309 results about "Alternaria alternata" patented technology

The invention relates to a method for controlling plant diseases caused by the fungi Botrytis cinerea and Alternaria alternata, by applying to a growing plant or to fruit or vegetables before or after harvesting, a composition which comprises an effective amount for controlling said fungi of at least one oligosaccharide ingredient, active against Botrytis cinerea and Alternaria alternata, and selected from oligosaccharides obtainable by hydrolysis of chitin, beta -glucan and other similarly active polysaccharides, excluding chitosan, of cell walls of fungi, yeasts, marine plants and exoskeletons of arthropods, The composition also forms part of the invention, as does an analogous method and composition for a method for controlling plant diseases caused by Botrytis cinerea, and utilizing at least one oligosaccharide ingredient, active against Botrytis cinerea selected from oligosaccharides obtainable by hydrolysis of chitosan, and having a molecular weight within the range of about 500 to about 10,000 daltons, provided that in this instance the composition excludes acetic acid.

The invention relates to a Bacillus amyloliquefaciense BA-KA3 and application thereof. The Bacillus amyloliquefaciense BA-KA3 is preserved in China General Microbiological Culture Collection Center on October 23, 2013, and has preservation No. CGMCC8342; its on tomato gray mold germs has obviously of antagonistic anti-role, on wheat scab bacteria. The Bacillus amyloliquefaciense BA-KA3 has obvious antagonistic effect on Fusarium graminearum, Rhizoctonia cerealis, Alternaria alternata, Phytophthora capsici, Colletotrichum capsici, Cercospora personata, Alternaria alternate and Garlic sclerotinia disease, and can significantly improve plant growth indexes such as germination rate, and budding rate and root length of seeds,and especially applies to preparation of agricultural preparations for control of tomato diseases due to Botrytiscinerea invasion and has certain growth promotion effect.

The invention relates to application and preparation of bacillus amyloliquefaciens subsp. plantarum and a bacterial agent thereof. An XH-9 bacterial strain is identified as bacillus amyloliquefaciens subsp. plantarum according to mycelial morphology, colony characteristics, physiological and biochemical indexes and 16S rDNA sequence analysis and is preserved in the China General Microbiological Culture Collection Center, and a preservation number is CGMCC NO.13151. The strain is high in antagonistic effect on pathogenic fungi such as fusarium oxysporum, bipolaris sorokiniana, fusarium pseudograminearum, colletotrichum gloeosporioides, botryosphaeria dothidea and alternaria alternata and is capable of generating heteroauxin and 1-aminocyclopropanecarboxylic acid (ACC) deaminase to stimulate plant growth and capable of degrading celluloses and can be stably colonized at rhizospheres of crops such as wheat, corn, peppers and the like. The bacterial agent prepared from the bacterial strain can be applied to prevention and treatment of root rot diseases or other soil-borne fungal diseases of crops such as wheat, corn, peppers and the like and has dual functions of disease prevention and growth promotion. The bacterial agent is simple in preparation process, short in fermentation period, low in cost and beneficial to industrial production and transport.

The invention relates to the field of pesticide preparations, and discloses a sterilization composition and application thereof. The sterilization composition disclosed by the invention comprises an alternaria tenuissima activator protein and an active ingredient A, wherein the weight ratio of the alternaria tenuissima activator protein to the active ingredient A is (1:50)-(30:1), the active ingredient A is selected from amide fungicides, strobilurin fungicides, pyrrole fungicides, triazole fungicides, pyrimidine fungicides, pyridine fungicides or carbamate fungicides, and one or more than two fungicides is selected from each type of fungicides. According to the sterilization composition, the amide fungicides, the strobilurin fungicides, the pyrrole fungicides, the triazole fungicides, the pyrimidine fungicides, the pyridine fungicides or the carbamate fungicides and the alternaria tenuissima activator protein are compounded, and have complementary advantages, thus the resistance of vegetables on diseases can be improved through once application of the sterilization composition, the yield of vegetable crops can be increased, and the amount and frequency of applying the pesticide are reduced.

