154 results about "Alternaria alternata" patented technology

The invention discloses a colorectal cancer plasma circulating microbial marker, a reagent, a kit and application thereof, and belongs to the technical field of biomedical detection. The plasma circulating microbial marker for colorectal cancer comprises alternaria alternata, alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous alophilous The colorectal cancer plasma circulating microbial marker can be used for early screening of colorectal cancer, has relatively high sensitivity and specificity, and remarkably improves the accuracy and reliability of detection; the limitation of traditional ctDNA detection is broken through, and the detection rate of early colorectal cancer is improved. The colorectal cancer plasma circulating microbial marker in a colorectal cancer plasma sample is detected through low-depth whole genome sequencing, the requirement for depth in an experiment is reduced, the cost can be greatly reduced while high sensitivity and specificity are guaranteed, and the method is more suitable for large-scale screening.

The invention discloses trichoderma Guizhou GZMT12 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the trichoderma Guizhou GZMT12 screened by the invention is CGMCC (China General Microbiological Culture Collection Center) NO. 42152. According to the present invention, the bacterial strain can provide inhibition effects on seven pathogenic bacteria such as Rhodococcus solani Kuhn, Botryosphaeria dothidea, Alternania alternania, Phomopsis litsea, Colletotrichum truncatum, Phytotrichum capsici, and Phytotrichum nitianae, and further has advantages of good prevention and treatment effect, wide antibacterial spectrum, wide application range, and the like.

The invention discloses bacillus subtilis S1703 and application thereof, and belongs to the technical field of plant disease and pest control. The name of the bacterial strain is bacillus subtilis S1703, and the preservation number of the bacterial strain is GDMCC No: 66890. The bacterial strain S1703 has a wider antibacterial spectrum, and can effectively inhibit the growth of pathogenic bacteria such as fusarium oxysporum, colletotrichum gloeosporioides, alternaria alternata, aspergillus, phyllosticta, citrus reticulata, fusarium and the like; fermentation liquor of the S1703 has a good antibacterial effect on the psidium guajava wilt pathogen and the psidium guajava branch blight pathogen; the bacteriostasis rate on sigatoka sigatoka and guava leaf spot germs reaches 95% or above, and an excellent bacteriostasis effect is achieved. The strain S1703 can avoid potential adverse effects of chemical pesticides on the environment and crop safety, and has potential commercial development and application values in biological prevention and control practice of diseases.

The application discloses a strain of Mei Ji Maydomyces for delaying early leaf fall of pears and application thereof, and belongs to the technical field of microorganisms. The strain has a preservation number of CGMCC NO.31969 and a preservation date of September 13, 2024. The Mei Ji Maydomyces provided by the application is separated from the surface of healthy pear fruits, is harmless to human bodies, is high in safety, has strong bacteriostatic activity, and can effectively inhibit the growth of early leaf fall pathogenic bacteria of pear, such as Guibao anthracnose, Alternaria alternata and Neofusicoccum parvum. The application makes up for the deficiency of existing plant growth regulators and widens the application range of plant senescence delaying agents.

The invention discloses bacillus amyloliquefaciens BA-6 and application of the bacillus amyloliquefaciens BA-6 in prevention and treatment of corn leaf spot disease, and relates to the technical field of screening of biocontrol strains for prevention and treatment of corn leaf spot disease. The strain BA-6 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 27, 2024, the preservation number is CGMCC No.30157, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the strain is classified and named as Bacillus amyloliquefaciens. The strain can effectively antagonize Epicoccum nigrum, Epicoccum broomcorn, Helminthosporium maydis, Alternaria alternata and Helminthosporium bombycis, pot experiments show that fermentation broth inoculated with the strain can improve the prevention and treatment effect on the corn leaf spot disease, and a theoretical basis is provided for subsequent development of a biocontrol microbial inoculum for the corn leaf spot disease.

The invention belongs to the field of microorganisms, and discloses bacillus velezensis and application thereof in prevention and treatment of plant fungal diseases. The bacillus velezensis disclosed by the invention is bacillus velezensis YC46, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.33975. The bacillus velezensis can grow by taking leftovers in an aquatic product processing process as a unique nutrient source, and has the advantages of high yield, low cost and the like. The bacillus subtilis has the antibacterial activity on plant pathogenic fungi such as fusarium oxysporum, botrytis cinerea, alternaria alternata, colletotrichum gloeosporioides and penicillium, thalli or fermentation products of the thalli can be used as active ingredients for preparing biocontrol microbial agents or bacteriostatic agents for preventing and treating plant fungal diseases, and the bacillus subtilis has good application value and ecological environmental protection value.

