Active amino-modified substrate for microarray and preparation method of active amino-modified substrate
A technology of amino modification and microarray, which is applied in the fields of medicine and biology to solve the problems of low sensitivity, improved fixation rate and poor stability
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Embodiment 1
[0035] A method for preparing a substrate modified with active amino groups for a microarray, comprising the steps of:
[0036] Step (1), pretreatment of the glass substrate: soak the glass substrate in a mixed solution of concentrated sulfuric acid:hydrogen peroxide = 7:3 for 13 h, and remove the residual concentrated sulfuric acid and hydrogen peroxide mixture on the surface of the glass substrate with Rinse twice with deionized water, each time for 5 minutes, and finally spin dry to obtain a pretreated glass substrate;
[0037] Step (2), aminosilanization of the glass substrate (introduction of active amino groups): the pretreated glass substrate obtained in step (1) was mixed with 1.0% 3-aminopropyldiethoxy The silane solution is fully reacted to introduce active amino groups, and then the residual 3-aminopropyldiethoxysilane solution is cleaned with 60% ethanol, and finally dried to obtain a glass substrate with aminosilanized surface;
[0038] Step (3), poly-lysine coat...
Embodiment 2
[0040] A method for preparing a substrate modified with active amino groups for a microarray, comprising the steps of:
[0041] Step (1), pretreatment of the glass substrate: soak the glass substrate in a mixed solution of concentrated sulfuric acid: hydrogen peroxide = 7:3 for 18 h, remove the residual concentrated sulfuric acid and hydrogen peroxide mixture on the surface of the glass substrate with Wash with deionized water 5 times, each time for 5 minutes, and finally spin dry to obtain the pretreated glass substrate;
[0042]Step (2), aminosilanization of the glass substrate (introduction of active amino groups): the pretreated glass substrate obtained in step (1) was mixed with 3.0% 3-aminopropyldiethoxy The silane solution is fully reacted to introduce active amino groups, and then the remaining 3-aminopropyldiethoxysilane solution is cleaned with 80% ethanol, and finally dried to obtain a glass substrate with aminosilanized surface;
[0043] Step (3), poly-lysine coat...
Embodiment 3
[0045] A method for preparing a substrate modified with active amino groups for a microarray, comprising the steps of:
[0046] Step (1), pretreatment of the glass substrate: soak the glass substrate in a mixed solution of concentrated sulfuric acid: hydrogen peroxide = 7:3 for 15 h, and remove the residual concentrated sulfuric acid and hydrogen peroxide mixture on the surface of the glass substrate with Wash with deionized water for 3 times, each time for 4min, and finally spin dry to obtain the pretreated glass substrate;
[0047] Step (2), aminosilanization of the glass substrate (introduction of active amino groups): the pretreated glass substrate obtained in step (1) was mixed with 2.5% 3-aminopropyldiethoxy The silane solution is fully reacted to introduce active amino groups, and then the residual 3-aminopropyldiethoxysilane solution is cleaned with 70% ethanol, and finally dried to obtain a glass substrate with aminosilanized surface;
[0048] Step (3), poly-lysine c...
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Description
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Application Information
- IPC
- B01L3/00
- CPC
- B01L3/502707; B01L2300/0819; B01L2300/0822; B01L2300/12
- Inventors
- 周勇; 申友锋