59 results about "Gene vector" patented technology

The invention belongs to the field of genetic engineering and resource recycling, and relates to a ferrous oxide recombinant bacterium, a recombination method and application of the ferrous oxide recombinant bacterium in lithium battery metal recovery. The ferrous oxide recombinant bacterium is obtained by taking thiobacillus ferrooxidans as an original strain through genetic engineering modification; comprising thiobacillus ferrooxidans, an exogenously introduced nickel / cobalt efflux protein RcnA gene, a vector pJRD215 and donor bacteria, and the nickel / cobalt efflux protein RcnA gene is introduced into the vector pJRD215, driven to express and then introduced into the donor bacteria to express. The application of the constructed and prepared ferrous oxide recombinant bacteria in lithium battery metal recovery is used for improving the leaching efficiency of metal ions Co < + > or / and Li < + > or / and Ni < + > in lithium battery powder; and the method shows good adaptability and stability under different substrate concentrations, and provides a key experimental support and an important experimental basis for application of a microbial enhanced leaching technology in the field of waste lithium battery resource utilization.

The invention provides a territorial space planning data monitoring evaluation method and system, and relates to the technical field of territorial space planning. The territorial space planning data monitoring and evaluation method specifically comprises the following steps: S1, inputting multi-source territorial space data into a space DNA encoder, and generating three types of gene vectors, namely a topographic gene T-Gene, an ecological gene E-Gene and a construction gene U-Gene, by taking a 30m * 30m grid as a basic unit. Territorial space evolution is converted into a digital model, and a traditional planning problem is solved; the space DNA folding improves the early warning sensitivity; a self-repairing strategy is accurately released; perspective of conflict hotspots is realized through a three-dimensional dynamic instrument panel; autonomous iterative optimization of the system provides a scientific operable normal form for sustainable development of territorial space.

This invention relates to the field of genetic engineering, specifically to an HSL mutant, gene, vector, cell, composition, and its applications. The mutant is selected from... Os HSL4 enzyme, Sb HSL1 enzyme, Zm HSL1A enzyme, Hv HSL6D enzyme and Zm A mutant of at least one of the HSL1B enzymes. The HSL mutants, genes, vectors, cells, and compositions provided by this invention can improve plant resistance to HPPD inhibitors.

This invention discloses proteins related to the regulation of lily bulblet formation, their encoding genes, and their applications. The lily bulblet formation regulation proteins disclosed in this invention are either A1) or A2): A1) is a protein with the amino acid sequence of Sequence 2; A2) is a protein with one or more amino acid residues substituted and / or deleted and / or added to the amino acid sequence shown in Sequence 2 in the sequence listing, and has the same function as the protein shown in Sequence 2. This invention isolated and identified a LiARF6 gene related to bulblet formation from scale cuttings in 'Siberian' lily, and improved it using VIGS technology. By infecting bulbs with a bacterial solution containing this gene vector, the effect of enhancing the formation of bulblets during scale cutting was successfully achieved, providing a new method for promoting lily scale cutting propagation.

The invention discloses a redox sensitive cationic polymer gene vector as well as preparation and application thereof. According to the invention, hydrophobic small molecules with oxidation / reduction responsive S-S bonds are adopted to carry out side chain modification on polyglycidyl amine, and the cationic polymer gene delivery carrier material with specific tumor microenvironment response capability is prepared. The material has good biocompatibility, and can effectively load siRNA molecules and form siRNA compound nanoparticles with good stability and excellent gene transfection capacity. The compound nanoparticles can be effectively absorbed by cells, and can effectively respond to an oxidation / reduction tumor microenvironment to realize targeted release of siRNA. The oxidation / reduction response cationic polymer gene vector provided by the invention has a relatively great clinical application prospect.

This invention discloses a basic amino acid-modified 3S-PCL, its preparation method, and its applications, relating to the field of biomedical technology. The structural formula of the basic amino acid-modified 3S-PCL is as follows: [Structure formula would be inserted here]; where R is a basic amino acid; m, m1, and m2 are all positive integers, and m, m1, and m2 ≥ 1. This invention designs and synthesizes a basic amino acid-modified three-armed polycaprolactone (3S-PCL) material, which exhibits good biocompatibility and can replace cationic liposomes for nucleic acid delivery via lipid nanoparticles. The preparation method of this basic amino acid-modified 3S-PCL material is simple and rapid, particularly suitable for industrial production. Lipid nanoparticles prepared using this basic amino acid-modified 3S-PCL material as a gene carrier can achieve highly efficient delivery of nucleic acid drugs.