The invention belongs to the technical field of microorganisms, and discloses bacillus velezensis and application thereof. The strain is separated from saline-alkali soil in the Uygur autonomous region in Xinjiang and is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No.65485. By utilizing a response surface optimization method, the cellulase activity reaches 92.376 U / mL and is improved by 95.6% compared with that of a non-optimized cellulase activity; the growth inhibition rate on alternaria solani, alternaria alternate, fusarium oxysporum and botrytis cinerea hyphae is 88.47-91.08%, and the metabolite (especially an ethyl acetate extract) can significantly inhibit Korla pear diseases caused by alternaria alternate. The strain is resistant to high and low temperature, acid and alkali and salt stress, can degrade straw (the degradation rate is 21.9%), and provides a high-quality strain resource for resource utilization of agricultural wastes and biological control of plant fungal diseases.

The invention discloses a bacillus subtilis SY8 capable of generating myriocin and preventing and treating paeonia lactiflora black spot and application of the bacillus subtilis SY8, the bacillus subtilis SY8 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.33763, and the preservation date is March 10, 2025. The bacillus subtilis SY8 is a biocontrol bacterium, the biocontrol bacterium can generate a natural active substance myriocin (Myriocin) with great value, the bacillus subtilis SY8 has a very strong inhibition effect on the growth of a pathogenic bacterium alternaria alternata of the black spot of the Chinese herbaceous peony, and the bacillus subtilis SY8 is verified to have a very strong prevention and treatment effect on the black spot through living body tieback of the Chinese herbaceous peony, so that the bacillus subtilis SY8 can be used for preventing and treating the black spot of the Chinese herbaceous peony. The strain has good application and development prospects in biological control of black spot of Chinese herbaceous peony and biological synthesis of myriocin.

The invention discloses a novel leuconostoc screening method and an antibacterial mechanism of leuconostoc on leaf mold of zanthoxylum bungeanum, and relates to the technical field of pathogenic bacteria experiment and analysis. The novel leuconostoc screening method comprises the following steps that S1, materials are prepared, specifically, rotten Chinese prickly ash tree plants are selected, and tested strains in the Chinese prickly ash tree plants comprise plant pathogens of alternaria alternata, fusarium solani, fusarium layered, fusarium oxysporum, fusarium syphilis and colletotrichum pini, and are stored in a refrigerator of a laboratory at the temperature of 4 DEG C for standby application; in the invention, the leuconostoc also has an efficient inhibition effect on field crop pathogenic fungus alternaria alternata, and the research expands the biocontrol spectrum of lactic acid bacteria. Meanwhile, the invention discloses a molecular mechanism that the Leuconostoc suionicum HJ-L synergistically regulates and controls the synthesis of antibacterial substances through lipid metabolism, an ABC transport system and an autophagy pathway, and a theoretical basis and a practical basis are provided for the application of the strain in green prevention and control of the leaf mold of Chinese prickly ash.

A microbial composite strain H2, antagonistic to multiple potato pathogens, and its application and preparation method, relates to the field of biological control. The microbial composite strain H2 is a mixture of Bacillus subtilis, Bacillus atrophaeus, and Bacillus Widmannii. It exhibits strong inhibitory effects against potato early blight pathogens Alternaria solani and Alternaria alternata; wilt / dry rot pathogens Fusarium solani, Fusarium oxysporum, and Fusarium equiseti; and leaf / stem spot pathogen Vitis vinifera, with inhibition rates exceeding 46%. The highest inhibition rate against Alternaria alternata reached 75.97%. H2 also exhibits significant growth-promoting and yield-increasing effects, providing a high-quality strain for the comprehensive control of soil-borne potato diseases and promising prospects for field application.

