Construction method for immune model of cryptocaryon irritans locally-infected epinephelus coioides skin

A technique for stimulating Cryptocaryonia and grouper, applied in the construction of immune models and in the field of fish mucosal immunity, can solve problems such as differences in immune responses that cannot be ruled out, and achieve the goal of reducing individual differences or tissue differences in fish Effect

Active Publication Date: 2018-09-28
SOUTH CHINA AGRI UNIV
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Problems solved by technology

However, this method cannot rule out differences in the immune response of the same fish under normal conditions in the tail and skin other than the tail

Method used

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  • Construction method for immune model of cryptocaryon irritans locally-infected epinephelus coioides skin

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specific Embodiment approach

[0026] A method for constructing an immune model for stimulating the local infection of Cryptocaryon spp. to the skin of grouper, comprising the following steps:

[0027] 1. Stimulate the collection and concentration calculation of live Cryptocaryon larvae

[0028] Cryptocaryon stimuli were subcultured and collected using a special subculture system for Cryptocaryon stimuli, and the subculture host was Pompano ovata. Collect well-developed Cryptocaryon irritant cysts, place them in a sterilized clean beaker and incubate them with high-pressure sterilized natural seawater with appropriate amount of penicillin and streptomycin added; collect irritant cryptocysts within 2 hours of hatching. Take 50 μL of nuclei larva fluid after mixing thoroughly, add 10 μL of formaldehyde to kill the larvae, count under a microscope, and calculate the concentration of larvae in the fluid.

[0029] 2. Grouping and domestication of grouper

[0030] The fish used in the experiment is about 20 g g...

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Abstract

The invention adopts a method of cryptocaryon irritans locally-infected epinephelus coioides one-sided skin for immunity for the first time, adopts an optimized infection concentration and infection duration time to construct an immune model of the cryptocaryon irritans locally-infected epinephelus coioides one-sided skin for the first time, thoroughly eliminates an adverse effect brought by ''congenital differences'' in the process of local skin mucosal immune response in fish, and provides an ideal immune model for researching a local mucosal immune response law. The construction method forthe immune model mainly comprises the following five steps: (1) collecting live larvae of the cryptocaryon irritans and calculating a concentration; (2) grouping the epinephelus coioides and performing domestication; and (3) performing local skin immunization; (4) measuring infection intensity; and (5) performing treatment on locally-infected fish bodies.

Description

technical field [0001] The invention belongs to the field of fish immunity, in particular to the direction of fish mucosal immunity, and specifically relates to a method for constructing an immune model for inducing local mucosal immunity in the skin tissue of a grouper. Background technique [0002] Cryptocaryon irritans (Cryptocaryon irritans) is a protociliate that can infect almost all marine bony fishes and cause severe fatal diseases - Cryptocaryon irritans or white spot disease (Colorni A and BurgessP., 1997) . The life cycle of Cryptocaryon stimuli includes four stages: trophozoite, cyst precursor, cyst and larvae, among which only the trophozoite is an obligate parasitic life and feeds on host body fluids, tissue fragments and intact cells. In recent years, due to the overloaded development of marine aquaculture in my country and the deterioration of the aquaculture environment, Cryptocaryoniasis has become a large-scale, frequent outbreak of severe infectious dise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A01K61/13
CPCA01K61/10A01K61/13Y02A40/81
Inventor 李言伟但学明莫泽权
Owner SOUTH CHINA AGRI UNIV
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