9 results about "White spot disease" patented technology

The invention relates to a Pseudomonas plecoglossicida pldB gene deletion mutant strain and a preparation method and application thereof, and belongs to the technical field of microorganisms and the field of algae preservation, the pldB gene of the mutant strain is deleted or inactivated, the nucleotide sequence of the pldB gene is shown as SEQ ID NO: 1, and the GenBank accession number of the Pseudomonas plecoglossicida is CP031146.1. The virulence of the mutant strain is obviously reduced compared with that of a wild strain of pseudomonas plecoglossicida; the strain can induce appropriate immune response of a host and up-regulate expression of anti-inflammatory factors, is beneficial to relieving immune pathological injury, has good attenuated live vaccine potential, and provides a brand new vaccine candidate strain for prevention and control of the visceral white-spot disease of the large yellow croaker.

This invention belongs to the field of genetic breeding technology for edible and medicinal fungi, and relates to a Cordyceps militaris strain with enhanced resistance to white spot disease and its creation method. This invention provides a safe overexpression site CmSH1 (SEQ ID NO:1) in the Cordyceps militaris genome, the gene Cmhyd1 (SEQ ID NO:2) encoding a hydrophobic protein in Cordyceps militaris, a Cordyceps militaris strain with enhanced resistance to white spot disease, a method for creating the Cordyceps militaris strain with enhanced resistance to white spot disease using CRISPR / Cas9 gene editing technology, and a corresponding vector. The Cordyceps militaris strain of this invention can enhance resistance to white spot disease, and the fruiting body develops normally. Its creation method avoids food safety issues caused by the integration of transgenic elements into the genome.

The application discloses a culture device for Mesocyclops leuckarti and application thereof, and belongs to the technical field of fish culture. The culture device for Mesocyclops leuckarti comprises a cylinder, a top cover and a bottom cover, the top cover is movably connected with the top of the cylinder, and the bottom cover is fixedly connected with the bottom of the cylinder; a collecting pipe is arranged in the cylinder, the upper portion of the collecting pipe is a silk screen, the lower portion of the collecting pipe is a closed pipe, and a first light source is arranged in the collecting pipe; a second light source and a third light source are arranged on the inner side of the cylinder, and a temperature regulator and an oxygenator are arranged on the lower portion of the inner side of the cylinder. The culture device can realize large-scale continuous high-density culture of Mesocyclops leuckarti, can be applied to preparation of a biological prevention and treatment device for fish white spot disease, can reduce the abundance of water body Paramoecium caudatum and thus reduce the probability of fish suffering from the white spot disease, can provide other food sources for the fish, is beneficial to growth and development of the fish, and enriches the aquatic food web.

This invention discloses the application of Terminalia chebula in the prevention and treatment of Ichthyophthirius multifiliis (white spot disease) in aquatic animals. Toxicity experiments on grass carp kidney cells (CIK) showed that the maximum non-toxic concentration (NDC) of the aqueous extract of Terminalia chebula against CIK cells was 500 μg / mL. The inhibition of Ichthyophthirius multifiliis predator survival by the aqueous extract was dose-dependent, and the predator mortality rate reached 100% at a concentration of 100 mg / L (μg / mL). Furthermore, the median lethal concentration (LD50) of the aqueous extract against Ichthyophthirius multifiliis predators was calculated to be 14.577 mg / L. This invention is the first to propose the application of Terminalia chebula in inhibiting the survival rate of Ichthyophthirius multifiliis predators in fish at a non-toxic concentration to cells, providing a novel drug for the prevention and treatment of Ichthyophthirius multifiliis infection in aquatic animals. Terminalia chebula is a natural medicinal plant, environmentally friendly, and has the advantages of reducing the risk of drug residues.

The invention relates to a primer pair, a composition, a kit and a method for detecting pseudomonas plecoglossicida causing large yellow croaker visceral white-spot disease based on an RPA-CRISPR / Cas12a technology, the nucleotide sequences of upstream primers RPA-F are respectively as shown in SEQ ID NO.1-4, the nucleotide sequences of downstream primers RPA-R corresponding to the upstream primers RPA-F are respectively as shown in SEQ ID NO.5-8, and the composition comprises the primer pair, crRNA, Cas12a protein and a fluorescent reporter probe. Compared with the prior art, the invention establishes a P. plecoglossicida detection method which is simple and convenient to operate, short in detection time, good in specificity and high in sensitivity and only needs common constant-temperature equipment, and is suitable for on-site rapid detection in basic laboratories and large yellow croaker farms.

This invention belongs to the field of molecular genetic breeding technology, specifically relating to the molecular marker Zm00001eb181850 gene and its application related to maize white spot disease resistance. This invention provides a molecular marker related to maize white spot disease resistance, the gene sequence of which is shown in SEQ ID NO:1 and / or SEQ ID NO:2. Through research, this invention confirms that the maize white spot disease resistance QTLs qMWS4-1 and qMWS4-5 regions co-localize with the resistance SNP:4-118983592, and both have a strong phenotypic explanatory power for maize white spot disease resistance. The associated gene Zm00001eb181850 (SEQ ID NO:2) is highly expressed in maize leaves, proving its association with maize leaf spot disease. Maize white spot disease resistance is a quantitative trait jointly regulated by multiple genes. The technical solution provided by this invention offers a new method for breeding new maize varieties resistant to white spot disease using modern molecular breeding techniques.

The application belongs to the technical field of molecular marker assisted breeding, and particularly relates to a corn chromosome 4 white spot disease resistance related molecular marker and application, specifically, the application provides a corn white spot disease resistance related molecular marker, the molecular marker gene is a Zm00001eb168510 gene and / or a sequence as shown in SEQ ID NO:1, a CDs sequence of the Zm00001eb168510 gene is as shown in SEQ ID NO:2, through years of experiments in multiple points, the application is co-located to a white spot disease resistance QTL qMWS4-4 interval and a resistance SNP:4-14146725 and an associated gene Zm00001eb168510 on a corn chromosome 4, the SNP site can be located in different environments through QTL linkage analysis and GWAS correlation analysis, and can explain 15.14% of the phenotype variation; the associated candidate functional gene mainly encodes a cysteine protease superfamily protein, the cysteine protease superfamily contains a deubiquitinating enzyme, and plays an important role in plant development and adversity response.

The invention discloses a corn leukoderma resistance gene MWSR1.1, a molecular marker and application. A nucleotide sequence of a coding region of the gene MWSR1.1 is as shown in SEQ ID NO: 1; the nucleotide sequence of the molecular marker primer is as shown in SEQ ID NO: 7-10. The SEQ ID NO: 1 of the MWSR1.1 gene is a haplotype capable of remarkably improving the corn leukoderma resistance, the result of the invention is beneficial to further research on the regulation mechanism of the corn leukoderma resistance, and a theoretical basis is provided for breeding new varieties of corn with high leukoderma resistance.

The invention discloses a corn leukoderma resistance gene MWSR3.1, a molecular marker and application, a nucleotide sequence of a coding region of the corn leukoderma resistance gene MWSR3.1 is as shown in SEQ ID NO: 1, and nucleotide sequences of primers of the molecular marker are as shown in SEQ ID NO: 7-10. The SEQ ID NO: 1 of the MWSR3.1 gene is a haplotype capable of remarkably improving the corn leukoderma resistance, the research result is beneficial to further research on the regulation mechanism of the corn leukoderma resistance, and a theoretical basis and resources are provided for breeding new corn varieties with high leukoderma resistance.