The invention discloses broad-spectrum disease resisting, growth promotion and stress resisting bacillus capable of preventing and treating tomato gray mold and tomato leaf mold and application of the broad-spectrum disease resisting, growth promotion and stress resisting bacillus. The broad-spectrum disease resisting, growth promotion and stress resisting bacillus is bacillus sp. WXCDD105, and is preserved in China General Microbiological Culture Collection Center (CGMCC); the preservation address is 3, No.1 Yard, Western Beichen Road, Chaoyang District, Beijing, the preservation date is May 25, 2016, and the preservation number is CGMCC No.12496. A biocontrol strain provided by the invention has ultraviolet-resisting and drought-resisting properties, has stronger prevention and treatment effects on the tomato gray mold and the tomato leaf mold, and can inhibit fungal diseases including wilt fusarium of a plurality of types of crops, corn foot root bacteria, rice wilt pathogens, acanthopanax senticosus root rot pathogens, corynespora cassiicolai, colletrichum orbiculare, exserohilum turcicum, tomato spot blight fungi, sunflower sclerotinia sclerotiorum, botrytis cinerea, fusarium oxysporum, verticillium fusarium, alternaria alternata and the like; the broad-spectrum disease resisting, growth promotion and stress resisting bacillus has a growth promotion effect on tomato seeds and seedlings and can also improve the quality of fruits and reduce the rotting rate.

The invention relates to an environment-friendly mould-proof and anti-discoloration protective agent for rubber wood. Iodopropynyl butylcarbamate ester, an isothiazolinone compound, an organic solvent, a solubility promoter A, a solubility promoter B and water are uniformly mixed to prepare the environment-friendly mould-proof and anti-discoloration protective agent; the solubility promoter A is one of an advanced fatty acid salt surfactant, a quaternary ammonium salt surfactant and a polyhydric alcohol surfactant or a combination of the advanced fatty acid salt surfactant, the quaternary ammonium salt surfactant and the polyhydric alcohol surfactant; and the solubility promoter B is one of a sulphosalts surfactant and an isothiazolinone surfactant or a combination of the sulphosalts surfactant and the isothiazolinone surfactant. The iodopropynyl butylcarbamate ester and the isothiazolinone compound are compounded, stable treatment solution can be formed, and the environment-friendly mould-proof and anti-discoloration protective agent has the advantages of environmental protection, broad spectrum and high efficiency, is good in water solubility and stability, realizes excellent effects for preventing and treating botryodiplodia theobromae, alternaria alternata and the like which severely damage the rubber wood, is applicable to various rubber wood treatment processes such as vacuum-pressure processes, hot and cold bath processes and soaking processes, and can be directly applied to existing production processes conveniently.

The present invention relates to compositions containing the fungus Alternaria alternata and, or the secondary metabolites thereof, belonging to the family of the diketopiperazines (DKPs), for controlling the pathogen fungus Plasmopara viticola.

The invention discloses an acid-resistant pseudomonas koreensis strain CLP-7 which is capable of preventing diseases and promoting growth, and can be used for biocontrol. The acid-resistant pseudomonas koreensis strain CLP-7 is preserved at China General Microbiological Culture Collection Center, on 27th, October, 2016, and the preservation number is CGMCC No.13204. The antibacterial spectrum of the acid-resistant pseudomonas koreensis strain CLP-7 is relatively large; the acid-resistant pseudomonas koreensis strain CLP-7 is capable of inhibiting growth of Phytophthora parasitica var. nicotianae, Ralstonia solanacearum, and Alternaria alternata Keissler; the antagonistic activity under acidic conditions is high, the acid-resistant pseudomonas koreensis strain CLP-7 is capable of producing siderophore, possesses protease and glucanase activity, and potassium releasing capacity. The results of biocontrol pot experiment show that the acid-resistant pseudomonas koreensis strain CLP-7 is capable of preventing under acidic soil conditions, and promoting growth and chlorophyll synthesis of tobacco seedlings in acidic soil, so that the acid-resistant pseudomonas koreensis strain CLP-7 and microorganism bacterium agents of the acid-resistant pseudomonas koreensis strain CLP-7 can be used for preventing tobacco fungi and bacterial root and stem diseases under continuous cropping or acidic soil conditions effectively, and is high in application value.

