15 results about "Ichthyophthiriasis" patented technology

The invention discloses application of ursolic acid in resisting fish ichthyophthiriasis. The ursolic acid has a remarkable killing effect on ichthyophthirius multifilis in an in-vitro stage, and the 4-hour half effective insecticidal concentration (4h-EC50) of the ursolic acid on a grazing body is 0.3774 mg / L; and the 22-hour half effective insecticidal concentration (22h-EC 50) on the capsule is 4.931 mg / L. An acute toxicity test shows that the 96-hour median lethal concentration (96h-LC50) of ursolic acid to goldfish is 9.358 mg / L, the therapeutic index (TI = 96h-LC50 / 4h-EC50) is up to 24.8, and the safety of ursolic acid is remarkably superior to that of a traditional medicine. Ursolic acid used in the invention is a plant-derived active component, has the characteristics of high environmental compatibility and low biological accumulation, meets the development requirements of green fishery, and has an industrialization basis for being developed into eco-friendly aquatic products.

The invention relates to application of procyanidine in preparation of a medicine for inhibiting and killing in-vitro parasitic ciliates of fishes, and belongs to the technical field of medicines for fish parasitic diseases. The procyanidine is applied to preparation of drugs for inhibiting and killing in-vitro parasitic ciliates of fishes. The procyanidine is applied to preparation of the medicine for inhibiting and killing the in-vitro parasitic ciliates of the marine fishes for the first time, especially for inhibiting and killing cryptocaryon irritans, the in-vitro parasites can be completely inhibited and killed within an effective dosage range, and the effect of preventing and treating the ichthyophthiriasis of the marine fishes is achieved. The procyanidine serving as a main medicine component is wide in source, has an industrial preparation process, is low in price, has a huge potential of being developed into a novel fish medicine for preventing and treating in-vitro parasitic ciliates of fishes, and is wide in application prospect. The procyanidine has small toxic and side effects, is easy to degrade and eliminate, can effectively avoid negative effects such as environmental pollution and drug residues, and promotes sustainable, green and healthy development of the aquaculture industry.

The invention discloses an application of alkannin in resisting fish ichthyophthiriasis. The alkannin has remarkable killing activity on ichthyophthirius multifilis in an in-vitro stage, the 4-hour half effective insecticidal concentration (4 h-EC50) of the alkannin on a grazing body is 0.048 mg / L, and the 22 h-EC50 of the alkannin on a cyst is 1.275 mg / L. Besides, the shikonin has a 96-hour median lethal concentration (96h-LC50) of 2.229 mg / L to goldfish and a treatment index (TI) of 46.4 to a grazing body, and can be safely applied to fishes in an effective insecticidal concentration range. The shikonin disclosed by the invention has the characteristics of high insecticidal activity and low toxicity to fishes, has the potential of being developed into novel green fish ichthyophthiriasis-resistant aquatic products such as fish medicines, feed additives and the like, and has a wide application prospect.

The invention discloses a method for preventing and treating ichthyophthiriasis of industrially cultured snakehead fries, which belongs to the technical field of snakehead culture and comprises the following steps: completely catching snakehead fries infected with ichthyophthiriasis in a culture pond, and transferring the snakehead fries into a biological bait culture pond for isolated culture; transferring the snakeheads subjected to isolated culture to a culture pond, and raising the salinity of the culture pond for culture; in the same period of culturing in the culture pond, the composite Chinese herbal medicine preparation is splashed into the culture pond to conduct soaking treatment on the snakeheads; after treatment is finished, in daily culture in a culture pond, a red-knees herb decoction water solution is splashed into the culture pond to consolidate the treatment effect of the snakehead and prevent the snakehead. Through the synergistic effect of sick fish isolation, salinity regulation and control and red-knees herb treatment, the survival rate and quality of fish culture are remarkably improved, the culture yield is increased, economic benefits are improved, ecological benefits are improved, and powerful support is provided for green development of factory fish culture.

The invention discloses application of rhizoma osmundae in prevention and treatment of ichthyophthiriasis of aquaculture animals, toxicity experiments of rhizoma osmundae on grass carp kidney cells (CIK) show that the maximum non-toxic concentration of an aqueous extract of rhizoma osmundae on the CIK cells is 1200 [mu] g / mL, and the inhibition of the aqueous extract of rhizoma osmundae on the survival rate of a grazing body of ichthyophthiriasis has dose correlation. When the concentration of the medicine reaches 100 mg / L (mu g / mL), the death rate of the grazing body reaches 100%, and in addition, calculation shows that the median lethal concentration (LD50) of the rhizoma osmundae aqueous extract to the ichthyophthirius multifiliis grazing body is 13.256 mg / L. The application of the rhizoma osmundae in inhibiting the survival rate of the ichthyophthirius multifiliis of the fish under the cell non-toxic concentration is provided for the first time, a novel medicine is provided for preventing and treating ichthyophthirius multifiliis infection of aquaculture animals, and the rhizoma osmundae is a natural medicinal plant, is environmentally friendly and has the advantages of reducing the risk of medicine residues and the like.

