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Combined live vaccine against porcine reproductive and respiratory syndrome and swine fever, and application thereof

A technology for respiratory syndrome and swine fever vaccine, which is applied in the field of preparation of porcine reproductive and respiratory syndrome and swine fever combined live vaccine, can solve the problem of time-consuming, increased cost, multiple injections, multiple doses, and difficulty in multiple immunization procedures, etc. question

Active Publication Date: 2014-05-14
华威特(江苏)生物制药有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, swine fever is also one of the diseases listed as compulsory immunization in my country. Therefore, in the process of epidemic prevention, it is difficult to rationally arrange multiple injections, multiple doses, and multiple immunization procedures, which are cumbersome, time-consuming, laborious, and increase costs. , which directly affects the immune effect

Method used

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  • Combined live vaccine against porcine reproductive and respiratory syndrome and swine fever, and application thereof
  • Combined live vaccine against porcine reproductive and respiratory syndrome and swine fever, and application thereof
  • Combined live vaccine against porcine reproductive and respiratory syndrome and swine fever, and application thereof

Examples

Experimental program
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Embodiment 1

[0050] (1) Passage and cultivation of cells for seedling preparation: the Marc-145 cell line used for the production of highly pathogenic porcine reproductive and respiratory syndrome TJM strain virus was digested and passed on by trypsin-EDTA cell dispersion, and passed on one to three; The BT cell line used for the production of the attenuated strain of hog fever lathe virus was digested and passaged with trypsin-EDTA cell dispersion, and the passage was carried out at a ratio of 1 to 5; the cell growth solution was added to continue culturing at 37°C, and when a good monolayer was formed, it was used for further passage. passaging or inoculation of viruses;

[0051](2) Propagation of cytotoxic species: the highly pathogenic porcine reproductive and respiratory syndrome TJM strain virus species is inoculated according to the inoculation amount of MOI0.01-0.5, cultivated for 3-5 days after inoculation, when the cytopathic effect reaches 70% Harvest the virus liquid; make a 0....

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Abstract

The invention provides a preparation method for a combined live vaccine used for preventing high pathogenic porcine reproductive and respiratory syndrome and swine fever and a product thereof. According to the invention, no immunosuppression occurs between an attenuated vaccine strain of high pathogenic porcine reproductive and respiratory syndrome and an attenuated vaccine strain of swine fever; the combined live vaccine prepared from the two attenuated vaccine strains is identical with each single vaccine in the aspects of security, immunogenicity, immunity duration and immuno-protective effects and has remarkable immuno-protective effects on preventing porcine reproductive and respiratory syndrome and swine fever. When the product provided in the invention is used to immunize and inoculate animals, two diseases can be prevented simultaneously with only one injection, which enables work load of immunization and inoculation to be mitigated, immunization frequency to be reduced, stress on swinery to be decreased, and immunological paralysis and immunological failure caused by frequent immunization to be avoided. The product of a vaccine preparation in the invention further has the advantages of a long storage life and storage stability.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and more specifically relates to a method for preparing porcine reproductive and respiratory syndrome and swine fever dual live vaccine and its product. Background technique [0002] At present, porcine reproductive and respiratory syndrome (PRRS) and swine fever (CSF) are two important infectious diseases that seriously endanger the pig industry in my country. Since the outbreak of highly pathogenic porcine reproductive and respiratory syndrome (also known as Highly pathogenic blue-ear disease), has caused huge economic losses to my country's pig industry, and is listed as one of the epidemic diseases for compulsory immunization by the Ministry of Agriculture. At the same time, swine fever is also one of the diseases listed as compulsory immunization in my country. Therefore, in the process of epidemic prevention, it is difficult to rationally arrange multiple injections, m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61P31/14A61K39/12A61K39/187
Inventor 武华夏铭崎和彦良
Owner 华威特(江苏)生物制药有限公司
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