The invention discloses an anti-bacterial protein PBR1 as well as a preparation method and application thereof, and relates to the technical field of molecular biology and biological pesticides. The amino acid sequence of the anti-bacterial protein PBR1 is shown as SEQ ID NO.1. The anti-bacterial protein PBR1 has a superior effect on preventing and curing plant diseases such as cruciferous plasmodiophoromycetes, paddy rice magnaporthe oryzae, curvularia lunata, fusarium oxysporum f. sp. dianthi, fusarium oxysporum vasinfectum, konjac foot rot bacteria, panax notoginseng root rot pathogen, pomegranate wilt pathogen, gaeumannomyces gramini, cylindrosporium pomi brooks, alternaria alternata and / or botrytis cinerea. A pesticide taking the anti-bacterial protein PBR1 as an active ingredient is a novel environment-friendly biological pesticide, is safe to people and livestock, protects the environment, and has a favorable development prospect.

The invention discloses a method for preparing ginsenoside Rg3 from alternaria alternata YS-2 and fermented ginseng stem-leaf total saponin thereof. The alternaria alternata YS-2 is an alternaria alternata YS-2 CGMCC No.3353 strain; and during fermentation, the feeding amount of the ginseng stem-leaf total saponin is 1 to 10g / L, the fermentation temperature is 10 to 50 DEG C, the pH is 2 to 8, the revolution number of a shaker is 10 to 200r / min, and the fermentation time is 5 to 120 hours. The method provides the alternaria alternata YS-2 strain with stable trait, simple nutrition requirement and good growth for scale fermentation conversion of various ginsenosides and preparation of Rg3; and the method for preparing the Rg3 by fermenting the ginseng stem-leaf total saponin through the strain is simple and convenient, and has low production cost and high product yield.

The invention discloses a Lilium regale Wilson WRKY transcription factor gene LrWRKY11. The Lilium regale Wilson WRKY transcription factor gene LrWRKY11 has a nucleotide sequence shown as SEQ ID NO: 1, and encodes a protein having an amino acid sequences shown as SEQ ID NO: 2. According to the invention, genomic studies have confirmed that the LrWRKY11 gene can improve anti-fungal functions of plants. Being constructed onto a plant expression vector and transferred into tobacco so as to be subjected to overexpression, the anti-fungal LrWRKY11 gene disclosed by the invention is capable of endowing the transgenic tobacco with very strong resistance to fungal pathogens. Experimental results have shown that transgenic tobacco with overexpression of the LrWRKY11 gene has very high level of resistance to infection of Nigrospora oryzae, Fusarium verticillioides, Botryosphaeria dothidea, Fusarium solani and Alternaria panax.

The invention discloses antibacterial and anti-virus applications of marine bacillus amyloliquefaciens 1A in fruit postpartum pathogenic fungi. The fruit postpartum pathogenic fungi are colletotrichum gloeosporioides, coniella diplodiella, pear alternaria alternata and penicillium italicum. The separating method of the marine bacillus amyloliquefaciens 1A comprises the following steps: collecting seawater, sea mud, marine debris, clam, enteromorpha and mudskipper samples in Lianyungang sea area; carrying out separation and purification of bacteria after enriching, so as to obtain a plurality of pure strains of marine bacteria; determining the inhibiting effects of the pure strains of the marine bacteria on the four fruit postpartum pathogenic fungi for a test by using a plate confrontation method; screening to obtain the strains of which the width of the bacteriostatic band is greater than 5mm; determining the bacteriostatic action of sterile fermented liquid on the four fruit postpartum pathogenic fungi by using an oxford cup method; and screening to obtain the bacillus amyloliquefaciens 1A of which the width of the bacteriostatic band is greater than 10mm. Therefore, the marine bacillus amyloliquefaciens 1A has a broad application prospect.

