254 results about "Alternaria alternata" patented technology

The invention relates to the field of microorganisms, in particular to bacillus subtilis QNF2 and application of the bacillus subtilis QNF2 to prevention and control of fruit and vegetable diseases. The bacillus subtilis QNF2 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center on August 26, 2024, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.31740. The bacillus subtilis QNF2 disclosed by the invention has the advantages that the bacillus subtilis QNF2 disclosed by the invention can be used for preparing the bacillus subtilis QNF2; according to the present invention, with the application of the bacillus subtilis QNF2, the growth of pathogenic bacteria such as Monilinia fucicola, Botryosphaeria dothidea, Alternaria alternata, Fusarium oxysporum, Aspergillus niger, Penicillium digitatum, Botrytis cinerea and the like can be significantly inhibited, and the occurrence of related fruit and vegetable diseases can be efficiently prevented and controlled, for example, the prevention and control effect of the volatile matter VOCs of the bacillus subtilis QNF2 on the peach brown rot caused by the Monilinia fucicola can be up to 98%; and the fruit quality, especially the fruit hardness, can be improved, and an important role in prolonging the storage period of the fruits is played.

The invention discloses a Trichoderma auritum GUWM22 strain and application thereof, and belongs to the field of microorganisms. The preservation number of the trichoderma auritum GUWM22 is CCTCC (China Center for Type Culture Collection) NO: M20242639, the preservation date is November 26, 2024, the preservation unit is China Center for Type Culture Collection, and the preservation address is Wuhan University, Wuhan, China. Experiments prove that the GUWM22 has a very strong inhibition effect on six pathogenic bacteria (phytophthora nicotianae, phytophthora capsici, botryosphaeria dothidea, alternaria alternata, phomopsis and colletotrichum capsici), the bacteriostasis rate reaches 78% or above, in addition, the GUWM22 also has a good prevention and treatment effect on anthracnose of picked peppers (line peppers), the prevention effect is 82.11%, and it can be seen that the GUWM22 can be used for preventing and treating anthracnose of the picked peppers (line peppers). The Trichoderma aureum GUWM22 is a new biocontrol Trichoderma strain with good potential. The invention provides a new strain resource and an environment-friendly and efficient biocontrol approach for effective prevention and treatment of pepper anthracnose.

The invention discloses lysinibacillus sp. For degrading alternaria alternata toxins and application of the lysinibacillus sp., and belongs to the technical field of microorganisms, the lysinibacillus sp. Is preserved in China Center for Type Microbiological Culture Collection, the preservation address is Wuhan University, Hubei Province, and the preservation number is CCTCC No.M20241816. The invention further discloses a preparation method of the lysinibacillus sp. According to the invention, the Lysinibacillus macroides capable of effectively reducing and degrading alternaria alternata toxin is obtained for the first time, and the quality of agricultural products (including aquatic products) is favorably improved.

The invention discloses a Brucella with antibacterial activity and application of the Brucella. The invention discloses Burkholderia sp.HNIV0701, and the preservation number of the Burkholderia sp.HNIV0701 is GDMCC No: 66007. The Burkholderia sp.HNIV0701 disclosed by the invention has a stable, efficient and broad-spectrum antibacterial property, has an inhibition effect on a plurality of plant pathogenic bacteria such as Botryosphaeria dothidea, Phytophthora nicotianae, Fusarium solani, Alternaria alternata, Thanatepora cucurbitae, Ralstonia solanacearum and the like, and also can be used for inhibiting Staphylococcus aureus and / or Listeria monocytogenes, so that the Burkholderia sp.HNIV0701 can be used for inhibiting a plurality of plant pathogenic bacteria such as the Botryosphaeria dothidea, the Phytophthora nicotianae, the Fusarium solanum, the Alternaria solanacearum and the like. The Brucella HNIV0701 can hydrolyze organic phosphorus and can be used for soil improvement. The Brucella HNIV0701 has nitrogen fixation capability and has the potential of promoting plant growth.

