6 results about "Ichthyophthiriasis" patented technology

The invention discloses application of ursolic acid in resisting fish ichthyophthiriasis. The ursolic acid has a remarkable killing effect on ichthyophthirius multifilis in an in-vitro stage, and the 4-hour half effective insecticidal concentration (4h-EC50) of the ursolic acid on a grazing body is 0.3774 mg / L; and the 22-hour half effective insecticidal concentration (22h-EC 50) on the capsule is 4.931 mg / L. An acute toxicity test shows that the 96-hour median lethal concentration (96h-LC50) of ursolic acid to goldfish is 9.358 mg / L, the therapeutic index (TI = 96h-LC50 / 4h-EC50) is up to 24.8, and the safety of ursolic acid is remarkably superior to that of a traditional medicine. Ursolic acid used in the invention is a plant-derived active component, has the characteristics of high environmental compatibility and low biological accumulation, meets the development requirements of green fishery, and has an industrialization basis for being developed into eco-friendly aquatic products.

The invention discloses a method for preventing and treating ichthyophthiriasis of industrially cultured snakehead fries, which belongs to the technical field of snakehead culture and comprises the following steps: completely catching snakehead fries infected with ichthyophthiriasis in a culture pond, and transferring the snakehead fries into a biological bait culture pond for isolated culture; transferring the snakeheads subjected to isolated culture to a culture pond, and raising the salinity of the culture pond for culture; in the same period of culturing in the culture pond, the composite Chinese herbal medicine preparation is splashed into the culture pond to conduct soaking treatment on the snakeheads; after treatment is finished, in daily culture in a culture pond, a red-knees herb decoction water solution is splashed into the culture pond to consolidate the treatment effect of the snakehead and prevent the snakehead. Through the synergistic effect of sick fish isolation, salinity regulation and control and red-knees herb treatment, the survival rate and quality of fish culture are remarkably improved, the culture yield is increased, economic benefits are improved, ecological benefits are improved, and powerful support is provided for green development of factory fish culture.

The invention discloses application of rhizoma osmundae in prevention and treatment of ichthyophthiriasis of aquaculture animals, toxicity experiments of rhizoma osmundae on grass carp kidney cells (CIK) show that the maximum non-toxic concentration of an aqueous extract of rhizoma osmundae on the CIK cells is 1200 [mu] g / mL, and the inhibition of the aqueous extract of rhizoma osmundae on the survival rate of a grazing body of ichthyophthiriasis has dose correlation. When the concentration of the medicine reaches 100 mg / L (mu g / mL), the death rate of the grazing body reaches 100%, and in addition, calculation shows that the median lethal concentration (LD50) of the rhizoma osmundae aqueous extract to the ichthyophthirius multifiliis grazing body is 13.256 mg / L. The application of the rhizoma osmundae in inhibiting the survival rate of the ichthyophthirius multifiliis of the fish under the cell non-toxic concentration is provided for the first time, a novel medicine is provided for preventing and treating ichthyophthirius multifiliis infection of aquaculture animals, and the rhizoma osmundae is a natural medicinal plant, is environmentally friendly and has the advantages of reducing the risk of medicine residues and the like.

The invention discloses a treatment device and a treatment method for ichthyophthiriasis of largemouth bass, and belongs to the technical field of aquaculture. The device comprises a treatment bin, a filtering bin and a medicine bin, a disinfecting and killing device is further arranged between the filtering bin and the medicine bin, and all the components cooperate to achieve water circulation, temperature control, salinity adjustment, medicine mixing and disinfecting and filtering. During treatment, the temperature of a water body is stable, the salinity is accurately adjusted through the salt adding box, the salinity is adjusted from high salinity to low salinity, then melia azedarach oil and eucalyptus oil with specific dosage are matched to kill ichthyophthirius multifiliis, and then povidone iodine is used for disinfection and vitamin C is used for assisting recovery. The method solves the problems of large drug dosage, high energy consumption, inaccurate salinity adjustment and easy disease recurrence in the prior art, has the advantages of drug and energy consumption saving, accurate salinity adjustment, thorough treatment and simple and convenient operation, and is suitable for efficient treatment of industrial largemouth bass ichthyophthiriasis.

This invention discloses the application of Terminalia chebula in the prevention and treatment of Ichthyophthirius multifiliis (white spot disease) in aquatic animals. Toxicity experiments on grass carp kidney cells (CIK) showed that the maximum non-toxic concentration (NDC) of the aqueous extract of Terminalia chebula against CIK cells was 500 μg / mL. The inhibition of Ichthyophthirius multifiliis predator survival by the aqueous extract was dose-dependent, and the predator mortality rate reached 100% at a concentration of 100 mg / L (μg / mL). Furthermore, the median lethal concentration (LD50) of the aqueous extract against Ichthyophthirius multifiliis predators was calculated to be 14.577 mg / L. This invention is the first to propose the application of Terminalia chebula in inhibiting the survival rate of Ichthyophthirius multifiliis predators in fish at a non-toxic concentration to cells, providing a novel drug for the prevention and treatment of Ichthyophthirius multifiliis infection in aquatic animals. Terminalia chebula is a natural medicinal plant, environmentally friendly, and has the advantages of reducing the risk of drug residues.

The application provides a vaccine against ichthyophthiriasis and a preparation method and application thereof, and belongs to the technical field of fish disease prevention and control. The application uses inactivated whole worms of in-vitro large-scale cultured Amphilina alata as a cross antigen, and fish complement C5a protein as an immunoadjuvant, and the two synergistically play a high-efficiency immune protection role. The application mixes and emulsifies the C5a adjuvant and the antigen at an effective dose of 1x10 4 6x10 4 per tail to prepare the vaccine. After intraperitoneal injection and secondary boosting, the vaccine produces significant cross protection against the infection of the ichthyophthirius, and the protection effect is significantly better than that of a traditional Freund's adjuvant vaccine. The application breaks the bottleneck that the ichthyophthirius cannot be cultured in vitro and thus a large number of worm bodies cannot be obtained for large-scale preparation of whole worm vaccines, and also solves the problem of poor safety of traditional adjuvants, has the advantages of low cost, good effect, easy large-scale production and the like, and is suitable for green immune prevention and control of ichthyophthiriasis of freshwater fish.