38 results about "Larvacea" patented technology

The invention relates to a bait feeding mode suitable for northern oyster larva breeding work, and in the D-type larva period of northern oysters, mixed bait of chrysophyceae and chlorella is fed. The crassostrea gigas larvae are fed with the mixed bait of the chrysophyceae and the chlorella, so that the growth rate and the survival rate of the larvae are remarkably improved, and the breeding period is shortened.

The invention belongs to the field of aquaculture engineering, and particularly relates to an automatic identification and feeding shellfish larva cultivation system and method, and the system comprises a larva circulating water cultivation tank which is used for accommodating shellfish larvae and a circulating water body; the larva image acquisition device is used for acquiring larva image data and sending the larva image data to the control system; the peristaltic pump is used for receiving the control of the control system and quantitatively adding the bait algae liquid; the algae density sensor is used for collecting real-time microalgae concentration; the control system is used for receiving the image data of the larva image acquisition device and analyzing and acquiring the number of larvae and the shell length of the larvae; data of the algae density sensor is also received and converted into real-time microalgae concentration; meanwhile, according to the obtained data, the amount of bait algae liquid needing to be supplemented is calculated, the peristaltic pump is controlled to be started, and feeding is executed. The accurate feeding control system for the shellfish larvae is innovatively researched and developed, and the larva feeding amount is accurately controlled on the basis of the constructed accurate feeding preset model.

The utility model relates to the technical field of water pumps, in particular to a volute type water taking pump capable of preventing shell vegetables from being attached, an LED lamp strip is installed in a closed cavity defined by a square lamp groove in a water filtering box and the inner wall of a box body, and the shell vegetables are driven to grow, breed and move in the area through the phototaxis of the shell vegetables. Therefore, shell vegetables and larvae are prevented from entering the water filtering tank; furthermore, an ultraviolet lamp strip is mounted in a closed cavity formed by the annular lamp groove and the inner wall of the volute body, so that the shell vegetables and the larvae entering the volute body can be killed, the shell vegetables are prevented from being attached to the inner wall of the volute body or an impeller, and the larvae are also prevented from entering various pipelines at the rear end to grow; secondly, the water inlet electromagnetic valve and the water outlet electromagnetic valve can be closed, the buffer box is screwed off, the high-pressure cleaning assembly is started, the interior of the volute body is thoroughly cleaned once, and attached shell vegetables are washed away; and finally, the buffer box can be screwed off regularly, and the shell vegetables or other objects rushing into the buffer box can be poured out.

The application relates to the field of aquaculture, and particularly discloses a method for cultivating oyster larvae, which comprises the following steps: S1, fertilized eggs are developed into D-shaped larvae in seawater at 22 DEG C for 20-24 hours; S2, the D-shaped larvae are cultivated under the condition that the temperature is 22-24 DEG C and the salinity is 29-31 ‰, feed and growth nutrient solution are fed daily, water is changed daily, after the D-shaped larvae appear eye spots, a larval collector is put in, and algal feed and growth promoting solution are fed daily, the larvae are cultivated into 0.8-1 mm juvenile shellfish, and are transferred to sea cultivation; the growth nutrient solution comprises the following components in weight parts: 1-2 parts of a small molecule peptide solution, 0.01-0.03 parts of neomycin sulfate powder, 0.5-1 part of corn starch, 2-4 parts of modified montmorillonite, and 10-15 parts of water. The method for cultivating oyster larvae has the advantages that the survival rate of the larvae is improved, the growth speed is accelerated, and the metamorphosis and attachment rate is improved.

The invention discloses a method for enhancing adhesion ability of coral larvae after enhancement and release. According to the method, coral larvae (such as coral plankton larvae) are exposed to a 40 mM calcium chloride (CaCl2) solution to be treated for 12-24 hours, and then enhancement and release are carried out. The treated larvae show remarkably enhanced adhesion ability after releasing, the accumulated adhesion rate within 3 days reaches 66.33% and is increased by 240% compared with that of a control group in the same period, and the average survival rate reaches 98.14%. Meanwhile, the transverse growth area and the budding number of attached larvae are remarkably increased, and the density of zooxanthellae, the photosynthetic efficiency and the chlorophyll content have no negative effects. The CaCl2 solution is used for successfully inducing the continuous attachment effect of the coral larvae, the method is easy and convenient to operate, low in cost and environmentally friendly, the attachment success rate of enhancement and release of the coral larvae and the subsequent growth performance of the larvae are remarkably increased, and the method is suitable for repairing and protecting a coral reef ecological system.