The invention discloses an African trichoderma harzianum strain 6-5 and application thereof, the disclosed strain is named as Trichoderma harzianum, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. 41930. The bacterial strain has obvious bacteriostatic activity on crop pathogenic bacteria such as colletotrichum capsici, apple cyst, botrytis cinerea, alternaria longibrachiata and phytophthora capsici, and fermentation liquor of the bacterial strain has good prevention and control effects on apple anthracnose leaf blight, apple bitter rot and apple tree canker.

The present invention discloses a strain of Trichoderma wumengense GUWM22 and its application, which belong to the field of microorganisms. The deposit number of the Trichoderma wumengense GUWM22 is CCTCC NO: M20242639, the deposit date is November 26, 2024, the deposit unit is the China Center for Type Culture Collection, and the deposit address is Wuhan University, Wuhan, China. It has been verified that the GUWM22 bacteria have a strong inhibitory effect on 6 pathogens (Phytophthora nicotianae, Phytophthora capsici, Botrytis cinerea, Alternaria alternata, Phomopsis simiana and Echinops planifolia), and the inhibition rate is more than 78%. In addition, GUWM22 also has a good prevention and control effect on post-harvest anthracnose of pepper (strip pepper), with a prevention effect of 82.11%. It can be seen that Trichoderma wumengense GUWM22 is a new species of biocontrol Trichoderma with good potential. The present invention provides a new strain resource and an environmentally friendly and efficient biocontrol approach for the effective prevention and control of pepper anthracnose.

The invention discloses bacillus tequilensis P61 and application thereof in prevention and treatment of kiwi fruit soft rot, and belongs to the field of biological prevention and treatment. The bacillus tequilensis P61 provided by the invention is preserved in the China Center for Type Culture Collection on September 5, 2024, and the preservation number of the bacillus tequilensis P61 is CCTCC (China Center for Type Culture Collection) NO: M 20241916. According to the invention, the lipopeptide antibacterial substance is prepared according to the bacillus tequilensis P61. Experimental results show that the bacillus tequilensis P61 and lipopeptide antibacterial substances have an outstanding prevention and treatment effect on the soft rot of the kiwi fruits, have a remarkable bacteriostasis effect on pathogenic bacteria of the soft rot of the kiwi fruits such as botryosphaeria dothidea, alternaria alternata and phomopsis, remarkably reduce the damage rate of the kiwi fruits, are green and safe, are harmless to people and livestock, and are worthy of popularization and application. No pollution is caused to the environment. The invention provides a new biocontrol strain for biological prevention and control of kiwi fruit diseases, and has a wide application prospect.

The invention discloses a strain of disease-resistant and growth-promoting pseudomonas avenae LC40 and application thereof, and relates to the technical field of microorganisms, the pseudomonas avenae is specifically pseudomonas avenae LC40, the pseudomonas avenae LC40 is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 2, 2024, and the preservation number is CGMCC No.32869. The invention further discloses a method for preparing the disease-resistant and growth-promoting pseudomonas avenae LC40. The bacterial strain can be used for inhibiting tobacco mosaic virus (TMV), tomato brown fruit ruffle virus (ToBRFV), rice blast germ (Magnaporthe oryzae) and alternaria tenuissima (Alternaria tenuissima), and the bacterial strain can be used for inhibiting the tobacco mosaic virus (TMV), the tomato brown fruit ruffle virus (ToBRFV), the rice blast germ (Magnaporthe oryzae (Magnaporthe oryzae (Magnaporthe oryzae (Magnaporthe oryzae) and the allium tenuissima (Alternaria tenuissima). The Pseudomonas Asian LC40 provided by the invention has phosphorus solubilizing capacity and can secrete auxin compounds IAA, and field test results show that the LC40 strain has the effects of promoting growth and increasing yield of tomatoes. The pseudomonas Asian LC40 strain can effectively prevent and control plant diseases and promote crop growth, so that the yield and quality of crops are improved, and the pseudomonas Asian LC40 strain shows good application prospects of disease prevention and growth promotion.

