The invention discloses targeted interference equipment and an interference method of wireless local area network. The interference equipment of the wireless local area network comprises a radio frequency receiving module, a frequency scanning module, a physical layer receiving module, a multi-access computer layer (MAC) analyzing and comparison module, an interference data generating module, a radio frequency transmitting module, a power amplifier and an antenna, wherein on one hand, the radio frequency module is connected with the antenna, and on the other hand, the frequency scanning module and the physical layer receiving module are respectively connected with the radio frequency module, the physical layer receiving module is connected with the MAC layer analyzing and comparison module, and the interference data generating module, the radio frequency transmitting module and the power amplifier are respectively connected with the MAC layer analyzing and comparison module. According to the targeted interference equipment and the interference method of wireless local area network, shield interference can be merely conducted by targeting appointed sites without influencing normal communication of other sites in network, and interference to all sites can be achieved. The targeted interference equipment and the interference method of wireless local area network is good in interference effect, wide in range of action and low in energy consumption.