Test strip for detecting infectious laryngotracheitis virus, preparation method, detection method and kit

A laryngotracheitis virus and infectious technology, which is applied in the field of test strips for detecting infectious laryngotracheitis virus, can solve the problems of economic loss in the chicken industry, unfavorable rapid detection, difficulty in clinical diagnosis, etc., and achieves a wide range of detection samples. , The effect of fast detection speed and low professional level requirements

Inactive Publication Date: 2019-08-20
SICHUAN ANIMAL SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once the susceptible chickens are infected with the disease, it spreads rapidly, the infection rate reaches over 90%, and the fatality rate averages 10% to 20%.
The clinical symptoms and pathological changes of the disease are easy to be confused with diseases caused by other viruses, such as infectious bronchitis virus (IBV) and Newcastle disease virus (NDV). It brings certain difficulties, so it is usually necessary to use laboratory methods for diagnosis, and laboratory methods take a long time to confirm the diagnosis, which is not conducive to rapid detection in actual production

Method used

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  • Test strip for detecting infectious laryngotracheitis virus, preparation method, detection method and kit
  • Test strip for detecting infectious laryngotracheitis virus, preparation method, detection method and kit
  • Test strip for detecting infectious laryngotracheitis virus, preparation method, detection method and kit

Examples

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Embodiment 1

[0037] The invention provides a test strip for detecting infectious laryngotracheitis virus. refer to figure 1 As shown, including the bottom plate and the absorbent material bonded to the edge of the bottom plate. In this embodiment, the base plate is preferably a PVC rubber plate, and the surface of the base plate is sequentially laid with a sample pad, a colloidal gold bonding pad, and a nitrocellulose membrane with T lines and C lines in advance, and the nitrocellulose membrane The upper edge of the nitrocellulose membrane is bonded to the lower edge of the water-absorbing material, the lower edge of the nitrocellulose membrane is bonded to the upper edge of the colloidal gold bonding pad, the lower edge of the colloidal gold bonding pad is bonded to the upper edge of the sample pad, and the T line is wrapped have monoclonal antibodies to infectious laryngotracheitis virus. figure 1 The detection line T and the quality control line C in the figure represent the T line an...

Embodiment 2

[0064] This embodiment provides a method for judging the results of the infectious laryngotracheitis virus detection test strip.

[0065] Test strips were randomly selected to test the standard ILTV-negative samples (sample diluent) and the standard ILTV-positive samples, and the test results were referred to figure 2 shown. figure 2 The results showed that negative samples ( figure 2 b) There is only a rose red line on line C; while the standard ILTV-positive sample ( figure 2 a) There are two rose red lines, T line and C line.

[0066] The principle of judging the result of the infectious laryngotracheitis virus detection test strip provided by the present invention is: when the sample to be tested is added to the sample pad, the sample enters the colloidal gold binding pad under the action of chromatography, if the sample to be tested is infected with Infectious laryngotracheitis virus, the infectious laryngotracheitis virus in the sample to be tested is specifically...

Embodiment 3

[0070] This example provides a sensitivity test for the detection of ILTV. Use sample diluent 50ng / ml, 500ng / ml, 5000ng / ml instead of sample stock solution, use PBS liquid as negative control, carry out specificity determination to the test strip in embodiment 1. Measurement results refer to image 3 shown by image 3 c, 3d is available, the negative control and 50ng / ml sample dilution only appear a rose red line on line C, which is determined by image 3 a, 3b can be obtained, 500ng / ml, 5000ng / ml sample dilutions appear T line, C line 2 rose red lines. Therefore, according to the total protein concentration of the virus, it is judged that the detection sensitivity of the detection test strip provided by the present invention is 100 ng / ml.

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Abstract

The invention discloses a test strip for detecting an infectious laryngotracheitis virus, a preparation method, a detection method and a kit, and relates to the technical field of infectious laryngotracheitis virus detection. The test strip specifically comprises a bottom plate and a water absorption material bonded to the upper edge of the bottom plate. A sample pad, a colloidal gold bond pad anda nitrocellulose film are successively laid on the upper surface of the bottom plate along the chromatographic direction, wherein a T-line and a C-line are set on the nitrocellulose film in advance.The upper edge of the nitrocellulose film and the lower edge of the water absorption material are in lap joint. The lower edge of the nitrocellulose film is bonded to the upper edge of the colloidal gold bond pad. The lower edge of the colloidal gold bond pad is bonded to the upper edge of the sample pad. The T-line is coated with the monoclonal antibody of the infectious laryngotracheitis virus.The test strip for detecting the infectious laryngotracheitis virus has the advantages of high specificity, good repeatability and high sensitivity.

Description

technical field [0001] The invention relates to the technical field of infectious laryngotracheitis virus detection, in particular to a test strip for detecting infectious laryngotracheitis virus, a preparation method, a detection method and a kit. Background technique [0002] At present, avian infectious laryngotracheitis (AILT) is an acute, contact upper respiratory infectious disease caused by infectious laryngotracheitis virus (ILTV), which spreads rapidly. Since the disease was discovered in Los Angeles, USA in 1925, it has spread to chicken farming areas all over the world. The main lesions of the disease are in the larynx and trachea, and the clinical features are dyspnea, cough, and bloody exudate. Once the susceptible chickens are infected with the disease, it spreads rapidly, the infection rate reaches over 90%, and the case fatality rate averages 10% to 20%. The clinical symptoms and pathological changes of the disease are easy to be confused with diseases caus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/543
CPCG01N33/54306G01N33/56983
Inventor 康润敏谢晶于吉锋林毅曹冶李兴玉叶勇刚肖璐潘梦叶健强
Owner SICHUAN ANIMAL SCI ACAD
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