The invention belongs to the field of microorganisms, and particularly relates to a banana endophyte compound fungicide as well as a preparation method and application thereof. According to the invention, a novel endophytic bacillus velezensis JDB15 strain and an endophytic trichoderma harzianum JDL4 strain are separated from a banana plant. The trichoderma harzianum JDL4 can inhibit botrytis cinerea, fusarium graminearum, rhizoctonia solani, peach anthracnose pathogen, peach brown rot pathogen and alternaria alternata, and has a good broad-spectrum antibacterial effect. Meanwhile, after the filtrate of the fermentation liquor of the trichoderma harzianum JDL4 and the bacillus velezensis JDB15 are compounded, the antibacterial effect is remarkably improved, and the control effect on banana fusarium wilt, pyricularia oryzae, tomato fusarium wilt and corn bipolaris maydis is also remarkably improved. The compound bacterial agent has wide biocontrol bacterial activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other plant diseases.

The bacillus velezensis MY-8 disclosed by the invention has obvious broad-spectrum antibacterial activity, and has an obvious inhibition effect on various plant pathogenic bacteria including cabbage alternaria alternata, rhizopus arrhizus, cladosporium very fine, fusarium tripteroides, alternaria alternata, Columbia cladosporium, botryosphaeria dothidea, fusarium oxysporum, geotrichum candidum, colletotrichum gloeosporioides and A. arundinis, and the bacillus velezensis MY-8 has an obvious inhibition effect on various plant pathogenic bacteria such as bacillus velezensis MY-8. The compound has a remarkable control effect on downy mildew, is expected to be prepared into a microbial preparation for biological control, and can be used as a biopesticide, a bio-fertilizer, a plant growth promoter and the like. The bacillus velezensis MY-8 disclosed by the invention can also express a structural domain containing protein of DUF4393, so that the bacillus velezensis MY-8 has potential bacteriophage infection resistance.

The invention belongs to the technical field of microorganisms, and particularly relates to application of utilizing alternaria alternata to ferment pericarpium citri reticulatae so as to improve the content of total flavonoids and hesperidin in pericarpium citri reticulatae, which comprises the following steps: culturing alternaria alternata spore suspension in a PDB liquid culture solution to obtain alternaria alternata inoculation liquid; uniformly mixing the sterilized pericarpium citri reticulatae powder and the sterilized rice powder, adding the alternaria alternata inoculation liquid, fully and uniformly mixing, and then adding sterilized water; after uniform stirring, the mixture is placed in a constant-temperature incubator to be fermented and co-cultured, the fermentation temperature is 25-35 DEG C, and the fermentation time is 5-12 days. According to the method disclosed by the invention, the efficient capability of the alternaria alternata in the aspect of converting flavonoid components in the pericarpium citri reticulatae can be fully exerted, so that the content of total flavonoids and hesperidin in the pericarpium citri reticulatae is remarkably increased.

The invention belongs to the technical field of plant disease prevention and treatment, and particularly relates to a biocontrol bacterium for preventing and treating idesia anthracnose, fermentation liquor, a biocontrol bacterium agent and application. According to the invention, a biocontrol bacterium is separated and screened from microbial resources on healthy idesia leaves, is classified and named as Bacillus velezensis, is preserved in China General Microbiological Culture Collection Center on March 28, 2025, and has a preservation number of CGMCC No.34038; the biocontrol bacterium provided by the invention has a good antagonistic effect on colletotrichum causing idesia anthracnose, melanospora causing idesia leaf spot, botrytis cinerea, fusarium oxysporum, alternaria alternata and pestalotiopsis which can cause plant diseases, and also has a good antagonistic effect on colletotrichum causing idesia anthracnose, melanospora causing idesia leaf spot, fusarium oxysporum, alternaria alternata and pestalotiopsis. The biocontrol bacterium, the fermentation broth and the biocontrol inoculant provided by the invention have potential application prospects in prevention and treatment of idesia anthracnose.

