90 results about "Gene vector" patented technology

The invention discloses a tryptophan synthase mutant as well as a coding gene, a vector, recombinant bacteria and application thereof. Comprising the following steps: carrying out error-prone PCR (Polymerase Chain Reaction) on a coding gene trpBA of TrpS from EscherichiaoliK12MG1655, screening mutants by combining high-throughput screening, and finding that the affinity of the TrpS to a substrate indole can be effectively improved by mutating 114-site Gln of a beta subunit of the TrpS into Met and mutating 84-site Gly into Ser, so that the synthesis of L-tryptophan is promoted. The efficiency of the TrpS for converting indole and L-serine into L-tryptophan is higher due to the improvement of the affinity of indole. A new idea is provided for efficient production of L-tryptophan in the fermentation industry.

The invention provides a tumor cell vaccine as well as a preparation method and application thereof. The preparation method comprises the following steps: acquiring and culturing tumor cells; enabling the cell membrane of the tumor cell to express a targeting antibody by utilizing a gene vector infection or gene editing technology, and enabling the targeting antibody to be used for enabling the tumor vaccine to be combined with the dendritic cell; performing overexpression of tumor specific protein on the cell membrane surface of the tumor cell by using a gene vector infection or gene editing technology; a nucleic acid substance of a target protein is wrapped by lipid nanoparticles to form a vaccine framework, and the nucleic acid substance can enable target cells to express directional chemotactic molecules; wherein the directed chemotactic molecule is used for transferring the dendritic cells to lymph nodes; the target vaccine is obtained by wrapping the vaccine framework with the cell membranes of the tumor cells, lymph node homing of the dendritic cells can be promoted, and the antigen presentation efficiency is increased and the immune effect is enhanced through tumor specific protein carried by the target vaccine and assisted by an immunologic adjuvant.

The invention belongs to the field of genetic engineering and resource recycling, and relates to a ferrous oxide recombinant bacterium, a recombination method and application of the ferrous oxide recombinant bacterium in lithium battery metal recovery. The ferrous oxide recombinant bacterium is obtained by taking thiobacillus ferrooxidans as an original strain through genetic engineering modification; comprising thiobacillus ferrooxidans, an exogenously introduced nickel / cobalt efflux protein RcnA gene, a vector pJRD215 and donor bacteria, and the nickel / cobalt efflux protein RcnA gene is introduced into the vector pJRD215, driven to express and then introduced into the donor bacteria to express. The application of the constructed and prepared ferrous oxide recombinant bacteria in lithium battery metal recovery is used for improving the leaching efficiency of metal ions Co < + > or / and Li < + > or / and Ni < + > in lithium battery powder; and the method shows good adaptability and stability under different substrate concentrations, and provides a key experimental support and an important experimental basis for application of a microbial enhanced leaching technology in the field of waste lithium battery resource utilization.

The invention relates to the technical field of artificial intelligence-driven digital marketing, in particular to an AI-based new media intelligent marketing method, which comprises the following steps of: 1, carrying out cross-platform user behavior fusion modeling; 2, constructing a multi-modal content gene pool: analyzing a visual focus area and a text emotional tendency of historical content, and generating a content gene vector; 3, dynamic content generation and real-time optimization, wherein initial marketing content is generated according to the space-time behavior matrix retrieval gene bank; user interaction behaviors are monitored in real time, elements in a visual focus area are dynamically replaced, and the emotion intensity of the copywriting is adjusted; and step 4, closed-loop strategy optimization: a dynamic effect index is calculated by fusing multi-platform indexes, a gene variation mechanism is triggered when the index continuously decreases, and cross-platform compensation content is generated based on failed user behaviors. By analyzing the cross-platform behavior and the content preference of the user, the user demand can be accurately predicted, and the content more conforming to the personalized preference of the user is provided.

