The invention discloses a chlorhexidine gluconate compound gargle and a preparation method thereof. The chlorhexidine gluconate compound gargle is made of the following raw materials in parts by weight: polysorbate 80 is 5.5-6 parts, formazan 0.8-1.2 parts of nidazole powder, 220-225 parts of ethanol, 310-315 parts of glycerin, 275-300 parts of polyethylene glycol 400, 115-125 parts of chlorhexidine gluconate, 42.225-72.728 parts of excipients, Appropriate amount of purified water. The raw materials of the invention complement each other and play a synergistic role, which can assist in the prevention and treatment of oral diseases and improve the effect of oral care. In the preparation process of the chlorhexidine gluconate compound gargle of the present invention, the stability of the product is improved by adjusting the amount of auxiliary materials, the feeding method and even the order of feeding, which not only greatly reduces the generation of the harmful substance p-chloroaniline in the preparation process , and effectively solve the existing chlorhexidine gluconate compound gargle in the preparation process, which is easy to produce turbidity, crystallization and other quality problems.