The invention discloses a colorectal cancer plasma circulating microbial marker, a reagent, a kit and application thereof, and belongs to the technical field of biomedical detection. The plasma circulating microbial marker for colorectal cancer comprises alternaria alternata, alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous The colorectal cancer plasma circulating microbial marker can be used for early screening of colorectal cancer, has relatively high sensitivity and specificity, and remarkably improves the accuracy and reliability of detection; the limitation of traditional ctDNA detection is broken through, and the detection rate of early colorectal cancer is improved. The colorectal cancer plasma circulating microbial marker in a colorectal cancer plasma sample is detected through low-depth whole genome sequencing, the requirement for depth in an experiment is reduced, the cost can be greatly reduced while high sensitivity and specificity are guaranteed, and the method is more suitable for large-scale screening.

The invention discloses trichoderma Guizhou GZMT12 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the trichoderma Guizhou GZMT12 screened by the invention is CGMCC (China General Microbiological Culture Collection Center) NO. 42152. According to the present invention, the bacterial strain can provide inhibition effects on seven pathogenic bacteria such as Rhodococcus solani Kuhn, Botryosphaeria dothidea, Alternania alternania, Phomopsis litsea, Colletotrichum truncatum, Phytotrichum capsici, and Phytotrichum nitianae, and further has advantages of good prevention and treatment effect, wide antibacterial spectrum, wide application range, and the like.

The invention relates to the technical field of microorganisms, in particular to fusarium and application thereof in promoting blueberry growth. The fusarium is classified and named as Fusarium sp., the strain number of the fusarium is NX1, the fusarium is preserved in the China General Microbiological Culture Collection Center on June 26, 2024, and the preservation number of the fusarium is CGMCC No. 41384. The strain has the capability of obviously promoting the growth of blueberries, and can be cooperated with Alternaria alternata sp.CS9 to further promote the growth of blueberry seedlings, so that an ideal strain resource is provided for developing efficient biological fertilizers or microbial preparations.

The invention belongs to the field of microorganisms, and particularly relates to bacillus velezensis with high stress resistance, bacterial powder, a biocontrol microbial inoculum and preparation and application thereof. The bacillus velezensis is bacillus velezensis Shannan.BV80-12 and is preserved in the China Center for Type Culture Collection, and the preservation number of the bacillus velezensis is CCTCC (China Center for Type Culture Collection) NO: M 2024386. The bacillus velezensis Shannan.BV80-12 provided by the invention has relatively strong tolerance to drought, and has the capability of producing indoleacetic acid (IAA). Meanwhile, the bacterial strain also has a broad-spectrum antibacterial ability, and has a relatively strong inhibition effect on fusarium graminearum, alternaria alternata, magnaporthe oryzae, bipolaris maydis, exserohilum turcicum, sclerotinia sclerotiorum, physalospora piricola, alternaria solani and citrus brown rot pathogen.

The invention discloses a lactobacillus casei strain capable of inhibiting activity of Chromarospora sp. And application of the lactobacillus casei strain in green pepper storage. The invention provides lactobacillus casei GHY-4, and the preservation registration number of the lactobacillus casei GHY-4 is CGMCC (China General Microbiological Culture Collection Center) No.31925. Furthermore, the invention provides a microbial agent of the lactobacillus casei GHY-4. The invention also protects the application of the lactobacillus casei GHY-4 or the microbial inoculum in inhibition of alternaria alternata. The invention also protects the application of the lactobacillus casei GHY-4 or the microbial inoculum in fresh keeping of green peppers. The method can be used for fresh keeping of the green peppers and has application and popularization values for the green pepper industry.