Methods and compositions comprising essential oils or components thereof for protecting harvested fruits against decay caused by a specific pathogenic fungus are provided, such as Cinnamon cassia oil for protection of persimmons against Alternaria alternata, strawberries against Botrytis cinerea, or mandarins against Penicillium italicum or Penicillium digitatum.

The invention relates to the technical field of microorganisms. The camphor tree endophytic fungi disclosed by the invention is separated from a camphor tree plant living body collected in the Tianmu Mountain in Hangzhou city of Zhejiang province through use of an endophytic fungus microorganism separation technology and is called after Alternaria alternata 31 through microbial taxonomic identification; and the strain is preserved in the General Microorganism Centre of China Microorganism Strain Preservation Management Committee on December the 31st, 2010 and has a preservation registered number of CGMCC No.4508. The strain liquid is obviously characterized by generating an active metabolic product which has the inhibiting effect on plant fungus disease pathogens, such as cucumber rhizoctonia solani, watermelon fusarium oxysporium and tomato botrytis cinerea, and the like through fermenting and is an important microbial resource in agriculture.

The invention discloses Bacillus velezensis 05-1205 as well as an acquisition method and application thereof, and belongs to the technical field of microbial application. The Bacillus velezensis 05-1205 is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number of the Bacillus velezensis 05-1205 is CCTCC No: M2020568. The acquisition method comprises processes of separating, screening and purifying, firstly, endophytic bacteria are seperated from healthy tobacco plants, endophytic bacteria antagonizing rhizopus oryzae are screened by taking the tobacco mildew pathogenic bacteria rhizopus oryzae in a baking period as an indicator, and finally, purifying is conducted on a culture medium plate to obtain the Bacillus velezensis 05-1205. The application ofthe bacterial strain comprises application in tobacco mildew control, the bacterial strain Bacillus velezensis 05-1205 can achieve effective control over tobacco mildew in the baking period, and therelative control effect is 70% or above. Secondly, the Bacillus velezensis 05-1205 is wide in antibacterial spectrum, and has a very good antibacterial effect on tobacco bacterial wilt bacteria, botrytis cinerea, sclerotinia sclerotiorum, target leaf spot bacteria, root rot bacteria and alternaria alternata besides pathogenic bacteria of tobacco mildew in the baking period.

The invention relates to a method for rapidly detecting alternaria tenuissima ketone acid. The method is based on a horse radish peroxidase catalysis luminol-hydrogen peroxide luminescence system, indirect competitive chemiluminescent enzyme-linked immunosorbent assay of the alternaria tenuissima ketone acid is built, and the method has the advantages of high sensitivity of a chemiluminescence and high specificity of an immunoassay. The detection range of the method is 0.032ng / mL-30.244ng / mL, IC50 (half maximal inhibitory concentration) is 0.973ng / mL, lowest detection limit is 0.010ng / mL, a linear regression equation is y=49.82-20.14x, and R20=0.9966. Compared with a traditional ELISA (enzyme-linked immunosorbent assay) method, the IC50 and the lowest detection limit are lower and high in specificity, duration time of light-emitting signals is long and can reach several hours, linear detection range is wider, and the method provides bases for research of limit standards of the alternaria tenuissima ketone acid, detection of pollution level of the alternaria tenuissima ketone acid and risk analysis.

The invention provides Trichoderma asperellum TD3104 and application thereof in preparation of microbial agent for inhibiting plant pathogenic bacteria. The preservation number of the Trichoderma asperellum TD3104 is CGMCC 13161. The invention further provides application of the Trichoderma asperellum in the controlling of the plant pathogenic bacteria of tobacco and apples. The antagonistic Trichoderma asperellum can effectively inhibit the growth of Phytophthora parasitica var. nicotianae, ralstonia solannacearum, rhizoctonia solani, tobacco root rot pathogenic fusarium, alternaria alternata and the pathogenic bacteria of apple rot and apple ring rot, can effectively control related diseases, can reduce the use amount of chemical pesticides and accumulation of the chemical pesticides in soil, is beneficial to the increasing of quality and yield of the economic crops of China and is evident in economic and social benefits.

