24 results about "Larvacea" patented technology

The utility model relates to the technical field of water pumps, in particular to a volute type water taking pump capable of preventing shell vegetables from being attached, an LED lamp strip is installed in a closed cavity defined by a square lamp groove in a water filtering box and the inner wall of a box body, and the shell vegetables are driven to grow, breed and move in the area through the phototaxis of the shell vegetables. Therefore, shell vegetables and larvae are prevented from entering the water filtering tank; furthermore, an ultraviolet lamp strip is mounted in a closed cavity formed by the annular lamp groove and the inner wall of the volute body, so that the shell vegetables and the larvae entering the volute body can be killed, the shell vegetables are prevented from being attached to the inner wall of the volute body or an impeller, and the larvae are also prevented from entering various pipelines at the rear end to grow; secondly, the water inlet electromagnetic valve and the water outlet electromagnetic valve can be closed, the buffer box is screwed off, the high-pressure cleaning assembly is started, the interior of the volute body is thoroughly cleaned once, and attached shell vegetables are washed away; and finally, the buffer box can be screwed off regularly, and the shell vegetables or other objects rushing into the buffer box can be poured out.

The application relates to the field of aquaculture, and particularly discloses a method for cultivating oyster larvae, which comprises the following steps: S1, fertilized eggs are developed into D-shaped larvae in seawater at 22 DEG C for 20-24 hours; S2, the D-shaped larvae are cultivated under the condition that the temperature is 22-24 DEG C and the salinity is 29-31 ‰, feed and growth nutrient solution are fed daily, water is changed daily, after the D-shaped larvae appear eye spots, a larval collector is put in, and algal feed and growth promoting solution are fed daily, the larvae are cultivated into 0.8-1 mm juvenile shellfish, and are transferred to sea cultivation; the growth nutrient solution comprises the following components in weight parts: 1-2 parts of a small molecule peptide solution, 0.01-0.03 parts of neomycin sulfate powder, 0.5-1 part of corn starch, 2-4 parts of modified montmorillonite, and 10-15 parts of water. The method for cultivating oyster larvae has the advantages that the survival rate of the larvae is improved, the growth speed is accelerated, and the metamorphosis and attachment rate is improved.

The invention discloses a method for enhancing adhesion ability of coral larvae after enhancement and release. According to the method, coral larvae (such as coral plankton larvae) are exposed to a 40 mM calcium chloride (CaCl2) solution to be treated for 12-24 hours, and then enhancement and release are carried out. The treated larvae show remarkably enhanced adhesion ability after releasing, the accumulated adhesion rate within 3 days reaches 66.33% and is increased by 240% compared with that of a control group in the same period, and the average survival rate reaches 98.14%. Meanwhile, the transverse growth area and the budding number of attached larvae are remarkably increased, and the density of zooxanthellae, the photosynthetic efficiency and the chlorophyll content have no negative effects. The CaCl2 solution is used for successfully inducing the continuous attachment effect of the coral larvae, the method is easy and convenient to operate, low in cost and environmentally friendly, the attachment success rate of enhancement and release of the coral larvae and the subsequent growth performance of the larvae are remarkably increased, and the method is suitable for repairing and protecting a coral reef ecological system.

The invention discloses a method for efficiently producing triploid oysters, and belongs to the technical field of aquaculture breeding. The method comprises the following steps: S1, obtaining sperms and ova of diploid oysters, and carrying out sperm-ova mixed fertilization; s2, performing induction treatment on the fertilized eggs to inhibit release of a second polar body; s3, collecting fertilized eggs subjected to induction treatment, cleaning, and hatching to a larva stage; s4, 24-72 hours after fertilization, screening based on larva individual size differences, and collecting a target larva population; and S5, culturing the larvae screened in the step S4. According to the method provided by the invention, even if the initial induction rate is only about 70%, the larva population with the triploid rate higher than 90% can be stably obtained finally, the hatching rate and the survival rate are high, the method is easy and convenient to operate, and the requirement for induction conditions is low; the method is suitable for triploid preparation of precious oyster varieties in which a large number of fertilized eggs are difficult to obtain under strong induction conditions or oyster strains with important economic characters.

