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Cryptocaryon irritans Brown prevention large yellow croaker fry production method

A technology that stimulates Cryptocaryonia and its production method is applied in the production of disease-resistant fry and anti-stimulatory Cryptocaryon fry. It can solve the problem that the effective immune protection period can only reach 3 months, and there is no detailed study on the immune protection of seawater fish. In order to achieve the effects of long period of effective immune protection, simple operation and reduced mortality

Inactive Publication Date: 2016-09-21
SOUTH CHINA AGRI UNIV +1
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  • Abstract
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AI Technical Summary

Problems solved by technology

In recent years, researchers have carried out some basic research and applied research on the immune control of Cryptocaryon stimuli, and found that the larvae of Cryptocaryon stimuli can be used to infect seawater fish such as grouper and pomfret through the body surface, which can reach 80%. The above immune protection rate (Luo et al, 2007; Dan Xueming et al, 2008; Yoshinaga et al, 1997), but the effective immune protection period of these experiments can only reach 3 months at most, and there is no detailed study on the effect of different temperatures on Effects of marine fish on acquired immune protection

Method used

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  • Cryptocaryon irritans Brown prevention large yellow croaker fry production method
  • Cryptocaryon irritans Brown prevention large yellow croaker fry production method

Examples

Experimental program
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Embodiment Construction

[0026] 1. Stimulate the collection and concentration calculation of live larvae of Cryptokaryon

[0027] Use a special Cryptocaryon irritans passaging system to passage and collect Cryptocaryon irritans. The passage host is the ovate pomfret. The well-developed Cryptocaryon irritans cysts are collected and incubated in a clean sterile beaker. The hatching water is autoclaved natural seawater with appropriate amount of penicillin and streptomycin; collect Cryptocaryon irritans larvae liquid within 2 hours of hatching, mix well, take 50μL insect liquid, add 10μL formaldehyde to kill the larvae, Count under a microscope and record the concentration of live insects in the larval fluid.

[0028] 2. Domestication and infection of large yellow croaker fry

[0029] Purchase about 10g of large yellow croaker fry, and conduct trial breeding and domestication of the fry 2 weeks before infection until they eat and move normally. During the entire breeding process, the rearing water is filtered ...

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Abstract

The invention discloses a cryptocaryon irritans Brown prevention large yellow croaker fry production method, and belongs to the field of disease-resistance fry production, specifically the field of cryptocaryon irritans Brown fry production. The method comprises the steps: firstly producing disease-resistance large yellow croaker fries in modes of variable temperature infection and variable temperature cultivation; secondly maintaining a certain temperature difference between the water temperature during infection and cultivation water temperature in ten days, thereby improving the immune effect cryptocaryon irritans Brown larvae, so as to combine with alternate cultivation, and to obtain the cryptocaryon irritans Brown prevention large yellow croaker fry with a longer effective immune protection period, wherein the large yellow croaker fry still has a 62% relative protection rate in an eighth month, and still has a 30% relative protection rate in a ninth month.

Description

Technical field [0001] The technology relates to a method for producing anti-irritant Cryptocarpidium large yellow croaker fry, which belongs to the field of disease-resistant fry production, and is specifically the production field of anti-irritant Cryptocaryon fry. Background technique [0002] Large yellow croaker (Larimichthys crocea), belonging to the order Perciformes, Tothocephalidae, and yellow crocea, is a warm-temperate bottom offshore fish. It is one of the traditional "four marine products" in China and is known as the "Chinese national fish". At present, it is the most important mariculture fish in Fujian and Zhejiang. [0003] Cryptocaryon irritans, commonly known as marine fish white spot disease, is a lethal parasitic disease caused by a kind of obligate parasitic ciliates of marine fishes, Cryptocaryon irritans (Colorni and Burgess, 1997) . In recent years, Cryptocaryon irritans has become one of the most serious parasitic diseases in my country, causing great ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCA01K61/00Y02A40/81
Inventor 但学明李安兴李言伟莫泽权
Owner SOUTH CHINA AGRI UNIV
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