Avian infectious laryngotracheitis-fowl pox combined active vaccine

A chicken infectious and laryngotracheitis technology, applied in the field of veterinary vaccines, can solve the problems of immune chicken conjunctivitis, rising breeding costs, and affecting immune effects, etc., to reduce stress responses, facilitate storage and transportation, and simplify immunization operations Effect

Active Publication Date: 2013-07-03
PU LIKE BIO ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because of the different vaccination methods, these two diseases require at least two vaccinations to prevent them. Multiple vaccinations not only increase the cost of breeding, but also stimulate the chicken body many times, affecting the immune eff

Method used

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  • Avian infectious laryngotracheitis-fowl pox combined active vaccine
  • Avian infectious laryngotracheitis-fowl pox combined active vaccine
  • Avian infectious laryngotracheitis-fowl pox combined active vaccine

Examples

Experimental program
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Embodiment 1

[0028] 1. Preparation method of chicken whole embryo fibroblast monolayer

[0029] Select 10-day-old SPF chicken embryos (purchased from Beijing Merial Weitong Experimental Animal Technology Co., Ltd.), disinfect the eggshell surface with 0.1% bromogeramine and 2% iodine tincture, and aseptically pick out the chicken embryos and put them into a sterilization container , add maintenance solution and wash 3 times to thoroughly wash away blood and viscous metabolites. Use sterilized scissors to cut the embryo body into tissue pieces the size of rice grains, pour them into 500ml neutral glass bottles filled with sterilized glass beads, and add 0.25% trypsin digestion solution (1:4) depending on the number of tissue pieces . Then put it into a 37°C water bath for digestion for 25 minutes (during this period, gently shake it 3 times to make the trypsin fully contact with the tissue fragments), and stop the digestion when the tissue fragments are fluffy and fluffy. Pour out the try...

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Abstract

The avian infectious laryngotracheitis virus and fowl pox virus are cultured by using chicken whole embryo fibroblasts. Particularly, the avian infectious laryngotracheitis virus is successfully cultured by using the chicken whole embryo fibroblasts, the avian infectious laryngotracheitis virus and the fowl pox virus cultured by using the chicken whole embryo fibroblasts are obtained, and a combined freeze-drying active vaccine after a proper freeze-drying protectant is added. The combined freeze-drying active vaccine can be inoculated for immunization in a subalar and subcutaneous inoculation manner, so that side effects of the avian infectious laryngotracheitis virus can be alleviated, immune operations can be simplified, stress response can be reduced and production cost can be lowered. At the same time, the freeze-drying protectant prepared by a novel formula can effectively prolong storage time of the active vaccine and provide convenience for storing and transporting the products.

Description

technical field [0001] The invention relates to a chicken infectious laryngotracheitis and fowl pox dual live vaccine, in particular to a chicken infectious laryngotracheitis and fowl pox dual freeze-dried live vaccine, which belongs to the field of veterinary vaccines. Background technique [0002] Chicken infectious laryngotracheitis is an acute, contact upper respiratory infectious disease caused by infectious laryngotracheitis virus. It is characterized by dyspnea, coughing, and expectoration of an exudate containing blood samples. During autopsy, swelling, hemorrhage and erosion of the larynx and tracheal mucosa can be seen. In the early stage of the disease, the affected cells can form nuclear inclusion bodies. After the disease was first reported in the United States in 1925, it has now spread to many chicken raising areas in the world. The disease spreads quickly and has a high mortality rate. It occurs and prevails in many areas in my country, endangering the dev...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61P31/14A61P31/20A61K39/215A61K39/275
Inventor 张许科孙进忠
Owner PU LIKE BIO ENG
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