Probiotic drop for improving allergic constitution
A technology of probiotics and allergies, applied in the field of medicine and biology, can solve the problems of low efficiency of homogenizer, easy side effects, poor emulsification effect, etc.
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Embodiment 1
[0077] S1:
[0078] (1) selection medium, respectively MRS medium, L7-13 medium, PTYG medium and SL medium, sterilized;
[0079] (2) Select strains, respectively Lactobacillus casei, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus acidophilus, Bifidobacterium longum, Lactobacillus plantarum, Lactobacillus fermentum, use 25- Dilute with warm water at 40°C;
[0080] (3) Aseptically add bovine serum and yeast glucan as growth-promoting factors to promote the growth and reproduction of probiotics;
[0081] (4) Adding a fiber material 80 times the volume of the culture medium as a porous material to facilitate high-density cultivation of probiotics;
[0082] (5) Inoculate Lactobacillus casei, Lactobacillus paracasei, Lactobacillus reuteri, Lactobacillus acidophilus and Lactobacillus plantarum in MRS medium, inoculate Lactobacillus rhamnosus in L7-13 medium, and cultivate in PTYG Bifidobacterium longum was inoculated in the medium, fermented...
Embodiment 2
[0097] S1:
[0098] (1) selection medium, respectively MRS medium, L7-13 medium, PTYG medium and SL medium, sterilized;
[0099] (2) Select strains, respectively Lactobacillus casei, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus acidophilus, Bifidobacterium longum, Lactobacillus plantarum, Lactobacillus fermentum, use 25- Dilute with warm water at 40°C;
[0100] (3) Aseptically add bovine serum and yeast glucan as growth-promoting factors to promote the growth and reproduction of probiotics;
[0101] (4) Adding a fiber material with 100 times the volume of the culture medium as a porous material, thereby facilitating high-density cultivation of probiotics;
[0102] (5) Inoculate Lactobacillus casei, Lactobacillus paracasei, Lactobacillus reuteri, Lactobacillus acidophilus and Lactobacillus plantarum in MRS medium, inoculate Lactobacillus rhamnosus in L7-13 medium, and cultivate in PTYG Bifidobacterium longum was inoculated in the med...
Embodiment 3
[0117] S1:
[0118] (1) selection medium, respectively MRS medium, L7-13 medium, PTYG medium and SL medium, sterilized;
[0119] (2) Select strains, respectively Lactobacillus casei, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus reuteri, Lactobacillus acidophilus, Bifidobacterium longum, Lactobacillus plantarum, Lactobacillus fermentum, use 25- Dilute with warm water at 40°C;
[0120] (3) Aseptically add bovine serum and yeast glucan as growth-promoting factors to promote the growth and reproduction of probiotics;
[0121] (4) Adding a fiber material with 120 times the volume of the culture medium as a porous material, thereby facilitating the high-density cultivation of probiotics;
[0122] (5) Inoculate Lactobacillus casei, Lactobacillus paracasei, Lactobacillus reuteri, Lactobacillus acidophilus and Lactobacillus plantarum in MRS medium, inoculate Lactobacillus rhamnosus in L7-13 medium, and cultivate in PTYG Bifidobacterium longum was inoculated in the...
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