95 results about "Saccharomyces boulardii" patented technology

The invention discloses a microorganism preparation for improving cultivation water. The microorganism preparation is characterized in that raw materials include 20-40% of bacillus subtilis, 20-40% of bacillus amyloliquefaciens, 20-50% of saccharomyces boulardii and 5-10% of rhodopseudom onas palustrisand. A preparation method comprises the steps of: respectively inoculating the bacillus subtilis, the bacillus amyloliquefaciens, the saccharomyces boulardii and the rhodopseudom onas palustrisand into corresponding culture media to obtain seed culture solutions; inoculating the seed culture solutions into the culture media according to proportion in sequence for fermentation, and performing high-speed centrifugation to obtain high-concentration bacilli liquid; transferring the bacilli liquid into a matrix, mixing, granulating, drying and crushing. After the microorganism preparation disclosed by the invention is used, the cultivation water becomes clear, water change amount is reduced remarkably, water resources are saved, and pollution caused by outward emission is reduced. Due to improvement of the quality of a water environment, drugs used for aquaculture products are reduced, antibiotic pollution is reduced, the food safety requirement is met, and product quality is ensured.

An exemplary embodiment providing one or more improvements includes feeding animals with probiotic microbes encapsulated in a mixture of xanthan gum and chitosan, or in gelatin, specifically Pediococcus acidilactici and Saccharomyces boulardii. Such encapsulation protects the viability of the probiotic microbes against unfavorable temperatures. An exemplary embodiment providing one or more improvements includes methods of using viable probiotics in therapy of birds and mammals infected with infectious diseases. Probiotics acted as adjuvants in stimulating antibody reaction and stimulated a cellular immunity response. In particular, probiotics were shown to reduce the number of viable oocytes from fecal samples, stimulate antibody production, and stimulate of proliferation of splenocytes in chickens infected with Elimeria. In addition, probiotics were shown to relieve symptoms of parvovirus infection in dogs.

The invention discloses a breeding and production method of selenium-enriched yeast with high biomass. Through screening and domestication on a culture medium containing inorganic selenium, a strain of Saccharomyces cerevisiae subspecies Saccharomyces boulardii YBN-2 with high biomass and high content of selenium. Under optimal fermentation conditions, each gram of selenium-enriched yeast enriches as high as 2800-3200 mug / g of organic selenium, has viable count reaching 6.5-8.0*10<10> CFU / g. The selenium-enriched yeast provided by the invention has the advanategs of safety, no toxicity, simple production conditions, low cost and high economic value.

The invention discloses a complete fermented feed for a piglet creeping channel. The invention aims to fill the technical blank related to the complete fermented feed in the prior art. The complete fermented feed is formed by fermenting the fermenting base stock through a compound viable bacteria preparation. The fermenting base stock is prepared from the following raw materials by weight percent: 4-6% of fermented soybean meal, 8-12% of extruded soybean, 10-14% of bean pulp, 1-3% of plasma protein, 2-4% of soya-bean oil, 30-36% of corn, 18-22% of extruded corn, 8-12% of whey powder and 4-6% of premixed material. The compound viable bacteria preparation is composed of lactobacillus plantarum, pediococcus pentosaceus PP, bacillus subtilis, saccharomyces boulardii, beer yeast, candida utilis and clostridium butyricum. The complete fermented feed provided by the invention is fragrant and is capable of obviously promoting the feed palatability, bringing forward the mouth opening period of the piglet, obviously improving palatability, boosting ingestion and increasing feed intake, obviously increasing the digestive absorption utilization rate of the feed, optimizing intestinal flora, preventing and treating piglet scours and effectively reducing the diarrhea rate.