The invention discloses a shellfish larva vitality measuring device, and relates to the field of marine bivalve shellfish larva behavioristics, a measuring device is arranged in a heat preservation device, swimming tracks and swimming speeds of shellfish larvae in different temperature environments are simulated through the heat preservation device, and the measuring device comprises a measuring chamber, a camera and a measuring scale, a measuring ruler is arranged on the inner side wall of the measuring chamber, the camera faces the measuring chamber and is arranged on the opposite side of the measuring ruler, the camera records the motion trail of the shellfish larvae in the measuring chamber in the background of the measuring ruler, and the clearness of the motion trail of the shellfish larvae in the video is increased through irradiation of the light source. The swimming speed of the shellfish larvae is calculated by combining a measuring scale in a video, so that the accuracy of shellfish larva vitality evaluation is improved; meanwhile, the invention further discloses a determination method applying the shellfish larva vitality determination device, and the vitality strength of the shellfish larvae under the actual condition is judged.

The utility model discloses an integrated device for indoor induction of young coral reproduction and larva collection. The integrated device comprises a main body frame, a seawater circulating system and a high-salt titration system, the main body frame adopts a layered structure design, and the space is divided into an upper layer functional area and a lower layer functional area through an isolation frame; the seawater circulating system consists of a circulating water pool, an efficient water pump, an upper water pipe, an induction cylinder, a collecting tank and a lower water pipe; the high-salt titration system is connected with the induction cylinder and is used for accurately regulating and controlling the salinity of the water body. According to the utility model, 1) a modular design is adopted, and functions of temporary culture of coral parent, induction of larva discharge, collection of larvae and the like are integrated into a whole; dynamic adjustment of seawater salinity is achieved through a high-precision salinity titration system, environmental parameters in the induction cylinder can be accurately controlled through cooperation with a temperature control device so as to induce coral larva discharge, an overflow structure is adopted, the water level in the induction cylinder rises, larvae can be collected into a collecting barrel through an overflow opening, and efficient collection of the larvae is achieved.

The invention relates to the technical field of water environment monitoring, and discloses a water environment monitoring device which comprises a floating body, a water quality sensor and a lifting mechanism. The lifting mechanism is arranged on the floating body and used for driving the water quality sensor to descend to the preset depth of seawater or ascend; after reaching the preset depth, the water quality sensor is in contact with seawater for water quality monitoring; the cleaning assembly comprises an anode bar and a cathode tube which are located at the bottom end of the water quality sensor and concentrically arranged inside and outside and used for cleaning the outer surface of the water quality sensor. The hypochlorous acid diffuses to the surface of the water quality sensor, and because the hypochlorous acid is extremely thin and has strong oxidizing property, the hypochlorous acid can destroy cell walls of planktons and larvae, interfere neuromuscular functions of the planktons and the larvae and effectively inhibit key physiological processes of the planktons and the larvae in metabolism and early growth periods; therefore, larvae and spores of organisms such as algae and shellfish are inhibited from settling and growing on the surface of the water quality sensor.

The present application belongs to the technical field of aquaculture, and particularly relates to a method for improving survival rate of Dendostrea folium shell top larvae by dry exposure treatment. In the present application, the Dendostrea folium larvae in the late shell top stage are subjected to dry exposure stress treatment, and then rehydrated, fed and cultivated. Compared with the control group of oyster larvae without dry exposure stress treatment, the survival rate of the larvae cultivated by the shell top larvae subjected to dry exposure stress treatment is significantly improved under high temperature, low temperature and low oxygen stress. The present application can significantly improve the environmental stress resistance of Dendostrea folium larvae, and has a good application prospect in shellfish seed production.

The application relates to a breeding method of robust oysters, belonging to the field of animal genetics and breeding, which comprises population selection, energy recovery of larvae and selection of robust oysters; the selection of the robust oysters is carried out by selecting larvae with strong phototaxis and strong swimming ability by using the phototaxis of the oyster larvae. The robust oysters with the advantages of fast growth speed, large individual, fast attachment rate, disease resistance and stress resistance can be bred by selecting the individuals with strong phototaxis.

