Application of honokiol in preparation of medicines for inhibiting and killing in vitro parasitic ciliates of fish

A technology of honokiol and ciliates, applied in the field of biological control, can solve the problems of pathogen resistance, drug residues, environmental pollution, etc., achieve the effects of small toxic and side effects, easy degradation and elimination, and broad application prospects

Active Publication Date: 2018-03-20
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, seawater melon worm disease is mainly controlled by chemical drugs such as mercurous nitrate, formaldehyde or methylene blue. However, these chemical drugs have negative effects such as pathogen resistance, environmental pollution, and drug residues. It is urgent to find environmentally friendly and safe drugs. efficient control method
At present, there is no relevant report on the antiparasitic pharmacological effects of honokiol

Method used

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  • Application of honokiol in preparation of medicines for inhibiting and killing in vitro parasitic ciliates of fish
  • Application of honokiol in preparation of medicines for inhibiting and killing in vitro parasitic ciliates of fish
  • Application of honokiol in preparation of medicines for inhibiting and killing in vitro parasitic ciliates of fish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1, honokiol is to the inhibitory action of stimulating Cryptocaryonia infective larvae

[0018] (1) Drug preparation: 50% ethanol was used as a solvent to prepare honokiol solutions with concentrations of 0.00, 0.01, 0.02, 0.04, 0.06, 0.08 and 0.10 mg / mL.

[0019] (2) Take a 24-well plate, add 940 μL of sterile seawater filtered through a 0.22 μm filter membrane to each well, absorb 50 μL of stimulated Cryptocaryon larvae within 30 minutes of cyst incubation, and add them to the 24-well cell culture plate, and the number of worms is 100-200 Then add 10 μL of honokiol solutions with different concentrations, so that the final concentrations of honokiol in the system are 0.0, 0.1, 0.2, 0.4, 0.6, 0.8 and 1.0 μg / mL, respectively. Three wells were run in parallel for each drug concentration. Observe the death of Cryptocaryon larvae within 30 minutes of stimulation under an inverted optical microscope with a 50-fold mirror, and calculate the lethality rate. Letha...

Embodiment 2

[0022] Embodiment 2, honokiol is to the inhibitory killing effect of stimulation Cryptocaryon trophozoite

[0023] (1) Preparation of medicine: Same as in Example 1, configured as 0.00, 0.05, 0.10, 0.50, 1.00, 1.50 and 2.00 mg / mL honokiol liquid medicine.

[0024] (2) Take a 24-well cell culture plate, add 2 mL of sterile seawater filtered through a 0.22 μm filter membrane to each well, absorb and stimulate Cryptocaryon trophozoites into the cell culture plate, 20 per well, and then add 20 μL of different concentrations Honokiol medicinal solution, so that the final concentrations were 0.0, 0.5, 1.0, 5.0, 10.0, 15.0 and 20.0 μg / L, respectively, with 3 parallel wells for each concentration, and the 24-well cell culture plate was placed in a 27°C light incubator. Culture in 12h light and 12h dark, change 1mL of water at 8:00 every day, 2 days later the trophozoites form cysts, and 7 days after the formation of cysts, observe the hatching of the cysts under a 40x stereo microscop...

Embodiment 3

[0027] Embodiment 3, honokiol is to the inhibitory killing effect of stimulating Cryptocaryon cyst

[0028] (1) Preparation of medicine: Same as Example 1, prepared into 0.000, 0.005, 0.010, 0.025, 0.050 and 0.100mg / mL honokiol liquid medicine.

[0029] (2) Take a 24-well cell culture plate, add 2 mL of sterile seawater filtered through a 0.22 μm filter membrane to each well, absorb and stimulate Cryptocaryon cysts into the cell culture plate, 20 per well, and then add 20 μL of different concentrations and The magnolol solution was used to make the final concentrations of 0.00, 0.05, 0.10, 0.25, 0.50 and 1.00 μg / mL, respectively, and 3 wells were paralleled for each concentration. The cell culture plate was placed in a light incubator at 27°C for 12 hours of light and 12 hours of darkness, and 1 mL of water was changed at 8:00 every day. The cyst hatching was observed under a 40-fold stereo microscope, and the cyst hatching rate was counted within 7 days. Hatching rate (%) = ...

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Abstract

The invention belongs to the field of biological prevention and control, and relates to application of honokiol in preparation of medicines for inhibiting and killing in vitro parasitic ciliates of fish. The invention discloses activity of the honokiol in inhibition of the in vitro parasitic ciliates of the fish, and applies the honokiol to prepare the medicinesthe application of the honokiol in preparation of the medicines for inhibiting and killing the in vitro parasitic ciliates of the fish; the honokiol is especiallyused for inhibiting and killing cryptocaryon irritans brown, the honokiolcan completely inhibit and kill parasites within an effective dose range, and achieves the effect of preventing and controlling seawater ichthyophthiriasis; the honokiol is a natural compound, is little in toxic and side effects and easy to degrade and remove, can avoid the problems such as environmental pollution and drug residue which are caused by abuse of chemicals, and is beneficial to promotepromotion of the sound development of aquaculture industry.

Description

technical field [0001] The invention belongs to the field of biological control and relates to honokiol, in particular to the application of honokiol as a main ingredient in the preparation of fish medicines for inhibiting and killing ectoparasitic ciliates. Background technique [0002] Fish diseases caused by parasitic ciliates are common and frequently-occurring diseases in aquaculture. Cryptocaryon irritans Brown is a ciliate that can parasitize bony fishes in tropical and subtropical seawater. Seawater melon worm disease, also known as "white spot disease", is a parasitic disease caused by Cryptocaryon irritans Brown. When seawater fish are infected and stimulated by Cryptocaryon, visible "white spots" appear on the body surface and gills, leading to severe hypoxia, damage to the body surface and secondary bacterial infection, which in turn leads to the death of a large number of fish. In recent years, the degree of intensive seawater fish farming in my country has gr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/05A61P33/14
CPCA61K31/05
Inventor 郭伟良周永灿钟志鸿王菲王世锋孙云
Owner HAINAN UNIVERSITY
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