The invention relates to the technical field of pesticides for preventing and treating diseases of aquatic animals, which particularly discloses a pesticide for preventing and treating ichthyophthiriasis of pet fish. The pesticide consists of sodium chloride, sodium bicarbonate, magnesium chloride, formaldehyde, polyiodide, potassium permanganate, carbazochrome sodium sulfonate, copper sulfate, vitamin K1, ferrous sulfate and water in balance, wherein the concentration of sodium chloride is 15-25g/L, the concentration of sodium bicarbonate is 4.6-5.4g/L, the concentration of magnesium chloride is 0.12-0.25g/L, the concentration of formaldehyde is 20-40mg/L, the concentration of polyiodide is 6-15mg/L, the concentration of potassium permanganate is 1.6-3.0mg/L, the concentration of carbazochrome sodium sulfonate is 1.2-2.0mg/L, the concentration of copper sulfate is 0.05-0.15mg/L, the concentration of vitamin K1 is 0.6-1.0mg/L, and the concentration of ferrous sulfate is 0.02-0.07mg/L.The pesticide provided by the invention can efficiently prevent and treat ichthyophthiriasis of pet fish, guarantee healthy development of pet fish cultivation, and has extensive market prospect and economic benefit.