Canine parvovirus resistant refined antibody and preparation method thereof

A canine parvovirus and antibody technology, which is applied in the preparation methods of peptides, antiviral agents, antiviral immunoglobulins, etc., can solve the problems of low cure rate of canine parvovirus, maintain integrity and biological activity, improve protein Purity, the effect of increasing immunity and resistance

Inactive Publication Date: 2017-10-31
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of treatment, there is no effective prevention and treatment of canine parvovirus drugs and methods, so the cure rate of canine parvovirus is low

Method used

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  • Canine parvovirus resistant refined antibody and preparation method thereof
  • Canine parvovirus resistant refined antibody and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Preparation of refined antibody against canine parvovirus

[0057] 1. Test method

[0058] 1.1 Preparation of hyperimmune serum against canine parvovirus

[0059] Two strains of inactivated vaccines, CPV-S5 (New CPV-2a) and CPV-1401 (New CPV-2b), were prepared according to conventional inactivated vaccine preparation methods. Select a certain number of healthy puppies with negative CPV HI titer, inoculate two inactivated vaccines, CPV-S5 and CPV-1401, subcutaneously on the back, and carry out booster immunization when the HI titer drops below 1:80 (about 4 weeks) .

[0060] 10 to 14 days after the completion of the second vaccination, the antibody titer was measured by the hemagglutination inhibition test (HI). Dogs with an antibody titer of not less than 1:2048 were selected for aseptic blood collection from the carotid artery, mixed and stored at low temperature.

[0061] 1.2 Saturated ammonium sulfate (AS) step-by-step salting-out

[0062] (1) Thaw the ...

Embodiment 2

[0086] Example 2 Safety Detection of Anti-Canine Parvovirus Antibody Refined Antibody

[0087] 1. Experimental method

[0088] 5-month-old Beagle dogs were injected subcutaneously and intramuscularly at doses of 1.0, 2.0, and 4.0 mL per kilogram of body weight, respectively. They were observed continuously for 48 hours and kept for 3 months. dietary status.

[0089] 2. Experimental results

[0090] Beagle dogs injected with different doses of purified anti-canine parvovirus antibodies were observed continuously for 48 hours. There was no abnormal phenomenon such as swelling and allergies at the injection site, and their body temperature and mental and dietary conditions were normal. Continue to feed for 3 months, during which there is no abnormality, indicating that the anti-anti-canine parvovirus refined antibody has no side effects and is suitable for clinical promotion.

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Abstract

The invention discloses a canine parvovirus resistant refined antibody and a preparation method thereof. The preparation method comprises the following steps: S1, preparing canine parvovirus inactivated vaccines by taking canine parvovirus CPV-S5 and CPV-1401 separated and identified in Guangdong regions as basic strains; S2, preparing canine parvovirus hyper-immune serum from vaccine immunity healthy dogs; S3, separating canine parvovirus resistant immune globulin in the serum prepared in the S2 through saturated ammonium sulfate stepped salting-out; S4, purifying, namely desalting the canine parvovirus resistant immune globulin extracted in the step S3 through dextrangel; S5, performing ultrafiltration concentration on the canine parvovirus resistant immune globulin subjected to desalting treatment in the step S4, filtering to remove bacteria, and performing split charging, thereby obtaining the product. According to the canine parvovirus resistant refined antibody prepared by the invention, the protein content reaches 134mg/ml, and the hemagglutination inhibition titer is not lower than 1:10240; and the canine parvovirus resistant refined antibody can be applied to treatment and emergency prevention when dogs of different varieties are in contact infection with the canine parvovirus, is capable of increasing the organism immunity and resistance of ill dogs, does not cause any adverse reaction, does not have any clinical side effect, has high safety and has excellent popularization and application prospects.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals. More specifically, it relates to a refined anti-canine parvovirus antibody and a preparation method thereof. Background technique [0002] Canine parvovirus disease, also known as canine parvovirus enteritis, is a highly contagious infectious disease caused by canine parbovirus (CPV), mainly causing acute hemorrhagic enteritis in dogs or acute myocarditis in newborn dogs. The main clinical symptoms are elevated body temperature, severe vomiting, hemorrhagic enteritis and significant decrease in white blood cells. The disease occurs all year round, especially in spring and autumn. Dogs of different types and ages can be infected with the disease. Among them, puppies are the most harmful, with the highest infection rate and mortality rate, which seriously endangers the dog industry in my country. At present, vaccine immunization is used to prevent the disease to a certain extent, but d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/08C07K16/06C07K1/36C07K1/34C07K1/30C07K1/16A61K39/42A61P31/20A61P1/00A61P37/04
CPCA61K39/00C07K16/065C07K16/081
Inventor 郭霄峰畅翊然刘运忠黄永亮朱盛和叶苹苹罗均施赫赫牛学锋
Owner SOUTH CHINA AGRI UNIV
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