The invention discloses a PID effect accelerated aging prediction method for a component, and aims to solve the problems of individuation and mechanism uncertainty in component aging prediction. The method comprises the following steps: firstly, establishing a component gene vector containing multi-source heterogeneous attributes for each component so as to instantiate a digital twin; secondly, constructing a shared causal guided generative model taking a physical causal map as a structural constraint as a prediction core; in dynamic operation, the system performs probabilistic path prediction on a single twinborn body, and dynamically calibrates the state of the twinborn body by using new measured data through a data assimilation technology. Furthermore, the system inversely optimizes the physical causal atlas by using a structure learning algorithm by aggregating evolution data of the whole twinborn cluster, so as to realize iterative upgrade of the prediction core. According to the method, individual self-adaptive calibration and swarm intelligent evolution are combined, so that the system has the capability of discovering and perfecting physical laws from data, and the accuracy and robustness of long-term prediction are remarkably improved.

The application discloses a single-cell transcriptome data pseudo-time trajectory analysis method and a computer system, which comprises the following steps: 1) calculating a gene explicit comparison advantage matrix; 2) obtaining a gene similarity matrix by similarity and constructing a gene network; 3) taking an initial node in the gene network, starting random walking from the initial node, recording each gene walked through to form a gene text composed of gene sequences; 4) converting the gene text into a gene word vector; 5) adding all single-cell expressed gene vectors with expression as weight to form a sum vector as a word vector representation of the single cell in the gene space; and 6) visualizing all cell vector representations to obtain an embryo cell development pseudo-time trajectory result. The application provides an analysis basis for identifying different rare cell subtypes in tissues and variant genes of different cell subtypes and has a wide and important application prospect in the fields of tumors, developmental biology and life science.

The invention relates to the field of gene vector construction, in particular to construction and application of a gene vector integrating fluorescent screening and self-deletion functions, the gene vector comprises a conditional knock-out (cKO) vector and a gene knock-in (KI) vector which are both integrated with a Dre-Rox mediated self-deletion module and a fluorescent screening module, the construction method comprises the following steps: carrying out HindIII / EcoRI double enzyme digestion on a pUC19 vector, carrying out homologous recombination amplification on a target fragment, carrying out connection transformation and screening verification to obtain a qualified vector; the vector is applied to preparation of cKO / KI gene modified mice, and efficient screening of positive individuals is realized through fluorescence preliminary screening, genotype identification and fluorescence quenching detection. The positive screening workload and cost can be reduced by 70% or above, non-target elements are accurately cut off, interference is avoided, time-space accurate regulation and control are achieved, carrier construction is easy and convenient, repeatability is high, and the method is suitable for gene function research, disease model construction and drug target verification.

The invention discloses a regional scale city form multi-objective optimization method and system based on proxy model assistance, and the method comprises the steps: carrying out the discretization of a city region, obtaining a plurality of grid units, and obtaining a global gene vector and a local gene vector of each grid unit; decoding the gene vector to obtain an urban morphological feature matrix; obtaining the total energy demand of the region by using the proxy model; constructing a multi-objective optimization model by taking minimization of the total energy demand of the region and maximization of the development intensity as objective functions; on the basis of a multi-objective evolutionary algorithm, population individuals are converted into a feature matrix, the individual fitness is evaluated by utilizing an agent model, iterative search is performed on gene vectors, and a Pareto optimal solution set is obtained and serves as an urban form optimization scheme. According to the method, through an innovative hierarchical gene coding technology and an efficient proxy model evaluation technology, on the premise that the planning feasibility is guaranteed, the tradeoff relation between the regional scale urban form and the energy performance is rapidly, accurately and widely explored.

This invention relates to the field of gene vector technology, and more particularly to an ionizable comb-like polymer, its preparation method, and its applications. This comb-like polymer is a block polymer, comprising at least one block unit. Each block unit includes a first block, a second block, and a third block linked by covalent bonds. It exhibits high molecular flexibility and readily binds to nucleic acid-like substances to form polymer / nucleic acid complexes. Due to the presence of easily degradable intracellular glycosidic bonds in its structure, the polymer demonstrates high transfection efficiency. Furthermore, cell experiments show that the polymer / nucleic acid complex exhibits low toxicity to cells and significantly improves cell survival compared to the positive control. Therefore, it holds promise for widespread application in cell transfection. The preparation method provided by this invention utilizes a redox amination tandem reaction to directly prepare the target product, eliminating the need for intermediate product purification processes. This simple process facilitates large-scale production.