The invention discloses a method for efficiently producing triploid oysters, and belongs to the technical field of aquaculture breeding. The method comprises the following steps: S1, obtaining sperms and ova of diploid oysters, and carrying out sperm-ova mixed fertilization; s2, performing induction treatment on the fertilized eggs to inhibit release of a second polar body; s3, collecting fertilized eggs subjected to induction treatment, cleaning, and hatching to a larva stage; s4, 24-72 hours after fertilization, screening based on larva individual size differences, and collecting a target larva population; and S5, culturing the larvae screened in the step S4. According to the method provided by the invention, even if the initial induction rate is only about 70%, the larva population with the triploid rate higher than 90% can be stably obtained finally, the hatching rate and the survival rate are high, the method is easy and convenient to operate, and the requirement for induction conditions is low; the method is suitable for triploid preparation of precious oyster varieties in which a large number of fertilized eggs are difficult to obtain under strong induction conditions or oyster strains with important economic characters.

The invention discloses a shellfish larva vitality measuring device, and relates to the field of marine bivalve shellfish larva behavioristics, a measuring device is arranged in a heat preservation device, swimming tracks and swimming speeds of shellfish larvae in different temperature environments are simulated through the heat preservation device, and the measuring device comprises a measuring chamber, a camera and a measuring scale, a measuring ruler is arranged on the inner side wall of the measuring chamber, the camera faces the measuring chamber and is arranged on the opposite side of the measuring ruler, the camera records the motion trail of the shellfish larvae in the measuring chamber in the background of the measuring ruler, and the clearness of the motion trail of the shellfish larvae in the video is increased through irradiation of the light source. The swimming speed of the shellfish larvae is calculated by combining a measuring scale in a video, so that the accuracy of shellfish larva vitality evaluation is improved; meanwhile, the invention further discloses a determination method applying the shellfish larva vitality determination device, and the vitality strength of the shellfish larvae under the actual condition is judged.

The utility model discloses an integrated device for indoor induction of young coral reproduction and larva collection. The integrated device comprises a main body frame, a seawater circulating system and a high-salt titration system, the main body frame adopts a layered structure design, and the space is divided into an upper layer functional area and a lower layer functional area through an isolation frame; the seawater circulating system consists of a circulating water pool, an efficient water pump, an upper water pipe, an induction cylinder, a collecting tank and a lower water pipe; the high-salt titration system is connected with the induction cylinder and is used for accurately regulating and controlling the salinity of the water body. According to the utility model, 1) a modular design is adopted, and functions of temporary culture of coral parent, induction of larva discharge, collection of larvae and the like are integrated into a whole; dynamic adjustment of seawater salinity is achieved through a high-precision salinity titration system, environmental parameters in the induction cylinder can be accurately controlled through cooperation with a temperature control device so as to induce coral larva discharge, an overflow structure is adopted, the water level in the induction cylinder rises, larvae can be collected into a collecting barrel through an overflow opening, and efficient collection of the larvae is achieved.

The present invention discloses a method for cryopreservation of sperm of dwarf clams by vitrification, which relates to the field of marine biotechnology. The preservation method comprises the following steps: (1) taking dwarf clams in the breeding period, subjecting them to air-drying and temperature-changing stimulation in sequence to make them naturally discharge sperm, filtering and centrifuging to obtain high-density sperm, and storing it on ice; (2) mixing the high-density sperm obtained in step (1) with a cryoprotectant solution and placing it on ice to obtain a mixed sperm solution; the cryoprotectant solution is composed of dimethyl sulfoxide, coenzyme Q10 and polysucrose; (3) transferring the mixed sperm solution into a cryotube and directly immersing it in liquid nitrogen for storage. It has been found through research that after thawing at a temperature of 50-60 °C for 60-64 s, the D-shaped larva rate reaches more than 80%, realizing a cryopreservation method of directly putting the sample into liquid nitrogen for long-term storage.

