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Production method for cryptocaryon irritant prevention epinephelus coioides seedling

A technology for slanting grouper and stimulating Cryptonucleus, which is applied in the field of anti-stimulating Cryptonucleus fry production and disease-resistant fry production. It can only reach 3 months, etc., to achieve the effect of long effective immune protection period, simple and easy operation, and improved breeding efficiency.

Inactive Publication Date: 2016-10-12
SOUTH CHINA AGRI UNIV +1
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AI Technical Summary

Problems solved by technology

In recent years, researchers have carried out some basic research and applied research on the immune control of Cryptocaryon stimuli, and found that the larvae of Cryptocaryon stimuli can be used to infect seawater fish such as oblique grouper and pomfret through the body surface, which can reach 80%. The above immune protection rate (Luo et al, 2007; Dan Xueming et al, 2008; Yoshinaga et al, 1997), but the effective immune protection period of these experiments can only reach 3 months at most, and there is no detailed study on the effect of different temperatures on seawater Effects of Fish Acquired Immunity Protection

Method used

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  • Production method for cryptocaryon irritant prevention epinephelus coioides seedling
  • Production method for cryptocaryon irritant prevention epinephelus coioides seedling

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Embodiment Construction

[0026] 1. Stimulation of live Cryptocaryon larvae collection and concentration calculation

[0027] Use a special Cryptocaryon stimuli subculture system to subculture and collect Cryptocaryon stimuli. The subculture host is Pomfret ovata. Collect well-developed Cryptocaryon stimuli cysts and place them in a sterilized clean beaker for incubation. The hatching water is high-pressure sterilized natural seawater with an appropriate amount of penicillin and streptomycin added; collect the stimulated Cryptocaryon larvae liquid within 2 hours of hatching, mix thoroughly, take 50 μL of the larvae, and add 10 μL of formaldehyde to kill the larvae. Count under a microscope, and record the live worm concentration in the larva liquid.

[0028] 2. Domestication and infection of grouper fry

[0029]Purchase about 11g of grouper fry, and test the fry for domestication 2 weeks before infection until they eat and move normally. During the whole breeding process, the breeding water is filtere...

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Abstract

The invention relates to a production method for cryptocaryon irritant prevention epinephelus coioides seedlings, and belongs to the field of disease resistance fry production, and specifically relates to the field of cryptocaryon irritant prevention fry production. A method of varying temperature infection and varying temperature culture is firstly used to produce disease resistance epinephelus coioides seedlings. Water temperature in infection and cultivation water temperature in 10 days after infection keep certain temperature difference, so as to enhance immune effect of cryptocaryon irritant live larva, to further combine with rotational culture. The method obtains cryptocaryon irritant prevention epinephelus coioides seedlings in a relatively long effective immune protection period. In the eighth month after immunity, the seedlings still have relative protection rate of 60%, and in the ninth month, the seedlings still have relative protection rate of 34%.

Description

technical field [0001] The technology relates to a production method of anti-stimulant Cryptocaryon sclerensis fry, belongs to the field of disease-resistant fry production, specifically the field of anti-stimulant Cryptocaryon fry production. Background technique [0002] The oblique-banded grouper (Epinephelus coioides) is a fish belonging to the genus Epinephelus, commonly known as green spot. The oblique-banded grouper is a warm-water fish, mainly distributed in tropical and subtropical waters. Due to its excellent economic characteristics such as low oxygen consumption rate, high feed conversion rate, fast growth rate, and high nutritional value, it has become an important seawater cultured fish in the southern coast of China. [0003] Cryptocaryon irritans, commonly known as marine fish white spot disease, is a deadly parasitic disease caused by an obligate parasitic ciliate of marine fish——Cryptocaryon irritans (Colorni and Burgess, 1997) . In recent years, Cryptoc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCA01K61/00Y02A40/81
Inventor 但学明李安兴李言伟莫泽权
Owner SOUTH CHINA AGRI UNIV
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