The present invention relates to protein for improving disease resistance and enhancing plant growth, and the encoding gene. The present invention provides a type of protein PEAt1 separated from chain Alternaria. The protein has the function of improving disease resistance and enhancing plant growth. The present invention also relates to the preparation method and uses of the protein and multi nucleotide acid, and the expression carrier and transformer constructed by the gene of the multi nucleotide acid.

The invention provides biocontrol endophytic fungi- Alternaria alternata and relates to the technical field of microorganisms. The endophytic fungi of camphor trees are separated from camphor tree living bodies collected from Tianmu Mountain in Hangzhou, Zhejiang by an endophytic fungi separation technique and are named as Alternaria alternata 31 according to microbial taxonomy identification. The biocontrol endophytic fungi-Alternaria alternata is collected in the General Microbiology Center of China Microorganism Strain Collection Management Committee, wherein the collection date is 2010.12.31 and the collection registered number is CGMCC No.4508. The biocontrol endophytic fungi-Alternaria alternata has the most remarkable characteristic that: the strain liquid is fermented to generate active metabolites which can inhibit plant fungi disease pathogenic bacteria such as rhizoctonia solani, fusarium oxysporium, botrytis cinerea and the like and is important microbial resources agriculturally.

The invention discloses a method for screening an alternaria alternata resistant germplasm material from a petri dish. The method comprises the following steps of: a, culturing alternaria alternata on an agar culture medium; b, carrying out shaking culture of the alternaria alternata in a Czapek culture solution; c, coarsely extracting alternaria alternata toxin; d, determining activity of the toxin by using a biological assay method; e, adding water for diluting a toxin solution and preparing a culture medium; f, carrying out 121-DEG C moist heat sterilization on a toxin-containing culture medium for 15-30min; g, disinfecting tobacco seeds; h, culturing the tobacco seeds on the toxin-containing culture medium; and I, identifying the alternaria alternata resistant tobacco germplasm material according to the condition whether a tobacco shoot root grows in the culture medium. According to the invention, the toxin-containing culture medium is prepared by using the toxin generated by the alternaria alternata, the tobacco seeds are cultured on the culture medium, and the alternaria alternata resistant germplasm material is directly screened from the tobacco seeds rapidly, economically, conveniently and reliably. The method can replace field test screening and can be used for screening the alternaria alternata resistant germplasm material in the petri dish.

The invention relates to a preparation method of an anti-phytopathogen fermentation liquid of a spirea endophytic fungus. The spirea endophytic fungus is named Alternaria alternata XXB07 and was collected in China General Microbiological Culture Collection Center on March 8, 2013; and the collection number is CGMCC No.7292. The method comprises the following operation steps: 1. performing strain activation; 2. performing seed culture, and 3. performing fermentation culture to obtain a fermentation liquid of the spirea endophytic fungus, filtering the fermentation liquid under aseptic conditions to remove mycelia, and passing through a 0.22-micrometer filter to obtain the antimicrobial fermentation liquid of the spirea endophytic fungus. The invention confirms the preparation method of the antimicrobial fermentation liquid of the Alternaria alternata XXB07 strain having antibacterial activity; and antimicrobial tests of the antimicrobial fermentation liquid of the Alternaria alternata XXB07 discover that a fermentation liquid eluate and a mycelium extract of the strain have mycelium growth inhibition action on plant pathogenic fungi of Phytophthora capsici.

The invention discloses a pseudo-ginseng WRKY transcription factor gene PnWRKY9 and the application thereof. The nucleotide sequence of the PnWRKY9 gene is as shown in SEQ ID NO: 1, and the PnWRKY9 gene encodes a WRKY transcription factor; according to the application, molecular biology and functional genomics related technical researches prove that the PnWRKY9 gene has the function of improving the fungal disease resistance of plants, the antifungal gene PnWRKY9 is constructed on a plant expression vector and transferred into tobacco for overexpression, and the transgenic tobacco plant has very strong in-vitro antifungal activity; according to the present invention, the PnWRKY9 overexpressed transgenic tobacco has the significant inhibition effect on the growth of Alternaria Compacta, Fusarium solani, and Nigrospora oryzae, and the PnWRKY9 overexpressed transgenic tobacco has the significant inhibition effect on the growth of the Alternaria Compacta, the Fusarium solani, and the Nigrospora oryzae, such that the PnWRKY9 overexpressed transgenic tobacco has the significant inhibition effect on the growth of the Nigrospora oryzae,.

