29 results about "Alternaria alternata" patented technology

The present application relates to the technical field of biology, and in particular discloses a PCR detection primer pair of Alternaria porri, a target and application thereof.The present application provides a PCR detection primer pair of Alternaria porri, and the nucleotide sequences of the primer pair are shown as SEQ ID No.1-2.After PCR amplification of DNA of a to-be-tested sample is carried out by using the primer pair of the present application, and then whether specific bands appear or not is observed by combining with gel electrophoresis, it can be determined whether Alternaria porri is contained in the to-be-tested sample or not.The primer has high specificity and good sensitivity, can rapidly detect the presence of Alternaria porri in a sample, and can provide a reference for early inspection and quarantine and early prevention and treatment of Alternaria porri leaf spot.

The present application belongs to the technical field of fertilizer, and specifically discloses a foliar fertilizer for improving the disease resistance effect of Vietnamese ginseng, which is composed of 0.1-0.5% potassium dihydrogen phosphate, 0.1-0.2% boric acid, 0.3-0.7% urea, 0.005-0.02% chitosan, 0.0001% salicylic acid and 0.05% Tween-20, and the rest is water, according to the weight percentage. The foliar fertilizer prepared by the present application has a remarkable disease control effect on Vietnamese ginseng, and has a positive control effect on the infection of Fusarium oxysporum, Alternaria panax and Botryosphaeria dothidea. After using the foliar fertilizer of the present application, the growth of the pathogen is significantly inhibited from the phenotype, and the disease onset time of Vietnamese ginseng is significantly later than that of the control. The resistance gene expression of Vietnamese ginseng is significantly improved through testing.

The application discloses a tobacco endophytic fungus metabolite, a preparation method and application in tobacco alternaria alternata prevention and treatment, and relates to the technical field of microbial natural product pesticides. The tobacco endophytic fungus metabolite is a benzoquinone compound peniquinone L, and a structural formula is as follows: The benzoquinone compound peniquinone L is produced by fermentation of tobacco endophytic fungus Aspergillus aculeatus Aspergillus aculeatus Aa-1, and scale fermentation and preparation production are easy to carry out. The tobacco endophytic fungus metabolite benzoquinone compound peniquinone L of the application has inhibitory activity on various agricultural pathogens, and has significant bacteriostatic activity on tobacco alternaria alternata in particular, with an MIC value of 2 μg / mL, which is superior to positive drug carbendazim, and can be used as a leading compound or a new microbial natural product pesticide for tobacco alternaria alternata prevention and treatment.

The present application discloses a Streptomyces pseudovenezuelae and application of the Streptomyces pseudovenezuelae as a biocontrol agent in preventing and treating plant diseases. Streptomyces pseudogriseolus The Streptomyces pseudovenezuelae M5 screened by the present application is preserved in China Center for Type Culture Collection, and the preservation registration number is CCTCC NO: M 20252042. The Streptomyces pseudovenezuelae M5 screened by the present application has a strong inhibiting effect on a plurality of filamentous fungal pathogenic bacteria, has an obvious prevention and treatment effect on leek leaf rot disease caused by Alternaria alternata, and has an obvious effect on a newly discovered pathogenic bacterium Microdochium dimidiatum on blueberries. The M5 has a good biocontrol function and a growth promotion effect, has an excellent application prospect as a biocontrol agent for preventing and treating plant diseases, and especially provides an effective biocontrol preparation for diseases caused by the newly discovered pathogenic bacterium Microdochium dimidiatum on blueberries.

This invention belongs to the fields of microbial technology and veterinary pharmacology, and discloses a strain of *Alternaria alternata* isolated from Artemisia argyi, its fermentation supernatant, and its application in the preparation of anti-PRRSV drugs. The strain's preservation number is CCTCC NO: M2026443. The fermentation supernatant of this strain can inhibit PRRSV infection of cells and inhibit viral proliferation on cells. Cell-level anti-infection studies show that this strain has significant anti-PRRSV activity in vitro, reducing viral titer, viral genome copy number, and viral nucleoprotein production. It can be used to prepare a broad-spectrum anti-porcine reproductive and respiratory syndrome virus drug to control the occurrence of porcine reproductive and respiratory syndrome.