The present application relates to the technical field of recombinant protein technology, and particularly relates to a high-salt-tolerant amidase, gene, vector, recombinant bacteria and application, wherein the amino acid sequence of the amidase is shown as SEQ ID NO. 1. The amidase has extremely high salt tolerance and good ethanol tolerance. The recombinant amidase prepared by the present application lays a foundation for future realization of elimination of ethyl carbamate in high-salt fermented food and alcoholic beverages and realization of industrial application of ethyl carbamate hydrolytic enzyme, and has great economic and social benefits.

The invention provides application of a transcription factor OsNAC10 in improvement of rice phosphorus utilization and yield. The application comprises any one of the following applications of a rice OsNAC10 gene, the transcription factor OsNAC10 and a recombinant vector containing the OsNAC10 gene: A, improvement of rice phosphorus utilization; b, preparing a product for improving phosphorus utilization of rice; c, increasing the rice yield; and D, preparing a product for improving the rice yield, wherein the nucleotide sequence of the rice OsNAC10 gene is as shown in SEQ ID NO: 1. The invention provides a new theoretical basis and genetic resources for the cultivation of high-efficiency phosphorus varieties of rice.

The application provides sgRNA which specifically targets the Rosa26 safe site of a cashmere goat and application of using CRISPR / Cas9 to complete site-directed knockout and site-directed integration of an EGFP gene. The application firstly uses a bioinformatics method to predict the complete sequence of the Rosa26 site, then designs two sgRNA aiming at the site, constructs a targeting vector based on the CRISPR / Cas9 system, verifies the guiding efficiency of the sgRNA, simultaneously constructs a homologous integration EGFP gene vector, co-transfects the sgRNA and the homologous integration vector into cashmere goat fetal fibroblasts, and obtains a cell strain of the Rosa26 site site-directed integration of the EGFP gene. The efficiency of the sgRNA of the application in specifically guiding Cas9 to cut the Rosa26 site reaches about 40%, effectively reduces the off-target phenomenon existing in the CRISPR / Cas9 system, and further reduces the mutation of non-target gene sequences caused by non-specific cutting. The Cas9 / gRNA expression vector can realize the specific knockout or knock-in of the Rosa26 site at the cell, embryo or even individual level, so as to study the expression of specific genes and provide technical support for the cultivation of new goat breeds.

The application discloses a robot morphology directional evolution method based on environment perception and a related device, and relates to the technical field of robot morphology evolution. The method comprises the following steps: initializing a morphology gene vector and a control strategy of a robot; sampling and executing an action strategy according to the control strategy and environment perception information, and then calculating a system benefit value; judging whether the morphology gene vector of the robot is adapted to a current environment according to the environment perception information and the system benefit value; when the judgment result is yes, performing a mutation operation on the morphology gene vector of the robot based on a morphology mutation matrix; otherwise, jumping back to the step of sampling and executing the action strategy until a maximum iteration number is reached. The application quantifies the cumulative stimulation of environmental changes on the robot through an environment perception function to determine whether the robot needs to perform morphology evolution, and guides the direction of morphology evolution of the robot in different environments through the morphology mutation matrix, thereby accelerating the morphology evolution process of the robot.

This invention belongs to the field of biodetection technology, specifically relating to a material for detecting anti-NF155 antibodies and its preparation method. This invention constructs an expression plasmid capable of highly expressing the NF155 antigen, transfects it into HEK293T cells, and modifies the pCDH vector with enhancers during construction. Subsequently, a modified cell culture protocol is used to obtain a material for detecting anti-NF155 antibodies based on a cell immunofluorescence methodology. This invention uses a cell immunofluorescence methodology to maximize the preservation of the antigen's spatial conformation through cell expression and antigen immobilization, preventing false negatives due to changes in binding sites. This invention selects the lentiviral vector pCDH to avoid the problem of non-specific binding that is difficult to distinguish, as the proteins expressed by common target gene vectors such as pcDNA3.1 may themselves contain antibody binding sites. This improves detection specificity and solves the problem of false negatives.

Providing gene vector regulation using microRNAs expressed in cardiomyocytes. [Solution] This disclosure provides vectors and methods of using them for cell-type-specific repression of the expression of transgenes (e.g., cardiomyocyte reprogramming factors) using microRNA binding sites. The present invention generally relates to vectors and methods of using them for cell-type-specific repression of the expression of transgenes (e.g., cardiomyocyte reprogramming factors). This disclosure provides vectors comprising microRNA binding sites configured to promote specific repression of the expression of transgenes (e.g., cardiomyocyte reprogramming factors) in cardiomyocytes and cardiomyocyte progenitor cells compared to cardiac fibroblasts.