The invention discloses a compound probiotic peptide as well as a preparation method and an application thereof. The compound probiotic peptide is obtained by adding a bacterium enzyme compounding fermentation agent into soya bean meal and fermenting, wherein the bacterium enzyme compounding fermentation agent is formed by mixing four types of probiotics, four types of proteases and a carbon source; the four types of probiotics comprise clostridium butyricum, bacillus pumilus S3, lactobacillus plantarum and saccharomyces boulardii; and the four types of proteases comprise an acidic protease, a neutral protease, an alkaline protease and a polypeptide protease. The invention provides a specific preparation method of the compound probiotic peptide and an application of the compound probiotic peptide to preparing a piglet feed additive. The compound probiotic peptide product is enriched with a plurality of types of probiotics and antigen proteins can be effectively degraded to generate micro-molecular substances, namely polypeptides, amino acids and the like; and in a fermentation process, a plurality of types of flavor and antagonistic substances, namely probiotic organic acids, biotins and the like are generated, so that the palatability and the safety are increased, the utilization rate of the soya bean meal and the nutritive value of a feed are remarkably improved, and the diarrhea rate of piglets can be remarkably reduced.

The present invention includes a novel yeast strain of the genus Saccharomyces boulardii sequela PY31 ATCC 74366 that is able to process certain metallic compounds into biologically active forms suitable for supplementing the human diet. The present invention also includes methods for isolating such yeast, nutritional supplement compositions containing such yeast, and methods of administering the nutritional supplement compositions to humans.

The invention discloses an active dry saccharomyces boulardii feed additive and its preparation method and use. The preparation method of the active dry saccharomyces boulardii feed additive comprises the following steps of carrying out activation cultivation, enlarging cultivation and fermentation cultivation of a saccharomyces boulardii strain to obtain fresh yeast, and carrying out low temperature drying of the fresh yeast to obtain the active dry saccharomyces boulardii feed additive having water content less than or equal to 6 wt% and the viable count equal to or greater than 40000 million per gram. An addition amount of the active dry saccharomyces boulardii feed additive in chicken feed is in a range of 100 to 300 grams per ton and an addition amount of the active dry saccharomycesboulardii feed additive in pig feed is in a range of 300 to 1000 grams per ton. The active dry saccharomyces boulardii feed additive has natural antibiotic resistance thus overcoming defects of composite probiotics and feed yeast in application. The active dry saccharomyces boulardii feed additive can adjust microecological balance of an intestinal tract, improve livestock and poultry body immunity and absorption and digestion of feed nutrients, promote livestock and poultry growth and reduce a breeding cost.

The invention discloses a preparation method of a composite probiotic agent for preventing and treating diabetes and controlling body weight, in particular to a composite probiotic agent formed by compounding probiotics, prebiotics and metformin and used for preventing and treating diabetes and controlling body weight and a preparation method of the composite probiotic agent. Beneficial bacteria such as clostridium butyricum, saccharomyces boulardii, lactobacillus reuteri, bifidobacterium infantis, bacillus licheniformis and the like, the prebiotics such as arabinose and xylooligosaccharide, and metformin are combined to avoid symptoms such as nausea, emesis, diarrhea, abdominal pain, constipation, abdominal distention, dyspepsia, heartburn and the irritable bowel syndrome which are caused by the metformin, the curative effect is good, glucose metabolism is accelerated by directly absorbing and utilizing glucose, and effects of controlling body weight and treating the diabetes are improved. In the aspect of improving adverse effects of the metformin drug to a gastrointestinal tract, the effect of the composite probiotic agent is higher than that of the metformin which is applied purely.

The invention relates to a preparation method of a high-density fermented and freeze-dried active microbial agent of a forage anti-diarrhea yeast, and belongs to the field of an agriculture biotechnology. The method comprises the following steps: inoculating an original starting strain (Saccharomyces boulardii, ATCC MYA-796) into yeast leaching peptone powder glucose liquid culture medium after activating; cultivating at 30 DEG C for 12-16 hours at 200-250rpm as a seed solution; inoculating the seed solution by 10% of inoculation amount, cultivating at 30 DEG C for 12 hours at 800rpm in liquid-expanded yeast leaching peptone powder glucose liquid culture medium, and then feeding 25% glucose for 6 hours, wherein the feeding amount is 20ml/h/L of fermentation liquor; centrifugally collecting thalli and adding protective agents (8% of glycerinum, 7.5% of skim milk powder, 7.5% of mycose and 0.9% of sodium chloride), wherein the mixing ratio of the protective agents to the thalli is 2:1; stewing and balancing for an hour after evenly mixing; pre-cooling at -80 DEG C for 12 hours, and then freeze-drying the thalli in a vacuum freezer dryer; weighing freeze-dried powder to count viable bacteria, wherein the viable bacteria count achieves 1.4*10<10> CFU/g.