The invention discloses an early warning factor detection method for SAA-ichthyophthirius multifiliis infection, and particularly relates to the technical field of SAA-ichthyophthirius multifiliis infection detection.The specific steps are as follows: S1, RNA extraction; s2, removing reverse transcription of the genome DNA and cDNA (complementary deoxyribonucleic acid); s3, constructing a cloning vector of the Saa CDS region; s4, constructing an Saa phylogenetic tree; s5, constructing a Saa fluorescent quantitative detection primer design and standard curve; s6, performing saa expression after the ichthyophthirius multifilis infects the pelteobagrus vachelli; and S7, data statistics and analysis. Pelteobagrus vachelli is taken as a research object, a saa CDS region of the pelteobagrus vachelli is cloned and analyzed, a saa fluorescent quantitative detection method is established, saa mRNA expression level is dynamically monitored at different stages of ichthyophthirius multifiliis infected by the pelteobagrus vachelli, the reaction time and part of the saa in the ichthyophthirius multifiliis infection are disclosed, and the application of the saa fluorescent quantitative detection method to the detection of the ichthyophthirius multifiliis infection is realized. A foundation is laid for function analysis and application research of saa, and a foundation is laid for early prevention and early control of ichthyophthiriasis.

The invention discloses a treatment device and a treatment method for ichthyophthiriasis of largemouth bass, and belongs to the technical field of aquaculture. The device comprises a treatment bin, a filtering bin and a medicine bin, a disinfecting and killing device is further arranged between the filtering bin and the medicine bin, and all the components cooperate to achieve water circulation, temperature control, salinity adjustment, medicine mixing and disinfecting and filtering. During treatment, the temperature of a water body is stable, the salinity is accurately adjusted through the salt adding box, the salinity is adjusted from high salinity to low salinity, then melia azedarach oil and eucalyptus oil with specific dosage are matched to kill ichthyophthirius multifiliis, and then povidone iodine is used for disinfection and vitamin C is used for assisting recovery. The method solves the problems of large drug dosage, high energy consumption, inaccurate salinity adjustment and easy disease recurrence in the prior art, has the advantages of drug and energy consumption saving, accurate salinity adjustment, thorough treatment and simple and convenient operation, and is suitable for efficient treatment of industrial largemouth bass ichthyophthiriasis.

This invention discloses the application of Terminalia chebula in the prevention and treatment of Ichthyophthirius multifiliis (white spot disease) in aquatic animals. Toxicity experiments on grass carp kidney cells (CIK) showed that the maximum non-toxic concentration (NDC) of the aqueous extract of Terminalia chebula against CIK cells was 500 μg / mL. The inhibition of Ichthyophthirius multifiliis predator survival by the aqueous extract was dose-dependent, and the predator mortality rate reached 100% at a concentration of 100 mg / L (μg / mL). Furthermore, the median lethal concentration (LD50) of the aqueous extract against Ichthyophthirius multifiliis predators was calculated to be 14.577 mg / L. This invention is the first to propose the application of Terminalia chebula in inhibiting the survival rate of Ichthyophthirius multifiliis predators in fish at a non-toxic concentration to cells, providing a novel drug for the prevention and treatment of Ichthyophthirius multifiliis infection in aquatic animals. Terminalia chebula is a natural medicinal plant, environmentally friendly, and has the advantages of reducing the risk of drug residues.

The invention relates to the technical field of breeding and cultivation, in particular to a breeding device for preventing and treating ichthyophthirius multifiliformis, which comprises a fishpond body, the fishpond body is divided into three parts, namely a healthy pond, a transition pond and an infection pond, the side edges of the healthy pond and the infection pond are respectively provided with a filtering drainage system, and a classification assembly is arranged in the transition pond; and a medicine applying assembly is arranged in the infection pool. The fish pond body is divided into three parts, infected fishes and healthy fishes can be bred separately, the problem that a large amount of ichthyophthiriasis is spread and infected in the diptychus maculatus is avoided, secondary screening and classification in the diptychus maculatus breeding process are achieved through the arrangement of the classification assembly, and the breeding efficiency is improved. Therefore, complete separation of healthy diptychus maculatus and infected diptychus maculatus is ensured. Through the arrangement of the medicine applying assembly, the technical effect of autonomously applying medicine to infected diptychus maculatus for treatment is achieved, and the technical problem that in the prior art, diptychus maculatus cannot be autonomously classified and applied with medicine in the breeding process is solved.

The application discloses application of epigallocatechin gallate in prevention and treatment of ichthyophthiriasis, and belongs to the technical field of fish disease prevention and control. The application first applies epigallocatechin gallate to prevention and treatment of ichthyophthiriasis, and can achieve 100% killing of the worm body in an effective dose range, and the 96-hour median lethal concentration of goldfish is 63.7 mg / L, which is 6.3-212.3 times of the effective anti-worm concentration (0.3-10.0 mg / L) in vitro, and is 15.9-31.9 times of the effective anti-worm concentration (2-4 mg / L) in vivo, and is safe to fish in the effective range of killing the worm. The epigallocatechin gallate is derived from a natural plant, has low toxicity to human and animals, and has the potential to be developed into a new green alternative drug for prevention and treatment of ichthyophthiriasis.