The invention relates to the technical field of water environment monitoring, and discloses a water environment monitoring device which comprises a floating body, a water quality sensor and a lifting mechanism. The lifting mechanism is arranged on the floating body and used for driving the water quality sensor to descend to the preset depth of seawater or ascend; after reaching the preset depth, the water quality sensor is in contact with seawater for water quality monitoring; the cleaning assembly comprises an anode bar and a cathode tube which are located at the bottom end of the water quality sensor and concentrically arranged inside and outside and used for cleaning the outer surface of the water quality sensor. The hypochlorous acid diffuses to the surface of the water quality sensor, and because the hypochlorous acid is extremely thin and has strong oxidizing property, the hypochlorous acid can destroy cell walls of planktons and larvae, interfere neuromuscular functions of the planktons and the larvae and effectively inhibit key physiological processes of the planktons and the larvae in metabolism and early growth periods; therefore, larvae and spores of organisms such as algae and shellfish are inhibited from settling and growing on the surface of the water quality sensor.

The present application belongs to the technical field of aquaculture, and particularly relates to a method for improving survival rate of Dendostrea folium shell top larvae by dry exposure treatment. In the present application, the Dendostrea folium larvae in the late shell top stage are subjected to dry exposure stress treatment, and then rehydrated, fed and cultivated. Compared with the control group of oyster larvae without dry exposure stress treatment, the survival rate of the larvae cultivated by the shell top larvae subjected to dry exposure stress treatment is significantly improved under high temperature, low temperature and low oxygen stress. The present application can significantly improve the environmental stress resistance of Dendostrea folium larvae, and has a good application prospect in shellfish seed production.

The application relates to a breeding method of robust oysters, belonging to the field of animal genetics and breeding, which comprises population selection, energy recovery of larvae and selection of robust oysters; the selection of the robust oysters is carried out by selecting larvae with strong phototaxis and strong swimming ability by using the phototaxis of the oyster larvae. The robust oysters with the advantages of fast growth speed, large individual, fast attachment rate, disease resistance and stress resistance can be bred by selecting the individuals with strong phototaxis.

The present application relates to a kind of bait feeding mode suitable for northern oyster larva breeding work, at the D type larva period of northern oyster, feed mixed bait of chrysomonad and chlorella.The present application significantly improves the growth rate and survival rate of long oyster larvae by feeding mixed bait of chrysomonad and chlorella, and shortens the breeding cycle.

The invention discloses a method for inducing coral larva discharge and a high-adhesion-rate larva culture method. And slowly adding the high-salinity seawater into the seawater containing the coral cervi, and inducing the coral cervi to discharge the plantlet larvae. According to the method, high-salinity seawater is used for inducing the coral antler to discharge the planula larvae, so that the damage of a long-time high-temperature induction method to the coral parent and symbiotic zooxanthellae is remarkably reduced, the risks of whitening and death of the coral are avoided, and the survival rate and the recovery speed of the coral parent are increased.

The application discloses a kind of high-efficiency promoting coral larvae attachment metamorphosis and the growth of complex microbial inoculant and application thereof.The bacterium agent comprises Phytobacter sp.SCSIO43973 and Salipiger sp.SCSIO43974 two strains, and preservation numbers are respectively: GDMCC NO:65583 and 65650.The two strains are isolated from healthy antler cup-shaped coral.It is shown that the addition of these strains can significantly improve the attachment rate, metamorphosis rate of planulae, and the survival rate and budding rate of coral larvae.These characteristics show that the bacterium agent has the potential to improve the attachment metamorphosis rate of coral larvae and promote the growth in the field coral reef ecosystem, and has important significance for increasing the amount of larvae supplement, promoting the recovery and sustainable development of coral reef ecosystem.The above, the microbial inoculant of the application promotes the attachment metamorphosis of coral larvae and the growth of larvae, and provides a new technical means for the recovery of coral reef ecosystem.