The invention relates to the field of microorganisms, in particular to a broad-spectrum antagonistic bacterium bacillus velezensis SJD33 and application thereof. The preservation number of the bacillus velezensis SJD33 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.29456. The bacillus velezensis SJD33 provided by the invention has the advantages that the preservation number is CGMCC No.29456; the strain has a remarkable inhibition effect on pear fruit rot pathogens (physalospora piricola, penicillium, alternaria alternata, athelia fruit rot pathogens, botrytis cinerea and colletotrichum gloeosporioides) and other fruit pathogenic bacteria (apple core pathogens, brown rot pathogens and pomegranate dry rot pathogens). The SJD33 can obviously reduce the natural rotting rate of the pear fruits; the SJD33 can be rapidly colonized in fruit wounds; the SJD33 does not influence the fruit quality and can delay the reduction of the fruit hardness and the L-ascorbic acid; the SJD33 secondary metabolite is stable in activity and has great application potential. The strain provides a new strain resource for a fruit disease biocontrol resource library.

The invention discloses an efficient broad-spectrum biocontrol bacterium pseudomonas FM36 and application thereof. The wheat pseudomonas is a strain FM36, is classified and named as Pseudomonas siliginis, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has a preservation number of CGMCC NO.32868. The invention further discloses a preparation method of the high-efficiency broad-spectrum biocontrol bacterium pseudomonas FM36. The invention further discloses an application of the high-efficiency broad-spectrum biocontrol bacterium pseudomonas FM36, and the application of the high-efficiency broad-spectrum biocontrol bacterium pseudomonas FM36. The invention further provides application of the wheat pseudomonas FM36 strain in promoting tomato growth and preventing and treating plant viruses and pathogenic fungi. The wheat pseudomonas provided by the invention has a promoting effect on the growth and yield increase of tomatoes, and has obvious antagonism and obvious antagonism on pathogenic fungi such as tobacco mosaic virus (TMV), chilli vein mottle virus (ChiVMV), rhizoctonia solani, Magnaporthe oryzae, alternaria tenuissima, colletotrichum sp.sp.cubense, fusarium oxysporum f.sp.cubense and the like, and the wheat pseudomonas has an obvious antagonism effect on pathogenic fungi such as the tobacco mosaic virus (TMV), the chilli vein mottle virus (ChiVMV), the rhizoctonia solani, the Magnaporthe oryzae, the alternaria tenuissima, the colletotrichum sp.sp.cubense and the like.

The invention discloses a litchi disease-resistant gene LcEXP1 and an application thereof. The litchi disease-resistant gene LcEXP1 is separated from litchis, the full-length cDNA (complementary deoxyribonucleic acid) of the gene is 750bp, and the encoded protein is an expansion protein with the full length consisting of 250 amino acids. The litchi disease resistance related gene LcEXP1 is transiently expressed in tobacco, different tobacco leaves are inoculated with phytophthora capsici and alternaria alternata after two days, it can be observed that after expression of the LcEXP1, scabs are remarkably reduced compared with negative control, and biostatistical analysis shows that the areas of the two treated scabs are remarkably different. Therefore, the gene can effectively promote the disease resistance of plants such as tobacco and the like, and has a wide application prospect in developing products for improving the disease resistance of the plants.

The invention discloses a strawberry and grape fresh-keeping composite coating film, a preparation method and application, the fresh-keeping composite coating film mainly comprises chitosan, oxidized starch, phytic acid and carvacrol, the rotting rate of strawberries and grapes is reduced, the chromaticity and hardness of the strawberries and the grapes are maintained, the aging of the strawberries and the grapes is delayed, and the fresh-keeping effect of the strawberries and the grapes is improved. The growth of rot-causing fungi alternaria alternata and Penicillium Orsenii in strawberries and grapes is inhibited, the quality of strawberry and grape fruits can be maintained, and the storage period of the strawberries and the grapes can be prolonged.

