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High-temperature and high-humidity resistant saccharomyces boulardii and application thereof

A brady yeast, high temperature-resistant technology, applied in the field of microorganisms, can solve problems such as activity reduction, and achieve the effects of preventing bacterial inactivation, facilitating transportation, and preventing gastric juice from being damaged.

Active Publication Date: 2015-07-15
赤峰四海同心生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For this reason, the present invention aims at the problem in the prior art that the activity of Saccharomyces bladia is reduced during high-temperature and high-humidity treatment when it is added to feed, and provides a high-temperature and high-humidity saccharomyces bladia

Method used

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  • High-temperature and high-humidity resistant saccharomyces boulardii and application thereof
  • High-temperature and high-humidity resistant saccharomyces boulardii and application thereof
  • High-temperature and high-humidity resistant saccharomyces boulardii and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Microencapsulated Saccharomyces boulardii and its growth curve

[0032] 1. Method:

[0033] 1. Growth activity comparison:

[0034] Saccharomyces cerevisiae of the present invention is a Saccharomyces cerevisiae, and its growth activity is compared with that of common Saccharomyces cerevisiae (Saccharomyces cerevisiae, purchased from ATCC).

[0035]The method is as follows: activate the tested strains for two generations with an inoculum of 1% (about 2 × 10 6 cfu / mL) was inoculated into a 300 mL Erlenmeyer flask containing 100 mL of LYPD medium, cultured at 28° C., 180 rpm, sampling every 2 hours, and the absorbance at 600 nm was measured. Taking the culture time as the abscissa and the corresponding absorbance value as the ordinate, draw the growth curve.

[0036] In order to improve the tolerance to the gastrointestinal environment of Saccharomyces boulardii of the present application, Saccharomyces boulardii was microencapsulated to prepare Saccharomyce...

Embodiment 2

[0044] Example 2 In vitro evaluation experiment

[0045] 1. Method

[0046] 1) Microencapsulated Brady yeast and its culturing method are the same as above.

[0047] 2) In vitro evaluation experiments

[0048] ①The high temperature tolerance of microencapsulated yeast Brady was evaluated in a constant temperature drying oven. 1 g of microencapsulated yeast brady was treated at 110°C and 130°C for 30s, 45s and 60s, respectively. Radix yeast powder served as a control. In addition, the microencapsulated S. bradii and S. bradii powder were treated at 100% humidity, 75°C and 85°C for 1 min. The microencapsulated S. bradii needs to be broken and then counted by gradient dilution plate pouring method. Saccharomyces cerevisiae and Enterococcus faecium were cultured on YPD and MRS agar medium for 48h, and the number of viable bacteria was counted. Three repetitions.

[0049] ②Simulated gastric juice tolerance test. Transfer 0.5g of microencapsulated Saccharomyces boulardii and Sa...

Embodiment 3

[0056] Example 3: Breeding test of broilers

[0057] 1. Method:

[0058] Experimental animals: 400 1-day-old AA broiler chicks were randomly selected and randomly divided into 4 treatment groups with 5 replicates in each group and 20 chickens in each replicate. The experimental diets were: A. blank control group (basic diet group); B. basal diet + 1 g antibiotic (Aureomycin) / kg diet; C basal diet + 108cfu microencapsulated Saccharomyces boulardii / kg ration. The experimental period is 42d, 1-21d is the early stage of the test, and 22-42d is the late stage of the test. The basic diet is corn-soybean meal type, which is formulated in two stages according to the Chinese Broiler Breeding Standard (2004).

[0059] growth performance

[0060] On the 1st day, 21st day and 42nd day of the test, the rats were weighed on an empty stomach with each repetition as the unit. Repeated average daily gain (ADG), average daily feed intake (ADFI) and feed to weight ratio (F / G).

[0061] immu...

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Abstract

The invention discloses high-temperature and high-humidity resistant saccharomyces boulardii and application thereof, and belongs to the field of microorganisms. The preservation number of the high-temperature and high-humidity resistant saccharomyces boulardii disclosed by the invention is CGMCC No.10381; the multiplication rate and maximal biomass (namely final density of thallus) of saccharomyces boulardii strains are significantly higher than those of similar saccharomyces cerevisiae strains; the saccharomyces boulardii has high heat-resistant and moisture-resistant capacities; the saccharomyces boulardii disclosed by the invention can be used as a feed additive; and relatively high activity is stored in the high-temperature and high-humidity treatment processes. According to a saccharomyces boulardii capsule, daily gain, immune organ index, disease resistance, oxidation resistance and immune globulin of broiler chicken can be improved; the saccharomyces boulardii is free of a toxic or side effect on beasts and birds, wide in source and low in price, and can be used as a feed additive instead of antibiotics.

Description

technical field [0001] The invention relates to a high-temperature and high-humidity resistant yeast Brady and its application, belonging to the field of microorganisms. Background technique [0002] Saccharomyces boulardii, also known as Saccharomyces boulardii, belongs to a subspecies of Saccharomyces cerevisiae. Saccharomyces boulardii can effectively treat various types of diarrhea in humans and animals, and no adverse reactions have been found. After entering the intestinal tract of humans and animals, yeast Brady can not only degrade toxins produced by pathogenic bacteria and neutralize bacterial toxins, but also stimulate the intestinal mucosa to enhance immune function. Invasion of pathogenic bacteria, and Brady yeast preparation is a natural microecological preparation, which not only has no toxic and side effects, but also can provide certain nutrients. Therefore, the application of Brady yeast in animal husbandry is more and more extensive. [0003] As the only...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N11/10C12N11/04A23K1/17A23K1/18A61K36/064A61P31/00C12R1/85A23K20/195
CPCA61K36/064C12N11/04C12N11/10C12N1/185C12R2001/85
Inventor 魏永刚
Owner 赤峰四海同心生物科技有限公司
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