The present application relates to a kind of bait feeding mode suitable for northern oyster larva breeding work, at the D type larva period of northern oyster, feed mixed bait of chrysomonad and chlorella.The present application significantly improves the growth rate and survival rate of long oyster larvae by feeding mixed bait of chrysomonad and chlorella, and shortens the breeding cycle.

The application discloses a kind of high-efficiency promoting coral larvae attachment metamorphosis and the growth of complex microbial inoculant and application thereof.The bacterium agent comprises Phytobacter sp.SCSIO43973 and Salipiger sp.SCSIO43974 two strains, and preservation numbers are respectively: GDMCC NO:65583 and 65650.The two strains are isolated from healthy antler cup-shaped coral.It is shown that the addition of these strains can significantly improve the attachment rate, metamorphosis rate of planulae, and the survival rate and budding rate of coral larvae.These characteristics show that the bacterium agent has the potential to improve the attachment metamorphosis rate of coral larvae and promote the growth in the field coral reef ecosystem, and has important significance for increasing the amount of larvae supplement, promoting the recovery and sustainable development of coral reef ecosystem.The above, the microbial inoculant of the application promotes the attachment metamorphosis of coral larvae and the growth of larvae, and provides a new technical means for the recovery of coral reef ecosystem.

The present application relates to a breeding method of hybrid seed of Babylonia formosae and Babylonia areolata. The breeding method comprises the following steps: breeding Babylonia formosae parent snails and Babylonia areolata parent snails in the dark, feeding 3-5% of the body weight of Babylonia formosae with vegetables, and feeding 10-15% of the body weight of Babylonia formosae with mixed biological bait, continuously feeding until the gonad matures, and feeding at least once a day; obtaining egg sac clusters by hybridizing Babylonia formosae parent snails and Babylonia areolata parent snails; hatching the egg sac clusters to form planktonic larvae, cultivating the planktonic larvae for 15-20 days, and metamorphosing the planktonic larvae into juvenile snails to obtain hybrid seed. The hybrid seed obtained by the breeding method has excellent metamorphosis rate, fertilization rate and hatching rate, and the average body weight, average shell length and survival rate are also significantly improved.

The invention discloses an efficient preparation method of marine pearl oyster larvae. Through the technical links of gamete maturity evaluation, gonad mucus removal, ovum maturation promotion and activation, sperm motility excitation, excessive sperm fertilization and the like, the hatching rate of 90% or above is obtained, and therefore the purpose of efficient larva preparation is achieved. The method is different from the traditional method of dissecting mixed sperms and eggs for filtering, placing the sperms and eggs in a hatching container for settling for 1 hour, then pouring out supernate and directly scattering the sperms and eggs settled to the bottom into a pool for hatching, the solidification cognition of traditional marine pearl oyster breeding is subverted, the defects of low hatching rate, poor stability and the like of the traditional method are overcome, a large number of high-quality larvae are obtained, and the breeding efficiency is improved. And a technical guarantee is provided for high-efficiency production of offspring seeds, heterosexual pairing breeding and creation of excellent strains in the future. The method has the advantages of simplicity and convenience in operation, high repetition rate, high practicability and the like.

The invention belongs to the technical field of aquaculture breeding, and discloses a cultivation method of a new oligotrophic oyster strain. The method comprises the following steps: constructing an oyster larva mixed population with the same genetic background; when the larvae develop to a shell top larva stage, starvation stress treatment is started, the starvation stress treatment is accurate limited bait based on feeding rate feedback, and feeding is performed by halving the feeding rate; after the hunger stress treatment is finished, screening the larvae by using a screen, and dividing the individuals which are retained in the screen and have the shell length of more than or equal to 180 microns into oligotrophic resistance groups; and continuing to cultivate the screened oligotrophic group, so as to obtain the oligotrophic oyster strain. According to the method, the core breeding period is revolutionarily shortened to the day level from the month level, and the oligotrophic oyster strain bred through the method has the advantages that the higher bait conversion rate is achieved in the larva stage, and the genetic advantage can be stably expressed in the whole growth period.