The application provides a strain for preventing and treating finger disease of bergamot Bacillus subtilis The application of B702 aims to solve the technical problem that the prevention method of finger disease of bergamot in the prior art is easy to leave residues and pollute the environment. The pathogenic bacteria causing the finger disease of bergamot is identified as Alternaria alternata through tissue separation, pathogenicity determination and morphological and molecular biological (rDNA-ITS and ATPase gene) identification. A bacterial strain with significant antagonistic effect is isolated and screened from the rhizosphere soil of healthy bergamot Bacillus subtilis The B702 is identified as Bacillus subtilis through 16S rRNA gene sequence analysis. The plate confrontation method determination result shows that the Bacillus subtilis strain Bacillus subtilis The average inhibition rate of the B702 to the pathogenic bacteria reaches 92.5% or more, the effect is stable and significant, the strain is a naturally existing plant rhizosphere beneficial bacteria, is safe to human and livestock, does not pollute the environment, provides a new resource for developing green and efficient biological pesticides, and has important significance for realizing the safe production of bergamot.

This invention discloses coumarin Schiff base derivatives. These derivatives are based on coumarin, and a series of coumarin derivatives with Schiff base structures are introduced through a reaction. These compounds exhibit good antifungal activity against *Botrytis cinerea*, *Alternaria alternata*, *Fusarium oxysporum*, and *Alternaria alternata*, opening up new avenues for the control of various plant fungal diseases and possessing broad development potential and promising application prospects. Furthermore, in vitro antioxidant experiments on these Schiff base compounds showed that they exhibit excellent scavenging abilities against DPPH radicals, ABTS radicals, and hydroxyl radicals, with antioxidant performance comparable to the positive control ascorbic acid, demonstrating their potential as novel antioxidants.

The present application relates to the culture method of Streptomyces variabilis TRM 45540, and the application thereof in broad-spectrum antibacterial agent, which can prevent and treat pepper anthracnose, cotton verticillium wilt, cotton fusarium wilt, alternaria alternate, pear rot disease, and orwinia solanacearum disease. Especially, the application thereof in pepper anthracnose prevention and treatment can inhibit the growth of pepper anthracnose pathogen, and realize the safe and efficient biological prevention and treatment of pepper anthracnose.

The application discloses application of a broad-spectrum antibacterial biocontrol Cytospora fungus F-XTBG8 in preparation of antifungal active substances. Chaetomella raphigera The application of the broad-spectrum antibacterial biocontrol Cytospora fungus (F-XTBG8) is applied in preparation of antifungal active substances. The strain has obvious antagonism to more than 100 environmental fungi and plant pathogenic fungi including Pyricularia oryzae (Monascus oryzae), Mycosphaerella fijiensis (Mycosphaerella fijiensis), Alternaria tenuissima (Alternaria tenuissima), Fusarium graminearum (Fusarium graminearum), Fusarium oxysporum (Fusarium oxysporum) and Fusarium verticillioides (Fusarium verticillioides), and fermentation liquor of the strain can significantly reduce occurrence of corn large spot, tobacco black spot and sclerotinia rot. Magnaporthe oryzae Setosphaeria turcica Alternaria tenuissima Fusarium graminearum Fusarium oxysporum Fusarium concentricum The application provides an excellent biocontrol strain for development and research of microbial source pesticides and provides a new idea for green prevention and control of plant diseases.​​​​​

This invention discloses a *Streptomyces xylose-lyticus* strain P6-2-1 antagonistic to multiple plant pathogenic fungi and its applications. *Streptomyces xylose-lyticus* P6-2-1 was deposited on November 27, 2025, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 36794, and is classified as *Streptomyces xylose-lyticus*. Streptomyces xylanilyticus The *Streptomyces xylose-degrading* P6-2-1 strain is effective against *Alternaria alternata*, *Fusarium solanum*, *Fusarium oxysporum* race 1 (Cuba specialized type), *Fusarium oxysporum* race 4 (Cuba specialized type), and *Fusarium graminearum*. 、 Verticillium dahliae exhibits inhibitory effects and a broad spectrum of inhibition. It can be effectively used to control plant diseases including apple rot, apple leaf spot, apple replant disease, banana wilt, cotton wilt, and corn stem rot, showing promising prospects for biocontrol applications.

This invention discloses a transcription factor gene of Minjiang lily. LrbHLH1 Its nucleotide sequence is as described in SEQ ID NO: 1, encoding a protein with the amino acid sequence shown in SEQ ID NO: 2. This invention has confirmed this through reverse genetics technology. LrbHLH1 The gene has the function of enhancing plant resistance to fungi, and the present invention will... LrbHLH1 Genes were constructed into plant expression vectors and overexpressed in tobacco. The transgenic tobacco plants exhibited strong resistance to fungal infection, demonstrating the effectiveness of overexpression. LrbHLH1 The genetically modified tobacco plants exhibit high levels of resistance to infection by *Alternaria alternata* and *Alternaria solani*.

