Method for controlling cryptocaryon irritans diseases of fishes by using ozone

A technology that stimulates cryptonium disease and ozone, which can be used in applications, fish farming, climate change adaptation, etc., and can solve problems such as high cost, unsuitable for large-scale aquaculture production, and environmental pollution.

Inactive Publication Date: 2020-05-22
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some experts have studied the prevention and control effect of Chinese herbal medicines such as pepper, ginger, gallnut, and betel nut on stimulating Cryptocaryon, and achieved certain results. However, the current production cost is high and it is not suitable for large-scale breeding production
Therefore, the current methods commonly used to prevent and treat cryptocystosis are still frequent use of chemical drugs such as formaldehyde, CuSO4, and copper-iron mixture. Although chemical drugs can effectively control cryptocystosis, there are huge hidden dangers in practical applications, such as Fish toxicity, drug residues, environmental pollution, etc., so it is imminent to establish a pollution-free and efficient control method

Method used

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  • Method for controlling cryptocaryon irritans diseases of fishes by using ozone
  • Method for controlling cryptocaryon irritans diseases of fishes by using ozone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1: Stimulating Cryptocaryon larvae by using ozone treatment in vitro to determine the minimum lethal dose (MinimumLethal Dose, MLD)

[0011] The Cryptocaryon stimulator used in this experiment was the species passed down by the Guangdong Provincial Marine Fisheries Experimental Center in Daya Bay, Aotou Town, Huizhou City. The Cryptocaryon stimuli larvae that had just hatched within 2 hours before the experiment were collected and concentrated by centrifugation at 4000rpm for 5 minutes. After concentration, a few drops were taken and observed under a microscope. If there was no change in the swimming of the larvae, proceed to the next step. Use ozone generator to pass into ozone to seawater, the ozone concentration of seawater is adjusted to 0.02mg / L, 0.03mg / L, 0.04mg / L, 0.05mg / L, 0.1mg / L and 0.15mg / L. The seawater containing different concentrations of ozone was mixed with the larvae, and the results of the larvae treated with different concentrations of ozone ...

Embodiment 2

[0015] Embodiment two: verify insecticidal effect by using ozone in aquaculture system

[0016] The egg-shaped pomfret (21.4±0.7g / tail) used in this experiment was purchased from Daya Bay, Huizhou City, Guangdong Province. Before the experiment, they were domesticated in a round plastic barrel (volume 1 ton) for 2 weeks, raised in micro-flow water, fed commercial feed, and used natural seawater after sand filtration, with a salinity of 29PPT and a water temperature of 24±1°C.

[0017] The pompano ovata were randomly divided into 5 groups, 4 groups were treated with different concentrations of ozone, and 1 group was the control group, with 3 parallel fish in each group and 4 fish in each parallel. Before the experiment, ozone gas was introduced into the ozone treatment group, and the ozone concentration in seawater was measured using a dissolved ozone analyzer in water (Nobo, DOZ-7600), so that the ozone concentration in seawater reached 0mg / L, 0.05mg / L, 0.1mg / L and 0.15 mg / L r...

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Abstract

The invention belongs to the field of fish disease prevention and control and in particular relates to a method for controlling cryptocaryon irritans diseases of fishes by using ozone. According to the method, by virtue of the characteristic that larvae of cryptocaryon irritans are centrally incubated at night, ozone is introduced into a breeding water body in a larva incubation period, and the ozone concentration is kept within a lowest concentration range which is capable of effectively deactivating the larvae of the cryptocaryon irritans; and by cutting off the life history of the cryptocaryon irritans, the purposes of treating and preventing the cryptocaryon irritans diseases can be achieved. By adopting the method, experiments that the larvae of the cryptocaryon irritans are treated with seawater of different ozone concentrations show that the lowest effective deactivation concentration is 0.1-0.15mg / L; and trachinotus ovatus is adopted for effect verification experiments, resultsshow that 411.4+14.7 cysts are generated by each fish on average when the ozone concentration is 0mg / L, 0.4+ / -0.1 cyst is generated by each fish on average when the ozone concentration is 0.1mg / L, and 0 cyst is generated by each fish on average when the ozone concentration is 0.15mg / L. Results show that when the ozone concentration is 0.1-0.15mg / L, the larva infection capability of the cryptocaryon irritans can be remarkably reduced (P is less than 0.05), and the cryptocaryon irritans diseases can be effectively controlled.

Description

technical field [0001] The invention belongs to the field of fish disease prevention and control, and in particular relates to a method for controlling fish-stimulated cryptocystosis by using ozone. Background technique [0002] According to the characteristics of stimulating the larvae of Cryptocaryon irritans to have free swimming ability and the concentrated hatching of cysts at night (7:00 p.m. And achieve the minimum effective inactivation concentration to prevent larval infection, so as to achieve the purpose of controlling cryptocystosis. [0003] Ozone is an allotrope of oxygen with the molecular formula O 3 , normally light blue, gas with a special smell; Ozone is one of the strongest oxidants in nature, has a broad-spectrum bactericidal effect, its bactericidal efficiency is higher than that of chlorine, and its oxidation-reduction potential in water is second only to fluorine . Therefore, since ozone was identified and named by the French scientist Schonbein in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/13
CPCA01K61/13Y02A40/81
Inventor 李安兴鲁格凌
Owner SUN YAT SEN UNIV
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