The present application relates to a breeding method of hybrid seed of Babylonia formosae and Babylonia areolata. The breeding method comprises the following steps: breeding Babylonia formosae parent snails and Babylonia areolata parent snails in the dark, feeding 3-5% of the body weight of Babylonia formosae with vegetables, and feeding 10-15% of the body weight of Babylonia formosae with mixed biological bait, continuously feeding until the gonad matures, and feeding at least once a day; obtaining egg sac clusters by hybridizing Babylonia formosae parent snails and Babylonia areolata parent snails; hatching the egg sac clusters to form planktonic larvae, cultivating the planktonic larvae for 15-20 days, and metamorphosing the planktonic larvae into juvenile snails to obtain hybrid seed. The hybrid seed obtained by the breeding method has excellent metamorphosis rate, fertilization rate and hatching rate, and the average body weight, average shell length and survival rate are also significantly improved.

The invention belongs to the technical field of aquaculture, and particularly relates to a method for improving the survival rate of crassostrea gigas shell top larvae through dry exposure treatment. According to the method, crassostrea gigas larvae in the later stage of shell top are subjected to exposure stress treatment, and then rehydration, feeding and cultivation are conducted. Compared with control group oyster larvae which are not subjected to exposure stress treatment, the survival rate of the larvae cultured by utilizing the shell top larvae subjected to exposure stress treatment under high-temperature, low-temperature and low-oxygen stress is remarkably improved. According to the method, the environmental stress resistance of the crassostrea gigas larvae can be remarkably improved, and the method has a good application prospect in shellfish larva production.

To provide an aquarium capable of increasing the number of larval fish raised (production efficiency) while reducing the occurrence of abnormalities (dislocation, deformity, etc.) in the larval fish. [Solution] An aquarium for raising larvae has a flat section extending laterally, a pair of curved sections connected to one and the other horizontal ends of the flat section and bulging out in opposite directions in the horizontal direction and extending upward, and a pair of side sections connected to each of the pair of curved sections and extending upward. When the horizontal distance between the upper ends of the pair of side sections is W (mm) and the height from the flat section to the upper ends of the pair of side sections is H (mm), the relationship 1.12≦W / H≦1.90 is satisfied.

The present invention discloses a method for intelligent detection of bivalve planktonic larvae based on deep learning, comprising: collecting microscopic images of bivalve planktonic larvae with ink dots, image preprocessing, preparation of a planktonic larvae data set, training and acquisition of an application model, and planktonic larvae detection. The method uses a deep learning method to achieve intelligent detection of the number and shell length of bivalve planktonic larvae under a microscopic field of view, thereby reducing the workload and working time of manual microscopic observation of larvae, and greatly improving the work efficiency of larvae observation in the aquatic field.

The invention discloses an artificial breeding method for echinococcal snails, which is characterized in that young echinococcal snails are bred in a land-based environment through technical links of parent collection, parent domestication, mating pairing, embryonic development in oocysts, larva culture, filial generation breeding and the like, and a technical guarantee is provided for resource increase and breeding potential evaluation in the future. According to the method, a set of artificial breeding technical system is constructed by combining the physiological habits and breeding characteristics of the echinococcal snails (thousand hand snails) in a natural environment. The method has the characteristics of simple operation and easy popularization. According to the method, the international dispute of the artificial breeding technology of the spinous snails is solved, the domestic blank is filled up, and the field of China is promoted to reach the international leading level.

The invention relates to a crossbreeding method for babylonia lutosa and babylonia taiwan. The cross breeding method comprises the following steps: putting Babylonia taiwanensis and Babylonia lutosa into independent units in a dark environment for breeding, hybridizing, collecting oocysts, disinfecting the oocysts, and hatching to obtain Babylonia lutosa planktonic larvae; a fertilizer water agent and a nutritional agent are applied to culture water of babylonia planktonic larvae, unicellular algae are mixed for culture, and the babylonia planktonic larvae are metamorphosed and attached to form juvenile snails. Compared with the existing Babylonia taiwanensis and Babylonia lutosa, the Babylonia lutosa hybrid fry bred by the crossbreeding method not only has more excellent metamorphosis rate, but also has significantly improved fertilization rate, egg laying amount, hatching rate and larva survival rate.