The invention discloses a sodium alginate-quaternary ammonium salt cationic surfactant pesticide adjuvant as well as a preparation method and application thereof, and belongs to the technical field of pesticide adjuvants. The invention discloses a sodium alginate-quaternary ammonium salt cationic surface active agent pesticide adjuvant. The sodium alginate-quaternary ammonium salt cationic surface active agent pesticide adjuvant is prepared from sodium alginate and a quaternary ammonium salt cationic surface active agent. The quaternary ammonium salt cationic surface active agent and the sodium alginate can form the pesticide adjuvant with uniform size through simple electrostatic attraction and hydrophobic interaction between surface active agent carbon chains. Tebuconazole added with the pesticide adjuvant has a certain synergistic effect on the antibacterial activity of alternaria alternata, fusarium oxysporum and botrytis cinerea. And the auxiliary agent is non-toxic to the germination of plants, and accords with the development trend of green pesticides.

The present application belongs to the technical field of plant disease prevention and treatment, and particularly relates to a biocontrol bacterium for preventing and treating Guaiacum officinale anthracnose, a fermentation liquor, a biocontrol bacterium agent and application. Bacillus velezensis A biocontrol bacterium is isolated and screened from a microbial resource on a healthy Guaiacum officinale leaf, and is classified and named as Bacillus velezensis , which is preserved in the China General Microbiological Culture Collection Center on March 28, 2025, and has a preservation number of CGMCC No.34038; the biocontrol bacterium provided in the present application has good antagonistic effects on Colletotrichum gloeosporioides causing Guaiacum officinale anthracnose, on Stemphylium botryosum causing Guaiacum officinale leaf spot disease, on Botryosphaeria dothidea, on Alternaria alternata and on Pestalotiopsis versicolor capable of causing plant diseases. The biocontrol bacterium, the fermentation liquor and the biocontrol bacterium agent provided in the present application have potential application prospects in preventing and treating Guaiacum officinale anthracnose.

The invention discloses a novel functional microbiome fortified yeast and application thereof in improving the quality of Shanxi mature vinegar. The invention provides a combined bacterium, which is prepared from Aspergillus sp. (ACCC 34193), Saccharomycopsis fibulligera (9203) ACCC 21511, Lichtheimia ramosa (5977) ACCC 34192, Bacillus velezensis (9386) ACCC 64506, and Lactobacillus plantarum (9600) ACCC 64531, and the combined bacterium is prepared from the following raw materials: Aspergillus sp. (9177), Saccharomycopsis fibulligera (9203), ACCC 21511, Lichtheimia ramosa (5977), ACCC 64506, and Lactobacillus plantarum (9600). The invention also provides a fortified yeast containing the combined bacteria. Compared with the traditional vinegar koji, the Shanxi mature vinegar prepared by using the strengthened koji disclosed by the invention not only improves the saccharification power, flavor and appearance of the vinegar koji, but also effectively improves the quality, flavor and functional substance content of the mature vinegar.

The invention relates to the field of ginseng black spot research, in particular to a slicer for ginseng black spot research, which comprises a placing mechanism, a lifting mechanism is arranged in the placing mechanism, and one end of the lifting mechanism penetrates and extends into the placing mechanism and is fixedly provided with a cutting mechanism. The top end of the lifting mechanism penetrates and extends out of the containing mechanism and is fixedly connected with a positioning mechanism, and a supporting mechanism is arranged at the top end of the interior of the containing mechanism. The ginseng can be placed in the placing groove, the pressing plate is pressed down to fix the ginseng, then the ginseng is cut through the cutting knife, the cut ginseng slices are taken out through the grid baffle, black spot on the ginseng can be observed, and the thickness of the next ginseng slice needing to be cut can be determined. Therefore, the height of the cutting knife is adjusted again to cut the ginseng slices with the corresponding thickness, and follow-up research is facilitated.

The invention relates to a preparation for preventing and / or treating sugarcane leukosis and application thereof, and relates to the technical field of biological agents. The preparation comprises bacillus mucilaginosus, bacillus megatherium, bacillus laterosporus, alternaria tenuissima activator protein, calcium acetate, magnesium sulfate and glucose. The preparation has a relatively good prevention and / or treatment effect on the sugarcane bacterial leaf streak, and also can promote the growth of the sugarcane at the same time.

This invention belongs to the field of biodetection technology, specifically relating to a primer set, kit, and application for detecting *Alternaria solani* brown spot disease. The base sequences of the primer set are shown in SEQ ID NO.1~SEQ ID NO.5. Based on the primer set, this invention utilizes loop-mediated isothermal amplification technology for the rapid detection of *Alternaria solani*, accurately detecting the fungus from the complex pathogenic environment in diseased plant tissues. The rapid detection system described in this invention exhibits high specificity and sensitivity, with a detection limit of 0.246 fg / μL for *Alternaria solani* DNA. Using the rapid detection system provided by this invention, *Alternaria solani* can be detected rapidly, conveniently, accurately, and sensitively from field samples within 45 minutes, which is simpler and more efficient than traditional symptom identification methods and ordinary molecular detection methods.

