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Inactivated vaccine for preventing and treating paramyxovirus disease of pigeon and its prepn

A technology for paramyxoviruses and inactivated vaccines, applied in antiviral agents, viral antigen components, pharmaceutical formulations, etc., can solve the problems of short immunity period and achieve the effects of easy absorption, good effect and convenient use

Inactive Publication Date: 2007-08-22
广东省家禽科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of chicken NDV vaccine to immunize pigeon groups generally cannot obtain satisfactory results, and can only obtain low-level protection, the protection rate is only about 20%, and the immunization period is short; while the inactivated PA / PMV-1 homologous virus is currently used Vaccine immunization can provide relatively long-lasting immune protection for immunized pigeons, but there are also immunized pigeon groups that still have PA / PMV-I disease

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Prepare the oil-emulsion adjuvant inactivated vaccine for preventing and treating pigeon type I paramyxovirus disease according to the following steps:

[0044] 1. Preparation of PA / PMV-I antigen

[0045] a) Cultivation of PA / PMV-I antigen: the pigeon type I paramyxovirus PA / PMV-I species poison GDP199902 CCTCC V200605 is diluted with sterile physiological saline 100 times, so that every 0.5ml poisonous amount is about 10 4.0～5.0 EID 50 10-11 day-old non-immune chicken embryos were inoculated into the allantoic cavity, 0.2ml per embryo, incubated at 37°C for 48-96 hours, and the dead embryo fluid was harvested after 24 hours, and the HA titer was measured, and the HA titer was taken as 2 7～9 chicken embryo liquid, sub-packaged, and stored at -15°C to -20°C for later use;

[0046] b) Inactivation of PA / PMV-I antigen: Add 2ml of formalin solution with a formaldehyde content of 37% in every 1000ml of chicken embryo liquid, and transfer it to another high-pressure sterili...

Embodiment 2

[0052] Prepare propolis adjuvant inactivated vaccine for preventing and controlling pigeon type I paramyxovirus disease according to the following steps:

[0053] 1. Preparation of PA / PMV-I antigen

[0054] a) Cultivation of PA / PMV-I antigen: the pigeon type I paramyxovirus PA / PMV-I species poison GDP19990 CCTCC V200605 is diluted with sterile physiological saline 100 times to make about 10 per 0.2ml of toxic content 4.0～5.0 EID 50 , the allantoic cavity was inoculated with 10-11 day-old non-immune chicken embryos, 0.2ml per embryo, incubated at 37°C for 48-96 hours, harvested the dead embryo fluid after 24 hours, and measured its HA titer, which was taken as 2 7～9 chicken embryo liquid, sub-packaged, and stored at -15°C to -20°C for later use;

[0055] b) Inactivation of PA / PMV-I antigen: Add 1ml to 3ml of formalin solution with a formaldehyde content of 40% in every 1000ml of chicken embryo liquid, and transfer it to another high-pressure sterilized bottle and put it on a ...

Embodiment 3

[0061] Prepare the pure Chinese medicine composite adjuvant inactivated vaccine for preventing and controlling pigeon type I paramyxovirus disease according to the following steps:

[0062] 1. Preparation of PA / PMV-I antigen

[0063] a) Cultivation of PA / PMV-I antigen: the pigeon type I paramyxovirus PA / PMV-I species poison GDP199902 CCTCC V200605 is diluted with sterile physiological saline 100 times, so that every 0.3ml poisonous amount is about 10 4.0～5.0 EID 50 10-11 day-old non-immune chicken embryos were inoculated into the allantoic cavity, 0.2ml per embryo, incubated at 37°C for 48-96 hours, and the dead embryo fluid was harvested after 24 hours, and the HA titer was measured, and the HA titer was taken as 2 7～9 chicken embryo liquid, sub-packaged, and stored at -15°C to -20°C for later use;

[0064] b) Inactivation of PA / PMV-I antigen: Add 2ml of formalin solution with a formaldehyde content of 37% in every 1000ml of chicken embryo liquid, and transfer it to another...

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Abstract

The present invention proposes one kind of inactivated vaccine for preventing and treating type-I paramyxovirus disease of pigeon and its preparation process. The inactivated vaccine for preventing and treating type-I paramyxovirus disease of pigeon is prepared through screening out type-I paramyxovirus PA / PMV-I spawn GDP199902 CCTCC V200605 with powerful immunogenicity and high protecting rate against epidemic strain, inoculating un-immunized chick embryo, extracting PA / PMV-I antigen from dead chick embryo fluid, inactivating PA / PMV-I antigen, and adding adjuvant. The vaccine is used for preventing and treating PA / PMV-I disease of pigeon, and is specific, safe and effective.

Description

technical field [0001] The invention relates to a vaccine for preventing and treating type I paramyxovirus disease of pigeons and a preparation method thereof. Background technique [0002] Pigeon poultry type I paramyxovirus (PA / PMV-I) disease, commonly known as pigeon plague, also known as pigeon Newcastle disease, is a kind of highly contacted, Septic acute infectious disease. Since it was discovered in some areas of my country in the early 1980s, it has generally occurred in various pigeon breeding areas in my country, and it has always been a disease with the highest frequency and the greatest harm in pigeon diseases. All pigeons can be infected with morbidity, and spread rapidly, and the morbidity rate is high, and the mortality rate is generally between 20% and 80%. Those who endure it are often forced to eliminate because of neurological symptoms, causing great economic losses. In addition, the pathogen of this disease and the pathogen of chicken Newcastle disease (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/17A61K39/39A61K47/44A61P31/14A61K36/481A61K35/64A61K35/644A61K47/06A61K47/26
Inventor 邹永新
Owner 广东省家禽科学研究所
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