The invention discloses high-temperature and high-humidity resistant saccharomyces boulardii and application thereof, and belongs to the field of microorganisms. The preservation number of the high-temperature and high-humidity resistant saccharomyces boulardii disclosed by the invention is CGMCC No.10381; the multiplication rate and maximal biomass (namely final density of thallus) of saccharomyces boulardii strains are significantly higher than those of similar saccharomyces cerevisiae strains; the saccharomyces boulardii has high heat-resistant and moisture-resistant capacities; the saccharomyces boulardii disclosed by the invention can be used as a feed additive; and relatively high activity is stored in the high-temperature and high-humidity treatment processes. According to a saccharomyces boulardii capsule, daily gain, immune organ index, disease resistance, oxidation resistance and immune globulin of broiler chicken can be improved; the saccharomyces boulardii is free of a toxic or side effect on beasts and birds, wide in source and low in price, and can be used as a feed additive instead of antibiotics.

The present invention discloses a probiotic composition for enhancing immunity and an application of the probiotic composition. The probiotic composition comprises prebiotics, a K-MYL probiotic combination and functional substances, and the K-MYL probiotic combination comprises at least one of lactobacillus casei, lactobacillus bulgaricus, saccharomyces boulardii, lactobacillus reuteri and bifidobacterium animalis, or further comprises at least one of lactobacillus rhamnosus, lactobacillus acidophilus and bifidobacterium longum. The probiotic composition can significantly increase the number of intestinal effective microbial communities, improve intestinal immune functions, improve expressions of intestinal immune factors, resist virus infection and shorten recovery time after the virus infection. Moreover, probiotic strain combinations, the prebiotics, probiotic strains and the functional substances have remarkable synergistic enhancement effects.

The invention provides a composite water quality improvement agent for aquaculture and a use method of the improvement agent, and relates to the field of water quality improvement. The improvement agent comprises the following raw materials in parts by weight: 13-18 parts of photosynthetic bacterium, 5-10 parts of nitrifying bacterium, 40-60 parts of oyster shell powder, 10-15 parts of composite polypeptide amino acid, 14-18 parts of humic acid, 10-20 parts of composite biological enzyme, 4-10 parts of bacillus subtilis, 8-15 parts of mannitol, 10-20 parts of enterococcus faecalis, 20-40 parts of activated carbon, 15-20 parts of monopotassium phosphate, 20-30 parts of potassium peroxymonosulfate composite salt, 3-7 parts of sodium lignosulphonate, 6-12 parts of saccharomyces boulardii, 10-15 parts of nano improved aluminosilicate, 3-7 parts of clostridium butyricum and 5-10 parts of vitamin. The improvement agent has the advantages of high efficiency, environmental protection and convenience in use, can effectively eliminate pool bottom effluvium, degrades and purifies organic waste and toxin, improves the aquaculture water quality, and improves the quality of an aquatic product.

The invention discloses a composite probiotic liquid for preventing diabetes and controlling body weight and a preparation method thereof. The composite probiotic liquid contains saccharomyces boulardii, acetobacter xylinum, lactobacillus plantarum, bifidobacterium adolescentis, bacillus licheniformis, clostridium butyricum, arabinose, xylooligosaccharide and bacterial cellulose and the like. After probiotics and prebiotics of the composite probiotic liquid enter the human body, the absorption of glycogens such as glucose by the intestinal tract can be quickly reduced, and thus the body weight is controlled and blood glucose is reduced. Clinical test results indicate that for a patient who orally administers the composite probiotic liquid, it is determined preliminarily that the composite probiotic liquid is more excellent than pure application of metformin in the aspects of time for blood glucose to meet the standard, sugar tolerance improvement, insulin improvement and c-peptide release, and has a remarkable weight loss effect. In addition, the composite probiotic liquid indeed has curative effects in the aspects of functions of improving insulin resistance and protecting islet cells and has no side effect.