The invention discloses basic amino acid modified 3S-PCL as well as a preparation method and application thereof, and relates to the technical field of biological medicines. The basic amino acid modified 3S-PCL has a structural formula shown in the specification, wherein R is alkaline amino acid; m, m1 and m2 are all positive integers, and m, m1 and m2 are greater than or equal to 1. According to the invention, a basic amino acid modified three-arm polycaprolactone (3S-PCL) material is designed and synthesized, and the basic amino acid modified three-arm polycaprolactone (3S-PCL) material has good biocompatibility and can be used for lipid nanoparticle nucleic acid delivery instead of a cationic liposome. The preparation method of the basic amino acid modified 3S-PCL material is simple and rapid, and is especially suitable for industrial production. The lipid nanoparticles prepared by using the basic amino acid modified 3S-PCL material as a gene vector can realize efficient delivery of nucleic acid drugs.

The invention provides a territorial space planning data monitoring evaluation method and system, and relates to the technical field of territorial space planning. The territorial space planning data monitoring and evaluation method specifically comprises the following steps: S1, inputting multi-source territorial space data into a space DNA encoder, and generating three types of gene vectors, namely a topographic gene T-Gene, an ecological gene E-Gene and a construction gene U-Gene, by taking a 30m * 30m grid as a basic unit. Territorial space evolution is converted into a digital model, and a traditional planning problem is solved; the space DNA folding improves the early warning sensitivity; a self-repairing strategy is accurately released; perspective of conflict hotspots is realized through a three-dimensional dynamic instrument panel; autonomous iterative optimization of the system provides a scientific operable normal form for sustainable development of territorial space.

The invention relates to the technical field of gene vectors, and discloses a multi-target multi-gene stable silencing vector and an application thereof in treating diabetes mellitus, and a multi-gene multi-target gene modification combined element is safely and efficiently integrated at a gene safety parking point of a second intron region of a CCR5 gene in a genome in a reverse fixed point manner by using a non-viral vector system; therefore, the effect of continuously silencing expression of a plurality of target genes such as MSTN, GRB10 and PTBP1 for a long time is achieved, and aging of mesenchymal stem cells cultured in vitro is remarkably inhibited. When the mesenchymal stem cells modified by the multi-target multi-gene stable silencing vector are infused, the activity is high, the chronic inflammatory microenvironment is effectively inhibited, and the senescence process is delayed; moreover, proliferation and differentiation of pancreatic beta cells are promoted, insulin secretion is enhanced, insulin resistance is effectively resisted, and diseases such as diabetes mellitus are prevented.

This invention relates to the field of genetic engineering, specifically to an HSL mutant, gene, vector, cell, composition, and its applications. The mutant is selected from... Os HSL4 enzyme, Sb HSL1 enzyme, Zm HSL1A enzyme, Hv HSL6D enzyme and Zm A mutant of at least one of the HSL1B enzymes. The HSL mutants, genes, vectors, cells, and compositions provided by this invention can improve plant resistance to HPPD inhibitors.

The application relates to the technical field of biological macromolecular materials, in particular to a preparation method and application of a cationic polymer gene carrier with a fluorinated end group. The cationic polymer gene carrier with a fluorinated end group has the following structure. The fluorinated initiator is used to introduce fluorinated groups in one step to initiate the synthesis of linear polyethylene imine F-PEI with various molecular weights, the introduction of the fluorinated groups improves the gene transfection efficiency, and high-efficiency DNA transfection is realized. The gene carrier can achieve high-efficiency transfection effect in the cell transfection process, the transfection process has small toxicity to cells, gene molecules can be effectively and safely delivered into cells, and the gene carrier has the advantages of high efficiency, low toxicity, low price, simple synthesis and the like. The application has the advantages of simple reaction, easily-obtained raw materials, low preparation cost, easy control of molecular weight, high yield, simple post-treatment and suitability for large-scale production.

This invention discloses proteins related to the regulation of lily bulblet formation, their encoding genes, and their applications. The lily bulblet formation regulation proteins disclosed in this invention are either A1) or A2): A1) is a protein with the amino acid sequence of Sequence 2; A2) is a protein with one or more amino acid residues substituted and / or deleted and / or added to the amino acid sequence shown in Sequence 2 in the sequence listing, and has the same function as the protein shown in Sequence 2. This invention isolated and identified a LiARF6 gene related to bulblet formation from scale cuttings in 'Siberian' lily, and improved it using VIGS technology. By infecting bulbs with a bacterial solution containing this gene vector, the effect of enhancing the formation of bulblets during scale cutting was successfully achieved, providing a new method for promoting lily scale cutting propagation.