The invention discloses an enhanced YOLO-based artificial cultivation shellfish larva health state early warning method, and belongs to the field of ocean and biological breeding, and the method comprises the steps: obtaining a shellfish larva image, carrying out the preprocessing of the shellfish larva image, and obtaining a preprocessing data set; optimizing the YOLOv8 model based on a CG module and a context self-attention mechanism, and constructing a deep learning model; training the deep learning model through the preprocessed data set to obtain a larva health state recognition model; wherein a loss function of the deep learning model is an Inner-CIoU loss function; and identifying the health state of the larva based on the larva health state identification model. According to the method provided by the invention, the shellfish larvae can be effectively identified, the health states of the larvae can be accurately classified, and a feasible solution is provided for the health state early warning task of artificially cultivated shellfish larvae.

The invention discloses an efficient preparation method of marine pearl oyster larvae. Through the technical links of gamete maturity evaluation, gonad mucus removal, ovum maturation promotion and activation, sperm motility excitation, excessive sperm fertilization and the like, the hatching rate of 90% or above is obtained, and therefore the purpose of efficient larva preparation is achieved. The method is different from the traditional method of dissecting mixed sperms and eggs for filtering, placing the sperms and eggs in a hatching container for settling for 1 hour, then pouring out supernate and directly scattering the sperms and eggs settled to the bottom into a pool for hatching, the solidification cognition of traditional marine pearl oyster breeding is subverted, the defects of low hatching rate, poor stability and the like of the traditional method are overcome, a large number of high-quality larvae are obtained, and the breeding efficiency is improved. And a technical guarantee is provided for high-efficiency production of offspring seeds, heterosexual pairing breeding and creation of excellent strains in the future. The method has the advantages of simplicity and convenience in operation, high repetition rate, high practicability and the like.

The invention belongs to the technical field of aquaculture breeding, and discloses a cultivation method of a new oligotrophic oyster strain. The method comprises the following steps: constructing an oyster larva mixed population with the same genetic background; when the larvae develop to a shell top larva stage, starvation stress treatment is started, the starvation stress treatment is accurate limited bait based on feeding rate feedback, and feeding is performed by halving the feeding rate; after the hunger stress treatment is finished, screening the larvae by using a screen, and dividing the individuals which are retained in the screen and have the shell length of more than or equal to 180 microns into oligotrophic resistance groups; and continuing to cultivate the screened oligotrophic group, so as to obtain the oligotrophic oyster strain. According to the method, the core breeding period is revolutionarily shortened to the day level from the month level, and the oligotrophic oyster strain bred through the method has the advantages that the higher bait conversion rate is achieved in the larva stage, and the genetic advantage can be stably expressed in the whole growth period.

The application discloses a preparation method and application of efficiently obtaining solar long-spined sea star larvae and juveniles / larvae. The method comprises the following steps: distinguishing the development period and the male and female genders of gonads; injecting oxytocin, separately collecting eggs and sperms, adding active sperms in batches after the eggs are broken, and the quantity ratio of the sperms and the eggs is 50-100:1; controlling the temperature, salinity and pH during the hatching of the larvae, culturing under full or half light shielding, and stirring when the fertilized eggs gather; feeding the hatched larvae with small new moon-shaped algae, changing water and removing dead and abnormal individuals; increasing the amount of feed when the larvae develop into brachiolaria, adding an attachment base when 70% of the planktonic larvae develop into late-stage brachiolaria with a size of 1000mu m*600mu m, transferring the juveniles to living stones with benthic coral algae after metamorphosis, and culturing until the long-spined sea star larvae are harvested. The application significantly improves the survival rate and metamorphosis rate of the planktonic larvae, and efficiently obtains a batch of larvae and juveniles / larvae in a short time.