The invention provides boulardii active dry yeasts, wherein the total amount of cells of the dry yeasts is 30-75 billions/g, the rate of living cells is 50-88%, and the water content is 4-6%. A production method of the boulardii active dry yeasts comprises the following steps of: (1) culturing strains: inoculating and culturing Saccharomyces boulardii strains; (2) culturing seeds in a fermentation tank; (3) ventilating air flows into the fermentation tank for culture, after 20-28 hours of fermentation, adjusting the pH value of fermentation liquor to be 6.1-6.4, performing cell decolorization to ensure that the color of the product is lighter, and in 1.5-3 hours after the pH value is adjusted to be 6.1-6.4, raising the temperature of the fermentation liquor to 30-35 DEG C to perform fermentation, and adding sodium chloride at the same time; (4) separating; (5) adding an emulsifying agent and vegetable oil, and then granulating and molding; and (6) drying to obtain yeast particles.

The invention discloses a preparation method and application of a fermented feed additive and belongs to the field of animal feed. The additive is prepared by a liquid-solid two-step fermentation method. The liquid-solid two-step fermentation method comprises the steps: firstly, performing liquid fermentation on probiotics of bacillus subtilis, lactobacillus acidophilus, clostridium butyricum, saccharomyces cerevisiae and saccharomyces boulardii; performing solid mixed fermentation for 5 to 7 days with solid raw materials after fermentation is finished; drying and packaging; then compounding acompound premix and compound enzyme to obtain a finished product. According to the fermented feed additive, corn bran, corn steep liquor, corn starch candy pomace, corn protein powder and the like are utilized as main raw materials; thus, the fermented feed additive has comprehensive nutrition, and a lot of active matters can be further generated after microbial fermentation. When the fermented feed additive is applied to laying hen breeding, organism immunity of the laying hens is improved, and disease resistance of the laying hens is enhanced; furthermore, the laying rate and egg quality ofthe laying hens can be improved. The raw materials of the fermented feed additive has wide source and is low in cost, no three wastes is generated in a production process, and environmental friendliness and safety are achieved. Thus, the fermented feed additive disclosed by the invention has better market application value.

The invention provides a preparation method of an antioxidant probiotic-containing milk drink. High fructose corn syrup, lactobacillus casei or lactobacillus paracasei freeze-drying powder, saccharomyces boulardii freeze-drying powder, puncturevine caltrop fruit polyaccharide and burdock polyaccharide, xylitol, erythritol and the like are added to skim milk; lactobacillus casei or lactobacillus paracasei in the antioxidant probiotic-containing milk drink can balance intestinal flora; saccharomyces boulardii can prevent and treat diarrhea; puncturevine caltrop fruit polyaccharide and burdock polyaccharide contributes to proliferation of probiotics in intestines and serve as stabilizers of the milk drink; xylitol and erythritol are both functional sugar alcohol and have the characteristics of low sweetness, low calorie, caries resistance, little influence on blood sugar and the like; accordingly, the antioxidant probiotic-containing milk drink can serve as caries prevention food, patients suffering from diabetes, hyperlipidemia or obesity can also take the drink, and the drink is suitable for a wide range of consumers and has multiple health care functions; besides, the survival rate of lactobacillus casei or lactobacillus paracasei can also be increased due to addition of saccharomyces boulardii.