This invention belongs to the field of microbiology, specifically relating to a method for the biosynthesis of Alternaria alternata compounds. This method involves co-culturing Alternaria alternata and Ulva nigricans, followed by extraction with ethyl acetate to obtain high-yield Alternaria alternata compounds. This biosynthetic method is green, environmentally friendly, and sustainable, effectively increasing the biosynthetic yield of Alternaria alternata compounds and providing a new pathway for the research and industrial development of these compounds, showing great promise.

This invention discloses a *Trichoderma echinococcus* strain BBR-A and its application in the control of fungal diseases in blueberries, belonging to the field of biological control technology for blueberry fungal diseases. A *Trichoderma echinococcus* strain BBR-A was isolated from the rhizosphere soil of healthy blueberries and deposited at the China Center for Type Culture Collection (CCTCC) on June 4, 2025, with accession number CCTCC NO: M20251239. The *Trichoderma echinococcus* strain BBR-A of this invention significantly inhibits *Botrytis cinerea*, *Alternaria alternata*, and *Fusarium oxysporum*. Microscopic and electron microscopic examination revealed that *Trichoderma echinococcus* hyphae exhibited hyperparasitic phenomena such as entanglement, penetration, and dissolution upon contact with the hyphae of these three pathogens. The incidence of disease in blueberry roots and leaves treated with *Trichoderma echinococcus* was significantly reduced. These experimental results demonstrate that *Trichoderma echinococcus* BBR-A has a significant inhibitory effect on the growth and pathogenicity of blueberry fungal pathogens and can be used as a biological control agent for the control of blueberry fungal diseases, showing good application prospects.

The present application relates to the technical field of microbial culture and biological control, and particularly relates to a method for inducing high-efficiency synthesis of antibacterial lipopeptide by bacillus, wherein the high-efficiency synthesis of antibacterial lipopeptide is promoted by co-culture induction through always contacting metabolites of pathogenic fungi during the culture of bacillus; the bacillus is bacillus velezensis EM-1, and the preservation number is CGMCC No.21131; the pathogenic fungi is alternaria alternata XN01, and the preservation number is CGMCC No.42504. Through the physical isolation of bacillus velezensis EM-1 and alternaria alternata XN01, pure chemical communication between microorganisms is realized, the synthesis amount of lipopeptide antibacterial substances such as fengycin in bacillus velezensis EM-1 is significantly improved, and a new way for developing efficient and stable microbial biocontrol agents is provided.

This invention discloses a class of imidazo[1,2-]azoline containing a hydrazone structure. a Preparation methods and applications of pyridine derivatives, this invention uses imidazo[1,2- a Using the pyridine skeleton as the parent structure, a series of imidazo[1,2-] hydrazone fragments were introduced to design and synthesize a series of imidazo[1,2-] hydrazone-containing structures. a Pyridine derivatives. In vitro activity results showed that these compounds had excellent inhibitory activity against plant pathogenic fungi (such as *Botrytis cinerea*, *Colletotrichum gloeosporioides*, *Colletotrichum sorghum*, *Colletotrichum tea*, *Alternaria alternata*, and *Rhizoctonia solani*), and some of the target compounds also showed good inhibitory activity against plant pathogenic bacteria.

The application discloses a Trichoderma guepinii GUDJ16 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the Trichoderma guepinii GUDJ16 screened by the application is CCTCC NO: M 20252004. The Trichoderma guepinii GUDJ16 has a prevention and treatment effect on kiwi soft rot, and the inhibition rate of the Trichoderma guepinii GUDJ16 on Botryosphaeria dothidea can reach 77.15%. The Trichoderma guepinii GUDJ16 also has an inhibition effect on Fusarium solani, Fusarium graminearum, Fusarium asiaticum, Rhizoctonia solani, Sclerotium rolfsii, Colletotrichum musae, Passalora fulva and Alternaria tenuissima. It is found through disease prevention and treatment effect verification on in-vitro fruits infected with kiwi soft rot that the prevention and treatment effect of the Trichoderma guepinii GUDJ16 on kiwi soft rot can reach 61.26% after treatment, and therefore the Trichoderma guepinii GUDJ16 has high application value in the field of kiwi soft rot prevention and treatment.