The present invention proposes a biofilm-based high-salt culture method for whiteleg shrimp, which is characterized by comprising the following steps: adding a biological filler to a culture pond once when the whiteleg shrimp is in the flea-like larval stage to increase the salinity to 20‰-25‰; adding the biological filler to the culture pond a second time in the mysid stage to increase the salinity to 40‰-45‰; and increasing the salinity to 70‰-75‰ in the postlarval stage until the target shrimp is harvested, wherein the biological filler comprises zeolite, Bacillus natto, planktonic Clostridium and an immobilized salt solution preparation, wherein the immobilized salt solution preparation is obtained by using modified expansive soil as a carrier to adsorb a salt solution.

The utility model discloses a shellfish larva selecting and filtering device which comprises a bottom plate, a fixing frame is fixedly connected to the upper surface of the bottom plate, a rotating frame is rotationally connected to the inner side wall of the fixing frame, a second mounting ring is fixedly connected to the top end of the rotating frame, a first mounting ring is fixedly connected to the inner side wall of the rotating frame, a second selecting and filtering device is mounted on the first mounting ring, and a second selecting and filtering device is mounted on the second selecting and filtering device. A first selector filter is mounted on the second mounting ring. By arranging the first mounting ring, the second mounting ring, the first selection filter and the second selection filter, when larvae are screened, the purpose of selecting and filtering different specifications of shellfish larvae can be achieved, the working time can be shortened, and the selection and filtering efficiency can be improved; by arranging the fixing frame, the rotating frame, the driving swing device, the connecting block and the sliding column, the driving rotating frame swings back and forth in a reciprocating mode, the situation that part of larvae are blocked and cannot be screened along with accumulation of the larvae is avoided, and the screening quality is improved.

The invention provides a preparation method of high-yield high-temperature-resistant homologous triploid argopecten irradians. The preparation method comprises the following steps: screening parent argopecten irradians with high-temperature-resistant characteristics through a molecular marker-assisted breeding technology; after induced spawning of the parent shellfish, fertilized eggs are treated with cytochalasin B, the fertilized eggs are induced to generate the homologous triploid, the cleavage rate, the hatching rate, the metamorphosis rate and the triploid larva rate are comprehensively considered in the process, and efficient induction of the homologous triploid of the hermaphrodite shellfish is achieved. The high-temperature-resistant molecular marker is developed through SNP sites which are identified through whole genome association analysis and are most remarkably related to the high-temperature-resistant performance of the argopecten irradians, and individuals with high-temperature-resistant genotypes are screened out for spawning and fertilization. Compared with common diploid larvae, the triploid larvae obtained through the method have the higher growth and development speed, and the artificial breeding cost can be greatly reduced.

The invention discloses a breeding method for increasing the metamorphosis rate of chlamys farreri, and belongs to the technical field of chlamys farreri breeding. The number and the putting position of substratum in a breeding pond are reasonably controlled, compound fresh bait is applied, the proportion of microalgae in the bait and the concentration of the applied bait are controlled, and EM bacteria are applied, so that the metamorphosis rate of chlamys farreri is increased; by means of the method, the chlamys farreri larva metamorphosis rate is increased, manual operation and seawater consumption in the larva metamorphosis period are reduced, and the method has important significance in improving the seedling raising effect and improving economic benefits.

This invention discloses a seawater treatment system for aquatic organism seedling and larval cultivation based on a natural ecological cycle. The system includes a natural sedimentation tank, a secondary sedimentation algae cultivation tank, a multi-stage dark sedimentation tank, a primary sand filter, a secondary sand filter, and a water storage tank connected to the end of the secondary sand filter. A pump is installed to connect to a water storage tower. A seawater concentration device and an ultrafiltration device are also included between the water storage tower and the aquatic organism seedling tank. The secondary sedimentation algae cultivation tank can be directly connected to a juvenile shellfish rearing tank for direct use in juvenile shellfish cultivation. The sand filter also features a backwashing design for easy recycling. The seawater processed through the primary and secondary sand filters, concentration device, and ultrafiltration device can be used for larval cultivation of aquatic organisms. This water treatment system, through its multi-stage structural design and combined with natural environmental control of light and shading, avoids the drawbacks of chemical and biological purification and the shortcomings of simple physical sand filtration in a natural and ecological way. This ensures a large-scale supply of water for aquatic organism seedling and shellfish cultivation, meeting the diverse needs of large industrial areas.