The invention relates to synbiotic microcapsules and a preparing method and application thereof. A wall material of the synbiotic microcapsules is prepared from liquid paraffin and Span 80, and a capsule material is prepared from soybean peptide and saccharomyces boulardii. The preparing method comprises the steps that firstly, a capsule material solution and an oil phase are prepared, then the capsule material solution and the oil phase are mixed to be prepared into synbiotic microcapsules to be cultured, and finally the end products are prepared based on a fermentation culture method. The synbiotic microcapsules and the preparing method and application thereof have the advantages that according to the synbiotic microcapsules, soybean peptide and saccharomyces boulardii are wrapped into the same microcapsule, the growth promoting effect of soybean peptide on probiotics is performed, and the activity protection effect of the microcapsules on microorganisms is also performed; the end products can perform the health function of prebiotis, and the probiotic function of probiotics; the technology of carrying out wrapping before fermentation is adopted, saccharomyces boulardii obtains the opportunity for proliferation again, and thus the synbiotic microcapsules with higher density and activity can be easily obtained.

The invention discloses a traditional Chinese medicinal microecologics for preventing porcine respiratory diseases. The traditional Chinese medicinal microecologics comprises the following components (in parts by weight): 15-30 parts of tenuifolia, 10-25 parts of coptis chinensis, 10-28 parts of golden cypress, 10-25 parts of herba andrographitis, 10-25 parts of radix sophorae flavescentis, 15-30 parts of radices sileris, 20-30 parts of radix bupleuri, 10-25 parts of astragalus membranaceus, 10-30 parts of folium eriobotryae, 8-30 parts of mint and 5-20 parts of liquorice and also comprises 0.1-1.0*10<8>CFU/ml of lactobacillus, 0.1-1.0*10<8>CFU/ml of bifidobacterium, 0.1-1.0*10<8>CFU/ml of bacillus subtilis and 0.1-1.0*10<8>CFU/ml of saccharomyces boulardii. The invention also discloses a preparation method for the traditional Chinese medicinal microecologics for preventing the porcine respiratory diseases. The traditional Chinese medicinal microecologics for preventing the porcine respiratory diseases, which is prepared by the method disclosed by the invention, can effectively prevent and treat the porcine respiratory diseases.

The invention discloses a method for producing probiotic protein feed through peltate yam rhizomes. The method comprises the following steps that (1) peltate yam rhizome cellulose and starch are separated; (2) the peltate yam rhizome cellulose is fermented; (3) the peltate yam rhizome starch is liquefied and saccharified; (4) the probiotic protein feed is prepared. Firstly, the starch and the cellulose in the peltate yam rhizomes are separated through a mechanical method, the cellulose is used for solid state fermentation to obtain fibrous residues containing cellulose and mycoprotein, then a suspension solution for separation of the starch is used for subsequent extraction of saponin, supernate is added to the peltate yam rhizome starch, and circulation utilization of waste water is achieved. Peltate yam rhizome starch milk is used for cultivating probiotics after being liquefied and saccharified, the probiotics include saccharomyces boulardii and lactobacillus acidophilus, then cellulose fermentation broth and probiotic culture are completely mixed to be uniform, and the probiotic protein feed containing the cellulose is obtained. Comprehensive utilization of the starch and the cellulose in the production process of the diosgenin saponin is completely achieved, production benefits are improved, and exhaust amount and pollution are reduced largely.

The invention discloses a composite microorganism beverage and a preparation method thereof, and particularly discloses a composite microorganism beverage for treating high blood pressure and coronary heart disease and concurrently preventing and treating constipation and diarrhoea, and a preparation method of the composite microorganism beverage. The composite microorganism beverage is prepared from the following raw materials of streptomyces ogaensis fermentation liquor, clostridium butyricum fermentation liquor, saccharomyces boulardii fermentation liquor, bacillus laevolacticus fermentation liquor, lactobacillus brevis fermentation liquor, soybean flour, brown sugar, L-theanine, xylooligosaccharide and purified water. The microorganism beverage disclosed by the invention integrates treatment health-care functions of various single products, not only contains microorganism groups and metabolism products for preventing and treating the coronary heart disease and the high blood pressure, but also has microorganism groups and metabolism products for regulating the internal balance of the stomach and the intestine, and preventing and treating constipation and diarrhoea, integrates health-care and treatment functions, conforms to the current medicine viewpoint of people for combining prevention, health care, treatment and recovery, and is quite wide in market prospects.