Production method for cryptocaryon irritant prevention epinephelus coioides seedling
A technology for slanting grouper and stimulating Cryptonucleus, which is applied in the field of anti-stimulating Cryptonucleus fry production and disease-resistant fry production. It can only reach 3 months, etc., to achieve the effect of long effective immune protection period, simple and easy operation, and improved breeding efficiency.
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[0026] 1. Stimulation of live Cryptocaryon larvae collection and concentration calculation
[0027] Use a special Cryptocaryon stimuli subculture system to subculture and collect Cryptocaryon stimuli. The subculture host is Pomfret ovata. Collect well-developed Cryptocaryon stimuli cysts and place them in a sterilized clean beaker for incubation. The hatching water is high-pressure sterilized natural seawater with an appropriate amount of penicillin and streptomycin added; collect the stimulated Cryptocaryon larvae liquid within 2 hours of hatching, mix thoroughly, take 50 μL of the larvae, and add 10 μL of formaldehyde to kill the larvae. Count under a microscope, and record the live worm concentration in the larva liquid.
[0028] 2. Domestication and infection of grouper fry
[0029]Purchase about 11g of grouper fry, and test the fry for domestication 2 weeks before infection until they eat and move normally. During the whole breeding process, the breeding water is filtere...
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