The invention discloses a pseudo-ginseng inducible promoter R1 and application thereof, the nucleotide sequence of R1 is shown as SEQ ID NO: 1, and molecular biology and genetic engineering related technical researches prove that the pseudo-ginseng promoter R1 responds to several plant hormones, biological stress and abiotic stress; a fusion expression cassette constructed by the panax notoginseng promoter R1 and the beta-glucuronidase gene is transferred into tobacco for expression, and the activity of the glucuronidase of the transgenic tobacco is quantitatively detected through a fluorescence method. The result shows that after the transgenic tobacco is treated by fusarium oxysporum, fusarium solani, alternaria alternata, NaCl, AlCl3, gibberellin, methyl jasmonate, salicylic acid, abscisic acid and ethephon, the activity of glucuronidase is obviously enhanced; the pseudo-ginseng promoter R1 is induced by several plant hormones and biological and abiotic stress factors, and can be used for plant disease-resistant gene engineering.

The invention provides an antagonistic strain 12a generating volatile bacteriostatic gas and application thereof in preventing and treating fruit-vegetable diseases. The antagonistic strain 12a is Bacillus vallismortis and is collected under CGMCC No.12179. The antagonistic strain 12a is obtained by collecting and separating peach tree rhizosphere soil from a Feicheng peach orchard without being applied with chemical fertilizer and pesticide, and the volatile bacteriostatic gas generated in fermentation liquid of the antagonistic strain 12a has strong antagonistic effect on Monilinia fructicola and also has obvious inhibiting effect on Marssonina mali caused by Alternaria mali, sweet potato black spot caused by Ceratocystis fimbriata Ellis.Et Halsted, citrus blue mold caused by Penicillium italicum Wehmer and winter jujube black spot caused by Alternaria alternata. The antagonistic strain 12a can effectively prevent and treat various fruit-vegetable diseases, is a biological preventing and treating potential strain which is good in biological preventing effect, pollution-free, wide in preventing and treating range, safe and ecological and has wide market application prospect.

This invention relates to a fungal resistance gene of red skinned pear, namely a polygalacturonase-inhibiting protein gene (PpPGIP), and application. The PpPGIP gene has a base sequence which is stated in a sequence-list SEQ ID; the length of the PpPGIP gene is 1,032bp, wherein 990bp is an open reading frame; and the PpPGIP gene codes polygalacturonase-inhibiting proteins containing 330 amino acids. Functional genomics related technology prove that the PpPGIP gene has the function of improving antifungal function of plants. The antifungal PpPGIP gene is constructed on a plant expression vector and is excessively expressed after being transferred into tobacco, the transgenic tobacco for expressing PpPGIP has strong in vitro antifungal activity and the obvious inhibition effect on a plurality of funguses like Aspergillus niger, Phomopsis funguses, Alternaria alternata, penicillium, and the like.

The invention relates to bacilli, in particular to a bacillus DY26-004 and application thereof to prevention and control of plant pathogenic fungi. A preservation number of the Bacillus sp.DY26-004 is CCTCC NO:M 2015406. The bacillus DY26-004 has a strong inhibiting effect on various plant pathogenic fungi such as fusaria, colletotrichum musae, paecilomyces variotii, sclerotinia scleotiorum, alternaria longipes, trichoderma viride, rhizoctonia solani, alternaria alternata, botrytis cinerea and colletotrichum gloeosporioides, and has good biological prevention and control effects in pot experiments for prevention and control of sclerotinia scleotiorum and fusarium graminearum, wherein control efficiencies are 72% and 65% respectively. The bacillus DY26-004 which is a nontoxic, non-pathogenic and environment-friendly bacillus is a useful resource for research and development of microbial pesticides and has a potential application prospect.