The invention discloses a redox sensitive cationic polymer gene vector as well as preparation and application thereof. According to the invention, hydrophobic small molecules with oxidation / reduction responsive S-S bonds are adopted to carry out side chain modification on polyglycidyl amine, and the cationic polymer gene delivery carrier material with specific tumor microenvironment response capability is prepared. The material has good biocompatibility, and can effectively load siRNA molecules and form siRNA compound nanoparticles with good stability and excellent gene transfection capacity. The compound nanoparticles can be effectively absorbed by cells, and can effectively respond to an oxidation / reduction tumor microenvironment to realize targeted release of siRNA. The oxidation / reduction response cationic polymer gene vector provided by the invention has a relatively great clinical application prospect.

This invention discloses a basic amino acid-modified 3S-PCL, its preparation method, and its applications, relating to the field of biomedical technology. The structural formula of the basic amino acid-modified 3S-PCL is as follows: [Structure formula would be inserted here]; where R is a basic amino acid; m, m1, and m2 are all positive integers, and m, m1, and m2 ≥ 1. This invention designs and synthesizes a basic amino acid-modified three-armed polycaprolactone (3S-PCL) material, which exhibits good biocompatibility and can replace cationic liposomes for nucleic acid delivery via lipid nanoparticles. The preparation method of this basic amino acid-modified 3S-PCL material is simple and rapid, particularly suitable for industrial production. Lipid nanoparticles prepared using this basic amino acid-modified 3S-PCL material as a gene carrier can achieve highly efficient delivery of nucleic acid drugs.

The invention relates to a gene vector for CSF1R (Classical Sequence Factor 1R) related white matter encephalopathy as well as a construction method and application of the gene vector. The gene vector is AAV.MG1.1-Trem2. The gene vector disclosed by the invention can be used for increasing the expression content of the Trem2 of the microglial cells and compensating the dysfunction of the pathogenic gene CSF1R, so that the microglial cells can exert normal functions.

The invention discloses a PID effect accelerated aging prediction method for a component, and aims to solve the problems of individuation and mechanism uncertainty in component aging prediction. The method comprises the following steps: firstly, establishing a component gene vector containing multi-source heterogeneous attributes for each component so as to instantiate a digital twin; secondly, constructing a shared causal guided generative model taking a physical causal map as a structural constraint as a prediction core; in dynamic operation, the system performs probabilistic path prediction on a single twinborn body, and dynamically calibrates the state of the twinborn body by using new measured data through a data assimilation technology. Furthermore, the system inversely optimizes the physical causal atlas by using a structure learning algorithm by aggregating evolution data of the whole twinborn cluster, so as to realize iterative upgrade of the prediction core. According to the method, individual self-adaptive calibration and swarm intelligent evolution are combined, so that the system has the capability of discovering and perfecting physical laws from data, and the accuracy and robustness of long-term prediction are remarkably improved.

The application discloses a single-cell transcriptome data pseudo-time trajectory analysis method and a computer system, which comprises the following steps: 1) calculating a gene explicit comparison advantage matrix; 2) obtaining a gene similarity matrix by similarity and constructing a gene network; 3) taking an initial node in the gene network, starting random walking from the initial node, recording each gene walked through to form a gene text composed of gene sequences; 4) converting the gene text into a gene word vector; 5) adding all single-cell expressed gene vectors with expression as weight to form a sum vector as a word vector representation of the single cell in the gene space; and 6) visualizing all cell vector representations to obtain an embryo cell development pseudo-time trajectory result. The application provides an analysis basis for identifying different rare cell subtypes in tissues and variant genes of different cell subtypes and has a wide and important application prospect in the fields of tumors, developmental biology and life science.