This invention relates to the field of cultural relic protection technology, specifically to a plant-based antibacterial agent for the prevention and control of microorganisms in earthen sites and its screening method. The antibacterial agent uses citral as its core component and ensures high antibacterial efficiency through a systematic screening method (including microbial identification, inhibition zone testing, MIC / MBC determination, and persistence assessment). Experiments show that citral has strong inhibitory effects on common pathogens in earthen sites (such as Alternaria and Mucor), and a 6% concentration can achieve at least 7 days of sustained antibacterial activity. This invention is green and safe, providing a reliable solution for the prevention and control of microorganisms in earthen sites.

The application discloses pyridine coumarin derivatives, a preparation method and application thereof. The pyridine coumarin derivative is selected from the following structural general formula: the pyridine coumarin derivative is independently designed and synthesized, and the experimental results show that the pyridine coumarin derivative has good antifungal activity on botrytis cinerea, alternaria solani, fusarium oxysporum and alternaria alternata, which expands a new field for prevention and treatment of various plant fungal diseases, has wide development space and good development and application prospect, and has good application value.

The present application relates to the technical field of medicine, in particular to an extraction and separation method and application of an anti-inflammatory active compound in Leucophyllum angustifolium. Seven compounds are separated and identified from Leucophyllum angustifolium by a specific extraction and separation method, the separated and identified compounds are classified in the first level, there are six benzyl alcohol compounds, which are phellodenol-A (1), chengzhong wintergreen (2), 2,4-dimethoxy cinnamic acid (4), alternaria alternata phenol (5), alternaria alternata phenol monomethyl ether (6) and alternaria alternata phenol-3,9-dimethyl ether (7); there is one alkaloid compound, which is new sea urchin spirit A (3). Among them, four compounds are separated from the plant of Leucophyllum for the first time, which are phellodenol-A (1), new sea urchin spirit A (3), 2,4-dimethoxy cinnamic acid (4) and alternaria alternata phenol-3,9-dimethyl ether (7).

This invention discloses a class of acetylhydrazine derivatives with phenyl heteroatom substituents, their preparation methods, and applications; the acetylhydrazine derivatives have the general structural formula shown below, and exhibit excellent broad-spectrum antifungal effects against fungi including *Fusarium graminearum*, *Sclerotinia sclerotiorum*, *Botrytis cinerea*, *Rhizoctonia solani*, *Alternaria alternata*, *Curvularia lunata*, *Phytophthora capsici*, *Sclerotium sclerotiorum*, and *Fusarium graminearum*.

This invention relates to the field of tobacco processing technology and discloses a method for fermenting tobacco sheets based on biomarker-triggered staged fermentation. The method specifically involves spraying a compound microbial agent containing *Alternaria alternata*, *Kluyveromyces martensii*, and *Lactobacillus plantarum* onto the surface of tobacco sheets, followed by staged fermentation based on biomarkers. Specifically, when the concentration of reducing sugar in the tobacco sheets is less than 2 mg / g, fermentation continues at an initial temperature of 36-40°C; when the concentration of reducing sugar is above 2 mg / g and the pH is greater than 5.0, the fermentation temperature is switched to 27-32°C; and when the concentration of reducing sugar is above 2 mg / g and the pH is below 5.0, the fermentation temperature is switched to 20-25°C. This invention, based on a specific compound microbial agent, uses reducing sugar and pH as biomarkers to trigger stage switching, achieving precise matching of the metabolic characteristics of the compound microorganisms and significantly increasing the content of aroma-producing substances such as esters.

The application belongs to the field of agricultural microorganism technology, and discloses bacillus velezensis Bac8 and application thereof in prevention and treatment of black spot disease of cabbage. The strain is isolated from soil of a cabbage planting field, and has been preserved in China Center for Type Culture Collection on February 28, 2026, with a preservation number of CCTCC NO: M 2026354. The strain has significant antagonistic effect on alternaria brassicae, and can be used for development of a biocontrol product, and has a good application prospect in prevention and treatment of black spot disease of cabbage caused by alternaria brassicae.