The invention discloses bacillus velezensis Z002 as well as an acquisition method and application thereof. The bacillus velezensis Z002 is collected in China Center for Type Culture Collection (CCTCC), and the collection number is CCTCC No: M 2020567. The acquisition method comprises separation, screening and purification processes. Endophytic bacteria are firstly separated from tobacco plant tissue and subjected to dark culture, the endophytic bacteria antagonizing the tobacco mildew pathogen rhizopus oryzae are screened out, and finally purification is carried out to obtain the bacillus velezensis Z002. The application of the bacillus velezensis Z002 comprises fermentation, inoculation and modulation processes. Firstly, strains are cultured to obtain a fermentation inoculant, the fermentation inoculant is sprayed to tobacco petioles through a sprayer, tobacco leaves are put into a curing barn, and the tobacco leaves are cured according to a normal curing process. The strain bacillusvelezensis Z002 can effectively control tobacco mildew in the curing period, and the relative control effect is 80% or above. Secondly, the bacillus velezensis Z002 also has very good bacteriostatic effects on Ralstonia pseudosolanacearum, Phylophthora nicotianae, Botrytis cinerea, Sclerotinia sclerotiorum, Thanatephorus cucumeris, Fusarium oxysporum, Alternaria alternata and the like. The bacillus velezensis Z002 has good control effect and higher application and popularization value.

The invention discloses a lilium regale inducible promoter PR4 and application thereof, the nucleotide sequence of the PR4 is shown as SEQ ID NO: 1, and the lilium regale inducible promoter PR4 is proved to respond to several plant hormones and biological stress through molecular biology and genetic engineering related technical research; and a fusion expression cassette constructed by the lilium regale promoter PR4 and a beta-glucuronidase gene is transferred into tobacco for expression, and the glucuronidase activity of transgenic tobacco is quantitatively detected through a fluorescence method. Results show that after the transgenic tobacco is treated by methyl jasmonate, salicylic acid, fusarium oxysporum, alternaria alternata and fusarium chlamydosporum, the activity of glucosidase is obviously enhanced; therefore, the lilium regale promoter PR4 is a promoter induced by plant hormones and biological stress factors and can be used for plant disease-resistant gene engineering.

The invention relates to a strain capable of producing alpha-L-rhamnosidase and a method for producing alpha-L-rhamnosidase by adopting the strain through fermentation, belonging to the technical field of food biology. The invention discloses the strain capable of producing alpha-L-rhamnosidase. The classification name of the strain is Alternaria alternata SK 37.002, is preserved in China Center for Type Culture Collection with preservation number of CCTCC NO:M2015309. A fermentation culture medium taking sucrose and naringoside as carbon sources is used for fermenting Alternaria alternata SK 37.002, namely CCTCC NO:M2015309 to obtain the strain containing alpha-L-rhamnosidase; and the strain is broken, and alpha-L-rhamnosidase is separated and purified. The produced alpha-L-rhamnosidase is safe and reliable, is a functional product with market potential, and can be widely applied to the industries of foods, medicines, biology and the like. The strain can be used for efficiently producing alpha-L-rhamnosidase, and is suitable for large-scaled production.

The invention discloses a method for preserving alternaria alternate, comprising: field typical brown spot is collected, and germ spores are separated by using a simple single-spore method; bacterial strain with strong pathogenicity can be screened by a detached leaf pendant-drop method, tobacco leaves can be inoculated by being sprayed with spore liquid of the bacterial strain with strong pathogenicity, the inoculated leaves are moisturized and maintained the humidity level for pothogenesy, and the brown spot is dried in the air or parched, and is then sealed and preserved in a refrigerator at 2-4 DEG C; wherein, both the typical brown spot and scab which is taken as the strain to be preserved at the low temperature are applicable. The method adopting host plants for preserving the germs can effectively maintain the pathogenicity of the germs. Fresh spores with stable heredity can be conveniently prepared by being matched with the simple single-spore isolation after the scab is moisturized. The germs can be preserved for at least five years, so that the hereditary stability and the consistency of pathogenicity of the germs can be effectively maintained.