Boulardii active dry yeasts and production method thereof

A technology of active dry yeast and Brady's yeast, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., and can solve problems such as affecting the application effect, easy death of Brady's yeast cells, and inconvenient transportation , to achieve the effect of overcoming the difficulty of drying, low production cost and high live cell rate

Active Publication Date: 2013-10-30
ANGELYEAST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the storage and transportation of its preparations, Brady's yeast cells are easy to die, which affects its application effect
Therefore, inconvenient transportation has become a bottleneck restricting the development of Brady's yeast

Method used

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  • Boulardii active dry yeasts and production method thereof
  • Boulardii active dry yeasts and production method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0119] 1) Slant-plane strain cultivation: inoculate the Brady's yeast strain prepared in the preparation example on a solid slope of wort agar with a sugar content of 5% by weight and a pH value of 4, and cultivate it at 26°C for 20 hours to obtain Inclined yeast strains;

[0120] 2) First-level liquid seed F bottle cultivation: Inoculate 1 loop of the slant yeast strain of step 1) into 100 ml of wort F bottle with a sugar content of 5% by weight and a pH value of 4, and cultivate at 26°C After 20 hours, about 100ml of primary liquid seed culture solution was obtained;

[0121] 3) secondary liquid seed Karl Fischer tank culture: step 2) the primary liquid seed culture solution is inoculated into 10 L of wort with a sugar content of 5% by weight, and a pH value of 4 in a Karl Fischer tank. Cultivate at 26°C for 20 hours to obtain about 10L of secondary liquid seed culture solution;

[0122] 4) Fermentation tank seed culture: inoculate the secondary liquid seed culture solutio...

Embodiment 2

[0130] 1) Slant-plane strain cultivation: Inoculate the Brady's yeast strain prepared in the preparation example on a solid slope of wort agar with a sugar content of 20% by weight and a pH value of 5, and cultivate it at 32°C for 48 hours to obtain Inclined yeast strains;

[0131] 2) First-level liquid seed F bottle cultivation: Inoculate 6 loops of the slant yeast strain of step 1) into 250 wort F bottles with a sugar content of 20% by weight and a pH value of 5, and cultivate at 32°C After 48 hours, about 250ml of primary liquid seed culture solution was obtained;

[0132] 3) two-stage liquid seed Karl Fischer jar culture: the first-stage liquid seed culture solution of step 2) is inoculated into the sugarcane molasses with a sugar content of 20% by weight that is equipped with 20L, and the pH value is 5 in a Karl Fischer jar. Cultivate at 32°C for 36 hours to obtain a secondary liquid seed culture solution;

[0133] 4) Seed culture in fermenter: Inoculate the secondary l...

Embodiment 3

[0141] 1) Slant-plane strain cultivation: Inoculate the Brady's yeast strain prepared in the preparation example on a solid slant of wort agar with a sugar content of 10% by weight and a pH value of 6, and cultivate it at 30°C for 36 hours to obtain Inclined yeast strains;

[0142] 2) First-level liquid seed F bottle cultivation: inoculate 4 rings of the slant yeast strain of step 1) into 500 ml of wort F bottle with a sugar content of 10% by weight and a pH value of 6, and cultivate at 30°C After 36 hours, about 500ml of primary liquid seed culture solution was obtained;

[0143] 3) two-stage liquid seed Karst tank culture: the first-stage liquid seed culture solution of step 2) is inoculated into the mixture of wort and sugar beet molasses with a sugar content of 40L, and the pH value is 6. Cultivate at 30°C for 30 hours in a jar to obtain a secondary liquid seed culture solution;

[0144] 4) Fermentation tank seed culture: inoculate the secondary liquid seed culture solut...

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Abstract

The invention provides boulardii active dry yeasts, wherein the total amount of cells of the dry yeasts is 30-75 billions/g, the rate of living cells is 50-88%, and the water content is 4-6%. A production method of the boulardii active dry yeasts comprises the following steps of: (1) culturing strains: inoculating and culturing Saccharomyces boulardii strains; (2) culturing seeds in a fermentation tank; (3) ventilating air flows into the fermentation tank for culture, after 20-28 hours of fermentation, adjusting the pH value of fermentation liquor to be 6.1-6.4, performing cell decolorization to ensure that the color of the product is lighter, and in 1.5-3 hours after the pH value is adjusted to be 6.1-6.4, raising the temperature of the fermentation liquor to 30-35 DEG C to perform fermentation, and adding sodium chloride at the same time; (4) separating; (5) adding an emulsifying agent and vegetable oil, and then granulating and molding; and (6) drying to obtain yeast particles.

Description

technical field [0001] The invention relates to a Brady active dry yeast, in particular to a production method of Brady active dry yeast. Background technique [0002] Saccharomyboulardii, also known as Saccharomyces boulardii and Saccharomyces boulardii, was first isolated from litchi in Indonesia. It is a subspecies of Saccharomyces cerevisiae and has been widely used in human antidiarrheal drugs . Its preparations are used in animal husbandry, which can effectively reduce the concentration of pathogenic bacteria and related toxins. It can also strengthen the micro-ecological balance and stimulate the immune system. Its application as a feed additive has been recognized by many countries in the world including China and the European Union. However, during the storage and transportation of its preparations, Brady's yeast cells are easy to die, which affects its application effect. Therefore, inconvenient transportation has become a bottleneck restricting the development o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/04C12R1/85
Inventor 郑国斌俞学锋李知洪余明华姚鹃池保国王健
Owner ANGELYEAST CO LTD
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