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Cultivation of nucleus pearl by using free cell implantation method

A technology of free cells and implantation, which is applied in the field of nucleated pearl cultivation, and can solve problems such as uneven distribution and generation of dirt beads

Inactive Publication Date: 2007-05-23
施志仪
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention needs to solve the problem of non-uniform distribution of nacre in nucleated pearl breeding pearls, resulting in various defective pearls such as dirty beads and tail beads, and provides a method for improving the quality of pearl breeding

Method used

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Embodiment Construction

[0007] The present invention adopts healthy and energetic young mussels within one age or less, and temporarily raises them in bromogeramine with a concentration of 0.01% for 2 to 3 hours, cuts the adductor muscle of the mussels with a scalpel, and rinses the mantle repeatedly with distilled water. And cut off the edge of the mantle color line, wipe off the mucus with alcohol cotton ball, and then separate the outer skin of the mantle of the mussel by tearing the film, soak it in 0.01% potassium permanganate solution for disinfection for 10 minutes, and then put it in the ultra-clean Clean the workbench with 1000U / mL penicillin + 1000U / mL streptomycin cleaning solution, and then rinse it repeatedly with sterilized balanced salt solution D-Hanks for 4-5 times, and cut the outer skin of the mantle to 1mm 2 After digesting the small pieces in 0.25% trypsin at 37°C for 30min, filter with 300-mesh silk screen to remove excess tissue pieces, stop trypsinization and disperse cells wit...

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Abstract

The invention relates to a free cell implant method used to cultivate corn pearl, wherein it comprises that using the sheath skin of clamshell, uses enzymolysis method to obtain the skin cell, adjusts the cell density to 5*105-1*106, and puts the pearl corn treated with 0.1mg / ml polylysine into the cell suspension to be cultivated, therefore the cell will adhere the surface of pearl corn; implants 0.2mL free cell suspension into the clamshell, implants the corn with cells into the clamshell, adds 0.2mL cell suspension, suspends the clamshell 20cm under water and cultivates.

Description

Technical field: [0001] The invention relates to a method for cultivating nucleated pearls. Background technique [0002] Pearl is a secreted product of the pearl sac in the pearl oyster, and the pearl sac is formed by the rapid proliferation of epithelial cells of the mantle of the pearl oyster and gradually surrounds the stimulus after being stimulated. Therefore, the mantle of the pearl oyster plays an important role in the formation of pearls. Artificial pearl cultivation technology is to implant the mantle tissue block of pearl oyster alone or together with the bead core made of shell or other materials into the body of pearl oyster to form seedless pearls or nucleated pearls. Compared with nucleated pearls, the quality of artificial non-nucleated pearls is shorter and the quality of nucleated pearls is better. However, the direct insertion method used in the early artificial nucleated pearls is difficult to form pearl sacs, and there are very few beads, which is not co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 施志仪杨显祥李巍吴超群
Owner 施志仪
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