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Canine parvovirus resistant refined antibody and preparation method thereof

The invention discloses a canine parvovirus resistant refined antibody and a preparation method thereof. The preparation method comprises the following steps: S1, preparing canine parvovirus inactivated vaccines by taking canine parvovirus CPV-S5 and CPV-1401 separated and identified in Guangdong regions as basic strains; S2, preparing canine parvovirus hyper-immune serum from vaccine immunity healthy dogs; S3, separating canine parvovirus resistant immune globulin in the serum prepared in the S2 through saturated ammonium sulfate stepped salting-out; S4, purifying, namely desalting the canine parvovirus resistant immune globulin extracted in the step S3 through dextrangel; S5, performing ultrafiltration concentration on the canine parvovirus resistant immune globulin subjected to desalting treatment in the step S4, filtering to remove bacteria, and performing split charging, thereby obtaining the product. According to the canine parvovirus resistant refined antibody prepared by the invention, the protein content reaches 134mg/ml, and the hemagglutination inhibition titer is not lower than 1:10240; and the canine parvovirus resistant refined antibody can be applied to treatment and emergency prevention when dogs of different varieties are in contact infection with the canine parvovirus, is capable of increasing the organism immunity and resistance of ill dogs, does not cause any adverse reaction, does not have any clinical side effect, has high safety and has excellent popularization and application prospects.
Owner:SOUTH CHINA AGRI UNIV +1

Rhodiola rosea tea and preparation method thereof

The present invention discloses rhodiola rosea tea and a preparation method thereof, belongs to human use health-care food, and especially relates to health-care drink prepared from rhodiola rosea, crocus sativus and highland barley as main raw materials and a preparation method thereof. The rhodiola rosea tea is prepared from the following active ingredient raw materials: rhodiola rosea, crocus sativus, highland barley, highland radix acanthopanacis semticosi, rhizoma gymnadeniae, siraitia grosvenorii, panax ginseng fruits, radix astragali, licorice and brick tea. The preparation method comprises the following steps: 1) the rhodiola rosea, highland radix acanthopanacis semticosi, siraitia grosvenorii, radix astragali and licorice are boiled and extracted, the extractum is dried, and the dried extractum is crushed for standby application; 2) the highland barley is cooked, the cooked highland barley is dried, the dried highland barley is stir-fried, and the stir-fried highland barley is crushed into fine powder for standby application; 3) the crocus sativus, rhizoma gymnadeniae, panax ginseng fruits and brick tea are crushed for standby application; and 4) the prepared raw materials are mixed evenly, the mixture is granulated, the granulated mixture is pressed into round or diamond-shaped tablets, and the tablets are dried to obtain the finished products (or the mixture is prepared into drink by adding pure water, the drink is sterilized, and the sterilized drink is canned or bottled to obtain the finished products). The advantages of the rhodiola rosea tea are that the health-care drink is reasonable and scientific in matching of active ingredient raw materials during preparation and rich in nutrients, contains many human body essential beneficial substances, can prevent fatigue, supplement qi and activate blood circulation, and regulate blood lipid function, and is particularly suitable for drinking of population who are lack of energy, easily fatigue, high in blood lipids and withered in facial features.
Owner:CHENGDU SHENGHONGYUAN SCI & TRADING

Method for controlling cryptocaryon irritans diseases of fishes by using ozone

The invention belongs to the field of fish disease prevention and control and in particular relates to a method for controlling cryptocaryon irritans diseases of fishes by using ozone. According to the method, by virtue of the characteristic that larvae of cryptocaryon irritans are centrally incubated at night, ozone is introduced into a breeding water body in a larva incubation period, and the ozone concentration is kept within a lowest concentration range which is capable of effectively deactivating the larvae of the cryptocaryon irritans; and by cutting off the life history of the cryptocaryon irritans, the purposes of treating and preventing the cryptocaryon irritans diseases can be achieved. By adopting the method, experiments that the larvae of the cryptocaryon irritans are treated with seawater of different ozone concentrations show that the lowest effective deactivation concentration is 0.1-0.15mg / L; and trachinotus ovatus is adopted for effect verification experiments, resultsshow that 411.4+14.7 cysts are generated by each fish on average when the ozone concentration is 0mg / L, 0.4+ / -0.1 cyst is generated by each fish on average when the ozone concentration is 0.1mg / L, and 0 cyst is generated by each fish on average when the ozone concentration is 0.15mg / L. Results show that when the ozone concentration is 0.1-0.15mg / L, the larva infection capability of the cryptocaryon irritans can be remarkably reduced (P is less than 0.05), and the cryptocaryon irritans diseases can be effectively controlled.
Owner:SUN YAT SEN UNIV

Dairy cow mammitis staphylococcus aureus-Escherichia coli bigeminy polyvalent vaccine and preparation method thereof

The invention relates to a dairy cow mammitis staphylococcus aureus-Escherichia coli bigeminy polyvalent vaccine and a preparation method thereof. The vaccine comprises the following components in part by volume: 4 parts of antigens and 6 parts of Montanide ISA70MVG immune oil adjuvant, wherein the antigens comprise a, a combination of any two or more than two of antigens of thallus polysaccharide and protein mixtures of staphylococcus aureus subsp. aureus CGMCC No.4595, 4596, 4597 and 4598, b, a combination of any two or more than two of toxin antigens of CGMCC No.4595, 4596, 4597 and 4598, c, a thallus protein antigen of Escherichia coli CGMCC NO. 4594 and d, a toxin antigen of CGMCC No. 4594. The preparation method comprises the following steps of: preparing strains; preparing the thallus antigens; preparing the toxin antigens; and mixing the thallus antigens, the toxin antigens and the Montanide ISA70MVG immune oil adjuvant according to the volume ratio to obtain the vaccine. The invention has the advantages that: 1, the coverage area of vaccine strain antigens is wide, and the immunogenicity is high; 2, the effect is obvious; and 3, the safety of the vaccine is high.
Owner:新疆维吾尔自治区畜牧科学院兽医研究所

Traditional Chinese medicine and traditional Tibetan medicine composition for stomach warming and liver nourishing and method for preparing same

ActiveCN102614483BHas the effect of warming the stomach and nourishing the liverBloating disappearsPre-extraction tea treatmentDigestive systemCordyceps militarisBULK ACTIVE INGREDIENT
A traditional Chinese medicine and traditional Tibetan medicine composition for stomach warming and liver nourishing and a method for preparing the same belong to health care product combination, especially combination of health care products made of traditional Chinese medicine, and the method for preparing the combination. The composition is made of active ingredients including cordyceps militaris, sea-buckthorn, fructus amomi, highland barley malt, citrus chirocarpus, coriander root, tea leaves and the like. The traditional Chinese medicine and traditional Tibetan medicine composition can be made into stomach-warming and liver-nourishing products of different dosage forms, and has the advantages that the fructus amomi, rhizome zingiberis, the coriander root and the citrus chirocarpus are importantly used, and stomach warming and nourishing effects can be achieved; the cordyceps militaris and the sea-buckthorn have a liver nourishing function; and the citrus chirocarpus and pueraria lobata ohwi have functions of five organ warming and nourishing. With joint action of the medicines, stomach warming and liver nourishing effects can be achieved.
Owner:XIZANG WENSHENGJI TECH
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