The application discloses a wheat
Tilletia indica-specific biomarker combination, a
screening method and application thereof, and the biomarker combination comprises 20-hydroxyarachidonic acid, 1-octadecyl
lysophosphatidic acid,
linolenic acid, 13-hydroxydocosahexaenoic acid, 4-methoxy
estrone,
hypoxanthine, 3-hydroxymyristic acid, beta-
ecdysterone, 17-phenyltrinorprostaglandin F2 alpha cyclopropylmethylamide, 11,12-dihydroxyeicosatrienoic acid and oxidized
octadecadienoic acid. When the biomarker combination is used for identifying
Tilletia indica, the time-consuming long and the accuracy easily interfered by the
spore development stage limitations of traditional morphological identification are overcome; in view of the
molecular level identification difficulty caused by the high
genome homology of
Tilletia indica and Tilletia controversa, the application digs the feature difference at the level of
metabolomics, and constructs a specific chemical
fingerprint, so that the technical
bottleneck that the
close relatives are difficult to accurately distinguish is effectively solved.