The invention relates to the field of gene vector construction, in particular to construction and application of a gene vector integrating fluorescent screening and self-deletion functions, the gene vector comprises a conditional knock-out (cKO) vector and a gene knock-in (KI) vector which are both integrated with a Dre-Rox mediated self-deletion module and a fluorescent screening module, the construction method comprises the following steps: carrying out HindIII / EcoRI double enzyme digestion on a pUC19 vector, carrying out homologous recombination amplification on a target fragment, carrying out connection transformation and screening verification to obtain a qualified vector; the vector is applied to preparation of cKO / KI gene modified mice, and efficient screening of positive individuals is realized through fluorescence preliminary screening, genotype identification and fluorescence quenching detection. The positive screening workload and cost can be reduced by 70% or above, non-target elements are accurately cut off, interference is avoided, time-space accurate regulation and control are achieved, carrier construction is easy and convenient, repeatability is high, and the method is suitable for gene function research, disease model construction and drug target verification.

The invention discloses a regional scale city form multi-objective optimization method and system based on proxy model assistance, and the method comprises the steps: carrying out the discretization of a city region, obtaining a plurality of grid units, and obtaining a global gene vector and a local gene vector of each grid unit; decoding the gene vector to obtain an urban morphological feature matrix; obtaining the total energy demand of the region by using the proxy model; constructing a multi-objective optimization model by taking minimization of the total energy demand of the region and maximization of the development intensity as objective functions; on the basis of a multi-objective evolutionary algorithm, population individuals are converted into a feature matrix, the individual fitness is evaluated by utilizing an agent model, iterative search is performed on gene vectors, and a Pareto optimal solution set is obtained and serves as an urban form optimization scheme. According to the method, through an innovative hierarchical gene coding technology and an efficient proxy model evaluation technology, on the premise that the planning feasibility is guaranteed, the tradeoff relation between the regional scale urban form and the energy performance is rapidly, accurately and widely explored.

This invention relates to the field of gene vector technology, and more particularly to an ionizable comb-like polymer, its preparation method, and its applications. This comb-like polymer is a block polymer, comprising at least one block unit. Each block unit includes a first block, a second block, and a third block linked by covalent bonds. It exhibits high molecular flexibility and readily binds to nucleic acid-like substances to form polymer / nucleic acid complexes. Due to the presence of easily degradable intracellular glycosidic bonds in its structure, the polymer demonstrates high transfection efficiency. Furthermore, cell experiments show that the polymer / nucleic acid complex exhibits low toxicity to cells and significantly improves cell survival compared to the positive control. Therefore, it holds promise for widespread application in cell transfection. The preparation method provided by this invention utilizes a redox amination tandem reaction to directly prepare the target product, eliminating the need for intermediate product purification processes. This simple process facilitates large-scale production.

The present application relates to the technical field of recombinant protein technology, and particularly relates to a high-salt-tolerant amidase, gene, vector, recombinant bacteria and application, wherein the amino acid sequence of the amidase is shown as SEQ ID NO. 1. The amidase has extremely high salt tolerance and good ethanol tolerance. The recombinant amidase prepared by the present application lays a foundation for future realization of elimination of ethyl carbamate in high-salt fermented food and alcoholic beverages and realization of industrial application of ethyl carbamate hydrolytic enzyme, and has great economic and social benefits.

The invention provides application of a transcription factor OsNAC10 in improvement of rice phosphorus utilization and yield. The application comprises any one of the following applications of a rice OsNAC10 gene, the transcription factor OsNAC10 and a recombinant vector containing the OsNAC10 gene: A, improvement of rice phosphorus utilization; b, preparing a product for improving phosphorus utilization of rice; c, increasing the rice yield; and D, preparing a product for improving the rice yield, wherein the nucleotide sequence of the rice OsNAC10 gene is as shown in SEQ ID NO: 1. The invention provides a new theoretical basis and genetic resources for the cultivation of high-efficiency phosphorus varieties of rice.