The present application relates to the technical field of biology, and in particular discloses a PCR detection primer pair of Alternaria porri, a target and application thereof.The present application provides a PCR detection primer pair of Alternaria porri, and the nucleotide sequences of the primer pair are shown as SEQ ID No.1-2.After PCR amplification of DNA of a to-be-tested sample is carried out by using the primer pair of the present application, and then whether specific bands appear or not is observed by combining with gel electrophoresis, it can be determined whether Alternaria porri is contained in the to-be-tested sample or not.The primer has high specificity and good sensitivity, can rapidly detect the presence of Alternaria porri in a sample, and can provide a reference for early inspection and quarantine and early prevention and treatment of Alternaria porri leaf spot.

The present application relates to a kind of new compound and its preparation method and application, specifically 1-substituted phenyl-5-methyl-1 H Preparation and application of pyrazole-3-formamide derivatives. The structural formula of the compound 1-substituted phenyl-5-methyl-1 H Pyrazole-3-formamide derivatives is as shown in formula I. Wherein, R1 selected from: 4-chloro or 2,4-dichloro;R2 selected from: H, methyl, methoxy, cyclopropyl;R3 selected from: 3-methylphenyl, 3-nitrobenzyl, 4-nitrobenzyl, 4-chlorophenyl, 2-(5-chloro-1 H Indole-3-yl) ethyl, N-(4-chlorophenyl)-2-phenyl. Test shows that the compound of the application shows good antifungal activity to grape cavity fungus (Botryosphaeria dothidea) (Botryosphaeria dothidea), Pythium aphanidermatum (Pythium aphanidermatum), Fusarium oxysporum (Fusarium oxysporum), Gray pear spot fungus (Gymnosporangium japonicum), Alternaria alternata (Alternaria alternata), Sclerotinia sclerotiorum (Sclerotinia sclerotiorum), Botrytis cinerea (Botrytis cinerea), and has wide application prospect. Botryosphaeria dothidea Pythium aphanidermatum Fusarium oxysporum Phyricularia grisea Alternaria nees Sclerotinia sclerotiorum Botrytis cinerea ​​​​​​​

The invention relates to a salt-tolerant bacillus strain EMM919, which is preserved in the China Center for Type Culture Collection on April 25, 2025, and the preservation number is CCTCC NO: M 2025879. The salt-tolerant bacillus strain EMM919 has the advantages that the salt-tolerant bacillus strain EMM919 is preserved in the China Center for Type Culture Collection on April 25, 2025; the nucleotide sequence of the strain comprises a 16S rRNA (ribosomal Ribonucleic Acid) gene sequence and a gyrB gene sequence. The bacterial strain can be used for preparing microbial pesticides and seed dressing agents for preventing and treating fusarium solani EMF893, neurospora EMF992, cylindrospora robusta EMF882, fusarium laminatum EMF998, alternaria alternata EMF993, botryosphaeria dothidea EMF899, solanum nigrum EMF990, phomopsis EMF898 and fusarium graminearum EMF897. The content of viable bacteria in the liquid pesticide is 2 * 10 < 9 >-5 * 10 < 9 > CFU / mL, and the content of viable bacteria in the solid pesticide is 2 * 10 < 9 >-8 * 10 < 9 > CFU / g. In the liquid seed-dressing agent, the content of viable bacteria is 2 * 10 < 9 >-5 * 10 < 9 > CFU / mL, and in the solid seed-dressing agent, the content of viable bacteria is 2 * 10 < 9 >-8 * 10 < 9 > CFU / g.

The present application relates to the technical field of pharmaceutical chemistry, and particularly relates to application of tryptophanone in prevention and treatment of plant bacteria and part of plant fungi. The present application discloses that natural product tryptophanone can effectively prevent and treat plant bacterial and part of plant fungal diseases. The application of tryptophanone as an agricultural bactericide in plant bacterial diseases has excellent inhibitory effect on rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, cabbage black rot, tomato bacterial spot, kiwi canker, tobacco bacterial wilt, cucumber bacterial angular spot, potato bacterial wilt, soybean bacterial spot, tomato canker and the like. The tryptophanone also shows good inhibitory activity on plant pathogenic fungi such as grapevine Eutypa lata, tobacco alternaria alternata, eggplant verticillium wilt, pepper fusarium wilt, colletotrichum gloeosporioides, tea tree colletotrichum gloeosporioides and sorghum zylosporium.