The application discloses a preparation method and application of efficiently obtaining solar long-spined sea star larvae and juveniles / larvae. The method comprises the following steps: distinguishing the development period and the male and female genders of gonads; injecting oxytocin, separately collecting eggs and sperms, adding active sperms in batches after the eggs are broken, and the quantity ratio of the sperms and the eggs is 50-100:1; controlling the temperature, salinity and pH during the hatching of the larvae, culturing under full or half light shielding, and stirring when the fertilized eggs gather; feeding the hatched larvae with small new moon-shaped algae, changing water and removing dead and abnormal individuals; increasing the amount of feed when the larvae develop into brachiolaria, adding an attachment base when 70% of the planktonic larvae develop into late-stage brachiolaria with a size of 1000mu m*600mu m, transferring the juveniles to living stones with benthic coral algae after metamorphosis, and culturing until the long-spined sea star larvae are harvested. The application significantly improves the survival rate and metamorphosis rate of the planktonic larvae, and efficiently obtains a batch of larvae and juveniles / larvae in a short time.

The present invention proposes a biofilm-based high-salt culture method for whiteleg shrimp, which is characterized by comprising the following steps: adding a biological filler to a culture pond once when the whiteleg shrimp is in the flea-like larval stage to increase the salinity to 20‰-25‰; adding the biological filler to the culture pond a second time in the mysid stage to increase the salinity to 40‰-45‰; and increasing the salinity to 70‰-75‰ in the postlarval stage until the target shrimp is harvested, wherein the biological filler comprises zeolite, Bacillus natto, planktonic Clostridium and an immobilized salt solution preparation, wherein the immobilized salt solution preparation is obtained by using modified expansive soil as a carrier to adsorb a salt solution.

The invention discloses a breeding method for increasing the metamorphosis rate of chlamys farreri, and belongs to the technical field of chlamys farreri breeding. The number and the putting position of substratum in a breeding pond are reasonably controlled, compound fresh bait is applied, the proportion of microalgae in the bait and the concentration of the applied bait are controlled, and EM bacteria are applied, so that the metamorphosis rate of chlamys farreri is increased; by means of the method, the chlamys farreri larva metamorphosis rate is increased, manual operation and seawater consumption in the larva metamorphosis period are reduced, and the method has important significance in improving the seedling raising effect and improving economic benefits.

This invention discloses a seawater treatment system for aquatic organism seedling and larval cultivation based on a natural ecological cycle. The system includes a natural sedimentation tank, a secondary sedimentation algae cultivation tank, a multi-stage dark sedimentation tank, a primary sand filter, a secondary sand filter, and a water storage tank connected to the end of the secondary sand filter. A pump is installed to connect to a water storage tower. A seawater concentration device and an ultrafiltration device are also included between the water storage tower and the aquatic organism seedling tank. The secondary sedimentation algae cultivation tank can be directly connected to a juvenile shellfish rearing tank for direct use in juvenile shellfish cultivation. The sand filter also features a backwashing design for easy recycling. The seawater processed through the primary and secondary sand filters, concentration device, and ultrafiltration device can be used for larval cultivation of aquatic organisms. This water treatment system, through its multi-stage structural design and combined with natural environmental control of light and shading, avoids the drawbacks of chemical and biological purification and the shortcomings of simple physical sand filtration in a natural and ecological way. This ensures a large-scale supply of water for aquatic organism seedling and shellfish cultivation, meeting the diverse needs of large industrial areas.

This invention discloses a method for inducing coral planktonic larvae to improve their attachment ability in the field of planktonic larvae induction technology, comprising the following steps: Step 1, cutting shell-like coral algae to a certain particle size to obtain shell-like coral algae particles, and then filling the shell-like coral algae particles into porous shell-like coral algae to obtain an induction culture substrate; Step 2, placing planktonic larvae on the induction culture substrate, then placing them in an induction culture container, and immersing them in seawater at a temperature of 27°C for 2-7 days; Step 3, keeping the seawater warm through the induction culture container, and after 2-7 days, raising the temperature of the seawater through the induction culture container to continue the induction culture. This invention improves the temperature and induction structure under the condition of utilizing existing induction substrates, thereby enhancing the attachment ability of planktonic larvae.

