Production method for cryptocaryon irritant prevention trachinotus ovatus seedling
A technology to stimulate Cryptocaryonia and pomfret, which is applied in the production of disease-resistant fry and anti-stimulatory Cryptocaryon fry, can solve the problem that there is no detailed study on the impact of seawater fish's immune protection, and the effective immune protection period is only limited. It can reach 3 months and other problems, and achieve the effect of long effective immune protection period, simple and easy operation, and reduced mortality
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[0026] 1. Stimulation of live Cryptocaryon larvae collection and concentration calculation
[0027] Use a special Cryptocaryon stimuli subculture system to subculture and collect Cryptocaryon stimuli. The subculture host is Pomfret ovata. Collect well-developed Cryptocaryon stimuli cysts and place them in a sterilized clean beaker for incubation. The hatching water is high-pressure sterilized natural seawater with an appropriate amount of penicillin and streptomycin added; collect the stimulated Cryptocaryon larvae liquid within 2 hours of hatching, mix thoroughly, take 50 μL of the larvae, and add 10 μL of formaldehyde to kill the larvae. Count under a microscope, and record the live worm concentration in the larva liquid.
[0028] 2. Domestication and infection of pomfret seedlings
[0029]Purchase about 12g of egg-shaped pomfret seedlings, and carry out trial breeding and domestication of the fry 2 weeks before infection until their eating and activities are normal. During...
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