Selenium-enriched yeast with high biomass, and additive and premix containing the same

A selenium-enriched yeast, high-biomass technology, applied in microorganism-based methods, microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of difficult absorption, inorganic selenium toxicity, unsuitability for humans and animals, etc. To achieve the effect of simple production method, easy promotion, and easy production and application

Active Publication Date: 2014-03-26
北京乾胜生物技术有限公司 +1
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  • Abstract
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  • Application Information

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Problems solved by technology

However, inorganic selenium has high toxicity and is not eas

Method used

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  • Selenium-enriched yeast with high biomass, and additive and premix containing the same
  • Selenium-enriched yeast with high biomass, and additive and premix containing the same
  • Selenium-enriched yeast with high biomass, and additive and premix containing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 Low-concentration sodium selenite plate screening

[0029] In the present invention, the determination of the tolerance of yeast to sodium selenite is used as a screening method, and the tolerance (50, 100, 300, 500 μg / mL) of yeast strains preserved in our laboratory to selenium is studied. The results showed that the tolerance of different yeast strains to sodium selenite was quite different. Use a sterilized toothpick to spot candidate yeast strains on the inorganic selenium plates containing 50, 100, 300, and 500 μg / mL respectively, and after standing at 30°C for 48 hours, select the yeast strains that grow better on the plates containing inorganic selenium. Good 8 strains (Saccharomyces cerevisiae S4, Saccharomyces cerevisiae S3, Saccharomyces cerevisiae S2, Saccharomyces boulardii YBN-3, Saccharomyces boulardii YBN-2, Saccharomyces boulardii YBN-1, Candida alkanogenes C, and Lasseria B) for further domestication studies ( figure 1 ).

Embodiment 2

[0030] Example 2 High-concentration sodium selenite plate acclimatization

[0031] The 8 screened strains were further acclimatized on high-concentration sodium selenite (1000, 2000, 3000 μg / mL) plates. On the plate containing 1000, 2000, 3000 μg / mL sodium selenite respectively, spot the yeasts screened in Example 1 with a sterilized toothpick. Saccharomyces cerevisiae boulardii YBN-2, which grew well on the sodium selenite plate, was used as a production strain for the next experiment ( figure 2 ).

[0032] The selected Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) subspecies of Saccharomyces boulardii named YBN-2, which was preserved on November 8, 2013 in the General Microbiology Center of China Committee for the Collection of Microbial Cultures, referred to as CGMCC, address: No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing Institute of Microbiology, Chinese Academy of Sciences, the deposit number is CGMCC No.8447.

Embodiment 3

[0033] Embodiment 3 Selenium-enriched yeast fermentation condition optimization

[0034] 1. The influence of different culture media

[0035] Saccharomyces boulardii strains were fermented and cultured with YPD and molasses medium respectively, the amount of inorganic selenium added was 30 μg / mL, and the liquid volume was 50 and 100 mL. The results are shown in Table 1. Different media had certain effects on the biomass and selenium content of Saccharomyces cerevisiae boulardii. It can be seen from Table 1 that the biomass and organic selenium content of the strains cultured in 50 and 100mL molasses fermentation medium were higher than those in YEPD medium. And compared with the YEPD medium, the molasses medium is easy to preserve, low in cost, easy to use, and suitable for industrial production. Therefore, molasses was selected as the carbon source for the next step of fermentation condition optimization.

[0036] Table 1 Effects of different fermentation media on the bioma...

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Abstract

The invention discloses a breeding and production method of selenium-enriched yeast with high biomass. Through screening and domestication on a culture medium containing inorganic selenium, a strain of Saccharomyces cerevisiae subspecies Saccharomyces boulardii YBN-2 with high biomass and high content of selenium. Under optimal fermentation conditions, each gram of selenium-enriched yeast enriches as high as 2800-3200 mug / g of organic selenium, has viable count reaching 6.5-8.0*10<10> CFU / g. The selenium-enriched yeast provided by the invention has the advanategs of safety, no toxicity, simple production conditions, low cost and high economic value.

Description

technical field [0001] The invention relates to the technical field of biological fermentation industry, in particular to a selenium-enriched yeast containing high biomass, a production method thereof, an additive and a premix containing the same. Background technique [0002] It plays a role in balancing the oxidation-reduction atmosphere, enhancing immunity, and playing a role in the prevention and treatment of diabetes, cataracts, cardiovascular and cerebrovascular diseases, Keshan disease, Kashin-Beck disease, arthritis and rheumatoid arthritis, and prevention of animal white muscle disease. play an important role. At present, the adult intake recommended by the Chinese Nutrition Society is 50-250 micrograms per day, while the selenium intake in two-thirds of my country is lower than the minimum recommended value. Selenium deficiency has also become a worldwide animal husbandry problem. So far, it has been found that about 40 species of animals have selenium deficiency ...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12Q1/02A23L1/30A23K1/16C12R1/865
Inventor 彭子欣吴栋冯秋月董晓丽王安如
Owner 北京乾胜生物技术有限公司
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