This invention relates to the field of marine organism rearing technology, specifically providing a method for the low-temperature breeding of moon jellyfish in artificial rearing. The method includes seawater preparation, collection of planktonic larvae, cultivation of polyps, and cultivation of adult jellyfish. The polyps are cultivated at a low temperature of 12-15℃ and fed with rotifers and nutrients. During the cultivation of adult jellyfish, *Lactobacillus plantarum* is used to assist in the cultivation. This method, through low-temperature cultivation and the addition of nutrients, improves the attachment rate of planktonic larvae, the survival rate of polyps and discoid bodies, and increases the size of adult jellyfish, significantly improving the breeding efficiency and quality of moon jellyfish.

The invention belongs to the technical field of gene editing, and particularly relates to a gene editing method for obtaining a bivalve adult edit. The method specifically comprises the following steps: by taking a target gene which has a visual phenotype in appearance and is not lethal in early development as a marker, designing a targeting site sgRNAs based on the marker, and constructing a Cas9 / sgRNAs edit body by virtue of an electrotransfection system, so as to realize gene editing of bivalve mollusks and obtain an adult edit body. According to the method, the technical bottlenecks that the existing bivalve mollusk gene editing is only limited to the larva development stage and the edit adult screening is difficult are broken through, and a new technical system is provided for the creation of bivalve mollusk edit germplasm materials. In addition to oysters, the technical system is also suitable for other bivalve shellfishes with small fertilized eggs, difficulty in micro-injection and high larva culture death rate.

The invention relates to the technical field of ciona ciona breeding, and particularly discloses a ciona ciona full-artificial breeding method which is carried out according to the growth stage and comprises the following steps: firstly collecting mature ciona ciona gametes for artificial insemination, and culturing fertilized eggs by using filtered seawater containing antibiotics in a specific ratio; then floating / swimming larvae are screened, cleaned, subjected to multi-round state judgment and antibiotic replacement and transferred to a breeding tank through suspension culture, water is replaced every day, and dead individuals are observed and removed; subsequently, according to different stages of larvae, adults, sexually mature adults and aged adults, the culture time, the feeding frequency and the bait dosage are adjusted; in the sexual maturity stage, self-fertilization embryos need to be collected in a specific photoperiod. By means of the method, ciona glabra can be efficiently cultivated, the survival rate of all stages is guaranteed, and technical support is provided for large-scale cultivation of ciona glabra.

The application provides a preparation method of high-yield high-temperature-resistant homologous triploid Argopecten irradias, and the Argopecten irradias parent shellfish with high-temperature-resistant characteristics is screened through a molecular marker assisted breeding technology; after the parent shellfish is induced to spawn, the fertilized eggs are treated by using cytochalasin B to induce the generation of homologous triploidy, and the cleavage rate, the hatching rate, the metamorphosis rate and the triploid larva rate are comprehensively considered, so that the high-efficiency induction of the homologous triploid of the hermaphrodite shellfish is realized. The SNP site most significantly related to the high-temperature-resistant performance of the Argopecten irradias is identified through whole genome association analysis, a high-temperature-resistant molecular marker is developed, and the individual with the high-temperature-resistant genotype is screened for spawning and fertilization. The triploid larva obtained through the method has a faster growth and development speed than the common diploid larva, and the artificial breeding cost can be greatly reduced.

The application provides a feed additive suitable for beach shell plankton larvae and a preparation method and application thereof, and the feed additive is prepared from core material and wall material in a mass ratio of (1-2):(2-1); raw materials of the core material include 2-3 parts of leucine, 2-3 parts of isoleucine, 2-3 parts of valine, 2-3 parts of taurine, 0.5-1 part of high-temperature sterilized sea mud and 85-95 parts of water; and raw materials of the wall material include 3-7 parts of beta-cyclodextrin, 3-7 parts of methyl cellulose and 85-95 parts of water. The preparation method comprises the following steps: S1, preparing a core material solution and a wall material solution; S2, mixing and stirring to obtain a mixed solution; and S3, spray drying the mixed solution to prepare microcapsules and storing, and the preparation is completed. The feed additive prepared by the application can effectively improve the survival state of the plankton larvae, solves the problem that direct feeding of amino acids has an influence on water quality, and has the advantages of simple production process, stable procedure, easy control, high success rate, and suitability for enterprises and breeders.