The invention discloses an application of a surface layer protein serving as a target in screening a medicine for killing in-vitro parasitic ciliates of fishes. Through virtual screening, a molecular dynamics simulation technology, in-vitro insecticidal effect detection and a pull-down experiment of recombinant surface layer protein and Ichthyophthirius multifiliis total protein, the surface layer protein structure of Ichthyophthirius multifiliis and the drug target characteristics of the surface layer protein structure of Ichthyophthirius multifiliis are disclosed; potential ichthyophthirius multifilis-resistant drug molecules including xanthohumol, magnolol and honokiol are screened by taking the compound as a target spot, and insecticidal experiments prove that the three compounds are natural active compounds for killing parasitic ciliates. The xanthohumol is applied to the preparation of the medicine for killing the fish ectoparasites for the first time, especially for killing ichthyophthirius multifilis, the parasites can be killed at the effective dose of 100%, and the xanthohumol has a remarkable prevention and treatment effect on ichthyophthiriasis. The xanthohumol is an isopentene flavonoid compound, is separated from humulus lupulus, is of great significance to treatment of ichthyophthiriasis, and has a wide application prospect.

The invention relates to a production method of retention type ichthyophthiriasis immune fry of Malus salicina, and belongs to the technical field of aquaculture. The technical problem that a large number of fishes or all fishes die due to the fact that retention type Malus salmonilio is extremely prone to being infected with ichthyophthiriasis under the normal culture condition is solved. The preparation method comprises the following steps: firstly, putting healthy retention type margarus salicina fries into a pond, and feeding the healthy retention type margarus salicina fries; then putting fish fries of the retention type Malus salicina infected with ichthyophthiriasis, and feeding the fish fries of the retention type Malus salicina; then the salinity of the pond is adjusted to obtain cured retention type Malus salicina, and the cured retention type Malus salicina is the ichthyophthiriasis immune fry of the retention type Malus salicina. The retention type ichthyophthiriasis immune offspring seed of the Malus salicina obtains an antibody for resisting ichthyophthiriasis infection, so that the offspring seed cannot be infected with ichthyophthiriasis.

The application provides a vaccine against ichthyophthiriasis and a preparation method and application thereof, and belongs to the technical field of fish disease prevention and control. The application uses inactivated whole worms of in-vitro large-scale cultured Amphilina alata as a cross antigen, and fish complement C5a protein as an immunoadjuvant, and the two synergistically play a high-efficiency immune protection role. The application mixes and emulsifies the C5a adjuvant and the antigen at an effective dose of 1x10 4 6x10 4 per tail to prepare the vaccine. After intraperitoneal injection and secondary boosting, the vaccine produces significant cross protection against the infection of the ichthyophthirius, and the protection effect is significantly better than that of a traditional Freund's adjuvant vaccine. The application breaks the bottleneck that the ichthyophthirius cannot be cultured in vitro and thus a large number of worm bodies cannot be obtained for large-scale preparation of whole worm vaccines, and also solves the problem of poor safety of traditional adjuvants, has the advantages of low cost, good effect, easy large-scale production and the like, and is suitable for green immune prevention and control of ichthyophthiriasis of freshwater fish.

The utility model discloses an ichthyophthirius multifiliis ecological prevention and control device which comprises a cylinder body, a water inlet assembly and a water drainage assembly, a water outlet of the water inlet assembly is arranged in the cylinder body and arranged in the tangential direction with the axis of the cylinder body as the circumference, and a water inlet of the water drainage assembly is connected to the middle of the bottom of the cylinder body; the ashtray further comprises an illumination assembly and a shading assembly, the illumination assembly is used for providing illumination for the interior of the ashtray body, the shading assembly comprises a light shield and a lifting mechanism, the lifting mechanism can lift the light shield, and when the light shield covers the periphery of the ashtray body, a light-free environment is provided for the ashtray body. The ichthyophthirius multifiliis ecological control device can effectively reduce the number of ichthyophthirius multifiliis and improve the disease treatment effect of the ichthyophthirius multifiliis.

The invention discloses application of glycyrrhetinic acid in preparation of drugs for killing fish parasites, and belongs to the technical field of prevention and control of aquatic animal diseases. The EC50 value of the glycyrrhetinic acid for killing the grazing body of the ichthyophthirius multifilis is 0.672 mg / L, and the EC50 value of the glycyrrhetinic acid for killing the cyst of the ichthyophthirius multifilis is 1.508 mg / L. The glycyrrhetinic acid kills ichthyophthirius multifilis through soaking administration or oral administration, the concentration of the glycyrrhetinic acid in soaking administration is 1.5-4 mg / L, and the feeding amount of the glycyrrhetinic acid in oral administration is not lower than 90 mg / kg.d <-1 >. The glycyrrhetinic acid can kill grazing bodies and cysts of the ichthyophthirius multifilis, reduce the fecundity of the ichthyophthirius multifilis and improve the survival rate of fishes, and is a green, safe and efficient medicine for preventing and treating ichthyophthiriasis.