The present invention provides gene therapy plasmids and transgenes comprising a variant of ADAMTS13, recombinant virus vectors for delivery of said viral plasmids and transgenes and medical uses relating to the treatment of ADAMTS13 insufficiency.

Provided are a ketoreductase mutant, a coding gene, a vector, and use. The nucleotide sequence of the ketoreductase is set forth in SEQ ID NO: 2, and the mutant thereof is applied in the enzymatic preparation of eslicarbazepine, which can overcome the defects of poor tolerability and low activity of organic solvents, promoting the industrial application of enzymatic preparation of eslicarbazepine.

The application provides application of PavDREB1 gene in regulation of aging direction of sweet cherry fruits, and belongs to the technical field of molecular breeding.The nucleotide sequence of the PavDREB1 gene is shown as SEQ ID NO.1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO.2.The application also provides an overexpression vector and a VIGS gene silencing gene vector constructed from the gene and a construction method of the vector, and recombinant microorganisms containing the vector.The application finds that overexpression of the PavDREB1 gene can accelerate fruit aging and increase the expression level of aging-related genes by injecting the engineering bacteria containing the related vector into the equator of the fruits 25 days after flowering of the sweet cherry, and silencing the gene can slow down fruit aging and the expression of related genes.The application provides a new molecular assisted breeding strategy and theoretical basis for postharvest preservation of sweet cherry fruits.

The present application relates to the technical field of biological chemical industry, and particularly relates to an acid-responsive gene carrier, a construction method and application thereof.The present application provides acid-responsive supramolecular nanoparticles SNs based on star cationic polymer beta-CD-PDMAEMA and Pasp-benzoic imine-Ad host-guest interaction of adamantane modified polyaspartamide, and experimental results show that the mixing ratio (Ad / CD) of Pasp-benzoic imine-Ad and beta-CD-PDMAEMA, and the mixing ratio (N / P) of SNs and DNA can affect cell toxicity, transfection efficiency and phagocytosis efficiency, wherein the complex has high cell transfection endocytosis efficiency and stability when the N / P ratio is 20 and the Ad / CD is 2 or 4; based on this, the carrier can be used for expression, gene silencing and / or intracellular endocytosis of tobacco or other plant and animal genes, or for breeding improvement of tobacco and other plant and animal cells, or for gene therapy, and has a wide application prospect.

A “localizable” systemic gene therapy system is provided substantially increasing the transfection efficiency of the gene vectors into targeted tissue cells and substantially reducing the escape of the gene vectors from the targeted tissue volume, such as would waste the vectors, promote undesired immune reactions, and / or incur prohibitive costs for the required dose of gene-containing virus vectors. In this regard, the invention provides a means to simultaneously achieve local cell membrane permeability for virus vector transport and gene-containing vector injection in a portion of a vascularized organ. It includes a double-balloon catheter that create a finite contained volume in a blood vessel for the introduction of vectors with reduced loss along with a percutaneously inserted needle electrode providing increased cell membrane permeability for virus vector transport of the cells by creating an electric field in the same location where the vectors are injected.

The application provides a multifunctional shell material HITP and a preparation method and application thereof, and belongs to the field of biological medicines.The structure of the HITP is shown in formula I.The HITP can shield the positive charge of a gene carrier combined with the HITP, so that the gene carrier has a negative charge;the gene carrier with the negative charge can be used for in-vivo treatment through intravenous injection, and the problem of strong toxic side effects such as hemolysis caused by the combination of the existing cationic polymer gene carrier and negative proteins in blood is overcome;at the same time, the transfection efficiency of the cationic polymer gene carrier is not reduced after being combined with the shell material.In addition, the non-viral shell material HITP can enhance the immune activation effect.The shell material HITP has a good application prospect in the preparation of a gene carrier for in-vivo treatment.

The invention discloses an SNX9 engineered mitochondrial vesicle, a preparation method thereof and application of the SNX9 engineered mitochondrial vesicle in improvement of insulin resistance. The preparation method comprises the following steps: firstly, constructing a carrier for overexpressing an SNX9 gene, transfecting cells to obtain a cell strain for stably overexpressing the SNX9 gene, culturing, collecting a culture solution, and centrifugally separating to obtain SNX9-MDVs; and then, extracting mtDNA from the cells, and introducing the mtDNA into the SNX9-MDVs, so as to obtain the engineered mitochondrial vesicle mtDNA-SNX9-MDVs rich in the mtDNA. The mtDNA-SNX9-MDVs is used for treating insulin resistance, and the result shows that the mtDNA-SNX9-MDVs can effectively increase the content of ATP and mtDNA of mitochondria, enhance the activity of cell antioxidant enzyme, reduce ROS, improve the mitochondrial network structure and improve the expression of insulin signal channel related protein, and shows obvious mitochondrial function repair potential and insulin resistance improvement effect.

