The invention discloses a polypeptide freeze-dried powder mask composition, and a preparation method and use method thereof, and relates to the technical field of masks. According to the technical scheme, the polypeptide freeze-dried powder mask composition consists of polypeptide freeze-dried powder, a freeze-drying solvent, mask liquid and mask cloth. The polypeptide freeze-dried powder is prepared from the following raw materials: mannitol, trehalose, glycine, creatine, alpha-arbutin, tranexamic acid, ascorbic acid, mandelic acid, a cortex magnoliae officinalis extract, nonapeptide-1, oligopeptide-5, oligopeptide-1, lecithin and the balance of water. The mask liquid is prepared from the following raw materials: polyglycerol-10, a plant soothing agent, a dendrobium extract, argania spinosa kernel oil, a preservative composition, carbomer, triethanolamine, a sugarcane extract, hydroxyethyl cellulose, a tremella extract, hydrogenated castor oil, Damascus rose essential oil and the balance of Damascus rose hydrolat. The mask provided by the invention has the advantages of having high active ingredient content and having moisturizing, soothing, whitening and repairing functions.