The application provides sgRNA which specifically targets the Rosa26 safe site of a cashmere goat and application of using CRISPR / Cas9 to complete site-directed knockout and site-directed integration of an EGFP gene. The application firstly uses a bioinformatics method to predict the complete sequence of the Rosa26 site, then designs two sgRNA aiming at the site, constructs a targeting vector based on the CRISPR / Cas9 system, verifies the guiding efficiency of the sgRNA, simultaneously constructs a homologous integration EGFP gene vector, co-transfects the sgRNA and the homologous integration vector into cashmere goat fetal fibroblasts, and obtains a cell strain of the Rosa26 site site-directed integration of the EGFP gene. The efficiency of the sgRNA of the application in specifically guiding Cas9 to cut the Rosa26 site reaches about 40%, effectively reduces the off-target phenomenon existing in the CRISPR / Cas9 system, and further reduces the mutation of non-target gene sequences caused by non-specific cutting. The Cas9 / gRNA expression vector can realize the specific knockout or knock-in of the Rosa26 site at the cell, embryo or even individual level, so as to study the expression of specific genes and provide technical support for the cultivation of new goat breeds.

The present application relates to the technical field of medicine, and in particular to a nonionic gene vector on the basis of a hydrogen bond donor group, and a preparation method therefor and the use thereof. The gene vector loads a gene drug via a group that functions as a hydrogen bond and is formed by a hydrogen bond donor gene and a gene nucleoside or phosphate, and comprises a hydrogen bond donor group being a nonionic lipid and a hydrogen bond donor nonionic polymer. The hydrogen bond donor group mainly comprises thiourea, polyphenol, hydroxyl, urea, thymine, uracil, cytosine, adenine and guanine. The delivery system of the present application can be easily prepared, and loading can be achieved by means of a simple method of blending with mRNA at room temperature, which avoids the previous problems, such as the complex preparation process. In addition, the delivery system of the present invention has the characteristic of being degradable and has good biosafety.

The application discloses a robot morphology directional evolution method based on environment perception and a related device, and relates to the technical field of robot morphology evolution. The method comprises the following steps: initializing a morphology gene vector and a control strategy of a robot; sampling and executing an action strategy according to the control strategy and environment perception information, and then calculating a system benefit value; judging whether the morphology gene vector of the robot is adapted to a current environment according to the environment perception information and the system benefit value; when the judgment result is yes, performing a mutation operation on the morphology gene vector of the robot based on a morphology mutation matrix; otherwise, jumping back to the step of sampling and executing the action strategy until a maximum iteration number is reached. The application quantifies the cumulative stimulation of environmental changes on the robot through an environment perception function to determine whether the robot needs to perform morphology evolution, and guides the direction of morphology evolution of the robot in different environments through the morphology mutation matrix, thereby accelerating the morphology evolution process of the robot.

This invention belongs to the field of biodetection technology, specifically relating to a material for detecting anti-NF155 antibodies and its preparation method. This invention constructs an expression plasmid capable of highly expressing the NF155 antigen, transfects it into HEK293T cells, and modifies the pCDH vector with enhancers during construction. Subsequently, a modified cell culture protocol is used to obtain a material for detecting anti-NF155 antibodies based on a cell immunofluorescence methodology. This invention uses a cell immunofluorescence methodology to maximize the preservation of the antigen's spatial conformation through cell expression and antigen immobilization, preventing false negatives due to changes in binding sites. This invention selects the lentiviral vector pCDH to avoid the problem of non-specific binding that is difficult to distinguish, as the proteins expressed by common target gene vectors such as pcDNA3.1 may themselves contain antibody binding sites. This improves detection specificity and solves the problem of false negatives.

The invention belongs to the technical field of gene engineering, and particularly relates to an NRPS gene, a vector and construction and preparation of a high-yield cyclic dipeptide compound recombinant strain of the NRPS gene. According to the invention, an NRPS core gene is excavated from a sea squirt-derived fungus Diapothesp.SYSU-MS4722, a recombinant strain is constructed by using the gene and using Aspergillus oryzae as a heterologous expression host, the cyclic dipeptide natural product cyclo (Phe-Ala) is successfully separated, and the yield can reach 110 mg / L, which is increased by 20 times compared with the yield of cyclo (Phe-Ala) in reported marine bacteria Pseudomonasputida. Therefore, the aspergillus oryzae recombinant strain disclosed by the invention can be used for efficiently preparing the cyclic dipeptide compound cyclo (Phe-Ala) and has an important application value.

