Production method for cryptocaryon irritant prevention trachinotus ovatus seedling

A technology to stimulate Cryptocaryonia and pomfret, which is applied in the production of disease-resistant fry and anti-stimulatory Cryptocaryon fry, can solve the problem that there is no detailed study on the impact of seawater fish's immune protection, and the effective immune protection period is only limited. It can reach 3 months and other problems, and achieve the effect of long effective immune protection period, simple and easy operation, and reduced mortality

Inactive Publication Date: 2016-10-12
SOUTH CHINA AGRI UNIV +1
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Problems solved by technology

In recent years, researchers have carried out some basic research and applied research on the immune control of Cryptocaryon stimuli, and found that the larvae of Cryptocaryon stimuli can be used to infect seawater fish such as grouper and pomfret through the body surface, which can reach 80%. The above immu

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  • Production method for cryptocaryon irritant prevention trachinotus ovatus seedling
  • Production method for cryptocaryon irritant prevention trachinotus ovatus seedling

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Embodiment Construction

[0026] 1. Stimulation of live Cryptocaryon larvae collection and concentration calculation

[0027] Use a special Cryptocaryon stimuli subculture system to subculture and collect Cryptocaryon stimuli. The subculture host is Pomfret ovata. Collect well-developed Cryptocaryon stimuli cysts and place them in a sterilized clean beaker for incubation. The hatching water is high-pressure sterilized natural seawater with an appropriate amount of penicillin and streptomycin added; collect the stimulated Cryptocaryon larvae liquid within 2 hours of hatching, mix thoroughly, take 50 μL of the larvae, and add 10 μL of formaldehyde to kill the larvae. Count under a microscope, and record the live worm concentration in the larva liquid.

[0028] 2. Domestication and infection of pomfret seedlings

[0029]Purchase about 12g of egg-shaped pomfret seedlings, and carry out trial breeding and domestication of the fry 2 weeks before infection until their eating and activities are normal. During...

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Abstract

The invention relates to a production method for cryptocaryon irritant prevention trachinotus ovatus seedlings, and belongs to the field of disease resistance fry production, and specifically relates to the field of cryptocaryon irritant prevention fry production. A method of varying temperature infection and varying temperature culture is firstly used to produce disease resistance trachinotus ovatus seedlings. Water temperature in infection and cultivation water temperature in 10 days after infection keep certain temperature difference, so as to enhance immune effect of cryptocaryon irritant live larva, to further combine with rotational culture. The method obtains cryptocaryon irritant prevention trachinotus ovatus seedlings in a relatively long effective immune protection period. In the eighth month after immunity, the seedlings still have relative protection rate of 52%, and in the ninth month, the seedlings still have relative protection rate of 28%.

Description

technical field [0001] The technology relates to a production method of anti-stimulant Cryptocaryon ovata pomfret fry, belongs to the field of disease-resistant fry production, specifically the field of anti-stimulant Cryptocaryon fry production. Background technique [0002] Oval pomfret (Trachinotus ovatus), belonging to the family Trevalidae and the genus Pompano, is a warm-water pelagic fish with migratory and gregarious habits. It grows fast, eats simple food, and has delicious meat. It has become an important breeding species in the coastal areas of South China. Variety. [0003] Cryptocaryon irritans, commonly known as marine fish white spot disease, is a deadly parasitic disease caused by an obligate parasitic ciliate of marine fish——Cryptocaryon irritans (Colorni and Burgess, 1997) . In recent years, Cryptocaryoniasis has become one of the most serious parasitic diseases in my country, causing great harm to seawater fishery (Luo et al., 2008), and is listed as one...

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Application Information

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IPC IPC(8): A01K61/00A01K67/033
CPCA01K61/00A01K67/033Y02A40/81
Inventor 但学明李安兴李言伟莫泽权
Owner SOUTH CHINA AGRI UNIV
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