The present application belongs to the technical field of fertilizer, and specifically discloses a foliar fertilizer for improving the disease resistance effect of Vietnamese ginseng, which is composed of 0.1-0.5% potassium dihydrogen phosphate, 0.1-0.2% boric acid, 0.3-0.7% urea, 0.005-0.02% chitosan, 0.0001% salicylic acid and 0.05% Tween-20, and the rest is water, according to the weight percentage. The foliar fertilizer prepared by the present application has a remarkable disease control effect on Vietnamese ginseng, and has a positive control effect on the infection of Fusarium oxysporum, Alternaria panax and Botryosphaeria dothidea. After using the foliar fertilizer of the present application, the growth of the pathogen is significantly inhibited from the phenotype, and the disease onset time of Vietnamese ginseng is significantly later than that of the control. The resistance gene expression of Vietnamese ginseng is significantly improved through testing.

The invention discloses Acetobacter peach and application thereof in prevention and treatment of postharvest diseases, and belongs to the technical field of microorganisms and the field of biological prevention and treatment of postharvest diseases. The Acetobacter persici NM-1 disclosed by the invention has an antagonistic effect on telosporium roseum and alternaria alternata, and has a remarkable prevention and treatment effect on postharvest diseases of apples such as red rot and black spot. The invention provides a biocontrol bacterium resource for prevention and treatment of apple red rot and black spot.

The invention discloses a bacillus PY4 strain capable of efficiently antagonizing various phytopathogens and application of the bacillus PY4 strain, and belongs to the technical field of biological prevention and control of plant diseases. The strain has stable morphological characteristics, and is identified according to a 16S rRNA (ribosomal Ribonucleic Acid) sequence, and the strain is a Paenibacillus bacterium. An in-vitro confrontation test proves that the PY4 can effectively inhibit pathogenic fungi such as sclerotinia sclerotiorum, fusarium graminearum, pseudocauda sp, alternaria alternata and sclerotium rolfsii. Field application results show that the PY4 has a good prevention and treatment effect on sclerotinia rot of colza. The invention further provides a biocontrol agent based on the strain and an application method of the biocontrol agent. The biocontrol agent can be used for green prevention and control of soil-borne and leaf-borne fungal diseases of crops.

The application discloses a tobacco endophytic fungus metabolite, a preparation method and application in tobacco alternaria alternata prevention and treatment, and relates to the technical field of microbial natural product pesticides. The tobacco endophytic fungus metabolite is a benzoquinone compound peniquinone L, and a structural formula is as follows: The benzoquinone compound peniquinone L is produced by fermentation of tobacco endophytic fungus Aspergillus aculeatus Aspergillus aculeatus Aa-1, and scale fermentation and preparation production are easy to carry out. The tobacco endophytic fungus metabolite benzoquinone compound peniquinone L of the application has inhibitory activity on various agricultural pathogens, and has significant bacteriostatic activity on tobacco alternaria alternata in particular, with an MIC value of 2 μg / mL, which is superior to positive drug carbendazim, and can be used as a leading compound or a new microbial natural product pesticide for tobacco alternaria alternata prevention and treatment.

The invention discloses an endophytic fungus with functions of improving stress resistance and promoting growth of fraxinus mandshurica and application of the endophytic fungus, and belongs to the technical field of microorganisms, the endophytic fungus is named as Alternaria tenuissima SLR21 and preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO. 42057. The endophytic fungus has the advantages that the endophytic fungus is named as Alternaria tenuissima SLR21; according to the invention, fraxinus mandshurica is taken as a material, the endophytic fungus with the functions of improving stress resistance and promoting growth of fraxinus mandshurica is screened out, a new microbial resource is provided for digging a new strain with a biological energy function, and a new path is provided for solving the problem that fraxinus mandshurica forest establishment is difficult. Fermentation liquor of the endophytic fungi contains substances with high content of polyphenol, polysaccharide, flavone, salicylic acid, auxin and the like, has high drought resistance, salt and alkali resistance and the function of promoting seedling plant height increase, can be independently used as a seed soaking agent to be applied to promoting seed germination, and can also be used for carrying out root-irrigation or spraying treatment on nursery stocks through fermentation liquor of the endophytic fungi.