The invention discloses bacillus subtilis S1703 and application thereof, and belongs to the technical field of plant disease and pest control. The name of the bacterial strain is bacillus subtilis S1703, and the preservation number of the bacterial strain is GDMCC No: 66890. The bacterial strain S1703 has a wider antibacterial spectrum, and can effectively inhibit the growth of pathogenic bacteria such as fusarium oxysporum, colletotrichum gloeosporioides, alternaria alternata, aspergillus, phyllosticta, citrus reticulata, fusarium and the like; fermentation liquor of the S1703 has a good antibacterial effect on the psidium guajava wilt pathogen and the psidium guajava branch blight pathogen; the bacteriostasis rate on sigatoka sigatoka and guava leaf spot germs reaches 95% or above, and an excellent bacteriostasis effect is achieved. The strain S1703 can avoid potential adverse effects of chemical pesticides on the environment and crop safety, and has potential commercial development and application values in biological prevention and control practice of diseases.

The application discloses a strain of Mei Ji Maydomyces for delaying early leaf fall of pears and application thereof, and belongs to the technical field of microorganisms. The strain has a preservation number of CGMCC NO.31969 and a preservation date of September 13, 2024. The Mei Ji Maydomyces provided by the application is separated from the surface of healthy pear fruits, is harmless to human bodies, is high in safety, has strong bacteriostatic activity, and can effectively inhibit the growth of early leaf fall pathogenic bacteria of pear, such as Guibao anthracnose, Alternaria alternata and Neofusicoccum parvum. The application makes up for the deficiency of existing plant growth regulators and widens the application range of plant senescence delaying agents.

The invention discloses a pH and temperature dual stimulation response controlled release curcumin nano-pesticide and a preparation method and application thereof.The nano-pesticide comprises a carrier and curcumin loaded on the carrier, the carrier is N-isopropylacrylamide modified mesoporous silica, and the curcumin is a mixture of N-isopropylacrylamide modified mesoporous silica and N-isopropylacrylamide modified mesoporous silica and N-isopropylacrylamide modified mesoporous silica. N, N-isopropylacrylamide is polymerized on the surface of mesoporous silica through a polymerization reaction, and the preparation method comprises the following steps: (1) preparing a curcumin solution, adding modified mesoporous silica powder, fully dispersing, and slowly stirring at 20-25 DEG C under a dark condition until all white nanoparticles become yellow; and (2) collecting the nano-particles in the solution in the step (1), and drying to obtain the modified mesoporous silica drug-loaded particles. According to the curcumin nano-pesticide disclosed by the invention, the carrier N-isopropylacrylamide modified mesoporous silica is introduced, so that the stability, the leaf surface adhesion capability and the bactericidal performance on alternaria longibrachiata of curcumin are improved.

The invention discloses bacillus amyloliquefaciens BA-6 and application of the bacillus amyloliquefaciens BA-6 in prevention and treatment of corn leaf spot disease, and relates to the technical field of screening of biocontrol strains for prevention and treatment of corn leaf spot disease. The strain BA-6 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 27, 2024, the preservation number is CGMCC No.30157, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the strain is classified and named as Bacillus amyloliquefaciens. The strain can effectively antagonize Epicoccum nigrum, Epicoccum broomcorn, Helminthosporium maydis, Alternaria alternata and Helminthosporium bombycis, pot experiments show that fermentation broth inoculated with the strain can improve the prevention and treatment effect on the corn leaf spot disease, and a theoretical basis is provided for subsequent development of a biocontrol microbial inoculum for the corn leaf spot disease.

The invention belongs to the field of microorganisms, and discloses bacillus velezensis and application thereof in prevention and treatment of plant fungal diseases. The bacillus velezensis disclosed by the invention is bacillus velezensis YC46, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.33975. The bacillus velezensis can grow by taking leftovers in an aquatic product processing process as a unique nutrient source, and has the advantages of high yield, low cost and the like. The bacillus subtilis has the antibacterial activity on plant pathogenic fungi such as fusarium oxysporum, botrytis cinerea, alternaria alternata, colletotrichum gloeosporioides and penicillium, thalli or fermentation products of the thalli can be used as active ingredients for preparing biocontrol microbial agents or bacteriostatic agents for preventing and treating plant fungal diseases, and the bacillus subtilis has good application value and ecological environmental protection value.

