention is very suitable for detecting import and export aquatic animals.