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Preparation method of surface-protein-C1 polyclonal antibody of cryptocaryon irritans brown and application of surface-protein-C1 polyclonal antibody

A technology for stimulating Cryptonucleus and polyclonal antibodies, which is applied in the field of bioengineering and can solve the problems of limiting the interaction between the worm and the host, and lack of stimulating the labeled antibody of Cryptonucleus.

Inactive Publication Date: 2017-09-05
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
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Problems solved by technology

However, there is still a lack of labeled antibodies that stimulate Cryptocaryonia, which limits the study of parasite-host interactions

Method used

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  • Preparation method of surface-protein-C1 polyclonal antibody of cryptocaryon irritans brown and application of surface-protein-C1 polyclonal antibody
  • Preparation method of surface-protein-C1 polyclonal antibody of cryptocaryon irritans brown and application of surface-protein-C1 polyclonal antibody
  • Preparation method of surface-protein-C1 polyclonal antibody of cryptocaryon irritans brown and application of surface-protein-C1 polyclonal antibody

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Embodiment Construction

[0092] The present invention will be further described below in conjunction with specific embodiments, and the advantages and characteristics of the present invention will become clearer along with the description. However, the examples are merely exemplary and do not limit the scope of the present invention in any way. Those skilled in the art should understand that the details and forms of the technical solutions of the present invention can be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the protection scope of the present invention.

[0093] A method for preparing a polyclonal antibody stimulating Cryptocaryon surface protein C1, the specific steps are as follows:

[0094] 1. Stimulate Cryptocaryon subculture:

[0095] The Cryptocaryon stimuli used were isolated from an oval pomfret breeding cage in Daya Bay, Huizhou City, Guangdong Province. Microscopic examination was ...

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Abstract

The invention discloses a preparation method of surface-protein-C1 polyclonal antibody of cryptocaryon irritans brown and an application of the surface-protein-C1 polyclonal antibody. It is discovered by us study that a surface protein C1 gene of cryptocaryon irritans brown is a potentially effective protective antigen gene; an expression vector of rDNA of tetrinophore is established, the surface protein C1 gene of the cryptocaryon irritans brown is subjected to vitro expression and separation and purification of recombinant protein, the surface-protein-C1 polyclonal antibody is finally prepared, and the primary application of the surface-protein-C1 polyclonal antibody in terms of immunohistochemistry is performed.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for preparing a polyclonal antibody that stimulates the surface protein C1 of Cryptocaryon. Background technique [0002] Cryptocaryon irritans is a ciliate that parasitizes marine fish and causes a serious fatal disease of marine fish—white spot disease. In recent years, due to the overloaded development of marine aquaculture in my country and the deterioration of the aquaculture environment, Cryptocaryoniasis has become a large-scale, frequent outbreak of severe infectious disease, causing very serious harm to seawater fishery. Due to the open nature of mariculture, it is difficult to eliminate the infection of Cryptocaryon irritant to fish, and there is no effective treatment drug and treatment method, which makes the prevention and treatment of this disease extremely difficult. [0003] Drug control and physical control (Colorni and Burgess, 1997;...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/20C07K16/06C07K14/44C12N15/30G01N33/68
CPCC07K16/065C07K16/20
Inventor 李言伟但学明莫泽权
Owner SOUTH CHINA AGRI UNIV
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