The invention belongs to the technical field of microorganisms, and discloses bacillus velezensis and application thereof. The strain is separated from saline-alkali soil in the Uygur autonomous region in Xinjiang and is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No.65485. By utilizing a response surface optimization method, the cellulase activity reaches 92.376 U / mL and is improved by 95.6% compared with that of a non-optimized cellulase activity; the growth inhibition rate on alternaria solani, alternaria alternate, fusarium oxysporum and botrytis cinerea hyphae is 88.47-91.08%, and the metabolite (especially an ethyl acetate extract) can significantly inhibit Korla pear diseases caused by alternaria alternate. The strain is resistant to high and low temperature, acid and alkali and salt stress, can degrade straw (the degradation rate is 21.9%), and provides a high-quality strain resource for resource utilization of agricultural wastes and biological control of plant fungal diseases.

The invention discloses a bacillus subtilis SY8 capable of generating myriocin and preventing and treating paeonia lactiflora black spot and application of the bacillus subtilis SY8, the bacillus subtilis SY8 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.33763, and the preservation date is March 10, 2025. The bacillus subtilis SY8 is a biocontrol bacterium, the biocontrol bacterium can generate a natural active substance myriocin (Myriocin) with great value, the bacillus subtilis SY8 has a very strong inhibition effect on the growth of a pathogenic bacterium alternaria alternata of the black spot of the Chinese herbaceous peony, and the bacillus subtilis SY8 is verified to have a very strong prevention and treatment effect on the black spot through living body tieback of the Chinese herbaceous peony, so that the bacillus subtilis SY8 can be used for preventing and treating the black spot of the Chinese herbaceous peony. The strain has good application and development prospects in biological control of black spot of Chinese herbaceous peony and biological synthesis of myriocin.

The invention belongs to the technical field of biopesticides, and relates to a biocontrol fungus and a biocontrol inoculant for preventing and treating anthracnose of camellia oleifera and application of the biocontrol fungus and the biocontrol inoculant. According to the invention, a CV strain of coniochaeta velutina is separated and screened from microbial resources on healthy tea-oil tree leaves, and the CV strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.41439. The invention further discloses a preparation method of the CV strain of the coniochaeta velutina and the application of the CV strain of the coniochaeta velutina. The CV strain provided by the invention has a good antagonistic effect on colletotrichum gloeosporioides, alternaria alternata and botryosphaeria dothidea, and particularly, the bacteriostasis rate of the CV strain on the colletotrichum gloeosporioides, which is a main pathogenic bacterium of camellia oleifera anthracnose, reaches 67.44%. The biocontrol fungus CV strain and the biocontrol inoculant provided by the invention not only can be used for preventing and treating camellia oleifera anthracnose, but also have potential application prospects in the aspect of preventing and treating plant diseases caused by colletotrichum gloeosporioides, alternaria alternata or botryosphaeria dothidea.

The invention discloses a novel leuconostoc screening method and an antibacterial mechanism of leuconostoc on leaf mold of zanthoxylum bungeanum, and relates to the technical field of pathogenic bacteria experiment and analysis. The novel leuconostoc screening method comprises the following steps that S1, materials are prepared, specifically, rotten Chinese prickly ash tree plants are selected, and tested strains in the Chinese prickly ash tree plants comprise plant pathogens of alternaria alternata, fusarium solani, fusarium layered, fusarium oxysporum, fusarium syphilis and colletotrichum pini, and are stored in a refrigerator of a laboratory at the temperature of 4 DEG C for standby application; in the invention, the leuconostoc also has an efficient inhibition effect on field crop pathogenic fungus alternaria alternata, and the research expands the biocontrol spectrum of lactic acid bacteria. Meanwhile, the invention discloses a molecular mechanism that the Leuconostoc suionicum HJ-L synergistically regulates and controls the synthesis of antibacterial substances through lipid metabolism, an ABC transport system and an autophagy pathway, and a theoretical basis and a practical basis are provided for the application of the strain in green prevention and control of the leaf mold of Chinese prickly ash.