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Preparation method of biologically active chronic wound auxiliary material and application thereof

A technology of biological activity and surface excipients, applied in medical science, bandages, etc., can solve the problems of multifunctional hydrogel dressings that have not been reported yet, and achieve chemical synthesis methods that are environmentally friendly, biocompatible, cheap and easy to obtain, and biocompatible good sex effect

Inactive Publication Date: 2019-10-18
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned multifunctional hydrogel dressings for diabetic wound healing have not been reported yet.

Method used

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  • Preparation method of biologically active chronic wound auxiliary material and application thereof
  • Preparation method of biologically active chronic wound auxiliary material and application thereof
  • Preparation method of biologically active chronic wound auxiliary material and application thereof

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preparation example Construction

[0042] like figure 1 Shown, preparation method of the present invention specifically comprises the following steps:

[0043] 1) Cationic functionalized polyether polymer synthesis. Completely dissolve polyether F127 with a molar ratio of 1:(8~12):(8~12), p-toluenesulfonyl chloride and triethylamine in 100~150mL of anhydrous dichloromethane, and react at room temperature under nitrogen for 18 ~24 hours later, the prepolymer was obtained by extraction and precipitation, and then the prepolymer and cationic polymer with a molar ratio of 1: (1.5~4.5) were completely dissolved in 100~150mL N,N-dimethylformamide, After stirring at 60-65°C for 48-60 hours, the reaction product was dialyzed and purified in deionized water for 2-3 days, and freeze-dried for later use;

[0044] Preferably, the cationic functionalized polyether F127 in step 1) is ε-polylysine or polyethyleneimine functionalized polyether F127, wherein the molar ratio of cationic polymer to F127 is 2.5:1.

[0045] 2) S...

Embodiment 1

[0056] 1) Preparation of F127-PEI polymer: Dissolve polyether F127 with a molar ratio of 1:10:10, p-toluenesulfonyl chloride and triethylamine in 100 mL of anhydrous dichloromethane, and react at room temperature for 24 Hours later, the prepolymer was obtained by extraction and precipitation, and then the prepolymer and polyethyleneimine with a molar ratio of 1:2.5 were completely dissolved in 100mL N,N-dimethylformamide, and stirred at 60°C for 48 hours Finally, the reaction product F127-PEI was dialyzed and purified in deionized water for 3 days, and freeze-dried for later use;

[0057] 2) Preparation of APu polymer: Dissolve pullulan and sodium periodate at a molar ratio of 1:1 in 150mL deionized water, stir at room temperature for 12 hours in the dark; add sodium periodate Ethylene glycol with a molar ratio of 2:1 terminated the reaction. After stirring for 2 hours at room temperature, the reaction product APu was dialyzed and purified in deionized water for 3 days, and fr...

Embodiment 2

[0061] 1) Preparation of F127-PEI polymer: Dissolve polyether F127 with a molar ratio of 1:10:10, p-toluenesulfonyl chloride and triethylamine in 100 mL of anhydrous dichloromethane, and react at room temperature for 24 Hours later, the prepolymer was obtained by extraction and precipitation, and then the prepolymer and polyethyleneimine with a molar ratio of 1:2.5 were completely dissolved in 100mL N,N-dimethylformamide, and stirred at 60°C for 48 hours Finally, the reaction product F127-PEI was dialyzed and purified in deionized water for 3 days, and freeze-dried for later use;

[0062] 2) Preparation of APu polymer: Dissolve pullulan and sodium periodate at a molar ratio of 1:1 in 150mL deionized water, stir at room temperature for 12 hours in the dark; add sodium periodate Ethylene glycol with a molar ratio of 2:1 terminated the reaction. After stirring for 2 hours at room temperature, the reaction product APu was dialyzed and purified in deionized water for 3 days, and fr...

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Abstract

The invention discloses a preparation method of a biologically active chronic wound auxiliary material and an application thereof. The method firstly prepares an epsilon-polylysine or polyethyleneimine functionalized polyether F127 polymer, and then oxidizes sodium alginate or pullulan by sodium periodate to obtain polysaccharide having an aldehyde group, a hydrogel having a three-dimensional network structure is formed by reacting a Schiff base between the cationic functionalized F127 polymer and the aldehyde-based polysaccharide, the exosomes are loaded into the hydrogel by electrostatic interaction with the cationic polymer, and the bioactive antibacterial and photodamage-resistant chronic wound auxiliary material can be prepared. The bioactive antibacterial and photodamage-resistant chronic wound auxiliary material prepared by the method has excellent mechanical properties such as temperature sensitivity, injectability and self healing, good antibacterial, anti-light damage and promotion of wound healing. Therefore, the hydrogel has the good application prospect in antibacterial, anti-light damage and promoting chronic wound healing.

Description

technical field [0001] The invention belongs to the technical field of degradable biomedical materials, and in particular relates to a preparation method and application of a bioactive chronic wound auxiliary material. Background technique [0002] The high incidence of diabetes has become a serious problem that threatens the health and quality of life of patients. Hyperglycemia-induced microvascular dysfunction, one of the main causes of diabetic complications, often leads to wound ischemia and delayed healing in patients. Wound healing generally needs to go through four phases, namely, coagulation phase, inflammation phase, cell proliferation phase, and new tissue remodeling phase, and also requires nutrients and oxygen provided by blood vessels. Therefore, enhancing angiogenesis and revascularization in diabetic wounds is crucial to accelerate chronic wound healing. Traditional clinical treatments for diabetic wounds are glycemic control, surgical debridement, grafting,...

Claims

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Application Information

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IPC IPC(8): A61L26/00C08J3/075C08G81/02C08B37/00C08L5/04C08L5/02
CPCA61L26/0052A61L26/0066A61L26/008A61L26/009A61L2300/30A61L2300/404A61L2300/412C08B37/0018C08B37/0084C08G81/025C08G81/028C08J3/075C08J2387/00C08J2405/02C08J2405/04C08L5/04C08L5/02C08L87/00
Inventor 雷波王敏
Owner XI AN JIAOTONG UNIV
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