The application provides a preparation method and application of a gold / fluorinated PEI composite gene carrier. The gold / fluorinated PEI composite gene carrier is a thiolated fluorine-containing polyethylene imine modified by gold nanoparticles, and is referred to as F-LPEI-SH-Au. Fluorine groups are modified on one end of the linear polyethylene imine, thiol groups are modified on the other end of the linear polyethylene imine, and the gold nanoparticles are connected with the polyethylene imine through the thiol groups. The gold nanoparticles are prepared by reducing chloroauric acid with citric acid. The gold nanoparticles are introduced into the thiolated end group fluorinated linear polyethylene imine to synthesize a gene delivery carrier which has superior transfection performance and small cytotoxicity. The synthesis process is simple, has high repeatability, and is helpful to construct a high-efficiency low-toxicity cationic polymer gene delivery carrier.

Described herein are compositions and techniques related to generation and therapeutic application of artificial synapses. Artificial synapses are engineered extracellular vesicles, including exosomes, which incorporate sticky binders on their surface to anchor signaling domains against biological targets, such as receptors. These engineered additives can be organized in genetic vector constructs, expressed in mammalian cells, wherein the sticky binders attach to extracellular vesicles such as exosomes, thereby presenting their joined signaling domains which are rapidly taken up by recipient cells. Artificial synapses adopt the hallmark biophysical and biochemical features of extracellular vesicles, allowing for rapid deployment and scale-up. Importantly, this strategy can allow for kinetically favorable signal generation and signal propagation. This includes, for example, increasing density of agonist presentation to support receptor clustering—an onerous barrier for traditional receptor targeting strategies.

The present invention discloses a cationic lipid compound, its preparation method, and its application. The synthesis of the cationic lipid compound in the present invention is unrestricted in the choice of isonitrile and can be performed normally without the presence of a catalyst. The method for synthesizing the cationic lipid compound in the present invention is simple, inexpensive, and easily applicable. Furthermore, the synthesized lipid molecular structures are diverse, and the synthesis yield is high, exceeding 70%. Gene vectors prepared using the cationic lipid compound in the present invention exhibit good gene transfection efficiency and low cytotoxicity, and have potential for clinical application.

The invention provides a tumor-associated fibroblast targeting nano adjuvant as well as a preparation method and application thereof, and belongs to the field of nano drugs. The nano adjuvant (named as PF9PEI and alpha PC NPs) provided by the invention is coupled with PEI25K modified by 3-perfluorobutyl-1, 2-epoxypropane by utilizing a PD-L2 targeting peptide nrirgenyryh, so that an efficient gene vector (PF9PEI) is formed, and is used for loading an alpha PD-L1-P2A-CD86 plasmid. According to the nano adjuvant, CAFs can be reprogrammed into engineered antigen presentation CAFs by expressing a costimulatory molecule CD86, so that T cell immunity of breast cancer specificity is activated, and T cell failure is retarded at the same time. Besides, the engineered CAFs generated by the nano adjuvant generate alphaPD-L1 locally, so that the immune escape mechanism of breast cancer cells is effectively blocked, and the immune surveillance function of T cells is recovered. After radiotherapy, by using the nano adjuvant for treatment, the generation of central memory T cells is promoted, the progress of the same solid tumor is inhibited, the tumor metastasis is eliminated, and the tumor recurrence is inhibited.

The invention discloses a rhamnosyl transferase mutant as well as a coding gene, a carrier, a recombinant bacterium and application thereof, which are characterized in that rhamnosyl transferase from Fagopyrumesculentum is mutated, 17-site Tyr is mutated into Asp, 35-site Ser is mutated into Phe, 74-site Gly is mutated into His and 78-site Gly is mutated into Ala so as to construct a quadruple mutant M4, so that rutin can be catalytically synthesized by glycosylation by taking isoquercetin as a substrate, and the coding gene, the carrier, the recombinant bacterium and the application of the rhamnosyl transferase mutant are obtained. The rutin is expected to be synthesized by using E.coli, and theoretical and technical bases are provided for establishing an industrial rutin production process which is low in production cost, high in production intensity and small in environmental pollution.