The present invention relates to branched poly(|3-amino ester) polymers (PBAE-Siaa) having the chemical structure of Formula X, multifunctionalized with silane and modified with amino acids, and the synthesis method of these polymers. A more effective gene delivery is achieved with the amino acid histidine, isoleucine, serine, methionine, phenylalanine or arginine and silane used during said modification.

The invention relates to the technical field of gene engineering, in particular to a forest tree exogenous gene integration method based on agrobacterium-mediated transient transformation. Connecting an exogenous gene with a vector to obtain an exogenous gene vector; transferring the exogenous gene vector into escherichia coli, extracting recombinant plasmids, and then transforming the recombinant plasmids into agrobacterium tumefaciens to obtain exogenous gene overexpressed agrobacterium tumefaciens; the exogenous gene overexpression agrobacterium infection plant is subjected to co-culture, and a co-cultured plant is obtained; treating the co-cultured plant into an explant, carrying out primary differentiation culture to obtain an adventitious bud, and carrying out strong seedling culture on the adventitious bud; and applying screening pressure to the strain with low overexpression multiple to perform secondary differentiation treatment so as to improve the expression quantity and the like. The invention not only has wide application prospect in genetic improvement of white birch, but also provides a new technical path for molecular breeding, gene function research and genome editing of other woody plants, and has remarkable scientific research value and economic application potential.

The present application relates to the technical field of artificial intelligence driven digital marketing, especially to an AI-based new media intelligent marketing method, comprising: step 1: cross-platform user behavior fusion modeling; step 2: multi-modal content gene library construction: analyzing the visual focus area and text emotional tendency of historical content, generating content gene vectors; step 3: dynamic content generation and real-time optimization: retrieving the gene library according to the space-time behavior matrix to generate initial marketing content; real-time monitoring of user interaction behavior, dynamically replacing the elements of the visual focus area and adjusting the emotional intensity of the text; step 4: closed-loop strategy optimization: calculating the dynamic effect index by fusing multi-platform indicators, triggering the gene mutation mechanism when the index continuously decreases, and generating cross-platform compensation content based on the failed user behavior. By analyzing the cross-platform behavior and content preferences of users, the user demand can be accurately predicted, and the content more in line with the individualized preferences of users can be provided.

The application discloses an esterase response cationic lipid molecule for gene delivery, wherein the cationic lipid molecule is designed with a large number of positive charges, can stably wrap nucleic acid drugs, and can remove the positive charges by the action of esterase after entering cells, so that charge reversal occurs, the system is neutral or negative, and the nucleic acid drugs can be quickly released for transfection. The nanoparticles formed by the cationic lipid have higher transfection efficiency than commercial liposomes, lower toxicity than commercial gene carriers, and have good application prospect.

The application is suitable for the technical field of data analysis, and provides a data analysis system and method for gene regulatory network based on deep regression algorithm, the method comprises the following steps: converting gene identity and gene expression value into vector representation; calculating attention score between gene vector representations as a judgment standard for the relationship between genes; and predicting specific gene expression value. The application enhances the anti-noise performance by introducing a Gaussian layer, captures the complex regulatory relationship between genes by using embedding and attention mechanism, and realizes individualized GRN prediction at the single cell level, which can overcome the defects of the existing method in the personalized treatment of cancer patients, and further support the gene expression analysis and prediction in the personalized treatment of cancer patients.

The invention provides a product for treating type 2 diabetes mellitus based on a stem cell insulin gene vector. The product consists of mesenchymal stem cells modified by genetic engineering and a cell preservation solution, the genetic engineering modified mesenchymal stem cells are obtained by transducing human mesenchymal stem cells by using a recombinant lentiviral vector, and the recombinant lentiviral vector can express a fusion protein composed of three parts of human proinsulin, a linker peptide and a GLP-1 receptor agonist. According to the application, human proinsulin and a GLP-1 receptor agonist are constructed into a single fusion protein, human mesenchymal stem cells are transduced by utilizing a lentiviral vector, and then a standardized three-stage induced differentiation process is carried out, so that a cell therapy product which can intelligently respond to blood glucose changes for a long time and has stable functions is finally obtained. The defects of transient treatment effect, single secretion function and large cell product batch-to-batch difference in the prior art are fundamentally overcome.