The application discloses active polysaccharide, an extraction reagent and method thereof and application of the active polysaccharide. 4 The active polysaccharide has a weight average molecular weight of 7.04*10 4 The application uses seaweed gel extraction waste liquid as raw material, and uses ethanol, polyhydric alcohol and quaternary ammonium base to prepare the extraction reagent, separates crude polysaccharide in a short time, removes inorganic substances, proteins and color, and carries out gel column chromatography, so that the purity of the polysaccharide is improved (up to 92.26 %); and the activity of resisting plant pathogenic fungi is well preserved through secondary freeze-drying; the minimum inhibitory concentration of the polysaccharide to alternaria alternata and alternaria alternata is 0.01 mg / mL, and the inhibition rate is 81.98 % and 85.47 % respectively at 0.16 mg / mL. The whole extraction method is simple and environment-friendly, the process condition is mild, and the high-value utilization of the seaweed gel extraction waste liquid is realized.

The application provides application of taurine in promoting growth of Panax notoginseng, inhibiting bacteria, inducing Panax notoginseng to produce resistance and / or relieving Panax notoginseng continuous cropping obstacles, and further provides a method for promoting growth of Panax notoginseng, inhibiting bacteria, inducing Panax notoginseng to produce resistance and / or relieving Panax notoginseng continuous cropping obstacles, and a biological pesticide, a Panax notoginseng resistance inducer, a Panax notoginseng growth regulator and a Panax notoginseng continuous cropping obstacle reliever. Research shows that within a certain concentration range, taurine can significantly promote the growth of Panax notoginseng, inhibit the growth of the root rot pathogen of Panax notoginseng, reduce the occurrence of root rot, significantly relieve the continuous cropping obstacles of Panax notoginseng and enhance the resistance of Panax notoginseng to Alternaria alternata, and significantly reduce the disease area of Alternaria alternata. Moreover, taurine has the advantages of low toxicity, no pollution, environmental friendliness and the like, and is an effective way for green ecological prevention and control of Panax notoginseng.

This invention discloses a polyaspartic acid-coated cubic mesoporous silica nanoparticle drug-carrying system, its preparation method, and its applications, relating to the field of novel nanoparticle pesticide formulations. The polyaspartic acid-coated cubic mesoporous silica nanoparticle drug-carrying system of this invention has a core-shell structure, with a core of cubic mesoporous silica nanoparticles and an outer shell of polyaspartic acid coating the surface of the mesoporous silica nanoparticles. The nanoparticle drug-carrying system of this invention has a cubic structure, a drug loading capacity of 22.8%, and pH responsiveness. Simultaneously, the encapsulation with polyaspartic acid gives the nanoparticle drug-carrying system good leaf adhesion properties, reducing pesticide droplet slippage loss. In terms of bioactivity, this drug-carrying system exhibits stronger antibacterial activity against Alternaria alternata and Botrytis cinerea. Biosafety assessments show that it not only effectively promotes wheat seedling growth but also significantly reduces toxicity to zebrafish.

This invention relates to the field of biocontrol and growth-promoting microbial technology, specifically disclosing a strain of *Basilus asiatica* G19 with antagonistic fungal activity and growth-promoting functions, and its applications. *Basilus asiatica* G19 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 42329. This strain exhibits antagonistic activity against various plant pathogenic fungi, including *Alternaria alternata* (apple-specific type), *Alternaria maculata*, *Fusarium solani*, *Fusarium graminearum*, *Fusarium oxysporum* race 1 (Cuba-specific type), and tropical race 4; simultaneously, it significantly promotes maize growth, increasing plant height, stem diameter, and fresh weight of both above-ground and below-ground parts. The *Basilus asiatica* G19 provided by this invention has significant application value in plant disease control and crop growth promotion and yield increase.

The application discloses an indoor high-efficiency identification method of Chinese cabbage black spot induced by Leptosphaeria maculans, and comprises the following steps: S1, Chinese cabbage seedling culture; S2, preparation of spore suspension; S3, inoculation of test materials; and S4, culture and investigation of the test materials. Compared with the prior art, the application has the advantages of significantly shortened identification period, realized high-efficiency screening, high controllability of environment, high repeatability of results, reduced risk of misjudgment, saved resource cost and consistent green and sustainable agricultural development direction.

The application discloses streptomyces virginiae M54 and application thereof in preventing and treating plant diseases. Streptomyces virginiae The application discloses streptomyces virginiae M54 and application thereof in preventing and treating plant diseases. The application discloses streptomyces virginiae M54 and application thereof in preventing and treating plant diseases. The application discloses streptomyces virginiae M54 and application thereof in preventing and treating plant diseases. The application discloses streptomyces virginiae M54 and application thereof in preventing and treating plant diseases. The application discloses streptomyces virginiae M54 and application thereof in preventing and treating plant diseases.