Microorganism preparation for improving cultivation water and preparation method thereof

A technology of microbial preparations and aquaculture water, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems that aquatic animals have no direct effect, achieve self-healing ability, reduce mortality, and reduce antibiotics The effect of using

Active Publication Date: 2012-10-03
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most microbial products only play a role in aquaculture wat

Method used

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  • Microorganism preparation for improving cultivation water and preparation method thereof
  • Microorganism preparation for improving cultivation water and preparation method thereof
  • Microorganism preparation for improving cultivation water and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The first step: Bacteria agent directional enrichment culture

[0041] a. Bacillus subtilis and Bacillus amyloliquefaciens were respectively inoculated in the first culture medium under aseptic conditions, and the content of each component in 1.0L distilled water in the first culture medium was: peptone 10.0g, beef extract 3.0 g, 5.0 g of sodium chloride, the pH value was adjusted to 7.0, and the single strain was cultivated for 48 hours at 35° C. to obtain the Bacillus subtilis seed culture solution and the Bacillus amyloliquefaciens seed culture solution respectively, and the number of viable bacteria was 6.0×10 cfu / ml;

[0042] b. Inoculate Rhodopseudomonas palustris into the liquid medium under aseptic conditions, and the content of each component in 1.0L distilled water in the second medium is: sodium bicarbonate 2.0g, sodium acetate 2.0g , ammonium chloride 1.0g, dipotassium hydrogen phosphate 0.5g, sodium chloride 12.0g, magnesium chloride 0.2g, yeast extract 0.5...

Embodiment 2

[0050] The first step: Bacteria agent directional enrichment culture

[0051] a. Bacillus subtilis and Bacillus amyloliquefaciens were respectively inoculated in the first culture medium under aseptic conditions, and the content of each component in 1.0L distilled water in the first culture medium was: peptone 10.0g, beef extract 3.0 g, sodium chloride 5.0g, the pH value is adjusted to 7.0, 33 ℃ under single strain culture 48 hours, obtain Bacillus subtilis seed culture liquid and Bacillus amyloliquefaciens seed culture liquid respectively, and viable count is 5.5 * 109cfu / ml;

[0052] b. Inoculate Rhodopseudomonas palustris into the liquid medium under aseptic conditions, and the content of each component in 1.0L distilled water in the second medium is: sodium bicarbonate 2.0g, sodium acetate 2.0g , ammonium chloride 1.0g, dipotassium hydrogen phosphate 0.5g, sodium chloride 12.0g, magnesium chloride 0.2g, yeast extract 0.5g, corn steep liquor 0.8g, pH value adjusted to 6.8, ...

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Abstract

The invention discloses a microorganism preparation for improving cultivation water. The microorganism preparation is characterized in that raw materials include 20-40% of bacillus subtilis, 20-40% of bacillus amyloliquefaciens, 20-50% of saccharomyces boulardii and 5-10% of rhodopseudom onas palustrisand. A preparation method comprises the steps of: respectively inoculating the bacillus subtilis, the bacillus amyloliquefaciens, the saccharomyces boulardii and the rhodopseudom onas palustrisand into corresponding culture media to obtain seed culture solutions; inoculating the seed culture solutions into the culture media according to proportion in sequence for fermentation, and performing high-speed centrifugation to obtain high-concentration bacilli liquid; transferring the bacilli liquid into a matrix, mixing, granulating, drying and crushing. After the microorganism preparation disclosed by the invention is used, the cultivation water becomes clear, water change amount is reduced remarkably, water resources are saved, and pollution caused by outward emission is reduced. Due to improvement of the quality of a water environment, drugs used for aquaculture products are reduced, antibiotic pollution is reduced, the food safety requirement is met, and product quality is ensured.

Description

technical field [0001] The invention relates to a microbial preparation for improving aquaculture water and a preparation method thereof, belonging to the technical field of aquaculture. Background technique [0002] With the vigorous development of aquaculture intensification in our country, the pollution of aquaculture water environment is becoming more and more serious, and the aquaculture ecological environment is not good, which greatly troubles the development of aquaculture industry. After a long period of high-intensity farming in the pond, the bottom substrate is aging, the water quality condition is declining, bacteria are overgrown, and the ecological environment is deteriorating. In the past, the measures to control the deterioration of water quality and bottom quality were firstly to change the water during the breeding process, and secondly to carry out pond dredging after harvesting. Water change and dredging have caused serious pollution to the breeding envi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C02F3/34C12R1/125C12R1/07C12R1/01C12R1/865C02F103/20
Inventor 江瀚马正驰
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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