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Aminophenylboronic acid surface-modified nano-carbon material, as well as preparation method and application thereof

A nanocarbon material and surface modification technology, applied in the preparation method of peptides, nanotechnology for materials and surface science, nanocarbon, etc., can solve the problems of reduced possibility, signal inhibition, difficult analysis, etc. The effect of dispersibility and stability, strong specificity, good practical value and application prospect

Inactive Publication Date: 2013-04-17
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that most glycoproteins are low-abundance proteins, making them difficult to select for analysis by mass spectrometry
In addition, the ionization efficiency of non-glycoproteins / non-glycopeptides is much higher than that of glycoproteins / glycopeptides. Their presence in large quantities leads to severe inhibition of the signal of glycoproteins / glycopeptides in complex systems, greatly reducing the possibility of identification sex

Method used

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  • Aminophenylboronic acid surface-modified nano-carbon material, as well as preparation method and application thereof
  • Aminophenylboronic acid surface-modified nano-carbon material, as well as preparation method and application thereof
  • Aminophenylboronic acid surface-modified nano-carbon material, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Modification of aminophenylboronic acid on the surface of nanodiamond:

[0029] (1) Pretreatment of nano-diamonds: 1-5g nano-diamonds are ultrasonically dispersed in 10-50mL H 2 SO 4 / HNO 3 (9:1-8:2 (v / v)) solution, stir at room temperature for 12-24 hours, wash with water until neutral; as for 0.1-0.5M NaOH aqueous solution, stir at 90-95°C for 2-4 hours, wash with water Cleaning; Stir in 0.1-0.5M HCl solution at 90-95°C for 2-4 hours, wash with water, and dry to obtain acidified / activated nano-diamonds; the above treatment process makes the surface generate carboxyl functional groups.

[0030] (2) Disperse 0.5-1g acidified / activated nano-diamonds in 2-(N-morpholino)ethanesulfonic acid buffer (100-200mM, pH 6.1). Ultrasonic dispersion for 2-5 minutes, then quickly add 5-10mL 60-80mg / mL N-ethyldimethylaminopropylcarbodiimide hydrochloride, continue ultrasonic dispersion for 10-30min, after the reaction is over, collect nano-diamonds by centrifugation ,...

Embodiment 2

[0033] Example 2. Modification of aminophenylboronic acid on the surface of graphene:

[0034] (1) Pretreatment of graphene: 1-5g graphene is ultrasonically dispersed in 10-50mL H 2 SO 4 / HNO 3 (9:1-8:2 (v / v)) solution, stir at room temperature for 12-24 hours, wash with water until neutral; as for 0.1-0.5M NaOH aqueous solution, stir at 90-95°C for 2-4 hours, wash with water Cleaning; stir in 0.1-0.5M HCl solution at 90-95°C for 2-4 hours, wash with water, and dry to obtain graphene oxide; the above treatment process makes the surface produce carboxyl functional groups.

[0035] (2) Disperse 0.5-1g graphene oxide in 2-(N-morpholino)ethanesulfonic acid buffer (100-200mM, pH 6.1) dissolved in 5-10mL 60-80mg / mL N-hydroxysuccinimide ). Ultrasonic dispersion for 2-5 minutes, then quickly add 5-10mL 60-80mg / mL N-ethyldimethylaminopropylcarbodiimide hydrochloride, continue ultrasonic dispersion for 10-30min, after the reaction is completed, the activated graphene, discard the ...

Embodiment 3

[0038] Example 3. Modification of aminophenylboronic acid on the surface of carbon nanotubes:

[0039] (1) Pretreatment of carbon nanotubes: 1-5g carbon nanotubes are dispersed in 10-50mL H 2 SO 4 / HNO 3 (9:1-8:2 (v / v)) solution, stir at room temperature for 12-24 hours, wash with water until neutral; as for 0.1-0.5M NaOH aqueous solution, stir at 90-95°C for 2-4 hours, wash with water Cleaning; stir in 0.1-0.5M HCl solution at 90-95°C for 2-4 hours, wash with water, and dry to obtain oxidized / acidified carbon nanotubes; the above treatment process makes the surface produce carboxyl functional groups.

[0040] (2) Disperse 0.5-1g of oxidized / acidified carbon nanotubes in 2-(N-morpholino)ethanesulfonic acid buffer (100-200mM) dissolved in 5-10mL of 60-80mg / mL N-hydroxysuccinimide , pH 6.1). Ultrasonic dispersion for 2-5 minutes, then quickly add 5-10mL 60-80mg / mL N-ethyldimethylaminopropylcarbodiimide hydrochloride, continue ultrasonic dispersion for 10-30min, after the ...

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Abstract

The invention belongs to the technical field of inorganic materials and bioanalysis and in particular relates to an aminophenylboronic acid surface-modified nano-carbon material, as well as a preparation method and application thereof. According to the invention, an available nano-carbon material is subjected to carbodiimide reaction on the basis of acid treatment. Firstly, the surface of the nano-carbon material is modified by polylysine and polyethylene glycol / diglycolic acid, then the nano-carbon material reacts with aminophenylboronic acid for surface functional modification of the nano-carbon material. The synthesis method provided by the invention is simple and efficient. The prepared aminophenylboronic acid-functionalized nano-carbon material, as a nano-adsorbent, has a large specific surface area and strong selective specificity, exhibits better dispersibility and stability in aqueous solutions, and can rapidly achieve the effect of specifically separating and concentrating glycopeptide / glycoprotein. The material is significant in use value and application prospects in the glycoprotein proteomics field and the like.

Description

technical field [0001] The invention belongs to the technical field of inorganic materials and biological analysis, and in particular relates to a nano-carbon material surface-modified with aminophenylboronic acid and a preparation method and application thereof. Background technique [0002] The core of proteomics lies in the comprehensive analysis of proteins on a large scale, through the analysis of all protein properties of a species, individual, organ, tissue or cell (including expression level, structure, distribution, function, abundance change, post-translation Modification, intracellular localization, protein-protein interaction, protein-disease correlation) research, to make a detailed and accurate elaboration of protein function. Among them, the changes of post-translationally modified proteins often have important physiological functions, so the analysis and identification of post-translationally modified proteins is a hot issue in the field of biological analysi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B31/02B82Y30/00C07K1/14C12N9/08C01B32/15
Inventor 魏黎明徐国宾张伟陆豪杰杨芃原
Owner FUDAN UNIV
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