3results about How to "Retention" patented technology

The application discloses a kind of for sweat glucose concentration monitoring method, it is related to sweat monitoring field, comprising the following steps: step 1, enzyme-fixed chain conjugate synthesis: glucose dehydrogenase is reacted with bilirubin oxidase respectively with bifunctional crosslinking agent and is purified, obtain enzyme-crosslinking agent intermediate;It is clicked with azide modified DNA fixed chain chemistry reaction again, and after purification, obtain GDH-fixed chain and BOx-fixed chain conjugate;Step 2, preparation of circular DNA template: after annealing hybridization of phosphorylated template chain and primer chain, it is connected into ring by T4 DNA ligase;Step 3, rolling circle amplification self-assembly package enzyme DNA hydrogel;The present application uses super-flexible DNA hydrogel as flexible electrode material, realizes the efficient fixation and continuous stable catalysis of GDH and BOx, and constructs flexible sweat-based EBFC self-powered sensor as flexible bioelectrode, to realize continuous non-invasive blood glucose monitoring.

The present application belongs to the field of biological experimental technology, and specifically relates to a brain stereotaxic combined with continuous gas anesthesia drug delivery system and a brain stereotaxic anesthesia depth quantitative adjustment method. A brain stereotaxic combined with continuous gas anesthesia drug delivery system, characterized in that it includes a first ear bar, a second ear bar, an anesthesia headgear and a gas delivery device; the anesthesia headgear is used to deliver anesthetic gas and / or oxygen to the experimental model and assist the first ear bar and the second ear bar to complete the fixation of the experimental model. The present application improves the structure and function of the original brain stereotaxic instrument, making it an animal brain stereotaxic instrument that can fix rats and give continuous gas anesthesia, avoiding experimental errors and accidental deaths caused by too shallow or too deep anesthesia depth of animals during the experiment, and has technical effects such as more stable model state, higher experimental accuracy, and experimental results closer to the actual situation.

The utility model relates to an ear drug delivery device for the otolaryngology department. The ear drug delivery device comprises a handheld assembly and a fixing assembly which are used in cooperation. For the handheld assembly, the liquid guide pipe penetrates through the handheld part from bottom to top, and the pipe diameter of the rubber head medicine dripping pipe is matched with the pipe diameter of the liquid guide pipe. The top end of the first connecting rod is fixed to the bottom face of the handheld part, and the bottom end of the first connecting rod is fixed to the outer side of the top of the limiting inner sleeve. The limiting inner sleeve piece is of a hollow lantern ring structure, and the liquid guide pipe penetrates through the limiting inner sleeve piece from top to bottom. The top end of the second connecting rod is fixed to the inner side of the bottom of the limiting inner sleeve, and wiping cotton is fixed to the bottom end of the second connecting rod. For the fixing assembly, the limiting outer sleeve piece is fixed to the sleeve lug ring; the limiting outer sleeve piece is of a hollow lantern ring structure, and a circular clamping groove matched with the bottom end of the limiting inner sleeve piece is formed in the top of the limiting outer sleeve piece. On the basis of the device, during ear administration, accidental injury to the interior of an ear hole can be avoided while various liquid medicines are prevented from volatilizing medicine properties.