Canine distemper and parvovirus disease bivalent inactivated vaccine for dogs and preparation method of canine distemper and parvovirus disease bivalent inactivated vaccine

A dual inactivated vaccine and canine distemper virus technology, applied in veterinary vaccines, biochemical equipment and methods, vaccines, etc., can solve the high infection rate and fatality rate of parvovirus in dog breeding and economic animal breeding losses, Immunization effects of live vaccines are greatly reduced and other issues

Active Publication Date: 2021-02-26
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The high infection rate and fatality rate of canine parvovirus have caused huge losses to the dog industry and the breeding of commercial animals
[0004] At present, most of the dual vaccines for canine distemper and canine parvovirus are attenuated live vaccines. On the one hand, live vaccines have the risk of spreading the virus; It may be affected by it. In practical applications, it is difficult to grasp the timing of immunization with live vaccines for puppies before and after weaning. When the maternal antibody is at a high level, the immune effect of the live vaccine will be greatly reduced. At a low level of immunity, potential infection may have occurred before immunization; compared with live vaccines, the immunity of inactivated vaccines is less affected by maternal antibodies. Therefore, it is safe, effective, long-lasting, and easy to store and transport. Canine distemper and parvovirus dual-inactivated vaccine for dogs is an effective method to control canine distemper and parvovirus disease in puppies. It is an urgent need of the industry and the market to realize "one shot to prevent two diseases" and has a broad market prospect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation of embodiment 1 canine distemper, parvovirus disease dual combination inactivated vaccine

[0033] (1) Inoculate the CDV-LG strain of canine distemper virus on Vero cells of African green monkey kidney cells to carry out virus amplification and culture, and harvest the virus liquid when the cytopathic changes reach 80%;

[0034] (2) Inoculate the canine parvovirus CPV-W strain on the cat kidney cell F81 to carry out virus amplification and culture, and harvest the virus liquid when the cytopathic changes reach 80%;

[0035] Specifically:

[0036] Preparation of poisonous seeds for production

[0037] Virus breeding

[0038] For the CDV-LG strain of canine distemper virus, dilute the virus seeds with MEM medium, absorb and inoculate Vero cells according to the MOI of 0.1-0.3 inoculum, cultivate at 37°C for 24 hours, change the maintenance medium, and cultivate at 33°C. % or more, freeze-thaw to collect the poison, and store below -20°C.

[0039] For t...

Embodiment 2

[0080] Example 2 Safety Test of Canine Distemper and Parvovirus Dual Inactivated Vaccine for Dogs

[0081] Get 3 batches of qualified canine distemper and parvovirus dual inactivated vaccines for dogs (prepared by the method of embodiment 1), inoculate healthy susceptible dogs, and establish single-dose group, single-dose repeated group and overdose (10 times) In the immunization group, dogs not vaccinated were set as controls. After vaccination, observe continuously for 14 days to observe whether there is any abnormality in the dog's body temperature, appetite, mental state, and feces. No clinical symptoms were shown, and no lesions were observed in the main organs of the inoculated Beagle dogs, and there was no obvious inflammatory reaction at the inoculation site. It shows that the canine distemper and parvovirus dual inactivated vaccine is safe for dogs.

Embodiment 3

[0082] Example 3 Efficacy Test of Canine Distemper and Parvovirus Dual Inactivated Vaccine for Dogs

[0083] 1. Method

[0084] (1) Get canine distemper virus neutralizing antibody, canine parvovirus hemagglutination inhibitory antibody negative canine distemper, parvovirus disease double inactivated vaccine 3 batches (prepared by the method of embodiment 1), inoculate antibody negative dog, One head per animal, and a control group inoculated with cell culture medium, blood was collected every 3 months after immunization, and serum was separated for detection of neutralizing antibodies and hemagglutination inhibitory antibodies.

[0085] (2) 3 months, 6 months and 9 months after the dog was vaccinated, the canine distemper and parvovirus virulent viruses were used for the test respectively for challenge inoculation, and the challenge dose was 100 LD for each virulent virus 50 / Only. Observe the clinical symptoms such as body temperature, appetite, feces, and mental state of ...

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PUM

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Abstract

The invention discloses a canine distemper and parvovirus disease bivalent inactivated vaccine for dogs and a preparation method of the canine distemper and parvovirus disease bivalent inactivated vaccine, and relates to a vaccine preparation method. The effective components of the vaccine comprise an inactivated canine distemper virus CDV-LG strain and an inactivated canine parvovirus CPV-W strain, and safety tests show that no adverse reaction occurs when the bivalent inactivated vaccine is used for inoculating dogs; and efficacy tests show that within 6 months after single-dose vaccination,the dogs can be prevented from being attacked by virulent viruses for canine distemper and parvovirus disease examination, and the dogs can be protected. The bivalent inactivated vaccine adopts a canine distemper virus and canine parvovirus inactivation process, can be used for preventing canine distemper and canine parvovirus disease at the same time, realizes prevention of two diseases by one injection, and has wide application prospects.

Description

technical field [0001] The invention relates to a vaccine preparation method, in particular to a dual inactivated vaccine for canine distemper and parvovirus disease and a preparation method thereof. Background technique [0002] Canine distemper is an acute, febrile, highly contagious infectious disease caused by canine distemper virus belonging to the genus Morbillivirus in the Paramyxoviridae family. Other pneumonia, gastroenteritis, and occasional neurological symptoms are prone to secondary mixed infection and secondary infection of other bacteria and viruses, and the mortality rate can be as high as 80%. Virus-carrying animals are the most dangerous source of infection. Viruses are excreted through eye and nasal secretions, saliva, urine, and feces, and are mainly transmitted through the digestive tract and respiratory tract. In recent years, with my country's military dogs, police dogs, experimental dogs and pets With the substantial increase in the amount of dog bree...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61K39/175A61K39/23A61P31/20A61P31/14
CPCA61K39/12A61P31/14A61P31/20A61K2039/552C12N2760/18434A61K2039/70C12N2750/14334A61K2039/5252Y02A50/30
Inventor 罗国良冯二凯程悦宁王振军易立
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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