The invention provides a strain of Chryseobacterium ZJU645, a biological preparation, a culture method, a fermentation method and application, and particularly belongs to the technical field of microbial control. The Chryseobacterium sp. ZJU645 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO.35186, and the preservation date is July 10, 2025. The Chryseobacterium ZJU645 disclosed by the invention has a broad-spectrum antifungal effect, and has a relatively high antibacterial effect on alternaria alternata, sclerotinia sclerotiorum, fusarium oxysporum, paniculate corn, colletotrichum gloeosporioides and botrytis cinerea, and a fermentation product of the Chryseobacterium ZJU645 is stable. Through further optimization of fermentation conditions of the Chryseobacterium sp. ZJU645, the disease resistance of the Chryseobacterium sp. ZJU645 is improved, and a new biological control means is provided for more efficient prevention and treatment of agricultural diseases, especially mulberry diseases.

The present application discloses a Streptomyces pseudovenezuelae and application of the Streptomyces pseudovenezuelae as a biocontrol agent in preventing and treating plant diseases. Streptomyces pseudogriseolus The Streptomyces pseudovenezuelae M5 screened by the present application is preserved in China Center for Type Culture Collection, and the preservation registration number is CCTCC NO: M 20252042. The Streptomyces pseudovenezuelae M5 screened by the present application has a strong inhibiting effect on a plurality of filamentous fungal pathogenic bacteria, has an obvious prevention and treatment effect on leek leaf rot disease caused by Alternaria alternata, and has an obvious effect on a newly discovered pathogenic bacterium Microdochium dimidiatum on blueberries. The M5 has a good biocontrol function and a growth promotion effect, has an excellent application prospect as a biocontrol agent for preventing and treating plant diseases, and especially provides an effective biocontrol preparation for diseases caused by the newly discovered pathogenic bacterium Microdochium dimidiatum on blueberries.

This invention belongs to the field of biotechnology, specifically disclosing a rapid sporulation method for *Alternaria ginseng*. The method involves activating and culturing *Alternaria ginseng* in PDA medium at 25°C in the dark for 10 days. Mycelial cakes are then collected from the colony edges using a punch. These mycelial cakes are inoculated onto a sporulation medium and cultured in a sealed dark environment at 22°C for 4 days. After making crisscross cuts 1 cm from the center of the colony, the cells are treated with ultraviolet light for 9 minutes. The culture dish is then left uncovered and placed at 22°C. Finally, the cells are cultured in a light incubator at 70% relative humidity for 12 hours followed by 12 hours in the dark, resulting in rapid sporulation at the cuts. Compared to existing technologies, this invention significantly shortens the sporulation cycle (5 days), greatly saving time and costs.

The present invention belongs to the field of biological genetic engineering and relates to the use of ginseng transcription factor PgERF2 in improving plant resistance to Alternaria alternata. The sequence of the ginseng transcription factor PgERF2 protein is shown in SEQ ID NO.1; the sequence of the ginseng transcription factor PgERF2 gene is shown in SEQ ID NO.2. The present invention clones the PgERF2 gene and constructs a corresponding plant expression vector, successfully transferring the gene into wild-type Arabidopsis thaliana. Transcriptomic analysis results show that the response to biotic stress is activated in the transgenic plants, and the expression level of the plant defense protein-related gene AtPDF1.2A is significantly increased. It is also demonstrated that PgERF2 can directly bind to the GCC-box element to activate the expression of the plant defense protein-related gene AtPDF1.2A, thereby significantly enhancing resistance to Alternaria alternata.

The application discloses a kind of steroidal compounds with anti-parkinson disease activity and its preparation method and application.The steroidal compound has the structure as shown in formula (I) or formula (II), or stereoisomer, tautomer, pharmaceutically acceptable salt or prodrug of the structure shown in formula (I) or formula (II).The application is fermented by fungal co-cultivation to alternaria brassicae and penicillium granatum, and the expression of silent gene of strain is promoted by adopting co-cultivation and optimizing medium condition strategy, novel secondary metabolite is obtained, and new steroidal compound with treatment parkinson disease value and application prospect is extracted and separated from co-cultivation ferment, which provides great prospect for the research and development of parkinson disease treatment drugs.